{"id":"https://openalex.org/W3130125709","doi":"https://doi.org/10.3390/ijms22052249","title":"One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis","display_name":"One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis","publication_year":2021,"publication_date":"2021-02-24","ids":{"openalex":"https://openalex.org/W3130125709","doi":"https://doi.org/10.3390/ijms22052249","mag":"3130125709","pmid":"https://pubmed.ncbi.nlm.nih.gov/33668187","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7956194"},"language":"en","primary_location":{"id":"doi:10.3390/ijms22052249","doi":"https://doi.org/10.3390/ijms22052249","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms22052249","pdf_url":"https://www.mdpi.com/1422-0067/22/5/2249/pdf?version=1614663798","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1422-0067/22/5/2249/pdf?version=1614663798","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007605346","display_name":"Fuminori Tanihara","orcid":"https://orcid.org/0000-0002-2158-5808","observed_orcids":["https://orcid.org/0000-0002-2158-5808"]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Fuminori Tanihara","raw_affiliation_strings":["Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037216682","display_name":"Maki Hirata","orcid":"https://orcid.org/0000-0003-4673-1581","observed_orcids":["https://orcid.org/0000-0003-4673-1581"]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Maki Hirata","raw_affiliation_strings":["Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103864227","display_name":"Nhien Thi Nguyen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Nhien Thi Nguyen","raw_affiliation_strings":["Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050342671","display_name":"Osamu Sawamoto","orcid":"https://orcid.org/0000-0002-1756-8829","observed_orcids":["https://orcid.org/0000-0002-1756-8829"]},"institutions":[{"id":"https://openalex.org/I114030911","display_name":"Otsuka (Japan)","ror":"https://ror.org/013k5y296","country_code":"JP","type":"company","lineage":["https://openalex.org/I114030911"]},{"id":"https://openalex.org/I4405271247","display_name":"Otsuka Pharmaceutical Factory (Japan)","ror":"https://ror.org/024hzjk66","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405271247"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Osamu Sawamoto","raw_affiliation_strings":["Research and Development Center, Otsuka Pharmaceutical Factory, Inc., 115 Muya-cho, Naruto, Tokushima 772-8601, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research and Development Center, Otsuka Pharmaceutical Factory, Inc., 115 Muya-cho, Naruto, Tokushima 772-8601, Japan","institution_ids":["https://openalex.org/I114030911","https://openalex.org/I4405271247"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061553906","display_name":"Takeshi Kikuchi","orcid":"https://orcid.org/0000-0002-8063-0293","observed_orcids":["https://orcid.org/0000-0002-8063-0293"]},"institutions":[{"id":"https://openalex.org/I114030911","display_name":"Otsuka (Japan)","ror":"https://ror.org/013k5y296","country_code":"JP","type":"company","lineage":["https://openalex.org/I114030911"]},{"id":"https://openalex.org/I4405271247","display_name":"Otsuka Pharmaceutical Factory (Japan)","ror":"https://ror.org/024hzjk66","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405271247"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takeshi Kikuchi","raw_affiliation_strings":["Research and Development Center, Otsuka Pharmaceutical Factory, Inc., 115 Muya-cho, Naruto, Tokushima 772-8601, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research and Development Center, Otsuka Pharmaceutical Factory, Inc., 115 Muya-cho, Naruto, Tokushima 772-8601, Japan","institution_ids":["https://openalex.org/I114030911","https://openalex.org/I4405271247"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5053502532","display_name":"Takeshige Otoi","orcid":"https://orcid.org/0000-0002-0382-959X","observed_orcids":["https://orcid.org/0000-0002-0382-959X"]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Takeshige Otoi","raw_affiliation_strings":["Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan","institution_ids":["https://openalex.org/I922474255"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5053502532"],"corresponding_institution_ids":["https://openalex.org/I922474255"],"apc_list":{"value":2000,"currency":"CHF","value_usd":2160},"apc_paid":{"value":2000,"currency":"CHF","value_usd":2160},"fwci":6.1517,"has_fulltext":true,"cited_by_count":40,"citation_normalized_percentile":{"value":0.97561128,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":92,"max":99},"biblio":{"volume":"22","issue":"5","first_page":"2249","last_page":"2249"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12654","display_name":"Xenotransplantation and immune response","score":0.8202999830245972,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12654","display_name":"Xenotransplantation and immune response","score":0.8202999830245972,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.09529999643564224,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.08229999989271164,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.796999990940094},{"id":"https://openalex.org/keywords/gene-edited-pigs","display_name":"gene-edited