{"id":"https://openalex.org/W3089851130","doi":"https://doi.org/10.1016/j.bbrep.2020.100819","title":"The use of LipidGreen2 for visualization and quantification of intracellular Poly(3-hydroxybutyrate) in Cupriavidus necator","display_name":"The use of LipidGreen2 for visualization and quantification of intracellular Poly(3-hydroxybutyrate) in Cupriavidus necator","publication_year":2020,"publication_date":"2020-09-30","ids":{"openalex":"https://openalex.org/W3089851130","doi":"https://doi.org/10.1016/j.bbrep.2020.100819","mag":"3089851130","pmid":"https://pubmed.ncbi.nlm.nih.gov/33024843","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7528054"},"language":"en","primary_location":{"id":"doi:10.1016/j.bbrep.2020.100819","doi":"https://doi.org/10.1016/j.bbrep.2020.100819","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.bbrep.2020.100819","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2405580820301291/pdf","source":{"id":"https://openalex.org/S2764640729","display_name":"Biochemistry and Biophysics Reports","issn_l":"2405-5808","issn":["2405-5808"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","ki-jl-1","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry and Biophysics Reports","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W3089851130"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.sciencedirect.com/science/article/pii/S2405580820301291/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Alexander Kettner","orcid":"https://orcid.org/0000-0001-7062-0676","observed_orcids":["https://orcid.org/0000-0001-7062-0676"]},"institutions":[{"id":"https://openalex.org/I4210089758","display_name":"Anhalt University of Applied Sciences","ror":"https://ror.org/0076zct58","country_code":"DE","type":"education","lineage":["https://openalex.org/I4210089758"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Alexander Kettner","raw_affiliation_strings":["Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Strasse 55, 06366, Koethen, Germany"],"raw_orcid":"https://orcid.org/0000-0001-7062-0676","affiliations":[{"raw_affiliation_string":"Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Strasse 55, 06366, Koethen, Germany","institution_ids":["https://openalex.org/I4210089758"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071480678","display_name":"Carola Griehl","orcid":"https://orcid.org/0000-0003-3496-0299","observed_orcids":["https://orcid.org/0000-0003-3496-0299"]},"institutions":[{"id":"https://openalex.org/I4210089758","display_name":"Anhalt University of Applied Sciences","ror":"https://ror.org/0076zct58","country_code":"DE","type":"education","lineage":["https://openalex.org/I4210089758"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Carola Griehl","raw_affiliation_strings":["Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Strasse 55, 06366, Koethen, Germany"],"raw_orcid":"https://orcid.org/0000-0003-3496-0299","affiliations":[{"raw_affiliation_string":"Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Strasse 55, 06366, Koethen, Germany","institution_ids":["https://openalex.org/I4210089758"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5071480678"],"corresponding_institution_ids":["https://openalex.org/I4210089758"],"apc_list":{"value":1700,"currency":"USD","value_usd":1700},"apc_paid":{"value":1700,"currency":"USD","value_usd":1700},"fwci":0.5434,"has_fulltext":true,"cited_by_count":16,"citation_normalized_percentile":{"value":0.48129038,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"24","issue":null,"first_page":"100819","last_page":"100819"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10661","display_name":"biodegradable polymer synthesis and properties","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10661","display_name":"biodegradable polymer synthesis and properties","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14285","display_name":"Biopolymer Synthesis and Applications","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polyhydroxybutyrate","display_name":"polyhydroxybutyrate","score":0.9980000257492065},{"id":"https://openalex.org/keywords/cupriavidus-necator","display_name":"Cupriavidus necator","score":0.9739999771118164},{"id":"https://openalex.org/keywords/fluorescent-probes","display_name":"fluorescent probes","score":0.7639999985694885},{"id":"https://openalex.org/keywords/fluorescence-spectroscopy","display_name":"fluorescence spectroscopy","score":0.675000011920929},{"id":"https://openalex.org/keywords/3d-excitation-emission-matrix","display_name":"3D excitation-emission matrix","score":0.8529999852180481},{"id":"https://openalex.org/keywords/inner-filter-effect","display_name":"inner filter effect","score":0.8069999814033508},{"id":"https://openalex.org/keywords/bioimaging","display_name":"bioimaging","score":0.7570000290870667}],"concepts":[{"id":"https://openalex.org/C2780637011","wikidata":"https://www.wikidata.org/wiki/Q148753","display_name":"Cupriavidus necator","level":4,"score":0.9761209487915039},{"id":"https://openalex.org/C2778617687","wikidata":"https://www.wikidata.org/wiki/Q2448456","display_name":"Fluorophore","level":3,"score":0.8256291151046753},{"id":"https://openalex.org/C2910521807","wikidata":"https://www.wikidata.org/wiki/Q424469","display_name":"Poly-3-hydroxybutyrate","level":2,"score":0.6196232438087463},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5996607542037964},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.4571809768676758},{"id":"https://openalex.org/C158711907","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectroscopy","level":3,"score":0.4470580816268921},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4389641582965851},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.39299774169921875},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3607122600078583},{"id":"https://openalex.org/C73447357","wikidata":"https://www.wikidata.org/wiki/Q425079","display_name":"Polyhydroxyalkanoates","level":3,"score":0.35689154267311096},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.3390272557735443},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21188068389892578},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.20309218764305115},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.11826518177986145},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.11191770434379578},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1016/j.bbrep.2020.100819","doi":"https://doi.org/10.1016/j.bbrep.2020.100819","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.bbrep.2020.100819","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2405580820301291/pdf","source":{"id":"https://openalex.org/S2764640729","display_name":"Biochemistry