{"id":"https://openalex.org/W3138043523","doi":"https://doi.org/10.3389/fmicb.2021.633489","title":"Gene Expression, Biochemical Characterization of a sn-1, 3 Extracellular Lipase From Aspergillus niger GZUF36 and Its Model-Structure Analysis","display_name":"Gene Expression, Biochemical Characterization of a sn-1, 3 Extracellular Lipase From Aspergillus niger GZUF36 and Its Model-Structure Analysis","publication_year":2021,"publication_date":"2021-03-12","ids":{"openalex":"https://openalex.org/W3138043523","doi":"https://doi.org/10.3389/fmicb.2021.633489","mag":"3138043523","pmid":"https://pubmed.ncbi.nlm.nih.gov/33776965","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7994357"},"language":"en","primary_location":{"id":"doi:10.3389/fmicb.2021.633489","doi":"https://doi.org/10.3389/fmicb.2021.633489","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2021.633489","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.633489/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.633489/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064183060","display_name":"Shuqi Xing","orcid":"https://orcid.org/0009-0008-9528-4857","observed_orcids":["https://orcid.org/0009-0008-9528-4857"]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shuqi Xing","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ruonan Zhu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ruonan Zhu","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Kai Cheng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Kai Cheng","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113932601","display_name":"Yangyang Cai","orcid":"https://orcid.org/0009-0008-6273-0726","observed_orcids":["https://orcid.org/0009-0008-6273-0726"]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yangyang Cai","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049818027","display_name":"Yuedan Hu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yuedan Hu","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103261341","display_name":"Cuiqin Li","orcid":"https://orcid.org/0000-0002-1438-9171","observed_orcids":["https://orcid.org/0000-0002-1438-9171"]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Cuiqin Li","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","School of Chemistry and Chemical Engineering, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","School of Chemistry and Chemical Engineering, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"School of Chemistry and Chemical Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"School of Chemistry and Chemical Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050363785","display_name":"Xuefeng Zeng","orcid":"https://orcid.org/0000-0002-8656-2252","observed_orcids":["https://orcid.org/0000-0002-8656-2252"]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xuefeng Zeng","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085654103","display_name":"Qiujin Zhu","orcid":"https://orcid.org/0000-0002-0392-4992","observed_orcids":["https://orcid.org/0000-0002-0392-4992"]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiujin Zhu","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019645252","display_name":"Laping He","orcid":"https://orcid.org/0000-0002-3523-0872","observed_orcids":["https://orcid.org/0000-0002-3523-0872"]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Laping He","raw_affiliation_strings":["College of Liquor and Food Engineering, Guizhou University, Guiyang, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","College of Liquor and Food Engineering, Guizhou University, China","Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"College of Liquor and Food Engineering, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, China","institution_ids":["https://openalex.org/I178232147"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5019645252"],"corresponding_institution_ids":["https://openalex.org/I178232147"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":0.7007,"has_fulltext":true,"cited_by_count":21,"citation_normalized_percentile":{"value":0.6288642,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"12","issue":null,"first_page":"633489","last_page":"633489"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.6845999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.6845999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.29660001397132874,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.010300000198185444,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aspergillus-niger","display_name":"Aspergillus niger","score":0.8600000143051147},{"id":"https://openalex.org/keywords/pichia-pastoris-expression-system","display_name":"Pichia pastoris