{"id":"https://openalex.org/W3135581941","doi":"https://doi.org/10.3390/v13030420","title":"Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2","display_name":"Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2","publication_year":2021,"publication_date":"2021-03-05","ids":{"openalex":"https://openalex.org/W3135581941","doi":"https://doi.org/10.3390/v13030420","mag":"3135581941","pmid":"https://pubmed.ncbi.nlm.nih.gov/33807908","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7998296"},"language":"en","primary_location":{"id":"doi:10.3390/v13030420","doi":"https://doi.org/10.3390/v13030420","is_oa":true,"landing_page_url":"https://doi.org/10.3390/v13030420","pdf_url":"https://www.mdpi.com/1999-4915/13/3/420/pdf?version=1615171161","source":{"id":"https://openalex.org/S55776271","display_name":"Viruses","issn_l":"1999-4915","issn":["1999-4915"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Viruses","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1999-4915/13/3/420/pdf?version=1615171161","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5035911013","display_name":"Curti Lucia","orcid":"https://orcid.org/0000-0002-8367-7804","observed_orcids":["https://orcid.org/0000-0002-8367-7804"]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Luc\u00eda Ana Curti","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":"https://orcid.org/0000-0002-8367-7804","affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032883016","display_name":"Ivana Primost","orcid":"https://orcid.org/0000-0003-3907-9652","observed_orcids":["https://orcid.org/0000-0003-3907-9652"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ivana Primost","raw_affiliation_strings":["Genetics and Molecular Biology Laboratory, Hospital Municipal de Trauma y Emergencias Dr. Federico Abete, Malvinas Argentinas, Buenos Aires 1615, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genetics and Molecular Biology Laboratory, Hospital Municipal de Trauma y Emergencias Dr. Federico Abete, Malvinas Argentinas, Buenos Aires 1615, Argentina","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054892241","display_name":"S Valla","orcid":"https://orcid.org/0000-0001-5565-0391","observed_orcids":["https://orcid.org/0000-0001-5565-0391"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I3130568980","display_name":"National University of Northwestern Buenos Aires","ror":"https://ror.org/00c8e6e47","country_code":"AR","type":"education","lineage":["https://openalex.org/I3130568980"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Sofia Valla","raw_affiliation_strings":["CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","Centro de Investigaciones B\u00e1sicas y Aplicadas (CIBA), Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA), Universidad del Noroeste de la Provincia de Buenos Aires (UNNOBA), Jun\u00edn, Buenos Aires 6000, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","institution_ids":["https://openalex.org/I151201029"]},{"raw_affiliation_string":"Centro de Investigaciones B\u00e1sicas y Aplicadas (CIBA), Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA), Universidad del Noroeste de la Provincia de Buenos Aires (UNNOBA), Jun\u00edn, Buenos Aires 6000, Argentina","institution_ids":["https://openalex.org/I3130568980"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064237614","display_name":"Daiana M. Iba\u00f1ez Alegre","orcid":"https://orcid.org/0000-0002-0317-0393","observed_orcids":["https://orcid.org/0000-0002-0317-0393"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I17994240","display_name":"National University of Misiones","ror":"https://ror.org/03kr8x191","country_code":"AR","type":"education","lineage":["https://openalex.org/I17994240"]},{"id":"https://openalex.org/I4210146412","display_name":"Instituto de Biolog\u00eda Subtropical","ror":"https://ror.org/04jdqdr13","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I17994240","https://openalex.org/I4210104196","https://openalex.org/I4210123736","https://openalex.org/I4210146412","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Daiana Iba\u00f1ez Alegre","raw_affiliation_strings":["CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","Laboratorio Grupo de Investigaci\u00f3n en Gen\u00e9tica Aplicada (GIGA, FCEQyN), Instituto de Biolog\u00eda Subtropical, Universidad Nacional de Misiones (UNM\u2013CONICET), Posadas 