{"id":"https://openalex.org/W3133597113","doi":null,"title":"Cyclic AMP-dependent phosphorylation of voltage-sensitive sodium channels in primary cultures of rat brain cells","display_name":"Cyclic AMP-dependent phosphorylation of voltage-sensitive sodium channels in primary cultures of rat brain cells","publication_year":1986,"publication_date":"1986-03-05","ids":{"openalex":"https://openalex.org/W3133597113","mag":"3133597113"},"language":"en","primary_location":{"id":"mag:3133597113","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/6943038-cyclic-amp-dependent-phosphorylation-voltage-sensitive-sodium-channels-primary-cultures-rat-brain-cells","pdf_url":null,"source":{"id":"https://openalex.org/S4306510388","display_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029095556","display_name":"Sandra S. Rossie","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sandra Rossie","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5078941732","display_name":"William A. Catterall","orcid":"https://orcid.org/0000-0001-5509-3580","observed_orcids":["https://orcid.org/0000-0001-5509-3580"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W A Catterall","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.28064974,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/voltage-gated-sodium-channels","display_name":"voltage-gated sodium channels","score":0.9380000233650208},{"id":"https://openalex.org/keywords/camp-dependent-phosphorylation","display_name":"cAMP-dependent phosphorylation","score":0.8450000286102295},{"id":"https://openalex.org/keywords/protein-kinase-a","display_name":"protein kinase A","score":0.9710000157356262},{"id":"https://openalex.org/keywords/alpha-subunit","display_name":"alpha subunit","score":0.800000011920929},{"id":"https://openalex.org/keywords/forskolin","display_name":"forskolin","score":0.7200000286102295},{"id":"https://openalex.org/keywords/8-bromo-camp","display_name":"8-bromo-cAMP","score":0.7670000195503235},{"id":"https://openalex.org/keywords/3-isobutyl-1-methylxanthine","display_name":"3-isobutyl-1-methylxanthine","score":0.9950000047683716},{"id":"https://openalex.org/keywords/intracellular-camp","display_name":"intracellular cAMP","score":0.9139999747276306},{"id":"https://openalex.org/keywords/brain-neurons","display_name":"brain neurons","score":0.7310000061988831},{"id":"https://openalex.org/keywords/primary-neuronal-cultures","display_name":"primary neuronal cultures","score":0.5889999866485596},{"id":"https://openalex.org/keywords/synaptosomes","display_name":"synaptosomes","score":0.5630000233650208}],"concepts":[{"id":"https://openalex.org/C2779276759","wikidata":"https://www.wikidata.org/wiki/Q412747","display_name":"Forskolin","level":3,"score":0.8035600781440735},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.7970839738845825},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.6708414554595947},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.5783237218856812},{"id":"https://openalex.org/C50952357","wikidata":"https://www.wikidata.org/wiki/Q424960","display_name":"Sodium channel","level":3,"score":0.5320302844047546},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5206305384635925},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.491974800825119},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.44739946722984314},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4287390112876892},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4241853356361389},{"id":"https://openalex.org/C195794163","wikidata":"https://www.wikidata.org/wiki/Q420877","display_name":"Protein kinase C","level":3,"score":0.41840147972106934},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.4152190685272217},{"id":"https://openalex.org/C87325107","wikidata":"https://www.wikidata.org/wiki/Q7251493","display_name":"Protein phosphorylation","level":4,"score":0.413756787776947},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.40305620431900024},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.3958350419998169},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.3676353693008423},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.36708658933639526},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.30662497878074646},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.18050289154052734},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:3133597113","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/6943038-cyclic-amp-dependent-phosphorylation-voltage-sensitive-sodium-channels-primary-cultures-rat-brain-cells","pdf_url":null,"source":{"id":"https://openalex.org/S4306510388","display_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1492344127","https://openalex.org/W1983396112","https://openalex.org/W1555849435","https://openalex.org/W1990133698","https://openalex.org/W1630525523","https://openalex.org/W1596672035","https://openalex.org/W1487738788","https://openalex.org/W2072514225","https://openalex.org/W1509592135","https://openalex.org/W1538429608"],"abstract_inverted_index":{"The":[0,24,100,136],"..cap":[1],"alpha..":[2],"subunit":[3,78],"of":[4,32,79,89,97,102,114,118,124,132,138,167,180,183,198],"the":[5,76,112,178],"voltage-sensitive":[6,184],"Na":[7,33,67,98,125,143,172,185],"channel":[8,107],"from":[9],"rat":[10],"brain":[11,39,189],"is":[12,108,191],"phosphorylated":[13],"by":[14,71,193],"cAMP-dependent":[15,30,80,94,181],"protein":[16,81],"kinase":[17,82],"in":[18,22,35,48,187,195],"purified":[19],"preparations":[20],"and":[21,46,66,83,152,160],"synaptosomes.":[23],"authors":[25],"have":[26],"begun":[27],"to":[28,56,59,86,111],"study":[29],"phosphorylation":[31,95,131,182],"channels":[34,68,133,144,186],"intact":[36,188],"cells.":[37],"Rat":[38],"cells":[40,119],"collected":[41],"at":[42,155],"embryonic":[43],"day":[44],"15":[45],"maintained":[47],"culture":[49],"for":[50],"approximately":[51],"21":[52],"days":[53],"were":[54,64,69],"subjected":[55],"treatments":[57],"designed":[58],"increase":[60],"intracellular":[61,196],"cAMP.":[62,199],"Cells":[63],"solubilized":[65],"isolated":[70],"immunoprecipitation,":[72],"then":[73],"rephosphorylated":[74],"with":[75,120],"catalytic":[77],"/sup":[84,90,103,168],"32/P-ATP,":[85],"allow":[87],"incorporation":[88,170],"32/P":[91,104,169],"into":[92,106,171],"available":[93],"sites":[96],"channels.":[99,173],"amount":[101],"incorporated":[105],"inversely":[109],"proportional":[110],"extent":[113,179],"endogenous":[115,130],"phosphorylation.":[116],"Treatment":[117],"forskolin":[121,139],"inhibited":[122],"rephosphorylation":[123],"channels,":[126],"indicating":[127],"that":[128,177],"enhanced":[129],"had":[134],"occurred.":[135],"effect":[137],"on":[140],"cell":[141],"surface":[142],"occurred":[145],"rapidly,":[146],"was":[147,153],"sustained":[148],"over":[149],"30":[150],"min.,":[151],"half-maximal":[154],"6..mu..M.":[156],"8-Br-cAMP,":[157],"(EC/sub":[158,162],"50/-5mM)":[159],"isobutylmethylxanthine":[161],"50/-60..mu..M)":[163],"also":[164],"caused":[165],"inhibition":[166],"These":[174],"results":[175],"indicate":[176],"neurons":[190],"modified":[192],"changes":[194],"levels":[197]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2021-03-15T00:00:00"}