{"id":"https://openalex.org/W30877186","doi":null,"title":"Gene sequencing for identification of Paragonimus eggs from a human case.","display_name":"Gene sequencing for identification of Paragonimus eggs from a human case.","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W30877186","mag":"30877186","pmid":"https://pubmed.ncbi.nlm.nih.gov/12567661"},"language":"en","primary_location":{"id":"pmid:12567661","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12567661","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111171830","display_name":"Zheng-shan Chang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Z S Chang","raw_affiliation_strings":["Tropical Medicine Research Center and Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tropical Medicine Research Center and Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041738869","display_name":"B Wu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B Wu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054985463","display_name":"David Blair","orcid":"https://orcid.org/0000-0002-4658-3251","observed_orcids":["https://orcid.org/0000-0002-4658-3251","https://orcid.org/0000-0002-1501-2405"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Blair","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013160986","display_name":"Y.N. Zhang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y N Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"L Hu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L Hu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039600269","display_name":"S.H. Chen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S H Chen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068991195","display_name":"M.G. Chen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M G Chen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071079286","display_name":"Zhihui Feng","orcid":"https://orcid.org/0000-0003-1281-766X","observed_orcids":["https://orcid.org/0000-0003-1281-766X","https://orcid.org/0009-0004-0925-2391"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Z Feng","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5108071593","display_name":"George M. Davis","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G M Davis","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.00500644,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"18","issue":"4","first_page":"213","last_page":"5"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11154","display_name":"Parasitic infections in humans and animals","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11154","display_name":"Parasitic infections in humans and animals","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10695","display_name":"Parasites and Host Interactions","score":0.01860000006854534,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12234","display_name":"Mollusks and Parasites Studies","score":0.01119999960064888,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/paragonimiasis","display_name":"paragonimiasis","score":0.9269999861717224},{"id":"https://openalex.org/keywords/paragonimus-westermani","display_name":"Paragonimus westermani","score":0.996999979019165},{"id":"https://openalex.org/keywords/its2-gene","display_name":"ITS2 gene","score":0.7929999828338623},{"id":"https://openalex.org/keywords/molecular-identification","display_name":"molecular identification","score":0.39500001072883606},{"id":"https://openalex.org/keywords/egg-morphology","display_name":"egg morphology","score":0.6729999780654907},{"id":"https://openalex.org/keywords/sputum","display_name":"sputum","score":0.7080000042915344}],"concepts":[{"id":"https://openalex.org/C2778083953","wikidata":"https://www.wikidata.org/wiki/Q133167","display_name":"Paragonimus westermani","level":4,"score":0.977534830570221},{"id":"https://openalex.org/C2779613767","wikidata":"https://www.wikidata.org/wiki/Q2528129","display_name":"Paragonimiasis","level":3,"score":0.9457368850708008},{"id":"https://openalex.org/C155794727","wikidata":"https://www.wikidata.org/wiki/Q2306414","display_name":"Internal transcribed spacer","level":4,"score":0.756366491317749},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7099657654762268},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5991984009742737},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.5642804503440857},{"id":"https://openalex.org/C67905577","wikidata":"https://www.wikidata.org/wiki/Q215980","display_name":"Ribosomal RNA","level":3,"score":0.5117940306663513},{"id":"https://openalex.org/C2776459540","wikidata":"https://www.wikidata.org/wiki/Q2349904","display_name":"Paragonimus","level":4,"score":0.4796774387359619},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.45738500356674194},{"id":"https://openalex.org/C2776301714","wikidata":"https://www.wikidata.org/wiki/Q259346","display_name":"Sputum","level":3,"score":0.44200462102890015},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.43932589888572693},{"id":"https://openalex.org/C186851281","wikidata":"https://www.wikidata.org/wiki/Q5156512","display_name":"Complete