3304, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","institution_ids":["https://openalex.org/I151201029"]},{"raw_affiliation_string":"Laboratorio Grupo de Investigaci\u00f3n en Gen\u00e9tica Aplicada (GIGA, FCEQyN), Instituto de Biolog\u00eda Subtropical, Universidad Nacional de Misiones (UNM\u2013CONICET), Posadas 3304, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I17994240","https://openalex.org/I4210146412"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091717921","display_name":"Cecilia Olguin Perglione","orcid":"https://orcid.org/0000-0003-0362-8883","observed_orcids":["https://orcid.org/0000-0003-0362-8883"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4210153948","display_name":"National Agricultural Technology Institute","ror":"https://ror.org/04wm52x94","country_code":"AR","type":"government","lineage":["https://openalex.org/I4210153948"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Cecilia Olguin Perglione","raw_affiliation_strings":["CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","Instituto de Virolog\u00eda, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Buenos Aires 1686, Argentina"],"raw_orcid":"https://orcid.org/0000-0003-0362-8883","affiliations":[{"raw_affiliation_string":"CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","institution_ids":["https://openalex.org/I151201029"]},{"raw_affiliation_string":"Instituto de Virolog\u00eda, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Buenos Aires 1686, Argentina","institution_ids":["https://openalex.org/I4210153948"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084275906","display_name":"Guillermo Daniel Repizo","orcid":"https://orcid.org/0000-0003-1318-8738","observed_orcids":["https://orcid.org/0000-0003-1318-8738"]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Guillermo Daniel Repizo","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":"https://orcid.org/0000-0003-1318-8738","affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086846481","display_name":"Julia Lara","orcid":"https://orcid.org/0000-0001-8303-5281","observed_orcids":["https://orcid.org/0000-0001-8303-5281"]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Julia Lara","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":"https://orcid.org/0000-0001-8303-5281","affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028493188","display_name":"Ivana Parcerisa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ivana Parcerisa","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057660584","display_name":"Antonela R. Palacios","orcid":"https://orcid.org/0000-0003-2904-7622","observed_orcids":["https://orcid.org/0000-0003-2904-7622"]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Antonela Palacios","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":"https://orcid.org/0000-0003-2904-7622","affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088994434","display_name":"Mar\u00eda\u2010Eugenia Llases","orcid":"https://orcid.org/0000-0003-0867-3405","observed_orcids":["https://orcid.org/0000-0003-0867-3405"]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mar\u00eda Eugenia Llases","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":"https://orcid.org/0000-0003-0867-3405","affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089929620","display_name":"Adriana Rinflerch","orcid":"https://orcid.org/0000-0002-1840-9993","observed_orcids":["https://orcid.org/0000-0002-1840-9993"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I17994240","display_name":"National University of Misiones","ror":"https://ror.org/03kr8x191","country_code":"AR","type":"education","lineage":["https://openalex.org/I17994240"]},{"id":"https://openalex.org/I4210146412","display_name":"Instituto de Biolog\u00eda Subtropical","ror":"https://ror.org/04jdqdr13","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I17994240","https://openalex.org/I4210104196","https://openalex.org/I4210123736","https://openalex.org/I4210146412","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Adriana Rinflerch","raw_affiliation_strings":["CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","Laboratorio Grupo de Investigaci\u00f3n en Gen\u00e9tica Aplicada (GIGA, FCEQyN), Instituto de Biolog\u00eda Subtropical, Universidad Nacional de Misiones (UNM\u2013CONICET), Posadas 