{"id":"https://openalex.org/W3086288554","doi":null,"title":"Estrutura e propriedades de inibidores de serinoprote\u00ednases do tipo Bowman-Birk de sementes de Dioclea glabra e do tipo Kunitz de sementes de Bauhinia rufa","display_name":"Estrutura e propriedades de inibidores de serinoprote\u00ednases do tipo Bowman-Birk de sementes de Dioclea glabra e do tipo Kunitz de sementes de Bauhinia rufa","publication_year":1998,"publication_date":"1998-01-01","ids":{"openalex":"https://openalex.org/W3086288554","mag":"3086288554"},"language":"es","primary_location":{"id":"pmh:oai:repositorio.unifesp.br:11600/16063","doi":null,"is_oa":false,"landing_page_url":"http://repositorio.unifesp.br/handle/11600/16063","pdf_url":null,"source":{"id":"https://openalex.org/S4377196380","display_name":"UNIFESP Institutional Repository (Universidade Federal de S\u00e3o Paulo)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I88273585","host_organization_name":"Universidade Federal de S\u00e3o Paulo","host_organization_lineage":["https://openalex.org/I88273585"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Tese de doutorado"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083448441","display_name":"Norlene Regina Bueno","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Norlene Regina Bueno","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5083448441"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13725","display_name":"Pineapple and bromelain studies","score":0.006099999882280827,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/kunitz-trypsin-inhibitor","display_name":"Kunitz trypsin inhibitor","score":0.7770000100135803},{"id":"https://openalex.org/keywords/bauhinia-rufa","display_name":"Bauhinia rufa","score":0.9990000128746033},{"id":"https://openalex.org/keywords/seeds","display_name":"seeds","score":0.46799999475479126},{"id":"https://openalex.org/keywords/trypsin-inhibitor","display_name":"trypsin inhibitor","score":0.5989999771118164},{"id":"https://openalex.org/keywords/protease-inhibitors","display_name":"protease inhibitors","score":0.46299999952316284},{"id":"https://openalex.org/keywords/primary-structure","display_name":"primary structure","score":0.6150000095367432},{"id":"https://openalex.org/keywords/inhibitor-specificity","display_name":"inhibitor specificity","score":0.47099998593330383},{"id":"https://openalex.org/keywords/trypsin","display_name":"trypsin","score":0.367000013589859},{"id":"https://openalex.org/keywords/chymotrypsin","display_name":"chymotrypsin","score":0.75},{"id":"https://openalex.org/keywords/fabaceae","display_name":"Fabaceae","score":0.42399999499320984},{"id":"https://openalex.org/keywords/papilionoideae","display_name":"Papilionoideae","score":0.9279999732971191}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5032314658164978}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repositorio.unifesp.br:11600/16063","doi":null,"is_oa":false,"landing_page_url":"http://repositorio.unifesp.br/handle/11600/16063","pdf_url":null,"source":{"id":"https://openalex.org/S4377196380","display_name":"UNIFESP Institutional Repository (Universidade Federal de S\u00e3o Paulo)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I88273585","host_organization_name":"Universidade Federal de S\u00e3o Paulo","host_organization_lineage":["https://openalex.org/I88273585"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Tese de doutorado"},{"id":"mag:3086288554","doi":null,"is_oa":false,"landing_page_url":"http://repositorio.unifesp.br/handle/11600/16063","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2020085088","https://openalex.org/W2246277063","https://openalex.org/W1620771247","https://openalex.org/W1667646596","https://openalex.org/W2241940796","https://openalex.org/W2561393607","https://openalex.org/W7120881392","https://openalex.org/W1999889348","https://openalex.org/W1023373107","https://openalex.org/W2078125544"],"abstract_inverted_index":{"Dioclea":[0,44,251],"glabra":[1,45],"e":[2,22,54,68,89,90,95,148,157,176,183,202,212