{"id":"https://openalex.org/W3083319554","doi":"https://doi.org/10.1158/1538-7445.am2020-1596","title":"Abstract 1596: Profiling immune cell populations in the tumor microenvironment with complementary capillary-based and single-cell western assays","display_name":"Abstract 1596: Profiling immune cell populations in the tumor microenvironment with complementary capillary-based and single-cell western assays","publication_year":2020,"publication_date":"2020-08-15","ids":{"openalex":"https://openalex.org/W3083319554","doi":"https://doi.org/10.1158/1538-7445.am2020-1596","mag":"3083319554"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2020-1596","doi":"https://doi.org/10.1158/1538-7445.am2020-1596","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2020-1596","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058270282","display_name":"Charles H. Haitjema","orcid":"https://orcid.org/0000-0002-2605-6219","observed_orcids":["https://orcid.org/0000-0002-2605-6219"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Charles Haitjema","raw_affiliation_strings":["1Bio-Techne, San Jose, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Bio-Techne, San Jose, CA;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081466751","display_name":"Rainer Grant","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210149757","display_name":"R&D Systems (United States)","ror":"https://ror.org/05k887p27","country_code":"US","type":"company","lineage":["https://openalex.org/I4210149757"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rainer Grant","raw_affiliation_strings":["2Bio-Techne, Minneapolis, MN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Bio-Techne, Minneapolis, MN","institution_ids":["https://openalex.org/I4210149757"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033526030","display_name":"Lindsay Wentz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210149757","display_name":"R&D Systems (United States)","ror":"https://ror.org/05k887p27","country_code":"US","type":"company","lineage":["https://openalex.org/I4210149757"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lindsay Wentz","raw_affiliation_strings":["2Bio-Techne, Minneapolis, MN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Bio-Techne, Minneapolis, MN","institution_ids":["https://openalex.org/I4210149757"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050443171","display_name":"Stefan Luhowskyj","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210149757","display_name":"R&D Systems (United States)","ror":"https://ror.org/05k887p27","country_code":"US","type":"company","lineage":["https://openalex.org/I4210149757"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stefan Luhowskyj","raw_affiliation_strings":["2Bio-Techne, Minneapolis, MN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Bio-Techne, Minneapolis, MN","institution_ids":["https://openalex.org/I4210149757"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030500421","display_name":"Jeff Cooper","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210149757","display_name":"R&D Systems (United States)","ror":"https://ror.org/05k887p27","country_code":"US","type":"company","lineage":["https://openalex.org/I4210149757"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeff Cooper","raw_affiliation_strings":["2Bio-Techne, Minneapolis, MN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Bio-Techne, Minneapolis, MN","institution_ids":["https://openalex.org/I4210149757"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033843823","display_name":"Annegret Boge","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Annegret Boge","raw_affiliation_strings":["1Bio-Techne, San Jose, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Bio-Techne, San Jose, CA;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025680168","display_name":"Kathy Brumbaugh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210149757","display_name":"R&D Systems (United States)","ror":"https://ror.org/05k887p27","country_code":"US","type":"company","lineage":["https://openalex.org/I4210149757"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kathy Brumbaugh","raw_affiliation_strings":["2Bio-Techne, Minneapolis, MN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Bio-Techne, Minneapolis, MN","institution_ids":["https://openalex.org/I4210149757"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021098390","display_name":"Kelly Gardner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kelly