{"id":"https://openalex.org/W3091908850","doi":"https://doi.org/10.1038/s41598-020-73813-7","title":"Thermostability of a recombinant G protein-coupled receptor expressed at high level in mammalian cell culture","display_name":"Thermostability of a recombinant G protein-coupled receptor expressed at high level in mammalian cell culture","publication_year":2020,"publication_date":"2020-10-08","ids":{"openalex":"https://openalex.org/W3091908850","doi":"https://doi.org/10.1038/s41598-020-73813-7","mag":"3091908850","pmid":"https://pubmed.ncbi.nlm.nih.gov/33033368","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7546613"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-020-73813-7","doi":"https://doi.org/10.1038/s41598-020-73813-7","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-73813-7","pdf_url":"https://www.nature.com/articles/s41598-020-73813-7.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-020-73813-7.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049076141","display_name":"Alexei Yeliseev","orcid":"https://orcid.org/0000-0003-4131-0913","observed_orcids":["https://orcid.org/0000-0003-4131-0913"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210121815","display_name":"National Institute on Alcohol Abuse and Alcoholism","ror":"https://ror.org/02jzrsm59","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Alexei Yeliseev","raw_affiliation_strings":["National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA. yeliseeva@mail.nih.gov","National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA. yeliseeva@mail.nih.gov","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210121815"]},{"raw_affiliation_string":"National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Arjen van den Berg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Arjen van den Berg","raw_affiliation_strings":["ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA","institution_ids":["https://openalex.org/I4210142480"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009134037","display_name":"Lioudmila Zoubak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210121815","display_name":"National Institute on Alcohol Abuse and Alcoholism","ror":"https://ror.org/02jzrsm59","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lioudmila Zoubak","raw_affiliation_strings":["National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008995549","display_name":"Kirk G. Hines","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210121815","display_name":"National Institute on Alcohol Abuse and Alcoholism","ror":"https://ror.org/02jzrsm59","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kirk Hines","raw_affiliation_strings":["National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020967531","display_name":"Sam Stepnowski","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sam Stepnowski","raw_affiliation_strings":["ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA","institution_ids":["https://openalex.org/I4210142480"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Kyle Williston","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kyle Williston","raw_affiliation_strings":["ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA","institution_ids":["https://openalex.org/I4210142480"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Wanhua Yan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wanhua Yan","raw_affiliation_strings":["ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA","institution_ids":["https://openalex.org/I4210142480"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052880769","display_name":"Klaus Gawrisch","orcid":"https://orcid.org/0000-0002-0841-1539","observed_orcids":["https://orcid.org/0000-0002-0841-1539"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210121815","display_name":"National Institute on Alcohol Abuse and Alcoholism","ror":"https://ror.org/02jzrsm59","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Klaus Gawrisch","raw_affiliation_strings":["National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute on Alcoholism and Alcohol Abuse, NIH, Bethesda, MD, 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210121815"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113919033","display_name":"Jonathan F. Zmuda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jonathan Zmuda","raw_affiliation_strings":["ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ThermoFisher Scientific, 7335 Executive Way, Frederick, MD, 21704, USA","institution_ids":["https://openalex.org/I4210142480"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5049076141"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210121815"],"apc_list":{"value":1870,"currency":"USD","value_usd":1870},"apc_paid":{"value":1870,"currency":"USD","value_usd":1870},"fwci":0.8103,"has_fulltext":true,"cited_by_count":14,"citation_normalized_percentile":{"value":0.68003173,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"10","issue":"1","first_page":"16805","last_page":"16805"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.9459999799728394},{"id":"https://openalex.org/keywords/cb2-receptor","display_name":"CB2 receptor","score":0.9639999866485596},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.9369999766349792},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7649999856948853},{"id":"https://openalex.org/keywords/mammalian-cell-culture","display_name":"mammalian cell culture","score":0.8399999737739563},{"id":"https://openalex.org/keywords/detergent-solubilization","display_name":"detergent solubilization","score":0.8920000195503235},{"id":"https://openalex.org/keywords/n-terminal-glycosylation","display_name":"N-terminal glycosylation","score":0.8640000224113464},{"id":"https://openalex.org/keywords/protein-palmitoylation","display_name":"palmitoylation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/isotopic-labeling","display_name":"isotopic labeling","score":0.7129999995231628},{"id":"https://openalex.org/keywords/nuclear-magnetic-resonance","display_name":"nuclear magnetic resonance","score":0.6010000109672546},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.4090000092983246}],"concepts":[{"id":"https://openalex.org/C135285700","wikidata":"https://www.wikidata.org/wiki/Q38173","display_name":"G protein-coupled receptor","level":3,"score":0.8304179906845093},{"id":"https://openalex.org/C161200384","wikidata":"https://www.wikidata.org/wiki/Q1757382","display_name":"Integral membrane