{"id":"https://openalex.org/W3087569299","doi":"https://doi.org/10.3390/ijms21186865","title":"Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases","display_name":"Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases","publication_year":2020,"publication_date":"2020-09-18","ids":{"openalex":"https://openalex.org/W3087569299","doi":"https://doi.org/10.3390/ijms21186865","mag":"3087569299","pmid":"https://pubmed.ncbi.nlm.nih.gov/32962129","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7557733"},"language":"en","primary_location":{"id":"doi:10.3390/ijms21186865","doi":"https://doi.org/10.3390/ijms21186865","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms21186865","pdf_url":"https://www.mdpi.com/1422-0067/21/18/6865/pdf?version=1600941936","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1422-0067/21/18/6865/pdf?version=1600941936","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000031944","display_name":"Sergey Brezgin","orcid":"https://orcid.org/0000-0003-4792-0739","observed_orcids":["https://orcid.org/0000-0003-4792-0739"]},"institutions":[{"id":"https://openalex.org/I64264942","display_name":"Federal Medical-Biological Agency","ror":"https://ror.org/052ay8m85","country_code":"RU","type":"government","lineage":["https://openalex.org/I64264942"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Sergey Brezgin","raw_affiliation_strings":["Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia","Department of Molecular Immunology, Institute of Immunology, Federal Medical Biological Agency, 115522 Moscow, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia","institution_ids":[]},{"raw_affiliation_string":"Department of Molecular Immunology, Institute of Immunology, Federal Medical Biological Agency, 115522 Moscow, Russia","institution_ids":["https://openalex.org/I64264942"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041824825","display_name":"Anastasiya Kostyusheva","orcid":"https://orcid.org/0000-0002-2335-6582","observed_orcids":["https://orcid.org/0000-0002-2335-6582"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Anastasiya Kostyusheva","raw_affiliation_strings":["Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026864719","display_name":"N. I. Ponomareva","orcid":"https://orcid.org/0000-0002-4233-0062","observed_orcids":["https://orcid.org/0000-0002-4233-0062"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Natalia Ponomareva","raw_affiliation_strings":["Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049990908","display_name":"Viktoriia Volia","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Viktoriia Volia","raw_affiliation_strings":["Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073070496","display_name":"Irina A. Goptar","orcid":"https://orcid.org/0000-0002-7445-8044","observed_orcids":["https://orcid.org/0000-0002-7445-8044"]},"institutions":[{"id":"https://openalex.org/I4210158794","display_name":"The Federal State Budgetary Scientific Institution \"Izmerov Research Institute of Occupational Health\"","ror":"https://ror.org/04skssb35","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210110862","https://openalex.org/I4210158794"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Irina Goptar","raw_affiliation_strings":["Izmerov Research Institute of Occupational Health, 105275 Moscow, Russia"],"raw_orcid":"https://orcid.org/0000-0002-7445-8044","affiliations":[{"raw_affiliation_string":"Izmerov Research Institute of Occupational Health, 105275 Moscow, Russia","institution_ids":["https://openalex.org/I4210158794"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055323470","display_name":"Anastasiya Nikiforova","orcid":"https://orcid.org/0000-0001-6279-2101","observed_orcids":["https://orcid.org/0000-0001-6279-2101"]},"institutions":[{"id":"https://openalex.org/I4210158794","display_name":"The Federal State Budgetary Scientific Institution \"Izmerov Research Institute of Occupational Health\"","ror":"https://ror.org/04skssb35","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210110862","https://openalex.org/I4210158794"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Anastasiya Nikiforova","raw_affiliation_strings":["Izmerov Research Institute of Occupational Health, 105275 Moscow, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Izmerov Research Institute of Occupational Health, 105275 