{"id":"https://openalex.org/W3093879893","doi":"https://doi.org/10.1093/intbio/zyaa021","title":"Co-cultivation of microbial sub-communities in microfluidic droplets facilitates high-resolution genomic dissection of microbial \u2018dark matter\u2019","display_name":"Co-cultivation of microbial sub-communities in microfluidic droplets facilitates high-resolution genomic dissection of microbial \u2018dark matter\u2019","publication_year":2020,"publication_date":"2020-10-22","ids":{"openalex":"https://openalex.org/W3093879893","doi":"https://doi.org/10.1093/intbio/zyaa021","mag":"3093879893","pmid":"https://pubmed.ncbi.nlm.nih.gov/33089329","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7671993"},"language":"en","primary_location":{"id":"doi:10.1093/intbio/zyaa021","doi":"https://doi.org/10.1093/intbio/zyaa021","is_oa":false,"landing_page_url":"https://doi.org/10.1093/intbio/zyaa021","pdf_url":null,"source":{"id":"https://openalex.org/S10577112","display_name":"Integrative Biology","issn_l":"1757-9694","issn":["1757-9694","1757-9708"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Integrative Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7671993","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5008401166","display_name":"James Tan","orcid":"https://orcid.org/0000-0002-0550-932X","observed_orcids":["https://orcid.org/0000-0002-0550-932X"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James Y Tan","raw_affiliation_strings":["Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA"],"raw_orcid":"https://orcid.org/0000-0002-0550-932X","affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Sida Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sida Wang","raw_affiliation_strings":["Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075036643","display_name":"Gregory J. Dick","orcid":"https://orcid.org/0000-0001-7666-6288","observed_orcids":["https://orcid.org/0000-0001-7666-6288"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gregory J Dick","raw_affiliation_strings":["Department of Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI, USA","Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049294221","display_name":"Vincent Bensan Young","orcid":"https://orcid.org/0000-0003-3687-2364","observed_orcids":["https://orcid.org/0000-0003-3687-2364"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vincent B Young","raw_affiliation_strings":["Deparment of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA","Infectious Diseases Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Deparment of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Infectious Diseases Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020610727","display_name":"David H. Sherman","orcid":"https://orcid.org/0000-0001-8334-3647","observed_orcids":["https://orcid.org/0000-0001-8334-3647"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David H Sherman","raw_affiliation_strings":["Deparment of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA","Department of Chemistry, University of Michigan, Ann Arbor, MI, USA","Department of Medicinal Chemistry, University of Michigan, Ann Arbor, MI, USA","Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Deparment of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Department of Chemistry, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Department of Medicinal Chemistry, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091616917","display_name":"Mark A. Burns","orcid":"https://orcid.org/0000-0001-7384-4165","observed_orcids":["https://orcid.org/0000-0001-7384-4165"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark A Burns","raw_affiliation_strings":["Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA","Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101946266","display_name":"Xiaoxia Nina Lin","orcid":"https://orcid.org/0000-0003-4470-4432","observed_orcids":["https://orcid.org/0000-0003-4470-4432"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Xiaoxia N Lin","raw_affiliation_strings":["Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA","Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5101946266"],"corresponding_institution_ids":["https://openalex.org/I27837315"],"apc_list":{"value":3522,"currency":"USD","value_usd":3522},"apc_paid":null,"fwci":1.1215,"has_fulltext":false,"cited_by_count":26,"citation_normalized_percentile":{"value":0.75640242,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":98},"biblio":{"volume":"12","issue":"11","first_page":"263","last_page":"274"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.6996999979019165,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.6996999979019165,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.09780000150203705,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11407","display_name":"Innovative Microfluidic and Catalytic Techniques Innovation","score":0.094200000166893,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microbial-dark-matter","display_name":"microbial dark matter","score":0.7889999747276306},{"id":"https://openalex.org/keywords/uncultured-bacteria","display_name":"uncultured bacteria","score":0.9200000166893005},{"id":"https://openalex.org/keywords/co-culture","display_name":"co-culture","score":0.8379999995231628},{"id":"https://openalex.org/keywords/microfluidic-droplets","display_name":"microfluidic