{"id":"https://openalex.org/W3095628124","doi":"https://doi.org/10.1017/s1431927620024630","title":"FE-SEM Characterization of \u03b1-Mannose Density and Surface Mapping Changes in Human Sperm Head During In Vitro Capacitation","display_name":"FE-SEM Characterization of \u03b1-Mannose Density and Surface Mapping Changes in Human Sperm Head During In Vitro Capacitation","publication_year":2020,"publication_date":"2020-10-30","ids":{"openalex":"https://openalex.org/W3095628124","doi":"https://doi.org/10.1017/s1431927620024630","mag":"3095628124","pmid":"https://pubmed.ncbi.nlm.nih.gov/33121558"},"language":"en","primary_location":{"id":"doi:10.1017/s1431927620024630","doi":"https://doi.org/10.1017/s1431927620024630","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s1431927620024630","pdf_url":null,"source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021120907","display_name":"Mar\u00eda Jos\u00e9 G\u00f3mez\u2010Torres","orcid":"https://orcid.org/0000-0003-2384-8154","observed_orcids":["https://orcid.org/0000-0003-2384-8154"]},"institutions":[{"id":"https://openalex.org/I130194489","display_name":"University of Alicante","ror":"https://ror.org/05t8bcz72","country_code":"ES","type":"education","lineage":["https://openalex.org/I130194489"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"Mar\u00eda Jos\u00e9 G\u00f3mez-Torres","raw_affiliation_strings":["C\u00e1tedra Human Fertility, Universidad de Alicante, Alicante, Spain","Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain"],"raw_orcid":"https://orcid.org/0000-0003-2384-8154","affiliations":[{"raw_affiliation_string":"C\u00e1tedra Human Fertility, Universidad de Alicante, Alicante, Spain","institution_ids":["https://openalex.org/I130194489"]},{"raw_affiliation_string":"Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain","institution_ids":["https://openalex.org/I130194489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082130196","display_name":"Laura Robles\u2010G\u00f3mez","orcid":"https://orcid.org/0000-0001-8000-3671","observed_orcids":["https://orcid.org/0000-0001-8000-3671"]},"institutions":[{"id":"https://openalex.org/I130194489","display_name":"University of Alicante","ror":"https://ror.org/05t8bcz72","country_code":"ES","type":"education","lineage":["https://openalex.org/I130194489"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Laura Robles-G\u00f3mez","raw_affiliation_strings":["Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain"],"raw_orcid":"https://orcid.org/0000-0001-8000-3671","affiliations":[{"raw_affiliation_string":"Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain","institution_ids":["https://openalex.org/I130194489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025453198","display_name":"Natalia Huerta\u2010Retamal","orcid":"https://orcid.org/0000-0002-1553-6794","observed_orcids":["https://orcid.org/0000-0002-1553-6794"]},"institutions":[{"id":"https://openalex.org/I130194489","display_name":"University of Alicante","ror":"https://ror.org/05t8bcz72","country_code":"ES","type":"education","lineage":["https://openalex.org/I130194489"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Natalia Huerta-Retamal","raw_affiliation_strings":["Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain"],"raw_orcid":"https://orcid.org/0000-0002-1553-6794","affiliations":[{"raw_affiliation_string":"Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain","institution_ids":["https://openalex.org/I130194489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023134313","display_name":"Paula S\u00e1ez\u2010Espinosa","orcid":"https://orcid.org/0000-0002-6840-8788","observed_orcids":["https://orcid.org/0000-0002-6840-8788"]},"institutions":[{"id":"https://openalex.org/I130194489","display_name":"University of Alicante","ror":"https://ror.org/05t8bcz72","country_code":"ES","type":"education","lineage":["https://openalex.org/I130194489"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Paula S\u00e1ez-Espinosa","raw_affiliation_strings":["Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain"],"raw_orcid":"https://orcid.org/0000-0002-6840-8788","affiliations":[{"raw_affiliation_string":"Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain","institution_ids":["https://openalex.org/I130194489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032691788","display_name":"Manuel