{"id":"https://openalex.org/W3087128178","doi":"https://doi.org/10.4155/bio-2020-0112","title":"A Whole Blood Microsampling Assay for Vancomycin: Development, Validation and Application for Pediatric Clinical Study","display_name":"A Whole Blood Microsampling Assay for Vancomycin: Development, Validation and Application for Pediatric Clinical Study","publication_year":2020,"publication_date":"2020-09-01","ids":{"openalex":"https://openalex.org/W3087128178","doi":"https://doi.org/10.4155/bio-2020-0112","mag":"3087128178","pmid":"https://pubmed.ncbi.nlm.nih.gov/32945688","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7607392"},"language":"en","primary_location":{"id":"doi:10.4155/bio-2020-0112","doi":"https://doi.org/10.4155/bio-2020-0112","is_oa":false,"landing_page_url":"https://doi.org/10.4155/bio-2020-0112","pdf_url":null,"source":{"id":"https://openalex.org/S159955403","display_name":"Bioanalysis","issn_l":"1757-6180","issn":["1757-6180","1757-6199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7607392","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067159899","display_name":"Ganesh S. Moorthy","orcid":"https://orcid.org/0000-0002-8779-6034","observed_orcids":["https://orcid.org/0000-0002-8779-6034"]},"institutions":[{"id":"https://openalex.org/I1335321130","display_name":"Children's Hospital of Philadelphia","ror":"https://ror.org/01z7r7q48","country_code":"US","type":"funder","lineage":["https://openalex.org/I1335321130"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ganesh S Moorthy","raw_affiliation_strings":["Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA","Department of Anesthesiology & Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA19104, USA"],"raw_orcid":"https://orcid.org/0000-0002-8779-6034","affiliations":[{"raw_affiliation_string":"Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA","institution_ids":["https://openalex.org/I1335321130"]},{"raw_affiliation_string":"Department of Anesthesiology & Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA19104, USA","institution_ids":["https://openalex.org/I1335321130"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031607533","display_name":"Kevin J. Downes","orcid":"https://orcid.org/0000-0001-5015-8146","observed_orcids":["https://orcid.org/0000-0001-5015-8146"]},"institutions":[{"id":"https://openalex.org/I1335321130","display_name":"Children's Hospital of Philadelphia","ror":"https://ror.org/01z7r7q48","country_code":"US","type":"funder","lineage":["https://openalex.org/I1335321130"]},{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin J Downes","raw_affiliation_strings":["Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA","Department of Pediatrics, University of Pennsylvania, Philadelphia, PA19104, USA"],"raw_orcid":"https://orcid.org/0000-0001-5015-8146","affiliations":[{"raw_affiliation_string":"Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA","institution_ids":["https://openalex.org/I1335321130"]},{"raw_affiliation_string":"Department of Pediatrics, University of Pennsylvania, Philadelphia, PA19104, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032161530","display_name":"Christina Vedar","orcid":"https://orcid.org/0000-0002-8310-4715","observed_orcids":["https://orcid.org/0000-0002-8310-4715"]},"institutions":[{"id":"https://openalex.org/I1335321130","display_name":"Children's Hospital of Philadelphia","ror":"https://ror.org/01z7r7q48","country_code":"US","type":"funder","lineage":["https://openalex.org/I1335321130"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christina Vedar","raw_affiliation_strings":["Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA"],"raw_orcid":"https://orcid.org/0000-0002-8310-4715","affiliations":[{"raw_affiliation_string":"Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA","institution_ids":["https://openalex.org/I1335321130"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5058640471","display_name":"Athena F. Zuppa","orcid":"https://orcid.org/0000-0002-8662-7474","observed_orcids":["https://orcid.org/0000-0002-8662-7474"]},"institutions":[{"id":"https://openalex.org/I1335321130","display_name":"Children's Hospital of Philadelphia","ror":"https://ror.org/01z7r7q48","country_code":"US","type":"funder","lineage":["https://openalex.org/I1335321130"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Athena F