{"id":"https://openalex.org/W3111363876","doi":"https://doi.org/10.1039/d0ay01498b","title":"Complete enzymatic digestion of double-stranded RNA to nucleosides enables accurate quantification of dsRNA","display_name":"Complete enzymatic digestion of double-stranded RNA to nucleosides enables accurate quantification of dsRNA","publication_year":2020,"publication_date":"2020-12-09","ids":{"openalex":"https://openalex.org/W3111363876","doi":"https://doi.org/10.1039/d0ay01498b","mag":"3111363876","pmid":"https://pubmed.ncbi.nlm.nih.gov/33319868"},"language":"en","primary_location":{"id":"doi:10.1039/d0ay01498b","doi":"https://doi.org/10.1039/d0ay01498b","is_oa":true,"landing_page_url":"https://doi.org/10.1039/d0ay01498b","pdf_url":"https://pubs.rsc.org/en/content/articlepdf/2021/ay/d0ay01498b","source":{"id":"https://openalex.org/S170358085","display_name":"Analytical Methods","issn_l":"1759-9660","issn":["1759-9660","1759-9679"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://pubs.rsc.org/en/content/articlepdf/2021/ay/d0ay01498b","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030297626","display_name":"Steven R. Strezsak","orcid":"https://orcid.org/0000-0003-4203-4498","observed_orcids":["https://orcid.org/0000-0003-4203-4498"]},"institutions":[{"id":"https://openalex.org/I12912129","display_name":"Northeastern University","ror":"https://ror.org/04t5xt781","country_code":"US","type":"education","lineage":["https://openalex.org/I12912129"]},{"id":"https://openalex.org/I4210154646","display_name":"GreenLight Biosciences (United States)","ror":"https://ror.org/04zr4fy40","country_code":"US","type":"company","lineage":["https://openalex.org/I4210154646"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven R. Strezsak","raw_affiliation_strings":["Boston","Department of Chemistry & Chemical Biology","Greenlight Biosciences","Northeastern University","USA","Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA","Greenlight Biosciences, Medford, MA, USA"],"raw_orcid":"https://orcid.org/0000-0003-4203-4498","affiliations":[{"raw_affiliation_string":"Boston","institution_ids":[]},{"raw_affiliation_string":"Department of Chemistry & Chemical Biology","institution_ids":[]},{"raw_affiliation_string":"Greenlight Biosciences","institution_ids":["https://openalex.org/I4210154646"]},{"raw_affiliation_string":"Northeastern University","institution_ids":["https://openalex.org/I12912129"]},{"raw_affiliation_string":"USA","institution_ids":[]},{"raw_affiliation_string":"Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA","institution_ids":["https://openalex.org/I12912129"]},{"raw_affiliation_string":"Greenlight Biosciences, Medford, MA, USA","institution_ids":["https://openalex.org/I4210154646"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015910317","display_name":"Penny J. Beuning","orcid":"https://orcid.org/0000-0002-7770-022X","observed_orcids":["https://orcid.org/0000-0002-7770-022X"]},"institutions":[{"id":"https://openalex.org/I12912129","display_name":"Northeastern University","ror":"https://ror.org/04t5xt781","country_code":"US","type":"education","lineage":["https://openalex.org/I12912129"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Penny J. Beuning","raw_affiliation_strings":["Boston","Department of Chemistry & Chemical Biology","Northeastern University","USA","Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boston","institution_ids":[]},{"raw_affiliation_string":"Department of Chemistry & Chemical Biology","institution_ids":[]},{"raw_affiliation_string":"Northeastern University","institution_ids":["https://openalex.org/I12912129"]},{"raw_affiliation_string":"USA","institution_ids":[]},{"raw_affiliation_string":"Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA","institution_ids":["https://openalex.org/I12912129"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048028927","display_name":"Nicholas J. Skizim","orcid":"https://orcid.org/0000-0002-2034-0542","observed_orcids":["https://orcid.org/0000-0002-2034-0542"]},"institutions":[{"id":"https://openalex.org/I4210154646","display_name":"GreenLight Biosciences (United States)","ror":"https://ror.org/04zr4fy40","country_code":"US","type":"company","lineage":["https://openalex.org/I4210154646"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Nicholas J. Skizim","raw_affiliation_strings":["Greenlight Biosciences","Medford","USA","Greenlight Biosciences, Medford, MA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Greenlight Biosciences","institution_ids":["https://openalex.org/I4210154646"]},{"raw_affiliation_string":"Medford","institution_ids":[]},{"raw_affiliation_string":"USA","institution_ids":[]},{"raw_affiliation_string":"Greenlight Biosciences, Medford, MA, USA","institution_ids":["https://openalex.org/I4210154646"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5048028927"],"corresponding_institution_ids":["https://openalex.org/I4210154646"],"apc_list":null,"apc_paid":null,"fwci":0.3243,"has_fulltext":true,"cited_by_count":14,"citation_normalized_percentile":{"value":0.50602318,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"13","issue":"2","first_page":"179","last_page":"185"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.6615999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.6615999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.1582999974489212,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.1062999963760376,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nucleoside-separation","display_name":"nucleoside separation","score":0.5619999766349792},{"id":"https://openalex.org/keywords/extinction-coefficient","display_name":"extinction