{"id":"https://openalex.org/W3133886118","doi":"https://doi.org/10.1002/2211-5463.13139","title":"CRISPR\u2010based knock\u2010in mutagenesis of the pioneer transcription factor FOXA1: optimization of strategies for multi\u2010allelic proteins in cancer cells","display_name":"CRISPR\u2010based knock\u2010in mutagenesis of the pioneer transcription factor FOXA1: optimization of strategies for multi\u2010allelic proteins in cancer cells","publication_year":2021,"publication_date":"2021-03-05","ids":{"openalex":"https://openalex.org/W3133886118","doi":"https://doi.org/10.1002/2211-5463.13139","mag":"3133886118","pmid":"https://pubmed.ncbi.nlm.nih.gov/33666335","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8167868"},"language":"en","primary_location":{"id":"doi:10.1002/2211-5463.13139","doi":"https://doi.org/10.1002/2211-5463.13139","is_oa":true,"landing_page_url":"https://doi.org/10.1002/2211-5463.13139","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/2211-5463.13139","source":{"id":"https://openalex.org/S2898225119","display_name":"FEBS Open Bio","issn_l":"2211-5463","issn":["2211-5463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Open Bio","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/2211-5463.13139","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100768720","display_name":"Li Shen","orcid":"https://orcid.org/0000-0003-2947-3787","observed_orcids":["https://orcid.org/0000-0003-2947-3787"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shen Li","raw_affiliation_strings":["The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084260063","display_name":"Joseph P. Garay","orcid":"https://orcid.org/0000-0002-2287-3051","observed_orcids":["https://orcid.org/0000-0002-2287-3051"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph P. Garay","raw_affiliation_strings":["The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027880050","display_name":"Colby A. Tubbs","orcid":"https://orcid.org/0000-0001-7676-8735","observed_orcids":["https://orcid.org/0000-0001-7676-8735"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Colby A. Tubbs","raw_affiliation_strings":["The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5033307249","display_name":"Hector L. Franco","orcid":"https://orcid.org/0000-0001-9354-0679","observed_orcids":["https://orcid.org/0000-0001-9354-0679"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hector L. Franco","raw_affiliation_strings":["The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA"],"raw_orcid":"https://orcid.org/0000-0001-9354-0679","affiliations":[{"raw_affiliation_string":"The Lineberger Comprehensive Cancer Center, Department of Genetics, University of North Carolina at Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5033307249"],"corresponding_institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"],"apc_list":{"value":1850,"currency":"USD","value_usd":1850},"apc_paid":{"value":1850,"currency":"USD","value_usd":1850},"fwci":0.3576,"has_fulltext":true,"cited_by_count":6,"citation_normalized_percentile":{"value":0.46187902,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"11","issue":"6","first_page":"1537","last_page":"1551"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/foxa1","display_name":"FOXA1","score":0.9900000095367432},{"id":"https://openalex.org/keywords/pioneer-transcription-factors","display_name":"pioneer transcription factors","score":0.8970000147819519},{"id":"https://openalex.org/keywords/allele-frequency-estimation","display_name":"allele frequency estimation","score":0.9539999961853027},{"id":"https://openalex.org/keywords/mcf-7-cells","display_name":"MCF-7 cells","score":0.8859999775886536},{"id":"https://openalex.org/keywords/estrogen-receptor-signaling","display_name":"estrogen receptor signaling","score":0.7680000066757202},{"id":"https://openalex.org/keywords/androgen-receptor-signaling","display_name":"androgen receptor signaling","score":0.9940000176429749},{"id":"https://openalex.org/keywords/allele-specific-pcr","display_name":"allele-specific PCR","score":0.9419999718666077}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8258999586105347},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6798783540725708},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.6497136354446411},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.4859658479690552},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4779796004295349},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.47797057032585144},{"id":"https://openalex.org/C134486438","wikidata":"https://www.wikidata.org/wiki/Q3123214","display_name":"Forward