Zuppa","raw_affiliation_strings":["Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA","Department of Anesthesiology & Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA19104, USA"],"raw_orcid":"https://orcid.org/0000-0002-8662-7474","affiliations":[{"raw_affiliation_string":"Center for Clinical Pharmacology, Children\u2019s Hospital of Philadelphia, Philadelphia, PA19104, USA","institution_ids":["https://openalex.org/I1335321130"]},{"raw_affiliation_string":"Department of Anesthesiology & Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA19104, USA","institution_ids":["https://openalex.org/I1335321130"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.9616,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.73607256,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"12","issue":"18","first_page":"1295","last_page":"1310"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12255","display_name":"Biosimilars and Bioanalytical Methods","score":0.4438000023365021,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12255","display_name":"Biosimilars and Bioanalytical Methods","score":0.4438000023365021,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.4246000051498413,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11036","display_name":"Antibiotics Pharmacokinetics and Efficacy","score":0.042100001126527786,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vancomycin","display_name":"vancomycin","score":1.0},{"id":"https://openalex.org/keywords/therapeutic-drug-monitoring","display_name":"therapeutic drug monitoring","score":0.8989999890327454},{"id":"https://openalex.org/keywords/volumetric-absorptive-microsampling","display_name":"volumetric absorptive microsampling","score":0.9739999771118164},{"id":"https://openalex.org/keywords/lc-ms-ms-method-development","display_name":"LC-MS/MS method development","score":0.800000011920929},{"id":"https://openalex.org/keywords/assay-validation","display_name":"assay validation","score":0.8299999833106995},{"id":"https://openalex.org/keywords/drug-stability","display_name":"drug stability","score":0.640999972820282},{"id":"https://openalex.org/keywords/pediatric-pharmacokinetics","display_name":"pediatric pharmacokinetics","score":0.7210000157356262}],"concepts":[{"id":"https://openalex.org/C2778980435","wikidata":"https://www.wikidata.org/wiki/Q424027","display_name":"Vancomycin","level":4,"score":0.9159786701202393},{"id":"https://openalex.org/C2780272996","wikidata":"https://www.wikidata.org/wiki/Q1261151","display_name":"Therapeutic drug monitoring","level":3,"score":0.6777837872505188},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.6149911880493164},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.533721923828125},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.4937230050563812},{"id":"https://openalex.org/C2780035454","wikidata":"https://www.wikidata.org/wiki/Q8386","display_name":"Drug","level":2,"score":0.4545899033546448},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.3617839217185974},{"id":"https://openalex.org/C177713679","wikidata":"https://www.wikidata.org/wiki/Q679690","display_name":"Intensive care medicine","level":1,"score":0.33582186698913574},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.1869029998779297},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.12596198916435242},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1032533347606659},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.088756263256073},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.054448068141937256},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000627","qualifier_name":"therapeutic use","is_major_topic":true},{"descriptor_ui":"D001800","descriptor_name":"Blood Specimen Collection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014640","descriptor_name":"Vancomycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014640","descriptor_name":"Vancomycin","qualifier_ui":"Q000627","qualifier_name":"therapeutic use","is_major_topic":true},{"descriptor_ui":"D059788","descriptor_name":"Dried Blood Spot