droplets","score":0.9860000014305115},{"id":"https://openalex.org/keywords/droplet-encapsulation","display_name":"droplet encapsulation","score":0.7059999704360962},{"id":"https://openalex.org/keywords/gut-microbiota","display_name":"gut microbiota","score":0.7680000066757202},{"id":"https://openalex.org/keywords/fecal-microbiota","display_name":"fecal microbiota","score":0.7570000290870667},{"id":"https://openalex.org/keywords/metagenome-assembled-genomes","display_name":"metagenome-assembled genomes","score":0.9580000042915344},{"id":"https://openalex.org/keywords/whole-genome-amplification","display_name":"whole genome amplification","score":0.8769999742507935},{"id":"https://openalex.org/keywords/neisseriaceae","display_name":"Neisseriaceae","score":0.9430000185966492},{"id":"https://openalex.org/keywords/fatty-acid-degradation","display_name":"fatty acid degradation","score":0.9639999866485596},{"id":"https://openalex.org/keywords/rare-bacterial-taxa","display_name":"rare bacterial taxa","score":0.3709999918937683}],"concepts":[{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.938836932182312},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.6286845803260803},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6228431463241577},{"id":"https://openalex.org/C81407943","wikidata":"https://www.wikidata.org/wiki/Q17149178","display_name":"Microbial population biology","level":3,"score":0.586176335811615},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5373085737228394},{"id":"https://openalex.org/C8673954","wikidata":"https://www.wikidata.org/wiki/Q138845","display_name":"Microfluidics","level":2,"score":0.5118014216423035},{"id":"https://openalex.org/C186795189","wikidata":"https://www.wikidata.org/wiki/Q3631109","display_name":"Environmental biotechnology","level":4,"score":0.47235023975372314},{"id":"https://openalex.org/C3020199158","wikidata":"https://www.wikidata.org/wiki/Q210521","display_name":"High resolution","level":2,"score":0.43570220470428467},{"id":"https://openalex.org/C175605896","wikidata":"https://www.wikidata.org/wiki/Q39833","display_name":"Microorganism","level":3,"score":0.4293377995491028},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4249003529548645},{"id":"https://openalex.org/C101985253","wikidata":"https://www.wikidata.org/wiki/Q1073526","display_name":"Shotgun sequencing","level":4,"score":0.4236098527908325},{"id":"https://openalex.org/C3742359","wikidata":"https://www.wikidata.org/wiki/Q4839988","display_name":"Bacterial genome size","level":4,"score":0.4131776988506317},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.34157973527908325},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3382914364337921},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.30427175760269165},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.26354682445526123},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.0},{"id":"https://openalex.org/C62649853","wikidata":"https://www.wikidata.org/wiki/Q199687","display_name":"Remote sensing","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000069196","descriptor_name":"Gastrointestinal Microbiome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005810","descriptor_name":"Multigene Family","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008828","descriptor_name":"Microbiological Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018920","descriptor_name":"Coculture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044085","descriptor_name":"Microfluidics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064307","descriptor_name":"Microbiota","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1093/intbio/zyaa021","doi":"https://doi.org/10.1093/intbio/zyaa021","is_oa":false,"landing_page_url":"https://doi.org/10.1093/intbio/zyaa021","pdf_url":null,"source":{"id":"https://openalex.org/S10577112","display_name":"Integrative Biology","issn_l":"1757-9694","issn":["1757-9694","1757-9708"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Integrative Biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7671993","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7671993","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Integr Biol (Camb)","raw_type":"Text"},{"id":"pmid:33089329","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33089329","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Integrative biology : quantitative biosciences from nano to macro","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:7671993","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7671993","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Integr Biol (Camb)","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4000000059604645}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2710744775","display_name":"Metagenomic Dissection of the Gut Microbiota","funder_award_id":"R21HG005077","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G4569188361","display_name":"Cellular Biotechnology Training Program (CBTP)","funder_award_id":"T32GM008353","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G9000919722","display_name":"Cellular Biotechnology Training Program (CBTP) - Years 