{"id":"https://openalex.org/W3135572935","doi":"https://doi.org/10.1021/acssensors.1c00284","title":"Quantitative Measurements of Protein Volume and Concentration using Hydrogel-Backed Nanopores","display_name":"Quantitative Measurements of Protein Volume and Concentration using Hydrogel-Backed Nanopores","publication_year":2021,"publication_date":"2021-03-11","ids":{"openalex":"https://openalex.org/W3135572935","doi":"https://doi.org/10.1021/acssensors.1c00284","mag":"3135572935","pmid":"https://pubmed.ncbi.nlm.nih.gov/33703889"},"language":"en","primary_location":{"id":"doi:10.1021/acssensors.1c00284","doi":"https://doi.org/10.1021/acssensors.1c00284","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssensors.1c00284","pdf_url":null,"source":{"id":"https://openalex.org/S2764631046","display_name":"ACS Sensors","issn_l":"2379-3694","issn":["2379-3694"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Sensors","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012614017","display_name":"Reyhaneh Nazarian","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Reyhaneh Nazarian","raw_affiliation_strings":["Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Eric Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eric Lee","raw_affiliation_strings":["Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030164947","display_name":"Brian Siegel","orcid":"https://orcid.org/0000-0001-7654-3472","observed_orcids":["https://orcid.org/0000-0001-7654-3472"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian Siegel","raw_affiliation_strings":["Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069876476","display_name":"Chance Kuo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chance Kuo","raw_affiliation_strings":["Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113582011","display_name":"Shiv J. Acharya","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shiv Acharya","raw_affiliation_strings":["Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063073811","display_name":"Jacob J. Schmidt","orcid":"https://orcid.org/0000-0001-8277-6280","observed_orcids":["https://orcid.org/0000-0001-8277-6280"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jacob Schmidt","raw_affiliation_strings":["Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States"],"raw_orcid":"https://orcid.org/0000-0001-8277-6280","affiliations":[{"raw_affiliation_string":"Department of Bioengineering, UCLA, 420 Westwood Plaza, Los Angeles, California 90095, United States","institution_ids":["https://openalex.org/I161318765"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5063073811"],"corresponding_institution_ids":["https://openalex.org/I161318765"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":0.2276,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.38305393,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"6","issue":"3","first_page":"722","last_page":"726"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11582","display_name":"Nanopore and Nanochannel Transport Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11582","display_name":"Nanopore and Nanochannel Transport Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nanopore-sensing","display_name":"nanopore sensing","score":0.7860000133514404},{"id":"https://openalex.org/keywords/protein-translocation","display_name":"protein translocation","score":0.699999988079071}],"concepts":[{"id":"https://openalex.org/C141795571","wikidata":"https://www.wikidata.org/wiki/Q580942","display_name":"Nanopore","level":2,"score":0.9303028583526611},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.5967888832092285},{"id":"https://openalex.org/C2777516009","wikidata":"https://www.wikidata.org/wiki/Q194207","display_name":"Ethylene glycol","level":2,"score":0.588318943977356},{"id":"https://openalex.org/C20556612","wikidata":"https://www.wikidata.org/wiki/Q4469374","display_name":"Volume (thermodynamics)","level":2,"score":0.5194782018661499},{"id":"https://openalex.org/C2775913793","wikidata":"https://www.wikidata.org/wiki/Q3030793","display_name":"Residence time (fluid dynamics)","level":2,"score":0.4484062194824219},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.42470720410346985},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.41929891705513},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3814328610897064},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.37429898977279663},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3282305598258972},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32013970613479614},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.07205253839492798},{"id":"https://openalex.org/C187320778","wikidata":"https://www.wikidata.org/wiki/Q1349130","display_name":"Geotechnical