{"id":"https://openalex.org/W3095730183","doi":"https://doi.org/10.1161/res.127.suppl_1.475","title":"Abstract 475: Beta-adrenoceptor Stimulation Uniquely Regulates Exosome Micro-rna Content Secreted From Cardiomyocytes in vitro and Those Found in vivo Circulating in Blood","display_name":"Abstract 475: Beta-adrenoceptor Stimulation Uniquely Regulates Exosome Micro-rna Content Secreted From Cardiomyocytes in vitro and Those Found in vivo Circulating in Blood","publication_year":2020,"publication_date":"2020-07-31","ids":{"openalex":"https://openalex.org/W3095730183","doi":"https://doi.org/10.1161/res.127.suppl_1.475","mag":"3095730183"},"language":"en","primary_location":{"id":"doi:10.1161/res.127.suppl_1.475","doi":"https://doi.org/10.1161/res.127.suppl_1.475","is_oa":false,"landing_page_url":"https://doi.org/10.1161/res.127.suppl_1.475","pdf_url":null,"source":{"id":"https://openalex.org/S10652568","display_name":"Circulation Research","issn_l":"0009-7330","issn":["0009-7330","1524-4571"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112236069","display_name":"Jin Sook Kwon","orcid":"https://orcid.org/0009-0006-6513-9953","observed_orcids":["https://orcid.org/0009-0006-6513-9953"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jin Sook Kwon","raw_affiliation_strings":["Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","Jin Sook Kwon Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"Jin Sook Kwon Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044556689","display_name":"Erhe Gao","orcid":"https://orcid.org/0000-0002-5151-7857","observed_orcids":["https://orcid.org/0000-0002-5151-7857"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Erhe Gao","raw_affiliation_strings":["Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","Erhe Gao Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"Erhe Gao Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023999167","display_name":"Rajika Roy","orcid":"https://orcid.org/0000-0001-9394-3224","observed_orcids":["https://orcid.org/0000-0001-9394-3224"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rajika Roy","raw_affiliation_strings":["Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","Rajika Roy Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"Rajika Roy Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062462804","display_name":"Jessica Ibetti","orcid":"https://orcid.org/0000-0001-5237-6489","observed_orcids":["https://orcid.org/0000-0001-5237-6489"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"jessica Ibetti","raw_affiliation_strings":["Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","jessica Ibetti Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"jessica Ibetti Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003340760","display_name":"J. Kurt Chuprun","orcid":"https://orcid.org/0000-0003-1049-6890","observed_orcids":["https://orcid.org/0000-0003-1049-6890"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Kurt Chuprun","raw_affiliation_strings":["Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","J Kurt Chuprun Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"J Kurt Chuprun Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075648082","display_name":"Walter J. Koch","orcid":"https://orcid.org/0000-0002-8522-530X","observed_orcids":["https://orcid.org/0000-0002-8522-530X","https://orcid.org/0000-0002-0609-8649"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Walter J Koch","raw_affiliation_strings":["Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","Walter J Koch Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"Walter J Koch Lewis Katz Sch of Med at Temple Univ, Philadelphia, PA","institution_ids":["https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I84392919"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"127","issue":"Suppl_1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-adrenergic-signaling","display_name":"beta-adrenergic signaling","score":0.8510000109672546},{"id":"https://openalex.org/keywords/exosomes","display_name":"exosomes","score":0.9279999732971191},{"id":"https://openalex.org/keywords/microrna","display_name":"microRNA","score":0.8190000057220459},{"id":"https://openalex.org/keywords/cardiomyocytes","display_name":"cardiomyocytes","score":0.9660000205039978},{"id":"https://openalex.org/keywords/isoproterenol","display_name":"isoproterenol","score":0.9950000047683716},{"id":"https://openalex.org/keywords/heart-failure","display_name":"heart failure","score":0.8960000276565552},{"id":"https://openalex.org/keywords/circulating-exosomes","display_name":"circulating exosomes","score":0.8650000095367432},{"id":"https://openalex.org/keywords/mir-21","display_name":"miR-21","score":0.7559999823570251},{"id":"https://openalex.org/keywords/mir-489","display_name":"miR-489","score":0.8930000066757202},{"id":"https://openalex.org/keywords/mir-214","display_name":"miR-214","score":0.7670000195503235},{"id":"https://openalex.org/keywords/catecholamines","display_name":"catecholamines","score":0.8550000190734863},{"id":"https://openalex.org/keywords/exosome-size","display_name":"exosome size","score":0.5199999809265137},{"id":"https://openalex.org/keywords/neonatal-rat-ventricular-myocytes","display_name":"neonatal rat ventricular