expression","score":0.9279999732971191},{"id":"https://openalex.org/keywords/ultrasound-assisted-synthesis","display_name":"sonochemical synthesis","score":0.8029999732971191},{"id":"https://openalex.org/keywords/alpha-beta-hydrolase-fold","display_name":"alpha/beta hydrolase fold","score":0.8320000171661377},{"id":"https://openalex.org/keywords/catalytic-residues","display_name":"catalytic residues","score":0.7860000133514404},{"id":"https://openalex.org/keywords/lipase-thermostability","display_name":"lipase thermostability","score":0.5640000104904175},{"id":"https://openalex.org/keywords/metal-ion-inhibition","display_name":"metal ion inhibition","score":0.6119999885559082},{"id":"https://openalex.org/keywords/homology-modeling","display_name":"homology modeling","score":0.6439999938011169},{"id":"https://openalex.org/keywords/small-angle-x-ray-scattering","display_name":"small-angle X-ray scattering","score":0.8949999809265137}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.8859094977378845},{"id":"https://openalex.org/C2775960407","wikidata":"https://www.wikidata.org/wiki/Q132014","display_name":"Aspergillus niger","level":2,"score":0.8210185170173645},{"id":"https://openalex.org/C2779697368","wikidata":"https://www.wikidata.org/wiki/Q380967","display_name":"Lipase","level":3,"score":0.7072775363922119},{"id":"https://openalex.org/C169627665","wikidata":"https://www.wikidata.org/wiki/Q5767913","display_name":"Homology modeling","level":3,"score":0.6179056167602539},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5530906915664673},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.4167838990688324},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3793395459651947},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.35202163457870483},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29909080266952515},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17438280582427979},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.09151479601860046}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3389/fmicb.2021.633489","doi":"https://doi.org/10.3389/fmicb.2021.633489","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2021.633489","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.633489/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},{"id":"pmid:33776965","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33776965","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7994357","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC7994357/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Microbiol","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:9b72ca0f574b45c28998da90fc2018da","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/9b72ca0f574b45c28998da90fc2018da","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Microbiology, Vol 12 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fmicb.2021.633489","doi":"https://doi.org/10.3389/fmicb.2021.633489","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2021.633489","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.633489/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2415212033","display_name":null,"funder_award_id":"2018-5781","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G5121424795","display_name":null,"funder_award_id":"QKHPTRC [2020] 5004","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G6070127386","display_name":null,"funder_award_id":"31660010","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G8262899091","display_name":"Sn-2\u9ed1\u66f2\u9709CCTCC No. M2012538\u8102\u80aa\u9176\u7684\u7ed3\u6784\u4e0e\u529f\u80fd\u7814\u7a76","funder_award_id":"31870002","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321927","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3138043523.pdf","grobid_xml":"https://content.openalex.org/works/W3138043523.grobid-xml"},"referenced_works_count":71,"referenced_works":["https://openalex.org/W95014119","https://openalex.org/W1031184221","https://openalex.org/W1503820853","https://openalex.org/W1551390803","https://openalex.org/W1951832557","https://openalex.org/W1964556874","https://openalex.org/W1979503732","https://openalex.org/W1980317108","https://openalex.org/W1982126789","https://openalex.org/W1986191025","https://openalex.org/W1991890196","https://openalex.org/W1992191979","https://openalex.org/W1992192767","https://openalex.org/W2014092023","https://openalex.org/W2033793590","https://openalex.org/W2036414218","https://openalex.org/W2050091361","https://openalex.org/W2061904682","https://openalex.org/W2063212547","https://openalex.org/W2063968553","https://openalex.org/W2064127883","https://openalex.org/W2067431380","https://openalex.org/W2067956654","https://openalex.org/W2071486470","https://openalex.org/W2072435127","https://openalex.org/W2074473216","https://openalex.org/W2082581989","https://openalex.org/W2084426793","https://openalex.org/W2089631717","https://openalex.org/W2091464788","https://openalex.org/W2102993309","https://openalex.org/W2105668062","https://openalex.org/W2110218621","https://openalex.org/W2120257484","https://openalex.org/W2124983865","https://openalex.org/W2137015675","https://openalex.org/W2149885731","https://openalex.org/W2156469184","https://openalex.org/W2164361934","https://openalex.org/W2167262253","https://openalex.org/W2168974130","https://openalex.org/W2169678694","https://openalex.org/W2320310848","https://openalex.org/W2323720159","https://openalex.org/W2331441635","https://openalex.org/W2489181495","https://openalex.org/W2528176026","https://openalex.org/W2566705229","https://openalex.org/W2591967910","https://openalex.org/W2600629535","https://openalex.org/W2610169850","https://openalex.org/W2766774346","https://openalex.org/W2790038365","https://openalex.org/W2890796532","https://openalex.org/W2901076315","https://openalex.org/W2939568161","https://openalex.org/W2944043225","https://openalex.org/W2945401962","https://openalex.org/W2955334504","https://openalex.org/W2984563477","https://openalex.org/W3008259506","https://openalex.org/W3011499653","https://openalex.org/W3022464053","https://openalex.org/W3022698926","https://openalex.org/W3033728283","https://openalex.org/W3119859962","https://openalex.org/W4232534952","https://openalex.org/W4234451110","https://openalex.org/W4244821183","https://openalex.org/W4251110453","https://openalex.org/W4388005167"],"related_works":["https://openalex.org/W2084426793","https://openalex.org/W3008259506","https://openalex.org/W3197233251","https://openalex.org/W2890796532","https://openalex.org/W3126537150","https://openalex.org/W4247909925","https://openalex.org/W4297920812","https://openalex.org/W2048130627","https://openalex.org/W2922403274","https://openalex.org/W2379263230"],"abstract_inverted_index":{"In":[0],"this":[1,231],"study,":[2],"a":[3,96,211],"sn-1,":[4],"3":[5],"extracellular":[6],"lipases":[7],"from":[8,230],"Aspergillus":[9],"niger":[10,217],"GZUF36":[11],"(PEXANL1)":[12],"was":[13,24,128],"expressed":[14],"in":[15,77,182,195,243],"Pichia":[16],"pastoris,":[17],"characterized,":[18],"and":[19,53,68,71,93,102,142,167,175,223],"the":[20,35,62,78,81,104,124,183,190,228,235,244],"predicted":[21],"structural":[22,213],"model":[23,200,214],"analyzed.":[25],"The":[26,58,113,153,197],"optimized":[27],"culture":[28],"conditions":[29],"of":[30,39,80,98,110,115,120,126,136,150,156,178,193,201,215,238],"P.":[31],"pastoris":[32],"showed":[33,122,161],"that":[34,123,172],"highest":[36,63],"lipase":[37,218,241],"activity":[38,64,74,109],"66.5":[40],"\u00b1":[41],"1.4":[42],"U/mL":[43],"(P":[44,139],"<":[45,140],"0.05)":[46,141],"could":[47],"be":[48],"attained":[49],"with":[50,133,147,204],"1%":[51],"methanol":[52],"96":[54],"h":[55],"induction":[56],"time.":[57],"purified":[59,111],"PEXANL1":[60,117,157,179,194,202],"exhibited":[61],"at":[65,95],"pH":[66],"4.0":[67],"40\u00b0C":[69],"temperature,":[70],"its":[72,205],"original":[73],"remained":[75],"unaltered":[76],"majority":[79],"organic":[82],"solvents":[83],"(20%":[84],"v/v":[85],"concentration).":[86],"Triton":[87],"X-100,":[88],"Tween":[89,91],"20,":[90],"80,":[92],"SDS":[94],"concentration":[97],"0.01%":[99],"(w/v)":[100],"enhanced,":[101],"all":[103],"metal":[105],"ions":[106],"tested":[107],"inhibited":[108],"PEXANL.":[112],"results":[114,170],"ultrasound-assisted":[116],"catalyzed":[118],"synthesis":[119],"1,3-diaglycerides":[121],"content":[125],"1,3-diglycerides":[127],"rapidly":[129],"increased":[130],"to":[131,145],"36.90%":[132],"25":[134],"min":[135,149],"ultrasound":[137,151],"duration":[138],"later":[143],"decreased":[144],"19.93%":[146],"35":[148],"duration.":[152],"modeled":[154],"structure":[155],"by":[158],"comparative":[159],"modeling":[160,206,222],"\u03b1/\u03b2":[162],"hydrolase":[163],"fold.":[164],"Structural":[165],"superposition":[166],"molecular":[168],"docking":[169],"validated":[171],"Ser162,":[173],"His274,":[174],"Asp217":[176],"residues":[177],"were":[180],"involved":[181],"catalysis.":[184],"Small-angle":[185],"X-ray":[186,225],"scattering":[187],"analysis":[188],"indicated":[189],"monomer":[191],"properties":[192],"solution.":[196],"ab":[198],"initio":[199],"overlapped":[203],"structure.":[207],"This":[208],"work":[209],"presents":[210],"reliable":[212],"A.":[216],"based":[219],"on":[220],"homology":[221],"small-angle":[224],"scattering.":[226],"Besides,":[227],"data":[229],"study":[232],"will":[233],"benefit":[234],"rational":[236],"design":[237],"suitable":[239],"crystalline":[240],"variants":[242],"future.":[245]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2021-03-29T00:00:00"}