protein","level":4,"score":0.642376184463501},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.5776042342185974},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.575690507888794},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5033654570579529},{"id":"https://openalex.org/C2781216036","wikidata":"https://www.wikidata.org/wiki/Q902494","display_name":"Thermostability","level":3,"score":0.465831458568573},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4461445212364197},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.4420836567878723},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.42208772897720337},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3832210600376129},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37796568870544434},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.34635478258132935},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11097100377082825},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011499","descriptor_name":"Protein Processing, Post-Translational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D043562","descriptor_name":"Receptors, G-Protein-Coupled","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D043562","descriptor_name":"Receptors, G-Protein-Coupled","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D043885","descriptor_name":"Receptor, Cannabinoid, CB2","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D043885","descriptor_name":"Receptor, Cannabinoid, CB2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D055550","descriptor_name":"Protein Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1038/s41598-020-73813-7","doi":"https://doi.org/10.1038/s41598-020-73813-7","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-73813-7","pdf_url":"https://www.nature.com/articles/s41598-020-73813-7.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7546613","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7546613","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:33033368","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33033368","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1038/s41598-020-73813-7","doi":"https://doi.org/10.1038/s41598-020-73813-7","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-73813-7","pdf_url":"https://www.nature.com/articles/s41598-020-73813-7.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337330","display_name":"National Institute on Alcohol Abuse and Alcoholism","ror":"https://ror.org/02jzrsm59"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3091908850.pdf","grobid_xml":"https://content.openalex.org/works/W3091908850.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W1505054029","https://openalex.org/W1574857337","https://openalex.org/W1908561802","https://openalex.org/W1968533552","https://openalex.org/W1974381616","https://openalex.org/W1975740938","https://openalex.org/W1976360899","https://openalex.org/W1979728801","https://openalex.org/W1980826665","https://openalex.org/W1981058524","https://openalex.org/W1981687442","https://openalex.org/W1985571627","https://openalex.org/W1985581279","https://openalex.org/W1989424463","https://openalex.org/W1993787660","https://openalex.org/W2000758567","https://openalex.org/W2002396116","https://openalex.org/W2023366312","https://openalex.org/W2029891166","https://openalex.org/W2030975239","https://openalex.org/W2033032481","https://openalex.org/W2036941089","https://openalex.org/W2042142307","https://openalex.org/W2050784306","https://openalex.org/W2053278081","https://openalex.org/W2063233717","https://openalex.org/W2065385991","https://openalex.org/W2070458501","https://openalex.org/W2076819067","https://openalex.org/W2087354432","https://openalex.org/W2095464171","https://openalex.org/W2101442845","https://openalex.org/W2102513649","https://openalex.org/W2121477971","https://openalex.org/W2133754427","https://openalex.org/W2141336956","https://openalex.org/W2150515284","https://openalex.org/W2150601291","https://openalex.org/W2413435377","https://openalex.org/W2493373062","https://openalex.org/W2494464391","https://openalex.org/W2533852048","https://openalex.org/W2556429104","https://openalex.org/W2734470663","https://openalex.org/W2899596245","https://openalex.org/W2900988474","https://openalex.org/W2901004948","https://openalex.org/W2990891755","https://openalex.org/W3003713259","https://openalex.org/W3011107589","https://openalex.org/W3028660636","https://openalex.org/W4242360498"],"related_works":["https://openalex.org/W2030975239","https://openalex.org/W2990891755","https://openalex.org/W3165032053","https://openalex.org/W3163686829","https://openalex.org/W1120245080","https://openalex.org/W2076819067","https://openalex.org/W135508649","https://openalex.org/W4247888148","https://openalex.org/W2130213909","https://openalex.org/W2275519973"],"abstract_inverted_index":{"Abstract":[0],"Rational":[1],"design":[2],"of":[3,16,21,28,36,39,56,63,94,109,115,119,136,139],"pharmaceutical":[4],"drugs":[5],"targeting":[6],"integral":[7],"membrane":[8],"G":[9],"protein-coupled":[10],"receptors":[11],"(GPCR)":[12],"requires":[13],"thorough":[14],"understanding":[15],"ligand":[17],"binding":[18],"and":[19,85,113],"mechanism":[20],"activation":[22],"through":[23],"high":[24,77,140],"resolution":[25],"structural":[26],"studies":[27,147],"purified":[29,64,86],"proteins.":[30],"Due":[31],"to":[32,100,105],"inherent":[33],"conformational":[34],"flexibility":[35],"GPCR,":[37],"stabilization":[38,62],"these":[40],"proteins":[41],"solubilized":[42,84],"from":[43,107],"cell":[44,82],"membranes":[45],"into":[46],"detergents":[47],"is":[48,132],"a":[49],"challenging":[50],"task.":[51],"Here,":[52],"we":[53,122],"take":[54],"advantage":[55],"naturally":[57],"occurring":[58],"post-translational":[59],"modifications":[60],"for":[61,134,146],"GPCR":[65,145],"in":[66,79,87],"detergent":[67],"micelles.":[68],"The":[69],"recombinant":[70],"cannabinoid":[71],"CB2":[72],"receptor":[73,98],"was":[74],"expressed":[75],"at":[76],"yield":[78],"Expi293F":[80,127],"mammalian":[81,96,126],"cultures,":[83],"Fa\u00e7ade":[88],"detergent.":[89],"We":[90],"report":[91],"superior":[92],"stability":[93],"the":[95,110,116,125],"cell-expressed":[97],"compared":[99],"its":[101],"E.coli-expressed":[102],"counterpart,":[103],"due":[104],"contributions":[106],"glycosylation":[108],"N":[111],"terminus":[112,118],"palmitoylation":[114],"C":[117],"CB2.":[120],"Finally,":[121],"demonstrate":[123],"that":[124],"amino":[128],"acid":[129],"labelling":[130],"kit":[131],"suitable":[133],"preparation":[135],"multi-milligram":[137],"quantities":[138],"quality,":[141],"selectively":[142],"stable":[143],"isotope-labeled":[144],"by":[148],"nuclear":[149],"magnetic":[150],"resonance.":[151]},"counts_by_year":[{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2020-10-15T00:00:00"}