genetics","level":4,"score":0.4716797471046448},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4698542356491089},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.44322407245635986},{"id":"https://openalex.org/C154456304","wikidata":"https://www.wikidata.org/wiki/Q18027046","display_name":"FOXA1","level":4,"score":0.41661933064460754},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.4118230938911438},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.41138356924057007},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.3640521764755249}],"mesh":[{"descriptor_ui":"D000076987","descriptor_name":"CRISPR-Associated Protein 9","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051541","descriptor_name":"Hepatocyte Nuclear Factor 3-alpha","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1002/2211-5463.13139","doi":"https://doi.org/10.1002/2211-5463.13139","is_oa":true,"landing_page_url":"https://doi.org/10.1002/2211-5463.13139","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/2211-5463.13139","source":{"id":"https://openalex.org/S2898225119","display_name":"FEBS Open Bio","issn_l":"2211-5463","issn":["2211-5463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Open Bio","raw_type":"journal-article"},{"id":"doi:10.1101/2020.10.27.356824","doi":"https://doi.org/10.1101/2020.10.27.356824","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2020.10.27.356824","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2020/10/27/2020.10.27.356824.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:8167868","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8167868","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Open Bio","raw_type":"Text"},{"id":"pmid:33666335","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33666335","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS open bio","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:ba9a01acea6c4776a594080b4e3dd4f2","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/ba9a01acea6c4776a594080b4e3dd4f2","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"FEBS Open Bio, Vol 11, Iss 6, Pp 1537-1551 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1002/2211-5463.13139","doi":"https://doi.org/10.1002/2211-5463.13139","is_oa":true,"landing_page_url":"https://doi.org/10.1002/2211-5463.13139","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/2211-5463.13139","source":{"id":"https://openalex.org/S2898225119","display_name":"FEBS Open Bio","issn_l":"2211-5463","issn":["2211-5463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Open Bio","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.7246000170707703}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2579845599","display_name":"Mechanisms of FoxA1 Latent Enhancer Formation in Response to Proinflammatory Signaling in Hormone Dependent Cancers","funder_award_id":"R00CA204628","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6694186295","display_name":null,"funder_award_id":"R00\u2010CA204628\u201002","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G866894718","display_name":"Mechanisms of FoxA1 Latent Enhancer Formation in Response to Proinflammatory Signaling in Hormone Dependent Cancers","funder_award_id":"K99CA204628","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000890931","display_name":"SPORE in Breast Cancer","funder_award_id":"P50CA058223","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3133886118.pdf","grobid_xml":"https://content.openalex.org/works/W3133886118.grobid-xml"},"referenced_works_count":44,"referenced_works":["https://openalex.org/W1482231686","https://openalex.org/W1589220373","https://openalex.org/W1906155566","https://openalex.org/W1968342623","https://openalex.org/W1977531433","https://openalex.org/W1977709885","https://openalex.org/W1986718765","https://openalex.org/W1989568784","https://openalex.org/W1991378095","https://openalex.org/W2007353945","https://openalex.org/W2008147758","https://openalex.org/W2010986636","https://openalex.org/W2029994182","https://openalex.org/W2041797639","https://openalex.org/W2043398720","https://openalex.org/W2045435533","https://openalex.org/W2046389285","https://openalex.org/W2047032063","https://openalex.org/W2063148079","https://openalex.org/W2064036807","https://openalex.org/W2088816616","https://openalex.org/W2091615587","https://openalex.org/W2110444739","https://openalex.org/W2118746294","https://openalex.org/W2122097157","https://openalex.org/W2162536970","https://openalex.org/W2164934988","https://openalex.org/W2168245648","https://openalex.org/W2336105