pigs","score":0.7730000019073486},{"id":"https://openalex.org/keywords/ggta1","display_name":"GGTA1","score":0.8999999761581421},{"id":"https://openalex.org/keywords/cmah","display_name":"CMAH","score":1.0},{"id":"https://openalex.org/keywords/b4galnt2","display_name":"B4GALNT2","score":1.0},{"id":"https://openalex.org/keywords/in-vitro-fertilization","display_name":"in vitro fertilization","score":0.6669999957084656},{"id":"https://openalex.org/keywords/xenotransplantation","display_name":"xenotransplantation","score":0.8080000281333923},{"id":"https://openalex.org/keywords/hyperacute-rejection","display_name":"hyperacute rejection","score":0.7319999933242798},{"id":"https://openalex.org/keywords/genetic-mosaicism","display_name":"genetic mosaicism","score":0.8939999938011169}],"concepts":[{"id":"https://openalex.org/C2778442404","wikidata":"https://www.wikidata.org/wiki/Q55505","display_name":"Xenotransplantation","level":3,"score":0.9084438681602478},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8362325429916382},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.7783564329147339},{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.7667945623397827},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6561226844787598},{"id":"https://openalex.org/C28748434","wikidata":"https://www.wikidata.org/wiki/Q170145","display_name":"Zygote","level":4,"score":0.5874658823013306},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.5786541700363159},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4886327385902405},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3592236638069153},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.330163836479187},{"id":"https://openalex.org/C2911091166","wikidata":"https://www.wikidata.org/wiki/Q106419912","display_name":"Transplantation","level":2,"score":0.2753106355667114},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.09627571702003479},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.0788978636264801},{"id":"https://openalex.org/C141071460","wikidata":"https://www.wikidata.org/wiki/Q40821","display_name":"Surgery","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005700","descriptor_name":"Galactosyltransferases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015053","descriptor_name":"Zygote","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D015478","descriptor_name":"Antigens, Heterophile","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D017350","descriptor_name":"N-Acetylgalactosaminyltransferases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D030801","descriptor_name":"Animals, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.3390/ijms22052249","doi":"https://doi.org/10.3390/ijms22052249","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms22052249","pdf_url":"https://www.mdpi.com/1422-0067/22/5/2249/pdf?version=1614663798","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},{"id":"pmid:33668187","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33668187","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of molecular sciences","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7956194","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC7956194/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Mol Sci","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:7dd4f8b9b75d4433a2f66250df308d43","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/7dd4f8b9b75d4433a2f66250df308d43","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"International Journal of Molecular Sciences, Vol 22, Iss 2249, p 2249 (2021)","raw_type":"article"},{"id":"pmh:oai:irdb.nii.ac.jp:00906:0006781169","doi":null,"is_oa":true,"landing_page_url":"https://tokushima-u.repo.nii.ac.jp/records/2009256","pdf_url":null,"source":{"id":"https://openalex.org/S7407056385","display_name":"Institutional Repositories DataBase (IRDB)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I184597095","host_organization_name":"National Institute of Informatics","host_organization_lineage":["https://openalex.org/I184597095"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal article"}],"best_oa_location":{"id":"doi:10.3390/ijms22052249","doi":"https://doi.org/10.3390/ijms22052249","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms22052249","pdf_url":"https://www.mdpi.com/1422-0067/22/5/2249/pdf?version=1614663798","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"},{"id":"https://metadata.un.org/sdg/2","score":0.40450000762939453,"display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1629297356","display_name":"Generation of a pig model for the diabetic nephropathy using gene editing technology","funder_award_id":"19K16014","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G2538344913","display_name":"Production of immunodeficient micro-mini pig using genome editing