{"id":"https://openalex.org/W3135851561","doi":"https://doi.org/10.3389/fbioe.2021.618259","title":"Optimizing a Fed-Batch High-Density Fermentation Process for Medium Chain-Length Poly(3-Hydroxyalkanoates) in Escherichia coli","display_name":"Optimizing a Fed-Batch High-Density Fermentation Process for Medium Chain-Length Poly(3-Hydroxyalkanoates) in Escherichia coli","publication_year":2021,"publication_date":"2021-02-26","ids":{"openalex":"https://openalex.org/W3135851561","doi":"https://doi.org/10.3389/fbioe.2021.618259","mag":"3135851561","pmid":"https://pubmed.ncbi.nlm.nih.gov/33718339","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7953831"},"language":"en","primary_location":{"id":"doi:10.3389/fbioe.2021.618259","doi":"https://doi.org/10.3389/fbioe.2021.618259","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbioe.2021.618259","pdf_url":"https://www.frontiersin.org/articles/10.3389/fbioe.2021.618259/pdf","source":{"id":"https://openalex.org/S2595131146","display_name":"Frontiers in Bioengineering and Biotechnology","issn_l":"2296-4185","issn":["2296-4185"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioengineering and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fbioe.2021.618259/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088410892","display_name":"Ryan A. Scheel","orcid":"https://orcid.org/0000-0003-0792-9958","observed_orcids":["https://orcid.org/0000-0003-0792-9958"]},"institutions":[{"id":"https://openalex.org/I157349981","display_name":"SUNY College of Environmental Science and Forestry","ror":"https://ror.org/00qv0tw17","country_code":"US","type":"education","lineage":["https://openalex.org/I157349981"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ryan A. Scheel","raw_affiliation_strings":["Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","institution_ids":["https://openalex.org/I157349981"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015237010","display_name":"Truong Ho","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157349981","display_name":"SUNY College of Environmental Science and Forestry","ror":"https://ror.org/00qv0tw17","country_code":"US","type":"education","lineage":["https://openalex.org/I157349981"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Truong Ho","raw_affiliation_strings":["Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","institution_ids":["https://openalex.org/I157349981"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072010168","display_name":"Yuki Kageyama","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157349981","display_name":"SUNY College of Environmental Science and Forestry","ror":"https://ror.org/00qv0tw17","country_code":"US","type":"education","lineage":["https://openalex.org/I157349981"]},{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Yuki Kageyama","raw_affiliation_strings":["Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","Division of Applied Chemistry, Department of Engineering, Hokkaido University, Sapporo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","institution_ids":["https://openalex.org/I157349981"]},{"raw_affiliation_string":"Division of Applied Chemistry, Department of Engineering, Hokkaido University, Sapporo, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064849556","display_name":"Jessica Masisak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157349981","display_name":"SUNY College of Environmental Science and Forestry","ror":"https://ror.org/00qv0tw17","country_code":"US","type":"education","lineage":["https://openalex.org/I157349981"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jessica Masisak","raw_affiliation_strings":["Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","institution_ids":["https://openalex.org/I157349981"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082715081","display_name":"Seamus McKenney","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157349981","display_name":"SUNY College of Environmental Science and Forestry","ror":"https://ror.org/00qv0tw17","country_code":"US","type":"education","lineage":["https://openalex.org/I157349981"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Seamus McKenney","raw_affiliation_strings":["Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","institution_ids":["https://openalex.org/I157349981"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075448607","display_name":"Benjamin R. Lundgren","orcid":"https://orcid.org/0000-0002-6556-120X","observed_orcids":["https://orcid.org/0000-0002-6556-120X"]},"institutions":[{"id":"https://openalex.org/I157349981","display_name":"SUNY College of Environmental Science and