423","https://openalex.org/W2461013690","https://openalex.org/W2530601336","https://openalex.org/W2538138106","https://openalex.org/W2547418311","https://openalex.org/W2614952490","https://openalex.org/W2748595859","https://openalex.org/W2759723095","https://openalex.org/W2768415485","https://openalex.org/W2795227812","https://openalex.org/W2804566227","https://openalex.org/W2913794820","https://openalex.org/W2914038249","https://openalex.org/W2921058482","https://openalex.org/W2981137429","https://openalex.org/W2985493807"],"related_works":["https://openalex.org/W3164842357","https://openalex.org/W2555902114","https://openalex.org/W3173962112","https://openalex.org/W3045751052","https://openalex.org/W2997897565","https://openalex.org/W2774641236","https://openalex.org/W3141703053","https://openalex.org/W2788594080","https://openalex.org/W4243745812","https://openalex.org/W2977452756"],"abstract_inverted_index":{"Precise":[0],"genome":[1,167],"engineering":[2],"of":[3,12,20,43,70,83,89,93,104,108,119,163,169,182,244,253,272,297],"living":[4],"cells":[5,98,100,147],"has":[6,31,39],"been":[7],"revolutionized":[8],"by":[9,222,233],"the":[10,13,21,41,79,86,129,174,251,294,302],"introduction":[11],"highly":[14],"specific":[15,58,170],"and":[16,38,116,124,148,155,184,199,207],"easily":[17,220],"programmable":[18],"properties":[19],"clustered":[22],"regularly":[23],"interspaced":[24],"short":[25],"palindromic":[26],"repeats":[27],"(CRISPR)":[28],"technology.":[29],"This":[30],"greatly":[32],"accelerated":[33],"research":[34],"into":[35],"human":[36],"health":[37],"facilitated":[40],"discovery":[42],"novel":[44],"therapeutics.":[45],"CRISPR-Cas9":[46],"is":[47,121,219],"most":[48,261],"widely":[49],"employed":[50],"for":[51,136,283],"its":[52,298],"ability":[53],"to":[54,65,76,292],"inactivate":[55],"or":[56],"knockout":[57],"genes,":[59],"but":[60],"can":[61,74],"be":[62],"also":[63],"used":[64],"introduce":[66],"subtle":[67],"site-specific":[68],"substitutions":[69,203],"DNA":[71],"sequences":[72],"that":[73,111,214,263],"lead":[75],"changes":[77],"in":[78,95,128,144,188,246],"amino":[80,171,201],"acid":[81,202],"composition":[82],"proteins.":[84],"Despite":[85],"proven":[87],"success":[88],"CRISPR-based":[90],"knock-in":[91,142,290],"strategies":[92],"genes":[94],"typical":[96],"diploid":[97],"(i.e.,":[99],"containing":[101],"two":[102],"sets":[103],"chromosomes),":[105],"precise":[106,289],"editing":[107,168,252],"cancer":[109,146,191,248],"cells,":[110,249],"typically":[112],"have":[113],"unstable":[114],"genomes":[115],"multiple":[117],"copies":[118,242],"chromosomes,":[120],"more":[122],"challenging":[123],"not":[125],"adequately":[126],"addressed":[127],"literature.":[130],"Herein,":[131],"we":[132,165,212,230,278],"detail":[133],"our":[134,150],"methodology":[135,282],"replacing":[137,284],"endogenous":[138,285],"proteins":[139],"with":[140,226,288],"intended":[141,200],"mutants":[143,267,291],"polyploid":[145],"discuss":[149],"experimental":[151],"design,":[152],"screening":[153],"strategy,":[154],"facile":[156],"allele":[157,216],"frequency":[158,217],"estimation":[159,218],"methodology.":[160],"As":[161,258],"proof":[162],"principle,":[164],"performed":[166],"acids":[172],"within":[173,301],"pioneer":[175],"transcription":[176],"factor":[177],"FOXA1,":[178],"a":[179,259,275],"critical":[180],"component":[181],"estrogen":[183],"androgen":[185],"receptor":[186],"signaling,":[187],"MCF-7":[189],"breast":[190,247],"cells.":[192],"We":[193],"confirm":[194],"mutant":[195,215],"FOXA1":[196,245,266,273,287],"protein":[197,255],"expression":[198],"via":[204],"western":[205],"blotting":[206],"mass":[208],"spectrometry.":[209],"In":[210],"addition,":[211],"show":[213],"achieved":[221],"topoisomerase-based":[223],"cloning":[224],"combined":[225],"allele-specific":[227],"PCR,":[228],"which":[229],"later":[231],"confirmed":[232],"next-generation":[234],"RNA-sequencing.":[235],"Typically,":[236],"there":[237],"are":[238],"4":[239],"-":[240],"5":[241],"(alleles)":[243],"making":[250],"this":[254],"inherently":[256],"challenging.":[257],"result,":[260],"studies":[262],"focus":[264],"on":[265,269],"rely":[268],"ectopic":[270],"overexpression":[271],"from":[274],"plasmid.":[276],"Therefore,":[277],"provide":[279],"an":[280],"optimized":[281],"wild-type":[286],"enable":[293],"systematic":[295],"analysis":[296],"molecular":[299],"mechanisms":[300],"appropriate":[303],"physiological":[304],"context.":[305]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":4}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2021-03-15T00:00:00"}