3304, Argentina"],"raw_orcid":"https://orcid.org/0000-0002-1840-9993","affiliations":[{"raw_affiliation_string":"CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","institution_ids":["https://openalex.org/I151201029"]},{"raw_affiliation_string":"Laboratorio Grupo de Investigaci\u00f3n en Gen\u00e9tica Aplicada (GIGA, FCEQyN), Instituto de Biolog\u00eda Subtropical, Universidad Nacional de Misiones (UNM\u2013CONICET), Posadas 3304, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I17994240","https://openalex.org/I4210146412"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108276750","display_name":"Melanie Barrios","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24354313","display_name":"Universidad de Buenos Aires","ror":"https://ror.org/0081fs513","country_code":"AR","type":"education","lineage":["https://openalex.org/I24354313"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Melanie Barrios","raw_affiliation_strings":["Instituto de Producci\u00f3n Agropecuaria (INPA), Universidad de Buenos Aires, Buenos Aires 1053, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Producci\u00f3n Agropecuaria (INPA), Universidad de Buenos Aires, Buenos Aires 1053, Argentina","institution_ids":["https://openalex.org/I24354313"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026243032","display_name":"Federico Pereyra-Bonnet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Federico Pereyra Bonnet","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077116105","display_name":"Carla Alejandra Gim\u00e9nez","orcid":"https://orcid.org/0000-0003-3532-0891","observed_orcids":["https://orcid.org/0000-0003-3532-0891"]},"institutions":[{"id":"https://openalex.org/I4210146206","display_name":"Caspr Biotech","ror":"https://ror.org/04rpfqy30","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210146206"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Carla Alejandra Gimenez","raw_affiliation_strings":["CASPR Biotech, San Francisco, CA 94103, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CASPR Biotech, San Francisco, CA 94103, USA","institution_ids":["https://openalex.org/I4210146206"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004128024","display_name":"D\u00e9bora Natalia Marcone","orcid":"https://orcid.org/0000-0003-4407-7280","observed_orcids":["https://orcid.org/0000-0003-4407-7280"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I24354313","display_name":"Universidad de Buenos Aires","ror":"https://ror.org/0081fs513","country_code":"AR","type":"education","lineage":["https://openalex.org/I24354313"]}],"countries":["AR"],"is_corresponding":true,"raw_author_name":"D\u00e9bora Natalia Marcone","raw_affiliation_strings":["CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","Departamento de Microbiolog\u00eda, Inmunolog\u00eda, Biotecnolog\u00eda y Gen\u00e9tica, C\u00e1tedra de Virolog\u00eda, Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires 1113, Argentina"],"raw_orcid":"https://orcid.org/0000-0003-4407-7280","affiliations":[{"raw_affiliation_string":"CONICET-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires 1425, Argentina","institution_ids":["https://openalex.org/I151201029"]},{"raw_affiliation_string":"Departamento de Microbiolog\u00eda, Inmunolog\u00eda, Biotecnolog\u00eda y Gen\u00e9tica, C\u00e1tedra de Virolog\u00eda, Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires 1113, Argentina","institution_ids":["https://openalex.org/I24354313"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5004128024","https://openalex.org/A5077116105"],"corresponding_institution_ids":["https://openalex.org/I151201029","https://openalex.org/I24354313","https://openalex.org/I4210146206"],"apc_list":{"value":2200,"currency":"CHF","value_usd":2376},"apc_paid":{"value":2200,"currency":"CHF","value_usd":2376},"fwci":2.8919,"has_fulltext":true,"cited_by_count":45,"citation_normalized_percentile":{"value":0.92904067,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":99},"biblio":{"volume":"13","issue":"3","first_page":"420","last_page":"420"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10041","display_name":"COVID-19 Clinical Research Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sars-cov-2-detection","display_name":"COVID-19 detection","score":0.9330000281333923},{"id":"https://openalex.org/keywords/isothermal-amplification","display_name":"isothermal