coefficient","score":0.7599999904632568},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.8159999847412109}],"concepts":[{"id":"https://openalex.org/C2191507","wikidata":"https://www.wikidata.org/wiki/Q7277185","display_name":"RNA silencing","level":5,"score":0.7973422408103943},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.7202120423316956},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.47748348116874695},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4714582562446594},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.46952691674232483},{"id":"https://openalex.org/C2781357212","wikidata":"https://www.wikidata.org/wiki/Q5275608","display_name":"Digestion (alchemy)","level":2,"score":0.45793890953063965},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.455474853515625},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3254877030849457},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3202636241912842},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3115417957305908},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11221766471862793}],"mesh":[{"descriptor_ui":"D004063","descriptor_name":"Digestion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009705","descriptor_name":"Nucleosides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012330","descriptor_name":"RNA, Double-Stranded","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D034622","descriptor_name":"RNA Interference","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1039/d0ay01498b","doi":"https://doi.org/10.1039/d0ay01498b","is_oa":true,"landing_page_url":"https://doi.org/10.1039/d0ay01498b","pdf_url":"https://pubs.rsc.org/en/content/articlepdf/2021/ay/d0ay01498b","source":{"id":"https://openalex.org/S170358085","display_name":"Analytical Methods","issn_l":"1759-9660","issn":["1759-9660","1759-9679"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Methods","raw_type":"journal-article"},{"id":"pmid:33319868","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33319868","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical methods : advancing methods and applications","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1039/d0ay01498b","doi":"https://doi.org/10.1039/d0ay01498b","is_oa":true,"landing_page_url":"https://doi.org/10.1039/d0ay01498b","pdf_url":"https://pubs.rsc.org/en/content/articlepdf/2021/ay/d0ay01498b","source":{"id":"https://openalex.org/S170358085","display_name":"Analytical Methods","issn_l":"1759-9660","issn":["1759-9660","1759-9679"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Methods","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3111363876.pdf","grobid_xml":"https://content.openalex.org/works/W3111363876.grobid-xml"},"referenced_works_count":15,"referenced_works":["https://openalex.org/W1531875369","https://openalex.org/W1996342047","https://openalex.org/W2008488679","https://openalex.org/W2008753573","https://openalex.org/W2028753366","https://openalex.org/W2034537520","https://openalex.org/W2051296345","https://openalex.org/W2052179426","https://openalex.org/W2071542835","https://openalex.org/W2094334619","https://openalex.org/W2143726250","https://openalex.org/W2226143795","https://openalex.org/W2293032062","https://openalex.org/W2475848550","https://openalex.org/W2770275887"],"related_works":["https://openalex.org/W2770275887","https://openalex.org/W1989002197","https://openalex.org/W4292937602","https://openalex.org/W3184903862","https://openalex.org/W2080586954","https://openalex.org/W2064808253","https://openalex.org/W2788380035","https://openalex.org/W2147783583","https://openalex.org/W2100975400","https://openalex.org/W2051296345"],"abstract_inverted_index":{"The":[0],"rapid":[1],"growth":[2],"of":[3,27,168],"research":[4],"focusing":[5],"on":[6],"RNA,":[7],"especially":[8],"for":[9,17,38,47,122,126],"RNA":[10,92],"interference":[11],"applications,":[12],"has":[13],"created":[14],"a":[15,18,36,51,62,80,178],"need":[16],"robust":[19],"method":[20,46,54,84,100],"that":[21,85,159],"can":[22,86,162],"accurately":[23],"determine":[24,56],"the":[25,43,107,118,123,139,165,169,182],"concentration":[26],"long":[28],"dsRNA.":[29],"As":[30],"it":[31],"is":[32,49,59],"difficult":[33],"to":[34,55,61,95,103,116,128,145,157],"find":[35,146],"source":[37],"pure":[39],"dsRNA":[40,127],"reference":[41],"material,":[42],"most":[44],"common":[45],"quantitation":[48],"using":[50,69],"reversed-phase":[52,98],"HPLC":[53],"purity,":[57],"which":[58],"linked":[60],"calibration":[63],"curve":[64],"prepared":[65],"by":[66],"measurements":[67],"obtained":[68],"UV":[70],"absorbance":[71],"at":[72],"260":[73],"nm.":[74],"In":[75],"this":[76,111,153],"study":[77,154],"we":[78,113,142,176],"developed":[79],"nucleic":[81],"acid":[82],"digestion":[83],"digest":[87],"both":[88],"double-":[89],"and":[90,93,105],"single-stranded":[91],"DNA":[94],"nucleosides.":[96,109],"A":[97],"HPLC/UV":[99],"was":[101],"used":[102],"separate":[104],"quantitate":[106],"monomeric":[108],"Using":[110],"method,":[112],"were":[114,143],"able":[115,144],"calculate":[117],"absorptivity":[119],"coefficient":[120,167],"(proxy":[121],"extinction":[124,166,184],"coefficient)":[125],"be":[129],"45.9":[130],"\u00b1":[131],"0.52":[132],"\u03bcg":[133],"mL-1/A260.":[134],"This":[135],"value":[136],"agrees":[137],"with":[138,173],"one":[140],"report":[141],"but":[147],"uses":[148],"an":[149],"orthogonal":[150],"method.":[151],"Moreover,":[152],"allowed":[155],"us":[156],"understand":[158],"sequence":[160],"design":[161],"dramatically":[163],"change":[164],"molecule.":[170],"For":[171],"molecules":[172],"ssRNA":[174],"overhangs,":[175],"observed":[177],"5%":[179],"reduction":[180],"in":[181],"calculated":[183],"coefficient.":[185]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2020-12-21T00:00:00"}