{"id":"https://openalex.org/W3133445927","doi":null,"title":"Obtaining and characterization of a recombinant LipL32 protein for detection of leptospirosis.","display_name":"Obtaining and characterization of a recombinant LipL32 protein for detection of leptospirosis.","publication_year":2018,"publication_date":"2018-03-01","ids":{"openalex":"https://openalex.org/W3133445927","mag":"3133445927","pmid":"https://pubmed.ncbi.nlm.nih.gov/33601801"},"language":"en","primary_location":{"id":"pmid:33601801","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33601801","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Tropical biomedicine","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5037778249","display_name":"Assylbek A. Zhylkibayev","orcid":"https://orcid.org/0000-0001-9244-1505","observed_orcids":["https://orcid.org/0000-0001-9244-1505"]},"institutions":[{"id":"https://openalex.org/I4210101002","display_name":"National Center for Biotechnology","ror":"https://ror.org/00xhcc696","country_code":"KZ","type":"facility","lineage":["https://openalex.org/I4210101002"]}],"countries":["KZ"],"is_corresponding":false,"raw_author_name":"A Zhylkibayev","raw_affiliation_strings":["National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I4210101002"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013005716","display_name":"Zhiger Akishev","orcid":"https://orcid.org/0000-0001-9943-1625","observed_orcids":["https://orcid.org/0000-0001-9943-1625"]},"institutions":[{"id":"https://openalex.org/I4210101002","display_name":"National Center for Biotechnology","ror":"https://ror.org/00xhcc696","country_code":"KZ","type":"facility","lineage":["https://openalex.org/I4210101002"]}],"countries":["KZ"],"is_corresponding":false,"raw_author_name":"Z Akishev","raw_affiliation_strings":["National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I4210101002"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012008768","display_name":"Bekbolat Khassenov","orcid":"https://orcid.org/0000-0003-4572-948X","observed_orcids":["https://orcid.org/0000-0003-4572-948X"]},"institutions":[{"id":"https://openalex.org/I4210101002","display_name":"National Center for Biotechnology","ror":"https://ror.org/00xhcc696","country_code":"KZ","type":"facility","lineage":["https://openalex.org/I4210101002"]}],"countries":["KZ"],"is_corresponding":false,"raw_author_name":"B Khassenov","raw_affiliation_strings":["National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I4210101002"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075797988","display_name":"Nurgul Sarina","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210101002","display_name":"National Center for Biotechnology","ror":"https://ror.org/00xhcc696","country_code":"KZ","type":"facility","lineage":["https://openalex.org/I4210101002"]}],"countries":["KZ"],"is_corresponding":false,"raw_author_name":"N Sarina","raw_affiliation_strings":["National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I4210101002"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033101316","display_name":"Yerlan M. Ramankulov","orcid":"https://orcid.org/0000-0001-6786-3028","observed_orcids":["https://orcid.org/0000-0001-6786-3028"]},"institutions":[{"id":"https://openalex.org/I4210101002","display_name":"National Center for Biotechnology","ror":"https://ror.org/00xhcc696","country_code":"KZ","type":"facility","lineage":["https://openalex.org/I4210101002"]},{"id":"https://openalex.org/I60559429","display_name":"Nazarbayev University","ror":"https://ror.org/052bx8q98","country_code":"KZ","type":"education","lineage":["https://openalex.org/I60559429"]}],"countries":["KZ"],"is_corresponding":false,"raw_author_name":"Y Ramankulov","raw_affiliation_strings":["National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","Nazarbayev University, School of Science and Technology, 53 Kabanbai batyra Avenue, Astana, Kazakhstan, 010000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I4210101002"]},{"raw_affiliation_string":"Nazarbayev University, School of Science and Technology, 53 Kabanbai batyra Avenue, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I60559429"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066206005","display_name":"\u041a.\u041a. \u041c\u0443\u043a\u0430\u043d\u043e\u0432","orcid":"https://orcid.org/0000-0002-0502-9238","observed_orcids":["https://orcid.org/0000-0002-0502-9238"]},"institutions":[{"id":"https://openalex.org/I4210101002","display_name":"National Center for Biotechnology","ror":"https://ror.org/00xhcc696","country_code":"KZ","type":"facility","lineage":["https://openalex.org/I4210101002"]}],"countries":["KZ"],"is_corresponding":false,"raw_author_name":"K Mukanov","raw_affiliation_strings":["National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I4210101002"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5032530962","display_name":"Saule Z. Eskendirova","orcid":"https://orcid.org/0000-0002-9570-7433","observed_orcids":["https://orcid.org/0000-0002-9570-7433"]},"institutions":[{"id":"https://openalex.org/I4210101002","display_name":"National Center for Biotechnology","ror":"https://ror.org/00xhcc696","country_code":"KZ","type":"facility","lineage":["https://openalex.org/I4210101002"]}],"countries":["KZ"],"is_corresponding":false,"raw_author_name":"S Eskendirova","raw_affiliation_strings":["National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, Kazakhstan, 010000","institution_ids":["https://openalex.org/I4210101002"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3817,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.58372255,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"35","issue":"1","first_page":"280","last_page":"287"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12223","display_name":"Leptospirosis research and findings","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12223","display_name":"Leptospirosis