Testing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.4155/bio-2020-0112","doi":"https://doi.org/10.4155/bio-2020-0112","is_oa":false,"landing_page_url":"https://doi.org/10.4155/bio-2020-0112","pdf_url":null,"source":{"id":"https://openalex.org/S159955403","display_name":"Bioanalysis","issn_l":"1757-6180","issn":["1757-6180","1757-6199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7607392","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7607392","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"Text"},{"id":"pmid:32945688","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32945688","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:7607392","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7607392","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9789000153541565}],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000891309","display_name":"Institutional Clinical and Translational Science Award","funder_award_id":"UL1TR001878","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G993522125","display_name":"Optimizing Vancomycin Therapy in Children","funder_award_id":"K23HD091365","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337472","display_name":"National Center for Advancing Translational Sciences","ror":"https://ror.org/04pw6fb54"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W81552516","https://openalex.org/W1600054053","https://openalex.org/W1629505788","https://openalex.org/W1974280087","https://openalex.org/W1987149305","https://openalex.org/W1998307146","https://openalex.org/W2002616398","https://openalex.org/W2011636549","https://openalex.org/W2027798661","https://openalex.org/W2053066686","https://openalex.org/W2055576647","https://openalex.org/W2058634855","https://openalex.org/W2078235577","https://openalex.org/W2089520056","https://openalex.org/W2092950207","https://openalex.org/W2099365065","https://openalex.org/W2100191752","https://openalex.org/W2108433768","https://openalex.org/W2124130495","https://openalex.org/W2133041054","https://openalex.org/W2133120422","https://openalex.org/W2136457707","https://openalex.org/W2140796490","https://openalex.org/W2143018476","https://openalex.org/W2152575430","https://openalex.org/W2184049040","https://openalex.org/W2313609946","https://openalex.org/W2372510539","https://openalex.org/W2413535420","https://openalex.org/W2524407442","https://openalex.org/W2583680797","https://openalex.org/W2586459096","https://openalex.org/W2591665074","https://openalex.org/W2611704962","https://openalex.org/W2739481784","https://openalex.org/W2740174481","https://openalex.org/W2770880133","https://openalex.org/W2793526005","https://openalex.org/W2891000125","https://openalex.org/W2895397974","https://openalex.org/W2904701131","https://openalex.org/W2905457919","https://openalex.org/W2921395448","https://openalex.org/W2954012220","https://openalex.org/W2959671198","https://openalex.org/W2990688927","https://openalex.org/W2996076216","https://openalex.org/W3012260518"],"related_works":["https://openalex.org/W4200301338","https://openalex.org/W2904701131","https://openalex.org/W4391452367","https://openalex.org/W2947469527","https://openalex.org/W4388921837","https://openalex.org/W4317780531","https://openalex.org/W4297894079","https://openalex.org/W2739481784","https://openalex.org/W4315433341","https://openalex.org/W2413535420"],"abstract_inverted_index":{"Background:":[0],"Vancomycin":[1,54],"is":[2,83],"a":[3],"commonly":[4],"used":[5],"antibiotic,":[6],"which":[7],"requires":[8],"therapeutic":[9,26],"drug":[10,27],"monitoring":[11],"to":[12],"ensure":[13],"optimal":[14],"treatment.":[15],"Microsampling":[16],"assays":[17],"are":[18,106],"attractive":[19],"tools":[20],"for":[21,33,66,70,87,96],"pediatric":[22,99],"clinical":[23,100,104],"research":[24],"and":[25,52,102],"monitoring.":[28],"Results:":[29],"A":[30],"LC\u2013MS/MS":[31],"method":[32,82],"the":[34,88],"quantification":[35],"of":[36,90],"vancomycin":[37,67,91],"in":[38,57,92],"human":[39,58],"whole":[40,59,93],"blood":[41,60,94],"employing":[42],"volumetric":[43],"absorptive":[44],"microsampling":[45],"(VAMS\u00ae)":[46],"devices":[47],"(20":[48],"\u03bcl)":[49],"was":[50,55,68],"developed":[51],"validated.":[53],"stable":[56],"VAMS":[61,95],"under":[62],"assay":[63],"conditions.":[64],"Stability":[65],"established":[69],"at":[71,78],"least":[72],"160":[73],"days":[74],"as":[75],"dried":[76],"microsamples":[77],"-78\u00b0C.":[79],"Conclusion:":[80],"This":[81],"currently":[84],"being":[85],"utilized":[86],"quantitation":[89],"an":[97],"ongoing":[98],"study":[101],"representative":[103],"data":[105],"reported.":[107]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2020-09-25T00:00:00"}