Forestry","ror":"https://ror.org/00qv0tw17","country_code":"US","type":"education","lineage":["https://openalex.org/I157349981"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benjamin R. Lundgren","raw_affiliation_strings":["Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","institution_ids":["https://openalex.org/I157349981"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043701519","display_name":"Christopher T. Nomura","orcid":"https://orcid.org/0000-0001-6981-5679","observed_orcids":["https://orcid.org/0000-0001-6981-5679"]},"institutions":[{"id":"https://openalex.org/I155093810","display_name":"University of Idaho","ror":"https://ror.org/03hbp5t65","country_code":"US","type":"education","lineage":["https://openalex.org/I155093810"]},{"id":"https://openalex.org/I157349981","display_name":"SUNY College of Environmental Science and Forestry","ror":"https://ror.org/00qv0tw17","country_code":"US","type":"education","lineage":["https://openalex.org/I157349981"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Christopher T. Nomura","raw_affiliation_strings":["Department of Biological Sciences, College of Science, University of Idaho, Moscow, ID, United States","Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, College of Science, University of Idaho, Moscow, ID, United States","institution_ids":["https://openalex.org/I155093810"]},{"raw_affiliation_string":"Department of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, NY, United States","institution_ids":["https://openalex.org/I157349981"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5043701519","https://openalex.org/A5088410892"],"corresponding_institution_ids":["https://openalex.org/I155093810","https://openalex.org/I157349981"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":0.9801,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.66290359,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"9","issue":null,"first_page":"618259","last_page":"618259"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10661","display_name":"biodegradable polymer synthesis and properties","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10661","display_name":"biodegradable polymer synthesis and properties","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T14285","display_name":"Biopolymer Synthesis and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polyhydroxyalkanoates","display_name":"polyhydroxyalkanoates","score":0.9459999799728394},{"id":"https://openalex.org/keywords/medium-chain-length-polyhydroxyalkanoates","display_name":"medium-chain-length polyhydroxyalkanoates","score":0.871999979019165},{"id":"https://openalex.org/keywords/poly-3-hydroxyoctanoate","display_name":"poly(3-hydroxyoctanoate)","score":0.8320000171661377},{"id":"https://openalex.org/keywords/copolymer-production","display_name":"copolymer production","score":0.5360000133514404},{"id":"https://openalex.org/keywords/decanoic-acid","display_name":"capric acid","score":0.6499999761581421},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7450000047683716},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.8610000014305115},{"id":"https://openalex.org/keywords/high-cell-density-fermentation","display_name":"high cell density fermentation","score":0.7409999966621399},{"id":"https://openalex.org/keywords/biodegradable-polymers","display_name":"biodegradable polymers","score":0.6899999976158142},{"id":"https://openalex.org/keywords/polymer-composition-control","display_name":"polymer composition control","score":0.4740000069141388}],"concepts":[{"id":"https://openalex.org/C2776950135","wikidata":"https://www.wikidata.org/wiki/Q422613","display_name":"Decanoic acid","level":2,"score":0.9397597312927246},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.698811411857605},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6099206209182739},{"id":"https://openalex.org/C73447357","wikidata":"https://www.wikidata.org/wiki/Q425079","display_name":"Polyhydroxyalkanoates","level":3,"score":0.5864481329917908},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5748171806335449},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.5689150094985962},{"id":"https://openalex.org/C2778471300","wikidata":"https://www.wikidata.org/wiki/Q422597","display_name":"Hexanoic acid","level":2,"score":0.5246976613998413},{"id":"https://openalex.org/C115540264","wikidata":"https://www.wikidata.org/wiki/Q2945560","display_name":"Biomass (ecology)","level":2,"score":0.49087315797805786},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.46777239441871643},{"id":"https://openalex.org/C543025807","wikidata":"https://www.wikidata.org/wiki/Q61476","display_name":"Fatty acid","level":2,"score":0.44082748889923096},{"id":"https://openalex.org/C167431671","wikidata":"https://www.wikidata.org/wiki/Q372004","display_name":"Fed-batch