{"id":"https://openalex.org/W3118411684","doi":"https://doi.org/10.1101/2021.01.13.426577","title":"Microglia perform local protein synthesis at perisynaptic and phagocytic structures","display_name":"Microglia perform local protein synthesis at perisynaptic and phagocytic structures","publication_year":2021,"publication_date":"2021-01-15","ids":{"openalex":"https://openalex.org/W3118411684","doi":"https://doi.org/10.1101/2021.01.13.426577","mag":"3118411684"},"language":"en","primary_location":{"id":"doi:10.1101/2021.01.13.426577","doi":"https://doi.org/10.1101/2021.01.13.426577","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2021.01.13.426577","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2021/01/15/2021.01.13.426577.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2021/01/15/2021.01.13.426577.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5041943054","display_name":"Michael J. Vasek","orcid":"https://orcid.org/0000-0002-1112-4346","observed_orcids":["https://orcid.org/0000-0002-1112-4346"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael J. Vasek","raw_affiliation_strings":["Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","Department of Psychiatry, Washington University School of Medicine","Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA"],"raw_orcid":"https://orcid.org/0000-0002-1112-4346","affiliations":[{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","institution_ids":["https://openalex.org/I204465549"]},{"raw_affiliation_string":"Department of Psychiatry, Washington University School of Medicine","institution_ids":[]},{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033573076","display_name":"Jelani D. Deajon-Jackson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jelani D. Deajon-Jackson","raw_affiliation_strings":["Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","Department of Psychiatry, Washington University School of Medicine","Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","institution_ids":["https://openalex.org/I204465549"]},{"raw_affiliation_string":"Department of Psychiatry, Washington University School of Medicine","institution_ids":[]},{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100727170","display_name":"Yating Liu","orcid":"https://orcid.org/0000-0001-7425-0422","observed_orcids":["https://orcid.org/0000-0001-7425-0422","https://orcid.org/0009-0008-5688-7233"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yating Liu","raw_affiliation_strings":["Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","Department of Psychiatry, Washington University School of Medicine","Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","institution_ids":["https://openalex.org/I204465549"]},{"raw_affiliation_string":"Department of Psychiatry, Washington University School of Medicine","institution_ids":[]},{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014876663","display_name":"Haley W. Crosby","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haley W. Crosby","raw_affiliation_strings":["Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","Department of Psychiatry, Washington University School of Medicine","Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","institution_ids":["https://openalex.org/I204465549"]},{"raw_affiliation_string":"Department of Psychiatry, Washington University School of Medicine","institution_ids":[]},{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065690117","display_name":"Jiwon Yi","orcid":"https://orcid.org/0000-0002-5869-8566","observed_orcids":["https://orcid.org/0000-0002-5869-8566","https://orcid.org/0009-0004-6145-8370"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jiwon Yi","raw_affiliation_strings":["Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","Department of Psychiatry, Washington University School of Medicine","Division of Biology and Biomedical Sciences, Washington University School of Medicine","Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","institution_ids":["https://openalex.org/I204465549"]},{"raw_affiliation_string":"Department of Psychiatry, Washington University School of Medicine","institution_ids":[]},{"raw_affiliation_string":"Division of Biology and Biomedical Sciences, Washington University School of Medicine","institution_ids":["https://openalex.org/I204465549"]},{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089145425","display_name":"Joseph D. Dougherty","orcid":"https://orcid.org/0000-0002-6385-3997","observed_orcids":["https://orcid.org/0000-0002-6385-3997"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joseph D. Dougherty","raw_affiliation_strings":["Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","Department of Psychiatry, Washington University School of Medicine","Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA"],"raw_orcid":"https://orcid.org/0000-0002-6385-3997","affiliations":[{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 63108, USA","institution_ids":["https://openalex.org/I204465549"]},{"raw_affiliation_string":"Department of Psychiatry, Washington University School of Medicine","institution_ids":[]},{"raw_affiliation_string":"Department of Genetics, Washington University School of Medicine, 660 S. Euclid Ave, Saint Louis MO, 6308, USA","institution_ids":["https://openalex.org/I204465549"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5089145425"],"corresponding_institution_ids":["https://openalex.org/I204465549"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":9,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microglia","display_name":"microglia","score":0.9980000257492065},{"id":"https://openalex.org/keywords/local-protein-synthesis","display_name":"local protein synthesis","score":0.8700000047683716},{"id":"https://openalex.org/keywords/phagocytosis","display_name":"phagocytosis","score":0.9670000076293945},{"id":"https://openalex.org/keywords/phagolysosomes","display_name":"phagolysosomes","score":0.7549999952316284},{"id":"https://openalex.org/keywords/phagocytic-cup","display_name":"phagocytic cup","score":0.8180000185966492},{"id":"https://openalex.org/keywords/ribosome","display_name":"ribosome","score":0.7250000238418579},{"id":"https://openalex.org/keywords/lysosomal-proteins","display_name":"lysosomal proteins","score":0.8240000009536743},{"id":"https://openalex.org/keywords/pathogen-defense","display_name":"pathogen defense","score":0.6019999980926514},{"id":"https://openalex.org/keywords/microglial-motility","display_name":"microglial