Moscow, Russia","institution_ids":["https://openalex.org/I4210158794"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027331468","display_name":"Igor P. Shilovskiy","orcid":"https://orcid.org/0000-0001-5343-4230","observed_orcids":["https://orcid.org/0000-0001-5343-4230"]},"institutions":[{"id":"https://openalex.org/I64264942","display_name":"Federal Medical-Biological Agency","ror":"https://ror.org/052ay8m85","country_code":"RU","type":"government","lineage":["https://openalex.org/I64264942"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Igor Shilovskiy","raw_affiliation_strings":["Department of Molecular Immunology, Institute of Immunology, Federal Medical Biological Agency, 115522 Moscow, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Immunology, Institute of Immunology, Federal Medical Biological Agency, 115522 Moscow, Russia","institution_ids":["https://openalex.org/I64264942"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103076195","display_name":"Smirnov Vv","orcid":"https://orcid.org/0000-0002-8232-6682","observed_orcids":["https://orcid.org/0000-0002-8232-6682"]},"institutions":[{"id":"https://openalex.org/I64264942","display_name":"Federal Medical-Biological Agency","ror":"https://ror.org/052ay8m85","country_code":"RU","type":"government","lineage":["https://openalex.org/I64264942"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Valery Smirnov","raw_affiliation_strings":["Department of Molecular Immunology, Institute of Immunology, Federal Medical Biological Agency, 115522 Moscow, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Immunology, Institute of Immunology, Federal Medical Biological Agency, 115522 Moscow, Russia","institution_ids":["https://openalex.org/I64264942"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040462277","display_name":"Dmitry S. Kostyushev","orcid":"https://orcid.org/0000-0002-1851-7441","observed_orcids":["https://orcid.org/0000-0002-1851-7441"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Dmitry Kostyushev","raw_affiliation_strings":["Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia"],"raw_orcid":"https://orcid.org/0000-0002-1851-7441","affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5001376175","display_name":"Vladimir Chulanov","orcid":"https://orcid.org/0000-0001-6303-9293","observed_orcids":["https://orcid.org/0000-0001-6303-9293"]},"institutions":[{"id":"https://openalex.org/I17877952","display_name":"Sechenov University","ror":"https://ror.org/02yqqv993","country_code":"RU","type":"education","lineage":["https://openalex.org/I17877952"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Vladimir Chulanov","raw_affiliation_strings":["Department of Infectious Diseases, Sechenov First Moscow State Medical University, 119146 Moscow, Russia","Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia"],"raw_orcid":"https://orcid.org/0000-0001-6303-9293","affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases, Sechenov First Moscow State Medical University, 119146 Moscow, Russia","institution_ids":["https://openalex.org/I17877952"]},{"raw_affiliation_string":"Department of Molecular Biology and Immunopathology of Infectious Diseases, National Medical Research Center for Tuberculosis and Infectious Diseases, 127994 Moscow, Russia","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5040462277"],"corresponding_institution_ids":[],"apc_list":{"value":2000,"currency":"CHF","value_usd":2112},"apc_paid":{"value":2000,"currency":"CHF","value_usd":2112},"fwci":0.3568,"has_fulltext":true,"cited_by_count":11,"citation_normalized_percentile":{"value":0.46045995,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":97},"biblio":{"volume":"21","issue":"18","first_page":"6865","last_page":"6865"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11668","display_name":"interferon and immune responses","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-activation","display_name":"CRISPR activation","score":0.902999997138977},{"id":"https://openalex.org/keywords/apobec3a","display_name":"APOBEC3A","score":0.9729999899864197},{"id":"https://openalex.org/keywords/apobec3b","display_name":"APOBEC3B","score":1.0},{"id":"https://openalex.org/keywords/cytidine-deaminase","display_name":"cytidine deaminase","score":0.9750000238418579},{"id":"https://openalex.org/keywords/episomal-dna","display_name":"episomal DNA","score":0.8429999947547913},{"id":"https://openalex.org/keywords/innate-immunity","display_name":"innate