{"id":"https://openalex.org/W3122813686","doi":"https://doi.org/10.1055/s-0040-1706122","title":"Directed Evolution of an Acyltransferase to Expand Its Substrate Recognition to Thiols and Secondary Amines","display_name":"Directed Evolution of an Acyltransferase to Expand Its Substrate Recognition to Thiols and Secondary Amines","publication_year":2021,"publication_date":"2021-01-20","ids":{"openalex":"https://openalex.org/W3122813686","doi":"https://doi.org/10.1055/s-0040-1706122","mag":"3122813686"},"language":"en","primary_location":{"id":"doi:10.1055/s-0040-1706122","doi":"https://doi.org/10.1055/s-0040-1706122","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0040-1706122","pdf_url":null,"source":{"id":"https://openalex.org/S135102583","display_name":"Synfacts","issn_l":"1861-194X","issn":["1861-194X","1861-1958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310321135","host_organization_name":"Georg Thieme Verlag","host_organization_lineage":["https://openalex.org/P4310321135","https://openalex.org/P4310320000"],"host_organization_lineage_names":["Georg Thieme Verlag","Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Synfacts","raw_type":"journal-article"},"volume":null,"type":"editorial","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040055598","display_name":"Benjamin List","orcid":"https://orcid.org/0000-0002-9804-599X","observed_orcids":["https://orcid.org/0000-0002-9804-599X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Benjamin List","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5019758746","display_name":"Oleg Grossmann","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Oleg Grossmann","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"17","issue":"02","first_page":"0204","last_page":"0204"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11831","display_name":"Chemical Synthesis and Reactions","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acyltransferase","display_name":"acyltransferase","score":0.8769999742507935},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"directed evolution","score":0.9300000071525574},{"id":"https://openalex.org/keywords/substrate-recognition","display_name":"substrate recognition","score":0.8320000171661377},{"id":"https://openalex.org/keywords/thioesters","display_name":"thioesters","score":0.6970000267028809},{"id":"https://openalex.org/keywords/tertiary-amides","display_name":"tertiary amides","score":0.984000027179718},{"id":"https://openalex.org/keywords/catalytic-triad","display_name":"catalytic triad","score":0.925000011920929},{"id":"https://openalex.org/keywords/mycobacterium-smegmatis","display_name":"Mycobacterium smegmatis","score":0.9900000095367432}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9131488800048828},{"id":"https://openalex.org/C2910079589","wikidata":"https://www.wikidata.org/wiki/Q2609152","display_name":"Acyltransferases","level":4,"score":0.8333615660667419},{"id":"https://openalex.org/C2775970874","wikidata":"https://www.wikidata.org/wiki/Q2609152","display_name":"Acyltransferase","level":3,"score":0.7157686948776245},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.6655370593070984},{"id":"https://openalex.org/C2777204207","wikidata":"https://www.wikidata.org/wiki/Q606230","display_name":"Mycobacterium smegmatis","level":4,"score":0.645949125289917},{"id":"https://openalex.org/C2994592520","wikidata":"https://www.wikidata.org/wiki/Q5090496","display_name":"Substrate specificity","level":3,"score":0.6110692024230957},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.41316890716552734},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39874228835105896},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.38527506589889526},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3606838881969452},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.32792574167251587},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.08465120196342468},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.07310625910758972},{"id":"https://openalex.org/C2777975735","wikidata":"https://www.wikidata.org/wiki/Q130971","display_name":"Mycobacterium tuberculosis","level":3,"score":0.06915920972824097},{"id":"https://openalex.org/C2781069245","wikidata":"https://www.wikidata.org/wiki/Q12204","display_name":"Tuberculosis","level":2,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1055/s-0040-1706122","doi":"https://doi.org/10.1055/s-0040-1706122","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0040-1706122","pdf_url":null,"source":{"id":"https://openalex.org/S135102583","display_name":"Synfacts","issn_l":"1861-194X","issn":["1861-194X","1861-1958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310321135","host_organization_name":"Georg Thieme Verlag","host_organization_lineage":["https://openalex.org/P4310321135","https://openalex.org/P4310320000"],"host_organization_lineage_names":["Georg Thieme Verlag","Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Synfacts","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W3109301630"],"related_works":["https://openalex.org/W2967848109","https://openalex.org/W7222653990","https://openalex.org/W3035192066","https://openalex.org/W2270678330","https://openalex.org/W7126190922","https://openalex.org/W7028089309","https://openalex.org/W1993110082","https://openalex.org/W2100357455","https://openalex.org/W2897448209","https://openalex.org/W2921099067"],"abstract_inverted_index":{"Key":[0],"words":[1],"catalytic":[2],"triads":[3],"-":[4,6,9,12,14],"acyltransferases":[5],"directed":[7],"evolution":[8],"Mycobacterium":[10],"smegmatis":[11],"thioesters":[13],"tertiary":[15],"amides":[16],"Publication":[17,19],"History":[18],"Date:":[20],"20":[21],"January":[22],"2021":[23],"(online)":[24],"\u00a9":[25],"2021.":[26],"Thieme.":[27],"All":[28],"rights":[29],"reserved":[30],"Georg":[31],"Thieme":[32],"Verlag":[33],"KG":[34],"R\u00fcdigerstra\u00dfe":[35],"14,":[36],"70469":[37],"Stuttgart,":[38],"Germany":[39]},"counts_by_year":[],"updated_date":"2026-07-14T23:27:15.235271","created_date":"2021-02-01T00:00:00"}