myocytes","score":0.800000011920929}],"concepts":[{"id":"https://openalex.org/C20518536","wikidata":"https://www.wikidata.org/wiki/Q6840469","display_name":"Microvesicles","level":4,"score":0.8622570037841797},{"id":"https://openalex.org/C2781261824","wikidata":"https://www.wikidata.org/wiki/Q903634","display_name":"Exosome","level":5,"score":0.7742168307304382},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.6555371284484863},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6413065195083618},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.6043122410774231},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.5394147038459778},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5346858501434326},{"id":"https://openalex.org/C2778921608","wikidata":"https://www.wikidata.org/wiki/Q2002035","display_name":"Ventricle","level":2,"score":0.5017061233520508},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.47961485385894775},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.45645686984062195},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4310971796512604},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.4282292127609253},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.42178580164909363},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3914647698402405},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37496212124824524},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.3567882180213928},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3004959523677826},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2681885361671448},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10713520646095276},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10362717509269714},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1161/res.127.suppl_1.475","doi":"https://doi.org/10.1161/res.127.suppl_1.475","is_oa":false,"landing_page_url":"https://doi.org/10.1161/res.127.suppl_1.475","pdf_url":null,"source":{"id":"https://openalex.org/S10652568","display_name":"Circulation Research","issn_l":"0009-7330","issn":["0009-7330","1524-4571"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2980453412","https://openalex.org/W3160617900","https://openalex.org/W3215741591","https://openalex.org/W2315861656","https://openalex.org/W3175286368","https://openalex.org/W2432047902","https://openalex.org/W2917270161","https://openalex.org/W2099039944","https://openalex.org/W4229698434","https://openalex.org/W2258037686"],"abstract_inverted_index":{"Background":[0],"and":[1,19,66,76,84,95,109,149,153,159,164,183,196,212,221,234,237,251,260,268,281,286,304,316,320,332,336,345,357,376],"Purpose:":[2],"The":[3,181],"influence":[4],"of":[5,12,42,80,88,97,117,185,202,223,279],"\u03b2-adrenoceptor":[6],"(\u03b2AR)":[7],"signaling":[8],"on":[9,60],"the":[10,40,73,114,155,203,219,292],"regulation":[11],"exosomes":[13,45,152,186,233],"secreted":[14,81,110],"from":[15,106,124,188,306,350],"cardiomyocytes":[16],"is":[17,29],"unknown":[18],"since":[20],"catecholamines":[21],"are":[22],"increased":[23],"in":[24,31,39,46,67,69,151,154,207,218,230,241,246,264,312,314,317,334,359,374],"heart":[25,235,242],"failure":[26],"(HF),":[27],"there":[28],"interest":[30],"uncovering":[32],"whether":[33,54],"\u03b2ARs":[34],"can":[35,71],"induce":[36],"specific":[37],"changes":[38,333,348],"content":[41,79],"circulating":[43,189,360],"blood":[44,190,220,232,247,273,319,335,361],"HF.":[47],"In":[48,257,272],"this":[49,309],"study,":[50],"we":[51,112,275],"have":[52],"evaluated":[53,113,200],"\u03b2AR":[55,293],"stimulation":[56],"by":[57],"isoproterenol":[58],"(ISO)":[59],"neonatal":[61],"rat":[62],"ventricle":[63],"myocytes":[64,108,156,208,313,337],"(NRVMs)":[65],"vivo":[68,318],"mice":[70,176],"alter":[72],"number,":[74],"size":[75,96,184,303],"microRNA":[77],"(miR)":[78],"exosomes.":[82,362],"Methods":[83],"Results:":[85],"ISO":[86,131,169,255,295,326],"treatment":[87,132],"NRVMs":[89],"did":[90,227],"not":[91,192,228,245,284,301,339],"change":[92,229],"exosome":[93,302],"number":[94,182,305],"compared":[98],"than":[99],"vehicle":[100],"(PBS).":[101],"After":[102],"purifying":[103],"total":[104],"RNA":[105],"treated":[107,324],"exosomes,":[111,274],"expression":[115,201],"level":[116],"37":[118],"candidate":[119],"miR\u2019s,":[120],"which":[121],"were":[122,161,323,330,338],"selected":[123],"previous":[125],"microarray":[126],"data.":[127],"We":[128,199,289],"found":[129,276,290,311,331],"that":[130,291,322],"decreased":[133],"14":[134,204],"miR\u2019s":[135],"(miR-222,":[136],"-106a,":[137],"-292a,":[138],"-181b,":[139],"-210,":[140],"-489,":[141],"-214,":[142],"-1947,":[143],"-195,":[144],"-17,":[145],"-7b,":[146],"-93,":[147],"-532":[148],"-19a)":[150],"themselves,":[157],"mir-292a":[158],"-1947":[160],"up":[162,270],"regulated":[163],"mir-7b,":[165],"down":[166],"regulated.":[167,271],"Further,":[168],"was":[170,191,239,262,310],"then":[171],"used":[172],"to":[173],"treat":[174],"C57":[175],"via":[177],"osmotic":[178],"pump":[179],"chronically.":[180],"purified":[187],"changed":[193],"after":[194,254],"2":[195,250],"8":[197,252],"weeks.":[198],"miRs":[205,365],"down-regulated":[206,263],"as":[209],"well":[210],"miR-1":[211],"-21":[213],"(important":[214],"cardiac":[215],"miRs)":[216],"both":[217,231,248,347],"hearts":[222,265],"ISO-treated":[224],"mice.":[225],"MiR-1":[226],"tissue":[236,243],"miR-21":[238],"up-regulated":[240],"but":[244,283,300,327],"at":[249],"weeks":[253],"treatment.":[256],"addition,":[258],"miRNA-489":[259],"-7b":[261,282],"with":[266,325],"miR-214":[267,285],"-292a":[269],"only":[277],"down-regulation":[278],"miR-489":[280],"292a.":[287],"Conclusions:":[288],"agonist":[294],"altered":[296],"exosomal":[297],"miR":[298],"contents":[299],"myocytes.":[307],"Importantly,":[308],"culture":[315],"myocardium":[321],"several":[328],"differences":[329],"homogeneous.":[340],"However,":[341],"two":[342],"miRs,":[343],"mir-7b":[344],"-miR-489,":[346],"similarly":[349],"their":[351,377],"origin":[352],"(NRVMs":[353],"or":[354],"mouse":[355],"hearts)":[356],"also":[358],"Therefore,":[363],"these":[364],"may":[366],"represent":[367],"biomarkers":[368],"for":[369],"sympathetic":[370],"nervous":[371],"system":[372],"abnormalities":[373],"HF":[375],"therapeutic":[378],"potential":[379],"should":[380],"be":[381],"evaluated.":[382]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2020-11-09T00:00:00"}