31-35","funder_award_id":"T32GM145304","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":76,"referenced_works":["https://openalex.org/W182634393","https://openalex.org/W1553914850","https://openalex.org/W1564294668","https://openalex.org/W1589587257","https://openalex.org/W1958058086","https://openalex.org/W1969182483","https://openalex.org/W1991497480","https://openalex.org/W1996047071","https://openalex.org/W2004548026","https://openalex.org/W2005249358","https://openalex.org/W2008054523","https://openalex.org/W2020398205","https://openalex.org/W2029886517","https://openalex.org/W2029945156","https://openalex.org/W2037015298","https://openalex.org/W2044482540","https://openalex.org/W2044638285","https://openalex.org/W2059092073","https://openalex.org/W2071841602","https://openalex.org/W2075220255","https://openalex.org/W2075480465","https://openalex.org/W2077072558","https://openalex.org/W2078599166","https://openalex.org/W2078779451","https://openalex.org/W2086524698","https://openalex.org/W2089444561","https://openalex.org/W2102681753","https://openalex.org/W2107772251","https://openalex.org/W2108718991","https://openalex.org/W2109937739","https://openalex.org/W2112364185","https://openalex.org/W2112738213","https://openalex.org/W2114695967","https://openalex.org/W2115944543","https://openalex.org/W2116913446","https://openalex.org/W2117457769","https://openalex.org/W2119343060","https://openalex.org/W2119882126","https://openalex.org/W2124451996","https://openalex.org/W2125484216","https://openalex.org/W2131026689","https://openalex.org/W2133537846","https://openalex.org/W2147790901","https://openalex.org/W2151350595","https://openalex.org/W2152093511","https://openalex.org/W2152907704","https://openalex.org/W2158630697","https://openalex.org/W2158784643","https://openalex.org/W2166358290","https://openalex.org/W2167033123","https://openalex.org/W2169332127","https://openalex.org/W2171777099","https://openalex.org/W2332336619","https://openalex.org/W2345813655","https://openalex.org/W2527684409","https://openalex.org/W2549670165","https://openalex.org/W2552041992","https://openalex.org/W2593203552","https://openalex.org/W2599417231","https://openalex.org/W2609317492","https://openalex.org/W2624106029","https://openalex.org/W2626264906","https://openalex.org/W2736105902","https://openalex.org/W2755336277","https://openalex.org/W2760023427","https://openalex.org/W2784164089","https://openalex.org/W2794647037","https://openalex.org/W2797240324","https://openalex.org/W2807727258","https://openalex.org/W2887220719","https://openalex.org/W2951160681","https://openalex.org/W2953098897","https://openalex.org/W2953167790","https://openalex.org/W2971439707","https://openalex.org/W2976566477","https://openalex.org/W3137074281"],"related_works":["https://openalex.org/W6906532097","https://openalex.org/W4406402974","https://openalex.org/W2944524871","https://openalex.org/W2059092073","https://openalex.org/W137034129","https://openalex.org/W7100503950","https://openalex.org/W6887902858","https://openalex.org/W2990089557","https://openalex.org/W2736259154","https://openalex.org/W4292181374"],"abstract_inverted_index":{"While":[0],"the":[1,5,13,66,72,90,127,131,145,163,173,185],"'unculturable'":[2],"majority":[3],"of":[4,27,48,61,68,76,98,119,140,144,159,187,193],"bacterial":[6],"world":[7],"is":[8],"accessible":[9],"with":[10,133,177],"culture-independent":[11],"tools,":[12],"inability":[14],"to":[15,45,111,129,172,183],"study":[16,184],"these":[17],"bacteria":[18],"using":[19],"culture-dependent":[20],"approaches":[21],"has":[22],"severely":[23],"limited":[24],"our":[25],"understanding":[26],"their":[28],"ecological":[29,55],"roles":[30],"and":[31,74,94,157,170],"interactions.":[32],"To":[33],"circumvent":[34],"cultivation":[35],"barriers,":[36],"we":[37,104],"utilize":[38],"microfluidic":[39],"droplets":[40],"as":[41],"localized,":[42],"nanoliter-size":[43],"bioreactors":[44],"co-cultivate":[46],"subsets":[47],"microbial":[49,174,194],"communities.":[50,195],"This":[51],"co-localization":[52],"can":[53,180],"support":[54],"interactions":[56,186],"between":[57],"a":[58,80,85],"reduced":[59],"number":[60],"encapsulated":[62],"cells.":[63],"We":[64],"demonstrated":[65,126,167],"utility":[67],"this":[69,107,178],"approach":[70,108],"in":[71,153,162],"encapsulation":[73],"co-cultivation":[75],"droplet":[77,124],"sub-communities":[78,100,132],"from":[79,84,101,122],"fecal":[81],"sample":[82],"collected":[83],"healthy":[86],"human":[87,164],"subject.":[88],"With":[89],"whole":[91],"genome":[92],"amplification":[93],"metagenomic":[95],"shotgun":[96],"sequencing":[97],"co-cultivated":[99],"22":[102],"droplets,":[103],"observed":[105],"that":[106],"provides":[109],"accessibility":[110,171],"uncharacterized":[112],"gut":[113],"commensals":[114],"for":[115],"study.":[116],"The":[117,166],"recovery":[118],"metagenome-assembled":[120],"genomes":[121],"one":[123,141],"sub-community":[125],"capability":[128],"dissect":[130],"high-genomic":[134],"resolution.":[135],"In":[136],"particular,":[137],"genomic":[138,168],"characterization":[139],"novel":[142],"member":[143],"family":[146],"Neisseriaceae":[147],"revealed":[148],"implications":[149],"regarding":[150],"its":[151],"participation":[152],"fatty":[154],"acid":[155],"degradation":[156],"production":[158],"atherogenic":[160],"intermediates":[161],"gut.":[165],"resolution":[169],"'dark":[175],"matter'":[176],"methodology":[179],"be":[181],"applied":[182],"rare":[188],"or":[189],"previously":[190],"uncultivated":[191],"members":[192]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2020-10-29T00:00:00"}