research and findings","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10823","display_name":"Research on Leishmaniasis Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11899","display_name":"Brucella: diagnosis, epidemiology, treatment","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/leptospirosis","display_name":"leptospirosis","score":0.9490000009536743},{"id":"https://openalex.org/keywords/lipl32","display_name":"LipL32","score":0.9710000157356262},{"id":"https://openalex.org/keywords/outer-membrane-lipoprotein","display_name":"outer membrane lipoprotein","score":0.8519999980926514},{"id":"https://openalex.org/keywords/recombinant-protein","display_name":"recombinant protein","score":0.8429999947547913},{"id":"https://openalex.org/keywords/leptospira","display_name":"Leptospira","score":0.9169999957084656},{"id":"https://openalex.org/keywords/elisa","display_name":"ELISA","score":0.5299999713897705},{"id":"https://openalex.org/keywords/indirect-elisa","display_name":"indirect ELISA","score":0.871999979019165},{"id":"https://openalex.org/keywords/microscopic-agglutination-test","display_name":"microscopic agglutination test","score":0.9139999747276306},{"id":"https://openalex.org/keywords/diagnostic-antigen","display_name":"diagnostic antigen","score":0.5139999985694885},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.593999981880188},{"id":"https://openalex.org/keywords/antibody-reactivity","display_name":"antibody reactivity","score":0.5540000200271606}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.904304027557373},{"id":"https://openalex.org/C2780542935","wikidata":"https://www.wikidata.org/wiki/Q133841","display_name":"Leptospira","level":3,"score":0.9009045362472534},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.726891040802002},{"id":"https://openalex.org/C2778033228","wikidata":"https://www.wikidata.org/wiki/Q155098","display_name":"Leptospirosis","level":2,"score":0.6763353943824768},{"id":"https://openalex.org/C10389963","wikidata":"https://www.wikidata.org/wiki/Q848328","display_name":"Serotype","level":2,"score":0.6430627107620239},{"id":"https://openalex.org/C203750385","wikidata":"https://www.wikidata.org/wiki/Q1308970","display_name":"Inclusion bodies","level":4,"score":0.6065790057182312},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.537045419216156},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.5293396711349487},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.511111319065094},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.42687657475471497},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4179288148880005},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.37721800804138184},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3243862986564636},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12662667036056519},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.10772541165351868}],"mesh":[],"locations_count":2,"locations":[{"id":"pmid:33601801","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33601801","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Tropical biomedicine","raw_type":"Journal Article"},{"id":"mag:3133445927","doi":null,"is_oa":false,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pubmed/33601801","pdf_url":null,"source":{"id":"https://openalex.org/S50721268","display_name":"Tropical biomedicine","issn_l":"0127-5720","issn":["0127-5720","2521-9855"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","medline","poland-40","russia-white-list-4"],"host_organization":"https://openalex.org/P4362724905","host_organization_name":"Malaysian Society of Parasitology and Tropical Medicine","host_organization_lineage":["https://openalex.org/P4362724905"],"host_organization_lineage_names":["Malaysian Society of Parasitology and Tropical Medicine"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Tropical biomedicine","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2409948030","https://openalex.org/W7198180593","https://openalex.org/W2375206757","https://openalex.org/W2267042190","https://openalex.org/W2366547837","https://openalex.org/W2261434375","https://openalex.org/W7117672409","https://openalex.org/W1579945027","https://openalex.org/W4399049345","https://openalex.org/W2379367524"],"abstract_inverted_index":{"Leptospirosis":[0],"is":[1,21],"a":[2,58,85,128],"disease":[3],"which":[4],"affects":[5],"domestic":[6],"and":[7,41,77,120],"wild":[8],"animals":[9],"as":[10,12],"well":[11],"humans.":[13],"A":[14],"highly":[15],"conserved":[16],"outer":[17],"membrane":[18],"lipoprotein":[19],"LipL32":[20,38,49,59,91,110],"considered":[22],"to":[23,73,125],"be":[24],"an":[25,112],"important":[26],"antigen":[27],"for":[28,61],"detection":[29],"of":[30,88,97,107,127],"leptospirosis.":[31],"In":[32],"this":[33],"study":[34,102],"the":[35,75,89,104,108],"recombinant":[36,47,90,109],"protein":[37,48,76],"was":[39,50,71,118,123],"extracted":[40],"characterized":[42],"by":[43],"immunochemical":[44],"methods.":[45],"The":[46,115],"produced":[51],"in":[52,63,111],"Escherichia":[53],"coli.":[54],"We":[55],"have":[56],"optimized":[57],"gene":[60],"expression":[62],"Rosetta":[64],"(DE3)":[65],"strain.":[66],"0.5%":[67],"sodium":[68],"lauryl":[69],"sulfate":[70],"used":[72],"solubilize":[74],"expressed":[78],"into":[79],"inclusion":[80],"bodies.":[81],"Immunochemical":[82],"studies":[83],"demonstrated":[84],"high":[86],"reactivity":[87],"with":[92],"antibodies":[93],"elicited":[94],"during":[95],"infection":[96],"pathogenic":[98],"Leptospira":[99],"serovars.":[100],"This":[101],"tested":[103],"diagnostic":[105],"performance":[106],"in-house":[113],"ELISA.":[114],"indirect":[116],"ELISA":[117],"developed":[119],"its":[121],"specificity":[122],"compared":[124],"that":[126],"traditional":[129],"microagglutination":[130],"test":[131],"(MAT).":[132]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2021-03-01T00:00:00"}