Avil\u00e9s","orcid":"https://orcid.org/0000-0003-4396-4718","observed_orcids":["https://orcid.org/0000-0003-4396-4718"]},"institutions":[{"id":"https://openalex.org/I4210146619","display_name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria","ror":"https://ror.org/053j10c72","country_code":"ES","type":"facility","lineage":["https://openalex.org/I4210146619"]},{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Manuel Avil\u00e9s","raw_affiliation_strings":["Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Medicina, Universidad de Murcia e IMIB-Arrixaca, Murcia, Spain"],"raw_orcid":"https://orcid.org/0000-0003-4396-4718","affiliations":[{"raw_affiliation_string":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Medicina, Universidad de Murcia e IMIB-Arrixaca, Murcia, Spain","institution_ids":["https://openalex.org/I4210146619","https://openalex.org/I80180929"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041990242","display_name":"Jon Aizpur\u00faa","orcid":"https://orcid.org/0000-0002-0801-3960","observed_orcids":["https://orcid.org/0000-0002-0801-3960"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jon Aizpurua","raw_affiliation_strings":["IVF Spain, Reproductive Medicine, Alicante, Spain"],"raw_orcid":"https://orcid.org/0000-0002-0801-3960","affiliations":[{"raw_affiliation_string":"IVF Spain, Reproductive Medicine, Alicante, Spain","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028699632","display_name":"Alejandro Romero","orcid":"https://orcid.org/0000-0002-5743-0613","observed_orcids":["https://orcid.org/0000-0002-5743-0613"]},"institutions":[{"id":"https://openalex.org/I130194489","display_name":"University of Alicante","ror":"https://ror.org/05t8bcz72","country_code":"ES","type":"education","lineage":["https://openalex.org/I130194489"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Alejandro Romero","raw_affiliation_strings":["Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain"],"raw_orcid":"https://orcid.org/0000-0002-5743-0613","affiliations":[{"raw_affiliation_string":"Departamento de Biotecnolog\u00eda, Facultad de Ciencias, Universidad de Alicante, Ap. C. 99, Alicante03080, Spain","institution_ids":["https://openalex.org/I130194489"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5021120907"],"corresponding_institution_ids":["https://openalex.org/I130194489"],"apc_list":{"value":2980,"currency":"USD","value_usd":2980},"apc_paid":null,"fwci":0.6714,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.62732765,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"26","issue":"6","first_page":"1220","last_page":"1225"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sperm-capacitation","display_name":"sperm capacitation","score":0.9290000200271606},{"id":"https://openalex.org/keywords/alpha-mannose","display_name":"alpha-mannose","score":0.871999979019165},{"id":"https://openalex.org/keywords/acrosome","display_name":"acrosome","score":0.9129999876022339},{"id":"https://openalex.org/keywords/plasma-membrane","display_name":"plasma membrane","score":0.5640000104904175}],"concepts":[{"id":"https://openalex.org/C2780301668","wikidata":"https://www.wikidata.org/wiki/Q1728362","display_name":"Capacitation","level":3,"score":0.9614574909210205},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.6251078248023987},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.5903571844100952},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.581937313079834},{"id":"https://openalex.org/C2780312720","wikidata":"https://www.wikidata.org/wiki/Q5689100","display_name":"Head (geology)","level":2,"score":0.5085726976394653},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.4703526496887207},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3497140407562256},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3216460347175598},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.20669615268707275},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.1950218379497528},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.15126970410346985},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14298492670059204},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.07618889212608337},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D006046","descriptor_name":"Gold","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008358","descriptor_name":"Mannose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008855","descriptor_name":"Microscopy, Electron, Scanning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013077","descriptor_name":"Sperm Head","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053768","descriptor_name":"Metal