Gardner","raw_affiliation_strings":["1Bio-Techne, San Jose, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Bio-Techne, San Jose, CA;","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5050091729","display_name":"Christopher D. Heger","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Christopher Heger","raw_affiliation_strings":["1Bio-Techne, San Jose, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Bio-Techne, San Jose, CA;","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"80","issue":"16_Supplement","first_page":"1596","last_page":"1596"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.1979999989271164,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.1979999989271164,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.11249999701976776,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.08479999750852585,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.9900000095367432},{"id":"https://openalex.org/keywords/immune-profiling","display_name":"immune profiling","score":0.9369999766349792},{"id":"https://openalex.org/keywords/single-cell-western-blot","display_name":"single-cell western blot","score":0.9459999799728394},{"id":"https://openalex.org/keywords/dendritic-cells","display_name":"dendritic cells","score":0.8679999709129333},{"id":"https://openalex.org/keywords/regulatory-t-cells","display_name":"regulatory T cells","score":0.9890000224113464},{"id":"https://openalex.org/keywords/natural-killer-cells","display_name":"natural killer cells","score":0.972000002861023},{"id":"https://openalex.org/keywords/dc-sign","display_name":"DC-SIGN","score":0.7590000033378601},{"id":"https://openalex.org/keywords/cd83","display_name":"CD83","score":0.9929999709129333},{"id":"https://openalex.org/keywords/cd56","display_name":"CD56","score":0.8700000047683716},{"id":"https://openalex.org/keywords/cd25","display_name":"CD25","score":0.7509999871253967},{"id":"https://openalex.org/keywords/peripheral-blood-mononuclear-cells","display_name":"peripheral blood mononuclear cells","score":0.8659999966621399},{"id":"https://openalex.org/keywords/cell-heterogeneity","display_name":"cellular heterogeneity","score":0.6679999828338623},{"id":"https://openalex.org/keywords/protein-biomarkers","display_name":"protein biomarkers","score":0.9089999794960022},{"id":"https://openalex.org/keywords/small-sample-size","display_name":"small sample size","score":0.5669999718666077},{"id":"https://openalex.org/keywords/multiplexing","display_name":"multiplexing","score":0.5929999947547913}],"concepts":[{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.6214323043823242},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5690635442733765},{"id":"https://openalex.org/C189014844","wikidata":"https://www.wikidata.org/wiki/Q189118","display_name":"Cell type","level":3,"score":0.533130407333374},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5094509720802307},{"id":"https://openalex.org/C79484868","wikidata":"https://www.wikidata.org/wiki/Q5971673","display_name":"IL-2 receptor","level":4,"score":0.5017330646514893},{"id":"https://openalex.org/C2779727006","wikidata":"https://www.wikidata.org/wiki/Q426188","display_name":"FOXP3","level":3,"score":0.4907430410385132},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4820857644081116},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.47802647948265076},{"id":"https://openalex.org/C2776107976","wikidata":"https://www.wikidata.org/wiki/Q1786433","display_name":"Tumor microenvironment","level":3,"score":0.458195298910141},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4333500266075134},{"id":"https://openalex.org/C137061746","wikidata":"https://www.wikidata.org/wiki/Q736033","display_name":"Peripheral blood mononuclear cell","level":3,"score":0.4297184646129608},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.3448001444339752},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.191510409116745},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.12137579917907715},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2020-1596","doi":"https://doi.org/10.1158/1538-7445.am2020-1596","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2020-1596","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9476000070571899}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2028464090","https://openalex.org/W2954996966","https://openalex.org/W4385687033","https://openalex.org/W4280511943","https://openalex.org/W3119943284","https