,231,262,297,317,323,330,345,351],"uma":[3,19,203],"leguminosa":[4],"da":[5,97,160,223,240],"subfamilia":[6],"Papilionoideae,":[7],"encontrada":[8],"em":[9,15,51,56,128],"todo":[10],"o":[11,74,91,103,122,191,254],"Brasil.":[12],"A":[13,58,286,334,343,357],"presenca,":[14],"suas":[16],"sementes,":[17],"de":[18,23,27,43,66,70,76,81,87,141,172,194,208,242,250,277,283,302,328,336,338,355],"proteinase":[20],"(dioclen)":[21],"um":[24],"especifico":[25],"inibidor":[26,38,75,93,104,193,255],"tripsina,":[28,138,165,303],"ja":[29],"foi":[30,39,118,150,340],"por":[31,46,48,64,110,113,228,264,347],"nos":[32,187],"previamente":[33],"descrito":[34,198],"(Bueno":[35],"1994).":[36],"O":[37],"purificado":[40],"das":[41,248,281,294],"sementes":[42,249,282],"precipitacao":[47],"acetona,":[49],"fracionamento":[50],"troca":[52],"ionica":[53],"filtracao":[55],"gel.":[57],"estrutura":[59],"primaria":[60],"do":[61,185],"inibidor,":[62],"determinada":[63],"degradacao":[65],"Edman":[67],"espectrometria":[69],"massa,":[71],"indicou":[72],"que":[73,120,213],"tripsina":[77,114],"pertence":[78],"a":[79,164,169,235,259,292],"familia":[80,241],"inibidores":[82,273,300],"Bowman-Birk,":[83],"contem":[84],"67":[85],"residuos":[86],"aminoacidos":[88,337],"primeiro":[92,123,192],"caracterizado":[94],"preparado":[96],"tribo":[98],"Dioclea:":[99],"SSGPCCDRCRCTKSEPPQCQCQDVRLNSCHSACEACVCSHSMPGLCSC":[100],"LDITHFCHEPCKSSGDDED":[101],"Embora":[102,219],"apresente":[105],"dois":[106],"sitios":[107],"reativos":[108],"determinados":[109],"clivagem":[111],"especifica":[112],"(Lys":[115],"13":[116,127],"His4o),":[117],"demonstrado":[119],"apenas":[121],"sitio,":[124],"com":[125,137,139,205,274],"Lys":[126],"Pl,":[129],"se":[130],"mostrou":[131],"efetivo.":[132],"DgTI":[133,166,186],"forma":[134],"complexo":[135],"1:1":[136],"constante":[140],"dissociacao":[142],"Ki":[143],"O,5":[144],"x":[145],"lO-9":[146],"M;":[147],"nao":[149,261,352],"observada":[151],"inibicao":[152,293],"para":[153,163,199,291,311],"quimotripsina,":[154],"calicreinas,":[155],"plasmina":[156],"trombina.":[158],"Apesar":[159],"alta":[161,360],"especificidade":[162],"tambem":[167,269,310],"inibiu":[168],"atividade":[170,308],"proteolitica":[171],"Musca":[173],"domestica":[174],"(Diptera)":[175],"Tenebrio":[177],"molitor":[178],"(Coleoptera).":[179],"As":[180],"caracteristicas":[181],"estruturais":[182],"inibitorias":[184],"permitem":[188,233],"classifica-lo":[189],"como":[190,238],"Bowman-Birk":[195],"tipo":[196],"I":[197],"dicotiledoneas.":[200],"Dioclen":[201,244],"endoproteinase":[204],"massa":[206,275],"molecular":[207,276],"aproximadamente":[209,278],"60":[210],"kDa":[211],"criva":[214],"Arg":[215],"na":[216],"posicao":[217],"Pl.":[218],"sua":[220],"acao":[221],"dependa":[222],"presenca":[224],"metais,":[225],"as":[226],"inibicoes":[227],"TPCK,":[229],"TLCK":[230],"DFP":[232],"caracterizar":[234],"enzima":[236,260],"tentativamente":[237],"membro":[239],"serinoproteinases.":[243,295],"hidrolisa":[245],"proteinas":[246,287],"nativas":[247],"glabra,":[252],"inclusive":[253],"DgTI;":[256],"n":[257],"entanto,":[258],"afetada":[263],"esse":[265],"inibidor.":[266],"Neste":[267],"trabalho":[268],"foram":[270,299],"caracterizados":[271],"2":[272],"20":[279],"kDa,":[280],"Bauhinia":[284],"rufa.":[285],"apresentaram":[288],"diferentes":[289],"especificidades":[290],"BrTI-l":[296,339],"BrTI-3":[298,306],"efetivos":[301],"mas":[304],"somente":[305],"apresentou":[307],"inibitoria":[309],"fator":[312],"Xa":[313],"(Ki":[314,320],"3,5":[315],"nM)":[316],"calicreinas":[318],"teciduais":[319],"1,5":[321],"nM":[322],"19":[324],"nM),":[325],"calicreina":[326],"pancreatica":[327],"porco":[329],"urinaria":[331],"humana,":[332],"respectivamente.":[333],"sequencia":[335,358],"parcialmente":[341],"determinada.":[342],"proteina":[344],"formada":[346],"cadeia":[348],"polipeptidica":[349],"unica":[350],"apresenta":[353,359],"residuo":[354],"cisteina.":[356],"...(au)":[361]},"counts_by_year":[],"updated_date":"2026-09-09T07:26:56.047234","created_date":"2020-09-21T00:00:00"}