{"id":"https://openalex.org/W3134965778","doi":"https://doi.org/10.17504/protocols.io.ukgeutw","title":"16S Illumina Primer Plate Resuspension (100 \u00b5M) v1","display_name":"16S Illumina Primer Plate Resuspension (100 \u00b5M) v1","publication_year":2018,"publication_date":"2018-10-12","ids":{"openalex":"https://openalex.org/W3134965778","doi":"https://doi.org/10.17504/protocols.io.ukgeutw","mag":"3134965778"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.ukgeutw","doi":"https://doi.org/10.17504/protocols.io.ukgeutw","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.ukgeutw","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.17504/protocols.io.ukgeutw","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5041534232","display_name":"Rodolfo Antonio Salido Ben\u00edtez","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Rodolfo A Salido Benitez","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5041534232"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.4325999915599823,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.4325999915599823,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.3206000030040741,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.13660000264644623,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/16s-rrna","display_name":"16S rRNA","score":0.8740000128746033},{"id":"https://openalex.org/keywords/v4-region","display_name":"V4 region","score":0.8690000176429749},{"id":"https://openalex.org/keywords/earth-microbiome-project","display_name":"Earth Microbiome Project","score":0.722000002861023},{"id":"https://openalex.org/keywords/microbial-community-sequencing","display_name":"microbial community sequencing","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7901513576507568},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.4856206476688385},{"id":"https://openalex.org/C143121216","wikidata":"https://www.wikidata.org/wiki/Q1330402","display_name":"Microbiome","level":2,"score":0.462027370929718},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4053381681442261},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.36011844873428345},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34934741258621216},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.3417341709136963},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.3307977318763733},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.2790312170982361},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2604386806488037},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.11582368612289429},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.06637701392173767},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.ukgeutw","doi":"https://doi.org/10.17504/protocols.io.ukgeutw","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.ukgeutw","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.ukgeutw","doi":"https://doi.org/10.17504/protocols.io.ukgeutw","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.ukgeutw","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3133825341","https://openalex.org/W7160067194","https://openalex.org/W3133680828","https://openalex.org/W3013903774","https://openalex.org/W3134232876","https://openalex.org/W4320508620","https://openalex.org/W4320508584","https://openalex.org/W7097081922","https://openalex.org/W34716119","https://openalex.org/W4411269782"],"abstract_inverted_index":{"The":[0],"following":[1],"protocol":[2],"describes":[3],"resuspension":[4],"of":[5],"lyophilized":[6],"primers":[7],"from":[8,13],"(4)":[9],"96-Well":[10],"plates":[11],"shipped":[12],"Integrated":[14],"DNA":[15],"Technologies":[16],"(IDT)":[17],"at":[18],"3nmol":[19],"per":[20],"well":[21],"using":[22],"the":[23,31,47],"epMotion":[24],"5075.":[25],"For":[26],"information":[27],"about":[28],"ordering":[29],"consult":[30],"Earth":[32,48],"Micriobiome":[33],"Projects":[34],"website.":[35,51],"Barcoded":[36],"primer":[37],"constructs":[38],"for":[39],"16S":[40],"V4":[41],"region":[42],"are":[43],"also":[44],"described":[45],"in":[46],"Microbiome":[49],"Project":[50]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2021-03-15T00:00:00"}