{"id":"https://openalex.org/W3133764520","doi":"https://doi.org/10.22058/jpmb.2020.114833.1191","title":"A Comparative Analysis of the Hairy Root Induction Methods in Hypericum perforatum","display_name":"A Comparative Analysis of the Hairy Root Induction Methods in Hypericum perforatum","publication_year":2019,"publication_date":"2019-06-01","ids":{"openalex":"https://openalex.org/W3133764520","doi":"https://doi.org/10.22058/jpmb.2020.114833.1191","mag":"3133764520"},"language":"en","primary_location":{"id":"mag:3133764520","doi":"https://doi.org/10.22058/jpmb.2020.114833.1191","is_oa":false,"landing_page_url":"http://www.jpmb-gabit.ir/article_39120_6cfefbb038553f765e70859782f57751.pdf","pdf_url":null,"source":{"id":"https://openalex.org/S4306515970","display_name":"Journal of plant molecular breeding","issn_l":"2322-3332","issn":["2322-3332","2322-5092"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310319088","host_organization_name":"Genetics and Agricultural Biotechnology Institute of Tabarestan","host_organization_lineage":["https://openalex.org/P4310319088"],"host_organization_lineage_names":["Genetics and Agricultural Biotechnology Institute of Tabarestan"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Journal of plant molecular breeding","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090061934","display_name":"Mahsa Montazeri","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I184896493","display_name":"Sari Agricultural Sciences and Natural Resources University","ror":"https://ror.org/0284vkq26","country_code":"IR","type":"education","lineage":["https://openalex.org/I184896493"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Mahsa Montazeri","raw_affiliation_strings":["Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran","institution_ids":["https://openalex.org/I184896493"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033336695","display_name":"Ali Pakdin\u2010Parizi","orcid":"https://orcid.org/0000-0003-4570-8021","observed_orcids":["https://orcid.org/0000-0003-4570-8021"]},"institutions":[{"id":"https://openalex.org/I184896493","display_name":"Sari Agricultural Sciences and Natural Resources University","ror":"https://ror.org/0284vkq26","country_code":"IR","type":"education","lineage":["https://openalex.org/I184896493"]},{"id":"https://openalex.org/I4871159","display_name":"University of Mazandaran","ror":"https://ror.org/05fp9g671","country_code":"IR","type":"education","lineage":["https://openalex.org/I4871159"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Ali Pakdin Parizi","raw_affiliation_strings":["Genetics and Agricultural Biotechnology Institute of Tabarestan Sari Agricultural Sciences and Natural Resources University Mazandaran Iran"],"raw_orcid":"https://orcid.org/0000-0003-4570-8021","affiliations":[{"raw_affiliation_string":"Genetics and Agricultural Biotechnology Institute of Tabarestan Sari Agricultural Sciences and Natural Resources University Mazandaran Iran","institution_ids":["https://openalex.org/I184896493","https://openalex.org/I4871159"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008055191","display_name":"Hamid Najafi-Zarrini","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I184896493","display_name":"Sari Agricultural Sciences and Natural Resources University","ror":"https://ror.org/0284vkq26","country_code":"IR","type":"education","lineage":["https://openalex.org/I184896493"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Hamid Najafi-Zarrini","raw_affiliation_strings":["Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran","institution_ids":["https://openalex.org/I184896493"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108107468","display_name":"Mohammad Azadbakht","orcid":"https://orcid.org/0000-0002-6298-8718","observed_orcids":["https://orcid.org/0000-0002-6298-8718"]},"institutions":[{"id":"https://openalex.org/I88085062","display_name":"Mazandaran University of Medical Sciences","ror":"https://ror.org/02wkcrp04","country_code":"IR","type":"education","lineage":["https://openalex.org/I88085062"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Mohammad Azadbakht","raw_affiliation_strings":["Department of Pharmacognosy, Faculty of Pharmacy Mazandaran University of Medical Sciences, Sari. Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacognosy, Faculty of Pharmacy Mazandaran University of Medical Sciences, Sari. Iran","institution_ids":["https://openalex.org/I88085062"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068585844","display_name":"Ghorbanali Nematzadeh","orcid":"https://orcid.org/0000-0002-1371-0293","observed_orcids":["https://orcid.org/0000-0002-1371-0293"]},"institutions":[{"id":"https://openalex.org/I184896493","display_name":"Sari Agricultural Sciences and Natural Resources University","ror":"https://ror.org/0284vkq26","country_code":"IR","type":"education","lineage":["https://openalex.org/I184896493"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Ghorbanali Nematzadeh","raw_affiliation_strings":["Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran"],"raw_orcid":"https://orcid.org/0000-0002-1371-0293","affiliations":[{"raw_affiliation_string":"Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran","institution_ids":["https://openalex.org/I184896493"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004212022","display_name":"Zahra Gholami","orcid":"https://orcid.org/0000-0003-1762-2626","observed_orcids":["https://orcid.org/0000-0003-1762-2626"]},"institutions":[{"id":"https://openalex.org/I184896493","display_name":"Sari Agricultural Sciences and Natural Resources University","ror":"https://ror.org/0284vkq26","country_code":"IR","type":"education","lineage":["https://openalex.org/I184896493"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Zahra Gholami","raw_affiliation_strings":["Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran"],"raw_orcid":"https://orcid.org/0000-0003-1762-2626","affiliations":[{"raw_affiliation_string":"Department of plant breeding and biotechnology Faculty of crop sciences Sari Agricultural Sciences and Natural Resources University Sari Iran","institution_ids":["https://openalex.org/I184896493"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1818,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.49267363,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"7","issue":"1","first_page":"67","last_page":"76"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11578","display_name":"Plant Pathogenic Bacteria Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hypericum-perforatum","display_name":"Hypericum perforatum","score":0.9879999756813049},{"id":"https://openalex.org/keywords/hairy-root-induction","display_name":"hairy root induction","score":0.9520000219345093},{"id":"https://openalex.org/keywords/agrobacterium-rhizogenes","display_name":"Agrobacterium rhizogenes","score":0.9959999918937683},{"id":"https://openalex.org/keywords/acetosyringone","display_name":"acetosyringone","score":0.984000027179718},{"id":"https://openalex.org/keywords/explant-type","display_name":"explant type","score":0.6050000190734863},{"id":"https://openalex.org/keywords/transgenic-hairy-roots","display_name":"transgenic hairy roots","score":0.8619999885559082},{"id":"https://openalex.org/keywords/secondary-metabolites","display_name":"secondary metabolites","score":0.6949999928474426},{"id":"https://openalex.org/keywords/hyperforin","display_name":"hyperforin","score":0.6520000100135803},{"id":"https://openalex.org/keywords/hypericin","display_name":"hypericin","score":0.9739999771118164}],"concepts":[{"id":"https://openalex.org/C2776651039","wikidata":"https://www.wikidata.org/wiki/Q158289","display_name":"Hypericum perforatum","level":2,"score":0.9616568088531494},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.7680110931396484},{"id":"https://openalex.org/C2778432610","wikidata":"https://www.wikidata.org/wiki/Q425409","display_name":"Hypericin","level":2,"score":0.6834691762924194},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.6159840822219849},{"id":"https://openalex.org/C157428477","wikidata":"https://www.wikidata.org/wiki/Q5639642","display_name":"Hairy root culture","level":5,"score":0.6000533103942871},{"id":"https://openalex.org/C2778350159","wikidata":"https://www.wikidata.org/wiki/Q412742","display_name":"Hyperforin","level":3,"score":0.5778241157531738},{"id":"https://openalex.org/C180032290","wikidata":"https://www.wikidata.org/wiki/Q1664296","display_name":"Inoculation","level":2,"score":0.48749732971191406},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.4662415385246277},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.459235280752182},{"id":"https://openalex.org/C2779012821","wikidata":"https://www.wikidata.org/wiki/Q1329233","display_name":"Elicitor","level":3,"score":0.4569126069545746},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.43619513511657715},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.1957833468914032},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.07601034641265869},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0753941535949707},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:3133764520","doi":"https://doi.org/10.22058/jpmb.2020.114833.1191","is_oa":false,"landing_page_url":"http://www.jpmb-gabit.ir/article_39120_6cfefbb038553f765e70859782f57751.pdf","pdf_url":null,"source":{"id":"https://openalex.org/S4306515970","display_name":"Journal