engineering","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020100","descriptor_name":"Hydrogels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058608","descriptor_name":"Nanopores","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/acssensors.1c00284","doi":"https://doi.org/10.1021/acssensors.1c00284","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssensors.1c00284","pdf_url":null,"source":{"id":"https://openalex.org/S2764631046","display_name":"ACS Sensors","issn_l":"2379-3694","issn":["2379-3694"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Sensors","raw_type":"journal-article"},{"id":"pmid:33703889","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33703889","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS sensors","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W1533734182","https://openalex.org/W1662176830","https://openalex.org/W1803935349","https://openalex.org/W1954798618","https://openalex.org/W1983861942","https://openalex.org/W1997140899","https://openalex.org/W2008843731","https://openalex.org/W2011993186","https://openalex.org/W2024366778","https://openalex.org/W2029311639","https://openalex.org/W2031045367","https://openalex.org/W2035538026","https://openalex.org/W2047721234","https://openalex.org/W2057313370","https://openalex.org/W2059648430","https://openalex.org/W2062025255","https://openalex.org/W2063831714","https://openalex.org/W2070819188","https://openalex.org/W2073463817","https://openalex.org/W2080548491","https://openalex.org/W2083307757","https://openalex.org/W2090924278","https://openalex.org/W2091810350","https://openalex.org/W2092042046","https://openalex.org/W2094153893","https://openalex.org/W2097065797","https://openalex.org/W2116360289","https://openalex.org/W2133372935","https://openalex.org/W2148512205","https://openalex.org/W2151932722","https://openalex.org/W2326335867","https://openalex.org/W2327033711","https://openalex.org/W2342867357","https://openalex.org/W2562252980","https://openalex.org/W2601868508","https://openalex.org/W2755546302","https://openalex.org/W2794127121","https://openalex.org/W2796033973","https://openalex.org/W2796670593","https://openalex.org/W2892547699","https://openalex.org/W2936223241","https://openalex.org/W2944651360","https://openalex.org/W2969758158","https://openalex.org/W3000951288","https://openalex.org/W3021843345"],"related_works":["https://openalex.org/W7010552956","https://openalex.org/W4211180439","https://openalex.org/W2960152696","https://openalex.org/W3000951288","https://openalex.org/W2107926942","https://openalex.org/W2894558132","https://openalex.org/W2442684178","https://openalex.org/W4385295071","https://openalex.org/W2936223241","https://openalex.org/W4403677240"],"abstract_inverted_index":{"Accurate":[0],"identification":[1],"and":[2,29,86,111,158],"quantification":[3],"of":[4,16,20,31,53,68,96,109,122,173],"proteins":[5,78,147],"in":[6,13,84,89],"solution":[7],"using":[8],"nanopores":[9,74],"is":[10],"technically":[11],"challenging":[12],"part":[14],"because":[15],"the":[17,58,63,66,94,123,166,171,174],"large":[18],"fraction":[19],"missed":[21,69],"translocation":[22,98],"events":[23,99],"due":[24],"to":[25,75,137,152],"short":[26],"event":[27],"times":[28],"limitations":[30],"conventional":[32],"current":[33],"amplifiers.":[34],"Previously,":[35],"we":[36,132],"have":[37],"shown":[38],"that":[39,93,112],"a":[40,44,106,139,149,153],"nanopore":[41,154],"interfaced":[42],"with":[43,48,102,126,148],"poly(ethylene":[45],"glycol)-dimethacrylate":[46],"hydrogel":[47],"an":[49,120,134],"average":[50],"mesh":[51],"size":[52,85],"3.1":[54],"nm":[55],"significantly":[56],"enhances":[57],"protein":[59,97,114,140],"residence":[60],"time":[61],"within":[62],"pore,":[64],"reducing":[65],"number":[67],"events.":[70],"We":[71,91],"used":[72],"hydrogel-backed":[73],"sense":[76],"unlabeled":[77],"as":[79,81],"small":[80],"5.5":[82],"kDa":[83],"10":[87],"fM":[88],"concentration.":[90],"show":[92,133],"frequency":[95],"linearly":[100],"scales":[101],"bulk":[103],"concentration":[104],"over":[105],"wide":[107],"range":[108],"concentrations":[110,115],"unknown":[113],"can":[116],"be":[117],"determined":[118],"from":[119,143],"interpolation":[121],"frequency-concentration":[124],"curve":[125],"less":[127],"than":[128],"10%":[129],"error.":[130],"Further,":[131],"iterative":[135],"method":[136],"determine":[138],"volume":[141],"accurately":[142],"measurement":[144],"data":[145],"for":[146,165],"diameter":[150],"comparable":[151],"diameter.":[155],"Our":[156],"measurements":[157],"analysis":[159],"also":[160],"suggest":[161],"several":[162],"competing":[163],"mechanisms":[164],"detection":[167],"enhancement":[168],"enabled":[169],"by":[170],"presence":[172],"hydrogel.":[175]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2021-03-15T00:00:00"}