technology.","funder_award_id":"18K12062","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G4900688645","display_name":"\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u3088\u308b\u9769\u65b0\u7684\u306a\u6709\u7528\u7d30\u80de\u30fb\u751f\u7269\u4f5c\u6210\u6280\u8853\u306e\u5275\u51fa","funder_award_id":"JPMJOP1613","funder_id":"https://openalex.org/F4320334789","funder_display_name":"Japan Science and Technology Agency"}],"funders":[{"id":"https://openalex.org/F4320320912","display_name":"Ministry of Education, Culture, Sports, Science and Technology","ror":"https://ror.org/048rj2z13"},{"id":"https://openalex.org/F4320323325","display_name":"University of Tokushima","ror":"https://ror.org/044vy1d05"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"},{"id":"https://openalex.org/F4320334789","display_name":"Japan Science and Technology Agency","ror":"https://ror.org/00097mb19"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3130125709.pdf","grobid_xml":"https://content.openalex.org/works/W3130125709.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1613626423","https://openalex.org/W1618744733","https://openalex.org/W1969345130","https://openalex.org/W1975897697","https://openalex.org/W1976507427","https://openalex.org/W1982831423","https://openalex.org/W1986094205","https://openalex.org/W2007818617","https://openalex.org/W2011525960","https://openalex.org/W2035139389","https://openalex.org/W2066735040","https://openalex.org/W2091140839","https://openalex.org/W2106070783","https://openalex.org/W2107773664","https://openalex.org/W2126605149","https://openalex.org/W2145782793","https://openalex.org/W2170039745","https://openalex.org/W2288966576","https://openalex.org/W2462655722","https://openalex.org/W2463680085","https://openalex.org/W2520545223","https://openalex.org/W2575274634","https://openalex.org/W2598886315","https://openalex.org/W2725816324","https://openalex.org/W2769511352","https://openalex.org/W2795021051","https://openalex.org/W2795688422","https://openalex.org/W2804655893","https://openalex.org/W2809537749","https://openalex.org/W2883161041","https://openalex.org/W2886819502","https://openalex.org/W2896848238","https://openalex.org/W2898974045","https://openalex.org/W2968982657","https://openalex.org/W2972264072","https://openalex.org/W2979944588","https://openalex.org/W2989506038","https://openalex.org/W3010844626","https://openalex.org/W3013504741","https://openalex.org/W3033008666","https://openalex.org/W3033887021","https://openalex.org/W3037444933","https://openalex.org/W3038956601","https://openalex.org/W3070413182","https://openalex.org/W3088203646","https://openalex.org/W3120612032","https://openalex.org/W3146125541"],"related_works":["https://openalex.org/W3070413182","https://openalex.org/W2511115675","https://openalex.org/W4387877413","https://openalex.org/W1509485701","https://openalex.org/W2883161041","https://openalex.org/W4409207200","https://openalex.org/W2558253542","https://openalex.org/W2072070315","https://openalex.org/W2901021294","https://openalex.org/W2064765186"],"abstract_inverted_index":{"Xenoantigens":[0],"cause":[1],"hyperacute":[2],"rejection":[3],"and":[4,21,44,73,83,91,113,118,135],"limit":[5],"the":[6,28,53,65,88,99,106,123,128,142,166,175],"success":[7],"of":[8,30,56,67,131,144,178],"interspecific":[9],"xenografts.":[10],"Therefore,":[11],"genes":[12],"involved":[13],"in":[14,47,81,182],"xenoantigen":[15],"biosynthesis,":[16],"such":[17],"as":[18],"GGTA1,":[19,42,111],"CMAH,":[20,43,112],"B4GALNT2,":[22],"are":[23],"key":[24],"targets":[25],"to":[26,77,105,156],"improve":[27],"outcomes":[29],"xenotransplantation.":[31,188],"In":[32],"this":[33],"study,":[34],"we":[35,63,97],"introduced":[36],"a":[37,171],"CRISPR/Cas9":[38],"system":[39],"simultaneously":[40],"targeting":[41,71],"B4GALNT2":[45,114],"into":[46,93],"vitro-fertilized":[48],"zygotes":[49],"using":[50,122],"electroporation":[51,167],"for":[52,174],"one-step":[54,129,176],"generation":[55,130,177],"multiple":[57,148,179],"gene-edited":[58,120,149],"pigs":[59,121,134,150,183],"without":[60],"xenoantigens.":[61,160],"First,":[62],"optimized":[64,89,98,124],"combination":[66],"guide":[68],"RNAs":[69],"(gRNAs)":[70],"GGTA1":[72],"CMAH":[74],"with":[75,87,103],"respect":[76,104],"gene":[78,107,180],"editing":[79,108],"efficiency":[80,109],"zygotes,":[82],"transferred":[84],"electroporated":[85],"embryos":[86],"gRNAs":[90],"Cas9":[92,100],"recipient":[94],"gilts.":[95],"Next,":[96],"protein":[101],"concentration":[102],"when":[110],"were":[115,151],"targeted":[116],"simultaneously,":[117],"generated":[119],"conditions.":[125],"We":[126],"achieved":[127],"GGTA1/CMAH":[132],"double-edited":[133],"GGTA1/CMAH/B4GALNT2":[136],"triple-edited":[137],"pigs.":[138],"Immunohistological":[139],"analyses":[140],"demonstrated":[141],"downregulation":[143],"xenoantigens;":[145],"however,":[146],"these":[147],"genetic":[152],"mosaics":[153],"that":[154],"failed":[155],"knock":[157],"out":[158],"some":[159],"Although":[161],"mosaicism":[162],"should":[163],"be":[164],"resolved,":[165],"technique":[168],"could":[169],"become":[170],"primary":[172],"method":[173],"modifications":[181],"aimed":[184],"at":[185],"improving":[186],"pig-to-human":[187]},"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":11},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2021-03-01T00:00:00"}