sequence","level":4,"score":0.42349669337272644},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.41210588812828064},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.25629723072052},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.2380884885787964},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.15228727459907532},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.10086673498153687},{"id":"https://openalex.org/C165901193","wikidata":"https://www.wikidata.org/wiki/Q3129936","display_name":"Helminths","level":2,"score":0.09891381859779358},{"id":"https://openalex.org/C2781069245","wikidata":"https://www.wikidata.org/wiki/Q12204","display_name":"Tuberculosis","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010237","descriptor_name":"Paragonimiasis","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":true},{"descriptor_ui":"D010238","descriptor_name":"Paragonimus","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D010238","descriptor_name":"Paragonimus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010238","descriptor_name":"Paragonimus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D013183","descriptor_name":"Sputum","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018509","descriptor_name":"DNA, Helminth","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D021903","descriptor_name":"DNA, Ribosomal Spacer","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"pmid:12567661","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12567661","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","raw_type":"Journal Article"},{"id":"pmh:oai:researchonline.jcu.edu.au:12817","doi":null,"is_oa":false,"landing_page_url":"https://researchonline.jcu.edu.au/12817/1/12817_Chang_et_al_2000.pdf","pdf_url":"https://researchonline.jcu.edu.au/12817/1/12817_Chang_et_al_2000.pdf","source":{"id":"https://openalex.org/S4306400519","display_name":"ResearchOnline at James Cook University (James Cook University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86467917","host_organization_name":"James Cook University","host_organization_lineage":["https://openalex.org/I86467917"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"mag:30877186","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12567661","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[{"display_name":"Case Report","id":"https://openalex.org/study-designs/case-report"}],"awards":[{"id":"https://openalex.org/G3452765505","display_name":"Emerging Helminthiases in China: Genetic Diversity,","funder_award_id":"P50AI039461","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W2775160102","https://openalex.org/W2099226787","https://openalex.org/W2042668292","https://openalex.org/W2060002976","https://openalex.org/W2033748464","https://openalex.org/W1990308283","https://openalex.org/W2095980768","https://openalex.org/W2804887814","https://openalex.org/W2904191015","https://openalex.org/W2106001183","https://openalex.org/W2001619275","https://openalex.org/W2132054486","https://openalex.org/W2011606970"],"related_works":["https://openalex.org/W2392098757","https://openalex.org/W7095957656","https://openalex.org/W2048095514","https://openalex.org/W1992760342","https://openalex.org/W1963512469","https://openalex.org/W2049930092","https://openalex.org/W2049388338","https://openalex.org/W2385670776","https://openalex.org/W2105201699","https://openalex.org/W3031149541"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"identify":[2],"the":[3,68,71,79,86,89,104,107,113,116],"etiologic":[4],"agent":[5],"from":[6,30,44,53,78,88,94,106,115,122,135],"a":[7,31],"paragonimiasis":[8,32,136],"patient":[9,33,129],"using":[10],"molecular":[11],"techniques.":[12],"METHODS:":[13],"The":[14,75,128],"complete":[15],"nuclear":[16],"ribosomal":[17],"DNA":[18],"second":[19],"internal":[20],"transcribed":[21],"spacer":[22],"(ITS2)":[23],"gene":[24,77,139],"sequence":[25,87,105,140],"of":[26,46,70,91,109],"eggs":[27,45,69,90,108,114],"in":[28],"sputum":[29],"was":[34,82,130],"obtained":[35],"by":[36,138],"directly":[37],"auto-sequencing":[38],"its":[39],"PCR":[40],"product.":[41],"ITS2":[42,76],"genes":[43],"Paragonimus":[47],"westermani":[48,93,124,137],"and":[49],"Pagumogonimus":[50],"skrjabini":[51],"(both":[52],"animal":[54],"hosts)":[55],"were":[56,65],"also":[57],"sequenced":[58],"for":[59],"comparison.":[60],"In":[61],"addition,":[62],"morphological":[63],"comparisons":[64],"made":[66],"with":[67,85,103],"two":[72],"species.":[73],"RESULTS:":[74],"human":[80,117],"case":[81,118],"100%":[83],"identical":[84,102],"P.":[92,110,123],"an":[95],"experimentally":[96],"infected":[97,125],"dog":[98],"but":[99],"only":[100],"92%":[101],"skrjabini.":[111],"Morphologically,":[112],"more":[119],"resembled":[120],"those":[121],"dog.":[126],"CONCLUSION:":[127],"diagnosed":[131],"to":[132],"be":[133],"suffered":[134],"analysis.":[141]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}