culture","level":3,"score":0.4217771887779236},{"id":"https://openalex.org/C119394753","wikidata":"https://www.wikidata.org/wiki/Q188245","display_name":"Polyester","level":2,"score":0.4178021550178528},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.39048105478286743},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3854812979698181},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3715476393699646},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.26421409845352173},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.22338947653770447},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19229942560195923},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3389/fbioe.2021.618259","doi":"https://doi.org/10.3389/fbioe.2021.618259","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbioe.2021.618259","pdf_url":"https://www.frontiersin.org/articles/10.3389/fbioe.2021.618259/pdf","source":{"id":"https://openalex.org/S2595131146","display_name":"Frontiers in Bioengineering and Biotechnology","issn_l":"2296-4185","issn":["2296-4185"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioengineering and Biotechnology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7953831","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7953831","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Bioeng Biotechnol","raw_type":"Text"},{"id":"pmid:33718339","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33718339","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in bioengineering and biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:f8d8b7378c7f4843a19a5bf88a07e5d2","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/f8d8b7378c7f4843a19a5bf88a07e5d2","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fbioe.2021.618259","doi":"https://doi.org/10.3389/fbioe.2021.618259","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbioe.2021.618259","pdf_url":"https://www.frontiersin.org/articles/10.3389/fbioe.2021.618259/pdf","source":{"id":"https://openalex.org/S2595131146","display_name":"Frontiers in Bioengineering and Biotechnology","issn_l":"2296-4185","issn":["2296-4185"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioengineering and Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12","score":0.5925999879837036}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5760201950","display_name":null,"funder_award_id":"CBET 1263905","funder_id":"https://openalex.org/F4320337390","funder_display_name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems"},{"id":"https://openalex.org/G8339816354","display_name":"SusChEM: Engineering E. coli for improved production of polyhydroxyalkanoate (PHA)-based biodegradable plastics","funder_award_id":"1263905","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G906614111","display_name":null,"funder_award_id":"1263905","funder_id":"https://openalex.org/F4320337390","funder_display_name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320337390","display_name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","ror":"https://ror.org/0471zv972"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3135851561.pdf","grobid_xml":"https://content.openalex.org/works/W3135851561.grobid-xml"},"referenced_works_count":57,"referenced_works":["https://openalex.org/W181452442","https://openalex.org/W1455416950","https://openalex.org/W1492012122","https://openalex.org/W1571667468","https://openalex.org/W1602014918","https://openalex.org/W1648877543","https://openalex.org/W1781531090","https://openalex.org/W1970561846","https://openalex.org/W1996835066","https://openalex.org/W2002683215","https://openalex.org/W2004157148","https://openalex.org/W2006851992","https://openalex.org/W2009220402","https://openalex.org/W2020857985","https://openalex.org/W2022640080","https://openalex.org/W2023000402","https://openalex.org/W2024835614","https://openalex.org/W2038683612","https://openalex.org/W2039371539","https://openalex.org/W2044038933","https://openalex.org/W2051266642","https://openalex.org/W2051511924","https://openalex.org/W2068624029","https://openalex.org/W2077263483","https://openalex.org/W2086652584","https://openalex.org/W2091835118","https://openalex.org/W2093136988","https://openalex.org/W2103267495","https://openalex.org/W2116137883","https://openalex.org/W2118589039","https://openalex.org/W2120211414","https://openalex.org/W2125844555","https://openalex.org/W2128554689","https://openalex.org/W2129162353","https://openalex.org/W2129941226","https://openalex.org/W2137304870","https://openalex.org/W2143567887","https://openalex.org/W2148033653","https://openalex.org/W2151463520","https://openalex.org/W2155857889","https://openalex.org/W2157190495","https://openalex.org/W2158479639","https://openalex.org/W2167149191","https://openalex.org/W2257307527","https://openalex.org/W2313991536","https://openalex.org/W2409407070","https://openalex.org/W2512626975","https://openalex.org/W2556609263","https://openalex.org/W2762894418","https://openalex.org/W2767080254","https://openalex.org/W2792590822","https://openalex.org/W2804007336","https://openalex.org/W2898303110","https://openalex.org/W2902452529","https://openalex.org/W2989865496","https://openalex.org/W2998678429","https://openalex.org/W4210686916"],"related_works":["https://openalex.org/W2191153872","https://openalex.org/W2902452529","https://openalex.org/W4230802005","https://openalex.org/W30112896","https://openalex.org/W2033829567","https://openalex.org/W2028873514","https://openalex.org/W2762894418","https://openalex.org/W2895155943","https://openalex.org/W2137304870","https://openalex.org/W2550067449"],"abstract_inverted_index":{"Production":[0],"of":[1,15,25,69,100,151,160,170,192,212,221,282,298],"medium":[2],"chain-length":[3],"poly(3-hydroxyalkanoates)":[4],"[PHA]":[5],"polymers":[6,33,55],"with":[7,34,97,134,149,177,200,261],"tightly":[8],"defined":[9,37],"compositions":[10,38],"is":[11,66],"an":[12,178,201],"important":[13],"area":[14],"research":[16,70],"to":[17,50,58,175,216,266,269,318],"expand":[18],"the":[19,23,98,137,190,197,296,304],"application":[20],"and":[21,29,47,64,85,141,153,164,247,258,286,307],"improve":[22],"properties":[24,42],"these":[26,54,313],"promising":[27],"biobased":[28],"biodegradable":[30],"materials.":[31],"PHA":[32,143,320],"homopolymeric":[35],"or":[36],"exhibit":[39],"attractive":[40],"material":[41],"such":[43],"as":[44,115,136],"increased":[45],"flexibility":[46],"elasticity":[48],"relative":[49],"poly(3-hydroxybutyrate)":[51],"[PHB];":[52],"however,":[53],"are":[56],"difficult":[57],"biosynthesize":[59],"in":[60,91,124,189,203,206,295],"native":[61],"PHA-producing":[62],"organisms,":[63],"there":[65],"a":[67,87,92,116,127,142,154,207,219],"paucity":[68],"toward":[71],"developing":[72],"high-density":[73],"cultivation":[74],"methods":[75,314],"while":[76],"retaining":[77],"compositional":[78],"control.":[79],"In":[80],"this":[81,276],"study,":[82],"we":[83],"developed":[84],"optimized":[86],"fed-batch":[88],"fermentation":[89,198],"process":[90],"stirred":[93],"tank":[94],"reactor,":[95],"beginning":[96],"biosynthesis":[99,144],"poly(3-hydroxydecanoate)":[101],"[PHD]":[102],"from":[103,264],"decanoic":[104,171,193,209],"acid":[105,172,194,210,249,280,285,289],"by":[106],"\u03b2-oxidation":[107],"deficient":[108],"recombinant":[109],"Escherichia":[110],"coli":[111],"LSBJ":[112],"using":[113],"glucose":[114,135,152,165],"co-substrate":[117],"solely":[118],"for":[119],"growth.":[120],"Bacteria":[121],"were":[122,250],"cultured":[123],"two":[125],"stages,":[126],"biomass":[128],"accumulation":[129],"stage":[130,145],"(37\u00b0C,":[131],"pH":[132,147],"7.0)":[133],"primary":[138],"carbon":[139],"source":[140],"(30\u00b0C,":[146],"8.0)":[148],"co-feeding":[150],"fatty":[155],"acid.":[156],"Through":[157],"iterative":[158],"optimizations":[159],"semi-defined":[161],"media":[162],"composition":[163],"feed":[166,211],"rate,":[167],"6.0":[168],"g":[169,183,214,224,229,256,271],"was":[173],"converted":[174,215],"PHD":[176,217],"87.5%":[179],"molar":[180],"yield":[181,220],"(4.54":[182],"L":[184,225,230,272],"\u20131":[185,226,231,233,273],").":[186,274],"Stepwise":[187],"increases":[188],"amount":[191],"fed":[195],"during":[196],"correlated":[199],"increase":[202],"PHD,":[204],"resulting":[205],"final":[208],"25":[213],"at":[218,235,254,302,308],"89.4%":[222],"(20.1":[223],",":[227],"0.42":[228],"h":[232],"),":[234],"which":[236],"point":[237],"foaming":[238],"became":[239],"uncontrollable.":[240],"Hexanoic":[241],"acid,":[242,244,246],"octanoic":[243,283,287],"10-undecenoic":[245],"10-bromodecanoic":[248],"all":[251],"individually":[252],"supplemented":[253],"20":[255],"each":[257],"successfully":[259],"polymerized":[260],"yields":[262],"ranging":[263],"66.8":[265],"99.0%":[267],"(9.24":[268],"18.2":[270],"Using":[275],"bioreactor":[277],"strategy,":[278],"co-fatty":[279],"feeds":[281],"acid/decanoic":[284],"acid/10-azidodecanoic":[288],"(8:2":[290],"mol":[291],"ratio":[292,306],"each)":[293],"resulted":[294],"production":[297],"their":[299],"respective":[300],"copolymers":[301],"nearly":[303],"same":[305],"high":[309],"yield,":[310],"demonstrating":[311],"that":[312],"can":[315],"be":[316],"used":[317],"control":[319],"copolymer":[321],"composition.":[322]},"counts_by_year":[{"year":2026,"cited_by_count":10},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2021-03-15T00:00:00"}