and Biophysics Reports","issn_l":"2405-5808","issn":["2405-5808"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","ki-jl-1","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry and Biophysics Reports","raw_type":"journal-article"},{"id":"pmid:33024843","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33024843","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry and biophysics reports","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7528054","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC7528054/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem Biophys Rep","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:38b442d6ee784e1ebaaa873743688ecb","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/38b442d6ee784e1ebaaa873743688ecb","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biochemistry and Biophysics Reports, Vol 24, Iss , Pp 100819- (2020)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.bbrep.2020.100819","doi":"https://doi.org/10.1016/j.bbrep.2020.100819","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.bbrep.2020.100819","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2405580820301291/pdf","source":{"id":"https://openalex.org/S2764640729","display_name":"Biochemistry and Biophysics Reports","issn_l":"2405-5808","issn":["2405-5808"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","ki-jl-1","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry and Biophysics Reports","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6360352552","display_name":null,"funder_award_id":"03IHS047A","funder_id":"https://openalex.org/F4320321114","funder_display_name":"Bundesministerium f\u00fcr Bildung und Forschung"}],"funders":[{"id":"https://openalex.org/F4320321114","display_name":"Bundesministerium f\u00fcr Bildung und Forschung","ror":"https://ror.org/04pz7b180"},{"id":"https://openalex.org/F4320324211","display_name":"Ministry of Economy","ror":"https://ror.org/02fn8ac40"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3089851130.pdf","grobid_xml":"https://content.openalex.org/works/W3089851130.grobid-xml"},"referenced_works_count":33,"referenced_works":["https://openalex.org/W1245438790","https://openalex.org/W1509324135","https://openalex.org/W1924452768","https://openalex.org/W1971632242","https://openalex.org/W2000634266","https://openalex.org/W2011208261","https://openalex.org/W2025489166","https://openalex.org/W2029255765","https://openalex.org/W2031382063","https://openalex.org/W2045317650","https://openalex.org/W2051486293","https://openalex.org/W2056513301","https://openalex.org/W2064028155","https://openalex.org/W2069500216","https://openalex.org/W2076056186","https://openalex.org/W2084111763","https://openalex.org/W2087440080","https://openalex.org/W2093957069","https://openalex.org/W2113833673","https://openalex.org/W2116362148","https://openalex.org/W2119181590","https://openalex.org/W2121854397","https://openalex.org/W2129762213","https://openalex.org/W2332837880","https://openalex.org/W2348603664","https://openalex.org/W2358276897","https://openalex.org/W2503857930","https://openalex.org/W2527986277","https://openalex.org/W2568046654","https://openalex.org/W2573923809","https://openalex.org/W2763004230","https://openalex.org/W2792590822","https://openalex.org/W2907516376"],"related_works":["https://openalex.org/W1924452768","https://openalex.org/W7137013194","https://openalex.org/W1980869844","https://openalex.org/W2077844033","https://openalex.org/W2081409620","https://openalex.org/W2056513301","https://openalex.org/W2084828763","https://openalex.org/W7160423307","https://openalex.org/W2139271676"],"abstract_inverted_index":{"Numerous":[0],"studies":[1],"have":[2],"been":[3],"conducted":[4],"to":[5,21,51,98,123],"develop":[6],"a":[7,87,99,124,134,145],"rapid":[8],"protocol":[9],"for":[10,77,155],"the":[11,32,44,68,109,112,121],"quantification":[12,137],"of":[13,31,111,138,157],"poly(3-hydroxybutyrate)":[14,54,139,159],"during":[15],"bacterial":[16],"fermentation":[17],"as":[18],"an":[19],"alternative":[20],"time-consuming":[22],"gravimetric":[23],"or":[24],"analytical":[25],"methods.":[26],"Fluorescence":[27],"spectroscopy":[28],"is":[29,49,127,151],"one":[30],"most":[33],"promising":[34],"approaches.":[35],"In":[36],"this":[37],"study,":[38],"it":[39],"could":[40,116],"be":[41,117],"demonstrated":[42],"that":[43],"novel":[45],"fluorescent":[46],"probe":[47],"LipidGreen2":[48,85],"able":[50],"stain":[52],"selectively":[53],"in":[55,140],"Cupriavidus":[56],"necator.":[57],"Optimal":[58],"excitation":[59,78],"and":[60,74,79,106,119,147],"emission":[61],"wavelengths":[62],"were":[63],"evaluated":[64],"using":[65],"3D-Excitation-Emission-Matrix,":[66],"displaying":[67],"best":[69],"intensities":[70],"between":[71,103],"440-460":[72],"nm":[73,76],"490\u2013520":[75],"emission,":[80],"respectively.":[81],"The":[82,130],"lipophilic":[83],"fluorophore":[84],"showed":[86],"high":[88],"long-term":[89],"stability":[90],"even":[91],"when":[92],"incubated":[93],"under":[94],"ambient":[95],"lighting.":[96],"Due":[97],"strong":[100],"linear":[101],"relationship":[102],"side":[104],"scatter":[105],"biomass":[107],"concentration,":[108],"influence":[110],"inner":[113],"filter":[114],"effects":[115],"incorporated,":[118],"adjusting":[120],"sample":[122],"specific":[125],"OD":[126],"thus":[128],"superfluous.":[129],"developed":[131],"method":[132],"allows":[133],"very":[135],"accurate":[136],"just":[141],"15":[142],"min,":[143],"following":[144],"comprehensible":[146],"simple":[148],"protocol.":[149],"It":[150],"also":[152],"excellently":[153],"suited":[154],"bioimaging":[156],"intracellular":[158],"granules.":[160]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":2}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2020-10-08T00:00:00"}