Nanoparticles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1017/s1431927620024630","doi":"https://doi.org/10.1017/s1431927620024630","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s1431927620024630","pdf_url":null,"source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"},{"id":"pmid:33121558","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33121558","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada","raw_type":"Journal Article"},{"id":"pmh:oai:rua.ua.es:10045/110911","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10045/110911","pdf_url":null,"source":{"id":"https://openalex.org/S4306400416","display_name":"Repositorio Institucional de la Universidad de Alicante (Universidad de Alicante)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I130194489","host_organization_name":"University of Alicante","host_organization_lineage":["https://openalex.org/I130194489"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W5914777","https://openalex.org/W1263610036","https://openalex.org/W1512329124","https://openalex.org/W1796017043","https://openalex.org/W1870327319","https://openalex.org/W1968290970","https://openalex.org/W1979866830","https://openalex.org/W1993235105","https://openalex.org/W2008082064","https://openalex.org/W2013599693","https://openalex.org/W2015550884","https://openalex.org/W2026628433","https://openalex.org/W2052046062","https://openalex.org/W2060311782","https://openalex.org/W2065930105","https://openalex.org/W2084857735","https://openalex.org/W2087119080","https://openalex.org/W2090254194","https://openalex.org/W2105204151","https://openalex.org/W2123875939","https://openalex.org/W2124043500","https://openalex.org/W2124758992","https://openalex.org/W2125679477","https://openalex.org/W2132072109","https://openalex.org/W2136627330","https://openalex.org/W2144558876","https://openalex.org/W2150077769","https://openalex.org/W2154475163","https://openalex.org/W2162033352","https://openalex.org/W2170374634","https://openalex.org/W2511119393","https://openalex.org/W2581327015","https://openalex.org/W2787051988","https://openalex.org/W2904994692","https://openalex.org/W2980901582"],"related_works":["https://openalex.org/W3209088754","https://openalex.org/W3121471229","https://openalex.org/W1916320359","https://openalex.org/W2300419021","https://openalex.org/W2046647871","https://openalex.org/W1979866830","https://openalex.org/W2418774644","https://openalex.org/W2087574630","https://openalex.org/W1964022939","https://openalex.org/W3153857114"],"abstract_inverted_index":{"Sperm":[0],"capacitation":[1,24,48,78,121,164],"includes":[2],"the":[3,10,14,51,65,98],"reorganization":[4],"of":[5,13,113,128],"plasma":[6],"membrane":[7,70],"components":[8],"and":[9,19,54,68,74,125,150,160],"outstanding":[11],"modification":[12],"glycocalyx.":[15],"The":[16],"\u03b1-mannose":[17,52,66,92,114],"presence":[18],"location":[20],"during":[21,163],"in":[22,29,46,76,119],"vitro":[23,47,77,120],"have":[25],"been":[26],"commonly":[27],"described":[28],"human":[30,157],"spermatozoa":[31],"using":[32,81,148],"Concanavalin":[33],"A":[34],"(Con":[35],"A)":[36],"lectin.":[37],"However,":[38],"it":[39],"is":[40],"still":[41],"unclear":[42],"to":[43],"date":[44],"how":[45],"time":[49],"affects":[50],"residues":[53,93,130],"their":[55,134],"topographic":[56],"spatial":[57],"distribution":[58],"on":[59,97],"sperm":[60,106,158],"membrane.":[61],"Here,":[62],"we":[63],"characterized":[64],"density":[67],"specific":[69],"domain":[71,139],"locations":[72],"before":[73],"after":[75],"(1\u20134":[79],"h)":[80],"high-resolution":[82],"field":[83],"emission":[84],"scanning":[85],"electron":[86],"microscopy":[87],"(FE-SEM).":[88],"Results":[89],"showed":[90],"that":[91,145],"were":[94],"present":[95],"preferably":[96],"acrosome":[99],"domains":[100],"for":[101],"all":[102],"physiological":[103],"conditions.":[104],"Uncapacitated":[105],"comparatively":[107],"exhibits":[108],"significant":[109,124],"highest":[110],"labeling":[111],"densities":[112],"residues.":[115],"In":[116],"addition,":[117],"as":[118],"takes":[122],"place,":[123],"progressive":[126],"decreasing":[127],"sugar":[129],"was":[131],"combined":[132,146],"with":[133],"relocation":[135],"mostly":[136],"affecting":[137],"acrosomal":[138],"apical":[140],"areas.":[141],"Our":[142],"findings":[143],"reveal":[144],"approach":[147],"FE-SEM":[149],"gold":[151],"nanoparticle":[152],"topographical":[153],"mapping":[154],"offers":[155],"new":[156],"biomolecular":[159],"structural":[161],"details":[162],"events.":[165]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2020-11-09T00:00:00"}