immunity","score":0.6330000162124634},{"id":"https://openalex.org/keywords/gc-rich-dna","display_name":"GC-rich DNA","score":0.6639999747276306},{"id":"https://openalex.org/keywords/dna-deamination","display_name":"DNA deamination","score":0.6150000095367432},{"id":"https://openalex.org/keywords/transcriptional-activation","display_name":"transcriptional activation","score":0.7020000219345093},{"id":"https://openalex.org/keywords/hek293t-cells","display_name":"HEK293T cells","score":0.8109999895095825}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7526943683624268},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6873851418495178},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.6179697513580322},{"id":"https://openalex.org/C2781184954","wikidata":"https://www.wikidata.org/wiki/Q422538","display_name":"Cytidine","level":3,"score":0.5079842209815979},{"id":"https://openalex.org/C2776985229","wikidata":"https://www.wikidata.org/wiki/Q18021211","display_name":"Cytidine deaminase","level":3,"score":0.49967074394226074},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.4650111794471741},{"id":"https://openalex.org/C2776162770","wikidata":"https://www.wikidata.org/wiki/Q4653351","display_name":"APOBEC","level":4,"score":0.4645402729511261},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.4247097671031952},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.35293692350387573},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34713542461395264},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2702142000198364},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18926575779914856},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.13866055011749268},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0635606050491333}],"mesh":[{"descriptor_ui":"D000099224","descriptor_name":"p300-CBP-Associated Factor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003564","descriptor_name":"Cytidine Deaminase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003564","descriptor_name":"Cytidine Deaminase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007113","descriptor_name":"Immunity, Innate","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015778","descriptor_name":"Minor Histocompatibility Antigens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015778","descriptor_name":"Minor Histocompatibility Antigens","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015854","descriptor_name":"Up-Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D050880","descriptor_name":"p300-CBP Transcription Factors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D050880","descriptor_name":"p300-CBP Transcription Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.3390/ijms21186865","doi":"https://doi.org/10.3390/ijms21186865","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms21186865","pdf_url":"https://www.mdpi.com/1422-0067/21/18/6865/pdf?version=1600941936","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},{"id":"pmid:32962129","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32962129","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of molecular sciences","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7557733","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC7557733/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Mol Sci","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:4698fe446b334384997aba67cbda6a0b","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/4698fe446b334384997aba67cbda6a0b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"International Journal of Molecular Sciences, Vol 21, Iss 6865, p 6865 (2020)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/ijms21186865","doi":"https://doi.org/10.3390/ijms21186865","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms21186865","pdf_url":"https://www.mdpi.com/1422-0067/21/18/6865/pdf?version=1600941936","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7430251454","display_name":null,"funder_award_id":"20-15-00373","funder_id":"https://openalex.org/F4320324099","funder_display_name":"Russian Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320324099","display_name":"Russian Science Foundation","ror":"https://ror.org/03y2gwe85"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3087569299.pdf","grobid_xml":"https://content.openalex.org/works/W3087569299.