motility","score":0.8149999976158142}],"concepts":[{"id":"https://openalex.org/C160448771","wikidata":"https://www.wikidata.org/wiki/Q184726","display_name":"Phagocytosis","level":2,"score":0.8969565629959106},{"id":"https://openalex.org/C2779830541","wikidata":"https://www.wikidata.org/wiki/Q1622829","display_name":"Microglia","level":3,"score":0.8758971691131592},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.7040883898735046},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6238798499107361},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.5780230164527893},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.5166943073272705},{"id":"https://openalex.org/C58207958","wikidata":"https://www.wikidata.org/wiki/Q33596","display_name":"Motility","level":2,"score":0.5101594924926758},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47128668427467346},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.4632182717323303},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.23393785953521729},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.2282690405845642},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.1467420756816864},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13484451174736023},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10231167078018188},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.07833892107009888}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/2021.01.13.426577","doi":"https://doi.org/10.1101/2021.01.13.426577","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2021.01.13.426577","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2021/01/15/2021.01.13.426577.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1101/2021.01.13.426577","doi":"https://doi.org/10.1101/2021.01.13.426577","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2021.01.13.426577","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2021/01/15/2021.01.13.426577.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320333635","display_name":"Center for Cellular Imaging, Washington University","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3118411684.pdf","grobid_xml":"https://content.openalex.org/works/W3118411684.grobid-xml"},"referenced_works_count":53,"referenced_works":["https://openalex.org/W68733535","https://openalex.org/W238913303","https://openalex.org/W1870286356","https://openalex.org/W1976500290","https://openalex.org/W1979496428","https://openalex.org/W1994709846","https://openalex.org/W2022174079","https://openalex.org/W2025886611","https://openalex.org/W2027767007","https://openalex.org/W2029987169","https://openalex.org/W2033617297","https://openalex.org/W2052359428","https://openalex.org/W2054275187","https://openalex.org/W2058926606","https://openalex.org/W2062464203","https://openalex.org/W2066943555","https://openalex.org/W2075463100","https://openalex.org/W2079838847","https://openalex.org/W2089438839","https://openalex.org/W2094660330","https://openalex.org/W2098073190","https://openalex.org/W2103711259","https://openalex.org/W2105450045","https://openalex.org/W2113755074","https://openalex.org/W2114104545","https://openalex.org/W2122828560","https://openalex.org/W2127802201","https://openalex.org/W2131271579","https://openalex.org/W2134526812","https://openalex.org/W2138267133","https://openalex.org/W2150280084","https://openalex.org/W2163410184","https://openalex.org/W2169456326","https://openalex.org/W2169589032","https://openalex.org/W2170551349","https://openalex.org/W2170899083","https://openalex.org/W2314634699","https://openalex.org/W2471701648","https://openalex.org/W2594676718","https://openalex.org/W2597423760","https://openalex.org/W2730472814","https://openalex.org/W2742814781","https://openalex.org/W2804756252","https://openalex.org/W2885498250","https://openalex.org/W2906673038","https://openalex.org/W2912990441","https://openalex.org/W2921756914","https://openalex.org/W2923898779","https://openalex.org/W2948076969","https://openalex.org/W2951270532","https://openalex.org/W3014938518","https://openalex.org/W3080570440","https://openalex.org/W4244630643"],"related_works":["https://openalex.org/W4379390762","https://openalex.org/W4380434163","https://openalex.org/W4416078623","https://openalex.org/W4385488116","https://openalex.org/W4386819699","https://openalex.org/W3123135744","https://openalex.org/W4224245672","https://openalex.org/W7028457778","https://openalex.org/W4410711724","https://openalex.org/W6948813929"],"abstract_inverted_index":{"Abstract":[0],"Recent":[1],"studies":[2],"have":[3],"illuminated":[4],"the":[5,14,32,46,115,121],"importance":[6],"of":[7,20,34,39,93,117,123],"several":[8],"key":[9],"signaling":[10],"pathways":[11],"in":[12,31,42,82,96,139],"regulating":[13],"dynamic":[15,150],"surveillance":[16],"and":[17,51,87,100,128,141],"phagocytic":[18,119],"activity":[19],"microglia.":[21],"Yet":[22],"little":[23],"is":[24,65],"known":[25],"about":[26],"how":[27],"these":[28,88,131],"signals":[29],"result":[30],"assembly":[33],"phagolysosomal":[35],"machinery":[36],"near":[37],"targets":[38],"phagocytosis,":[40],"especially":[41],"processes":[43,72],"distal":[44,58],"from":[45],"microglial":[47,151],"soma.":[48],"Neurons,":[49],"astrocytes,":[50],"oligodendrocytes":[52],"locally":[53],"regulate":[54],"protein":[55,85],"translation":[56,68,112,138,147],"within":[57,69,126],"processes.":[59],"Therefore,":[60],"we":[61,107],"tested":[62],"whether":[63],"there":[64],"regulated":[66,136],"local":[67,137],"peripheral":[70],"microglia":[71],"(PeMPs).":[73],"We":[74],"show":[75,109],"that":[76,110],"PeMPs":[77,140],"contain":[78],"ribosomes":[79],"which":[80],"engage":[81],"de":[83],"novo":[84],"synthesis,":[86],"associate":[89],"with":[90],"a":[91,103,135,143],"subpool":[92],"transcripts":[94],"involved":[95],"pathogen":[97],"defense,":[98],"motility,":[99],"phagocytosis.":[101,129],"Using":[102],"live":[104],"slice":[105],"preparation,":[106],"further":[108],"acute":[111],"blockade":[113],"impairs":[114],"formation":[116],"PeMP":[118],"cups,":[120],"localization":[122],"lysosomal":[124],"proteins":[125],"them,":[127],"Collectively,":[130],"data":[132],"argue":[133],"for":[134,145],"indicate":[142],"need":[144],"new":[146],"to":[148],"support":[149],"function.":[152]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":2}],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2021-01-18T00:00:00"}