amplification","score":0.7699999809265137},{"id":"https://openalex.org/keywords/rt-qpcr-comparison","display_name":"RT-qPCR comparison","score":0.753000020980835},{"id":"https://openalex.org/keywords/nasopharyngeal-swab","display_name":"nasopharyngeal swab","score":0.8289999961853027},{"id":"https://openalex.org/keywords/sensitivity-and-specificity","display_name":"sensitivity and specificity","score":0.8220000267028809},{"id":"https://openalex.org/keywords/point-of-care-testing","display_name":"point-of-care testing","score":0.6809999942779541},{"id":"https://openalex.org/keywords/viral-rna-detection","display_name":"viral RNA detection","score":0.4659999907016754}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.726052463054657},{"id":"https://openalex.org/C160798450","wikidata":"https://www.wikidata.org/wiki/Q4230870","display_name":"Concordance","level":2,"score":0.6324160695075989},{"id":"https://openalex.org/C3007834351","wikidata":"https://www.wikidata.org/wiki/Q82069695","display_name":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)","level":5,"score":0.6056965589523315},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5300262570381165},{"id":"https://openalex.org/C3008058167","wikidata":"https://www.wikidata.org/wiki/Q84263196","display_name":"Coronavirus disease 2019 (COVID-19)","level":4,"score":0.4833318889141083},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.47835296392440796},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.38824060559272766},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37249964475631714},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.31088560819625854},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2599406838417053},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.12215456366539001},{"id":"https://openalex.org/C524204448","wikidata":"https://www.wikidata.org/wiki/Q788926","display_name":"Infectious disease (medical specialty)","level":3,"score":0.12195625901222229},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11407700181007385},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10053154826164246},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.09732615947723389},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000086382","descriptor_name":"COVID-19","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D000086382","descriptor_name":"COVID-19","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000086742","descriptor_name":"COVID-19 Testing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009305","descriptor_name":"Nasopharynx","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D025202","descriptor_name":"Molecular Diagnostic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.3390/v13030420","doi":"https://doi.org/10.3390/v13030420","is_oa":true,"landing_page_url":"https://doi.org/10.3390/v13030420","pdf_url":"https://www.mdpi.com/1999-4915/13/3/420/pdf?version=1615171161","source":{"id":"https://openalex.org/S55776271","display_name":"Viruses","issn_l":"1999-4915","issn":["1999-4915"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Viruses","raw_type":"journal-article"},{"id":"pmid:33807908","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33807908","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Viruses","raw_type":"Evaluation Study"},{"id":"pmh:oai:pubmedcentral.nih.gov:7998296","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC7998296/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Viruses","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:953bfcaf716e4741964b7a99cd87ad6e","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/953bfcaf716e4741964b7a99cd87ad6e","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Viruses, Vol 13, Iss 3, p 420 (2021)","raw_type":"article"},{"id":"pmh:oai:localhost:20.500.12123/9128","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/20.500.12123/9128","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Viruses 13 (3) : 420 (Marzo 2021)","raw_type":"http://purl.org/coar/resource_type/c_6501"},{"id":"pmh:oai:ri.conicet.gov.ar:11336/135603","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11336/135603","pdf_url":null,"source":{"id":"https://openalex.org/S4306402455","display_name":"CONICET Digital (CONICET)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210148385","host_organization_name":"Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society","host_organization_lineage":["https://openalex.org/I4210148385"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.3390/v13030420","doi":"https://doi.org/10.3390/v13030420","is_oa":true,"landing_page_url":"https://doi.org/10.3390/v13030420","pdf_url":"https://www.mdpi.com/1999-4915/13/3/420/pdf?version=1615171161","source":{"id":"https://openalex.org/S55776271","display_name":"Viruses","issn_l":"1999-4915","issn":["1999-4915"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Viruses","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8199999928474426}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3135581941.pdf","grobid_xml":"https://content.openalex.org/works/W3135581941.grobid-xml"},"referenced_works_count":13,"referenced_works":["https://openalex.org/W2606915822","https://openalex.org/W2800905921","https://openalex.org/W3008349875","https://openalex.org/W3009906937","https://openalex.org/W3015040045","https://openalex.org/W3017334101","https://openalex.org/W3017669300","https://openalex.org/W3024194273","https://openalex.org/W3026715285","https://openalex.org/W3034747739","https://openalex.org/W3046937816","https://openalex.org/W3084605929","https://openalex.org/W3087541931"],"related_works":["https://openalex.org/W4206266534","https://openalex.org/W4316499309","https://openalex.org/W3080514314","https://openalex.org/W4407789558","https://openalex.org/W3047013814","https://openalex.org/W3109667847","https://openalex.org/W3173191945","https://openalex.org/W4310465776","https://openalex.org/W4390876454","https://openalex.org/W3026715285"],"abstract_inverted_index":{"We":[0,169],"evaluated":[1],"a":[2,22,126,141],"lyophilized":[3],"CRISPR-Cas12":[4,19],"assay":[5],"for":[6,139,214],"SARS-CoV-2":[7,10,37,101,135,177],"detection":[8],"(Lyo-CRISPR":[9],"kit)":[11],"based":[12],"on":[13,89],"reverse":[14],"transcription,":[15],"isothermal":[16],"amplification,":[17],"and":[18,49,111,151,192,198,216],"reaction.":[20],"From":[21],"total":[23],"of":[24,144,153,174,183,206],"210":[25],"RNA":[26],"samples":[27,48,56,92,110,146],"extracted":[28],"from":[29],"nasopharyngeal":[30,91],"swabs":[31],"using":[32,61],"spin":[33],"columns,":[34],"the":[35,78,99,130,164,172,175,187,211],"Lyo-CRISPR":[36,100,134,176],"kit":[38,102,136,178,208],"detected":[39,103],"105/105":[40],"(100%;":[41,113],"95%":[42,52,106,114],"confidence":[43],"interval":[44],"(CI):":[45],"96.55-100)":[46],"positive":[47,109],"104/105":[50],"(99.05%;":[51],"CI:":[53,107,115],"94.81-99.97)":[54],"negative":[55,117],"that":[57,171,182],"were":[58],"previously":[59],"tested":[60],"commercial":[62,184],"RT-qPCR.":[63],"The":[64,119,133,204],"estimated":[65,120],"overall":[66],"Kappa":[67,121],"index":[68,122],"was":[69,86,123,137,161,179,189],"0.991,":[70],"reflecting":[71],"an":[72],"almost":[73],"perfect":[74],"concordance":[75,128],"level":[76],"between":[77,129],"two":[79],"diagnostic":[80],"tests.":[81],"An":[82],"initial":[83],"validation":[84],"test":[85,131],"also":[87],"performed":[88],"30":[90],"collected":[93],"in":[94,97,220],"lysis":[95],"buffer,":[96],"which":[98],"20/21":[104],"(95.24%;":[105],"76.18-99.88)":[108],"9/9":[112],"66.37-100)":[116],"samples.":[118],"0.923,":[124],"indicating":[125],"strong":[127],"procedures.":[132],"suitable":[138],"detecting":[140],"wide":[142],"range":[143],"RT-qPCR-positive":[145],"(cycle":[147],"threshold":[148],"range:":[149],"11.45-36.90)":[150],"dilutions":[152],"heat-inactivated":[154],"virus":[155],"(range:":[156],"2.5-100":[157],"copies/\u00b5L);":[158],"no":[159],"cross-reaction":[160],"observed":[162],"with":[163],"other":[165],"respiratory":[166],"pathogens":[167],"tested.":[168],"demonstrated":[170],"performance":[173],"similar":[180],"to":[181],"RT-qPCR,":[185],"as":[186],"former":[188],"highly":[190],"sensitive":[191],"specific,":[193],"timesaving":[194],"(1.5":[195],"h),":[196],"inexpensive,":[197],"did":[199],"not":[200],"require":[201],"sophisticated":[202],"equipment.":[203],"use":[205],"this":[207],"would":[209],"reduce":[210],"time":[212],"taken":[213],"diagnosis":[215,219],"facilitate":[217],"molecular":[218],"low-resource":[221],"laboratories.":[222]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":15},{"year":2021,"cited_by_count":5}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2021-03-15T00:00:00"}