of plant molecular breeding","issn_l":"2322-3332","issn":["2322-3332","2322-5092"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310319088","host_organization_name":"Genetics and Agricultural Biotechnology Institute of Tabarestan","host_organization_lineage":["https://openalex.org/P4310319088"],"host_organization_lineage_names":["Genetics and Agricultural Biotechnology Institute of Tabarestan"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Journal of plant molecular breeding","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.6735000014305115,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W33640325","https://openalex.org/W1515209246","https://openalex.org/W1580875586","https://openalex.org/W1653195719","https://openalex.org/W1861134384","https://openalex.org/W1904551732","https://openalex.org/W1965555893","https://openalex.org/W1971361655","https://openalex.org/W1971981891","https://openalex.org/W1978871608","https://openalex.org/W1983229096","https://openalex.org/W1996458161","https://openalex.org/W2001149801","https://openalex.org/W2002814625","https://openalex.org/W2015271170","https://openalex.org/W2023870314","https://openalex.org/W2024556021","https://openalex.org/W2024615751","https://openalex.org/W2026169487","https://openalex.org/W2040899136","https://openalex.org/W2042001975","https://openalex.org/W2050894006","https://openalex.org/W2052813349","https://openalex.org/W2058413317","https://openalex.org/W2067304399","https://openalex.org/W2068303746","https://openalex.org/W2077545872","https://openalex.org/W2086645111","https://openalex.org/W2087224775","https://openalex.org/W2092942428","https://openalex.org/W2117382750","https://openalex.org/W2127191326","https://openalex.org/W2140755103","https://openalex.org/W2147431852","https://openalex.org/W2470916884","https://openalex.org/W2473418640","https://openalex.org/W3124875492","https://openalex.org/W4236636802"],"related_works":["https://openalex.org/W2147431852","https://openalex.org/W7000697829","https://openalex.org/W2001149801","https://openalex.org/W3095023829","https://openalex.org/W2341161661","https://openalex.org/W2354057110","https://openalex.org/W1905445222","https://openalex.org/W2018333228","https://openalex.org/W2742156521","https://openalex.org/W2896494645"],"abstract_inverted_index":{"Hypericum":[0],"perforatum":[1,60,163],"is":[2,26,152],"a":[3],"medicinal":[4],"plant":[5,25,71],"which":[6],"Hypericin,":[7],"Hyperforin":[8],"and":[9,28,43,82,84,89,94,134,137],"phenolic":[10],"compounds":[11],"are":[12],"its":[13],"active":[14],"secondary":[15],"metabolites.":[16],"Hairy":[17],"root":[18,62,118,165,184],"induction":[19,63,119,185],"by":[20],"Agrobacterium":[21],"rhizogenes":[22,48,66],"in":[23,40,158,161,186],"this":[24,53],"difficult":[27],"has":[29,49],"low":[30],"efficiency.":[31],"In":[32,167],"the":[33,55,113,126,145,169,177],"present":[34],"study":[35],"two":[36],"inoculation":[37],"methods,":[38],"immersion":[39,157],"bacterial":[41,159],"suspension":[42,160],"direct":[44,149,173],"injection":[45,150,174],"of":[46,120,125,147,179],"A.":[47,65],"been":[50],"compared.":[51],"For":[52],"purpose,":[54],"best":[56,114],"conditions":[57,115],"for":[58,96,116],"H.":[59,121,162,187],"hairy":[61,117,128,164,183],"including":[64],"strains":[67],"(A4,":[68],"LBA9402,":[69],"NCPPB2656),":[70],"explants":[72],"(Stem,":[73],"Apical":[74],"bud,":[75],"leaves),":[76],"co-cultivation":[77,108],"media":[78],"(MS,":[79],"\u00bdMS,":[80],"B5,":[81],"\u00bdB5)":[83],"Acetosyringone":[85],"(AS)":[86],"concentration":[87],"(0":[88],"100":[90],"\u00b5M)":[91],"were":[92],"specified":[93],"used":[95],"comparative":[97],"analysis.":[98],"It":[99],"was":[100,130],"found":[101],"that":[102,144,172],"strain":[103],"A4,":[104],"Stem":[105],"explants,":[106],"\u00bdMS":[107],"medium":[109],"without":[110],"AS":[111],"constitute":[112],"perforatum.":[122,188],"Transgenic":[123],"nature":[124],"potential":[127],"roots":[129],"confirmed":[131],"using":[132],"PCR":[133],"specific":[135],"rolB":[136],"rolC":[138],"genes":[139],"primers.":[140],"The":[141],"results":[142,170],"showed":[143],"efficiency":[146],"applying":[148],"method":[151,178],"four":[153],"times":[154],"higher":[155],"than":[156],"induction.":[166],"general,":[168],"indicate":[171],"can":[175],"be":[176],"choice":[180],"to":[181],"successful":[182]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-03-15T00:00:00"}