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W1521072910","https://openalex.org/W1799137450","https://openalex.org/W1821896370","https://openalex.org/W1964963969","https://openalex.org/W1966343819","https://openalex.org/W1968368569","https://openalex.org/W1969593814","https://openalex.org/W1977012657","https://openalex.org/W1988429158","https://openalex.org/W1995417160","https://openalex.org/W1997069349","https://openalex.org/W2003665515","https://openalex.org/W2004465286","https://openalex.org/W2009047406","https://openalex.org/W2010290700","https://openalex.org/W2017161741","https://openalex.org/W2036176224","https://openalex.org/W2042651904","https://openalex.org/W2045661073","https://openalex.org/W2047365750","https://openalex.org/W2051173965","https://openalex.org/W2063337242","https://openalex.org/W2066783444","https://openalex.org/W2083093678","https://openalex.org/W2087661745","https://openalex.org/W2099610218","https://openalex.org/W2101749257","https://openalex.org/W2102067890","https://openalex.org/W2115819181","https://openalex.org/W2124371255","https://openalex.org/W2127713363","https://openalex.org/W2128016314","https://openalex.org/W2174662127","https://openalex.org/W2186907268","https://openalex.org/W2202193919","https://openalex.org/W2259873134","https://openalex.org/W2353041718","https://openalex.org/W2400411104","https://openalex.org/W2589646886","https://openalex.org/W2790550266","https://openalex.org/W2888601619","https://openalex.org/W2895729614","https://openalex.org/W2912162884","https://openalex.org/W2916127337","https://openalex.org/W2918864126","https://openalex.org/W2935342951","https://openalex.org/W2946813709","https://openalex.org/W2962454748","https://openalex.org/W2982558795","https://openalex.org/W2989542822","https://openalex.org/W2989928744","https://openalex.org/W4234464746"],"related_works":["https://openalex.org/W2115819181","https://openalex.org/W2041818693","https://openalex.org/W6963864367","https://openalex.org/W3189317120","https://openalex.org/W7214435512","https://openalex.org/W4384830487","https://openalex.org/W1967215131","https://openalex.org/W1970398080","https://openalex.org/W303704380","https://openalex.org/W2771262431"],"abstract_inverted_index":{"Restriction":[0],"of":[1,17,32,46,76,113,159],"foreign":[2,97,120,165],"DNA":[3,31,124,136],"is":[4],"a":[5,77],"fundamental":[6],"defense":[7],"mechanism":[8],"required":[9],"for":[10,53,58,65,151],"maintaining":[11],"genomic":[12],"stability":[13],"and":[14,34,37,61,64,82,86,89,95,128,155],"proper":[15],"function":[16],"mammalian":[18],"cells.":[19,109],"APOBEC":[20,47],"cytidine":[21],"deaminases":[22,88],"are":[23],"crucial":[24,50],"effector":[25,162],"molecules":[26,163],"involved":[27],"in":[28,107],"clearing":[29],"pathogenic":[30],"viruses":[33],"other":[35],"microorganisms":[36],"improperly":[38],"localized":[39],"self-DNA":[40],"(DNA":[41],"leakages).":[42],"Mastering":[43],"the":[44,49,134,144,157,160],"expression":[45],"provides":[48],"means":[51],"both":[52],"developing":[54],"novel":[55],"therapeutic":[56],"approaches":[57],"combating":[59],"infectious":[60],"non-infectious":[62],"diseases":[63],"numerous":[66],"research":[67],"purposes.":[68],"In":[69],"this":[70],"study,":[71],"we":[72],"report":[73],"successful":[74],"application":[75],"CRISPRa":[78,145],"approach":[79,146],"to":[80],"effectively":[81],"specifically":[83],"overexpress":[84],"APOBEC3A":[85],"APOBEC3B":[87],"describe":[90],"their":[91],"effects":[92,158],"on":[93,164],"episomal":[94,116],"integrated":[96,119],"DNA.":[98,121],"This":[99],"method":[100],"increased":[101],"target":[102],"gene":[103],"transcription":[104],"by":[105,130],">6-50-fold":[106],"HEK293T":[108],"Furthermore,":[110],"CRISPRa-mediated":[111],"activation":[112],"APOBEC3A/APOBEC3B":[114],"suppressed":[115],"but":[117],"not":[118],"Episomal":[122],"GC-rich":[123],"was":[125],"rapidly":[126],"destabilized":[127],"destroyed":[129],"CRISPRa-induced":[131],"APOBEC3A/APOBEC3B,":[132],"while":[133],"remaining":[135],"templates":[137],"harbored":[138],"frequent":[139],"deaminated":[140],"nucleotides.":[141],"To":[142],"conclude,":[143],"could":[147],"be":[148],"readily":[149],"utilized":[150],"manipulating":[152],"innate":[153],"immunity":[154],"investigating":[156],"key":[161],"nucleic":[166],"acids.":[167]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2020-09-25T00:00:00"}