://openalex.org/W2994296444","https://openalex.org/W3181518059","https://openalex.org/W4319433266","https://openalex.org/W3106715564","https://openalex.org/W3109077283"],"abstract_inverted_index":{"Abstract":[0],"The":[1,22],"tumor":[2,338],"microenvironment":[3,339],"(TME)":[4],"is":[5,49,95],"a":[6,25,43,50,223,292],"complex":[7],"mixture":[8],"of":[9,60,78,89,191,206,237,269,286,298,350,354],"cancerous":[10],"and":[11,20,30,32,56,83,157,162,181,189,198,218,229,231,251,256,283,295,309,343,365],"non-cancerous":[12],"cells,":[13,228],"including":[14],"immune":[15,127,333],"cells":[16,128,149,155,160,166,239],"like":[17,306],"T-cells,":[18],"macrophages,":[19],"neutrophils.":[21],"TME":[23,70,300],"plays":[24],"key":[26],"role":[27],"in":[28,63,131,142,254,263,336],"tumorigenesis":[29],"metastasis,":[31],"it":[33,39],"has":[34],"recently":[35],"been":[36],"recognized":[37],"that":[38,103,275],"can":[40,104],"dramatically":[41],"shape":[42],"response":[44],"to":[45,53,86,125,139],"therapy.":[46],"Thus,":[47],"there":[48],"pressing":[51],"need":[52],"accurately":[54],"identify":[55,126],"quantify":[57],"the":[58,69,76,79,98,132,187,196,203,267,276,299,337,351,355],"variety":[59],"cell":[61,91,192,271,334],"types":[62],"any":[64],"given":[65],"TME.":[66,133],"However,":[67],"studying":[68],"presents":[71],"major":[72],"challenges.":[73],"For":[74],"example,":[75,247],"heterogeneity":[77],"environment":[80],"requires":[81],"sensitive":[82],"high-resolution":[84],"techniques":[85],"parse":[87],"subpopulations":[88,253],"different":[90],"types.":[92],"This":[93],"challenge":[94],"compounded":[96],"by":[97,178,216],"severely":[99],"limited":[100],"sample":[101,121,278],"size":[102],"be":[105],"obtained":[106],"from":[107,169],"donor":[108],"tissues.":[109],"To":[110],"address":[111],"these":[112,244,287],"challenges,":[113],"we":[114,248],"use":[115],"an":[116,257],"in-capillary":[117,179,210],"immunoassay":[118,180,211],"with":[119,303,340],"small":[120,277],"sizes":[122],"(3":[123],"\u00b5L)":[124],"commonly":[129],"found":[130],"We":[134,273],"also":[135],"leverage":[136],"single-cell":[137,182,197,281,344],"western":[138,307,345],"uncover":[140],"trends":[141],"population":[143,199],"heterogeneity.":[144],"Human":[145],"peripheral":[146],"blood":[147],"mononuclear":[148],"(PBMCs)":[150],"were":[151,167,175],"differentiated":[152],"into":[153],"dendritic":[154],"(DCs)":[156],"regulatory":[158],"T":[159],"(Tregs),":[161],"natural":[163],"killer":[164],"(NK)":[165],"expanded":[168],"isolated":[170],"NK":[171,227],"cells.":[172],"These":[173,184],"samples":[174],"then":[176],"analyzed":[177],"western.":[183],"analyses":[185],"revealed":[186],"identification":[188],"characterization":[190,297],"types,":[193],"at":[194],"both":[195],"level,":[200],"based":[201],"on":[202],"differential":[204],"expression":[205,220,233],"protein":[207],"biomarkers.":[208],"Specifically,":[209],"analysis":[212],"identified":[213],"mature":[214],"populations":[215,335],"CD209":[217],"CD83":[219],"for":[221,226,234,246,358],"DCs,":[222],"CD56+/CD3-":[224],"phenotype":[225],"CD25":[230],"Foxp3":[232],"Tregs.":[235],"Analysis":[236],"single":[238],"provided":[240],"further":[241],"detail":[242],"within":[243],"populations,":[245],"observed":[249],"FoxP3low":[250],"FoxP3high":[252],"Tregs,":[255],"unexpectedly":[258],"large":[259],"(81%)":[260],"CD56-/CD3-":[261],"subpopulation":[262],"undifferentiated":[264],"PBMCs,":[265],"suggesting":[266],"presence":[268],"other":[270],"subtypes.":[272],"anticipate":[274],"size,":[279],"automation,":[280],"resolution,":[282],"multiplexing":[284],"ability":[285],"assays":[288,346],"collectively":[289],"will":[290],"enable":[291],"more":[293],"efficient":[294],"deeper":[296],"not":[301],"possible":[302],"traditional":[304],"immunoassays":[305],"blot":[308],"flow":[310],"cytometry.":[311],"Citation":[312],"Format:":[313],"Charles":[314],"Haitjema,":[315],"Rainer":[316],"Grant,":[317],"Lindsay":[318],"Wentz,":[319],"Stefan":[320],"Luhowskyj,":[321],"Jeff":[322],"Cooper,":[323],"Annegret":[324],"Boge,":[325],"Kathy":[326],"Brumbaugh,":[327],"Kelly":[328],"Gardner,":[329],"Christopher":[330],"Heger.":[331],"Profiling":[332],"complementary":[341],"capillary-based":[342],"[abstract].":[347],"In:":[348],"Proceedings":[349],"Annual":[352],"Meeting":[353],"American":[356],"Association":[357],"Cancer":[359,371],"Research":[360],"2020;":[361],"2020":[362],"Apr":[363],"27-28":[364],"Jun":[366],"22-24.":[367],"Philadelphia":[368],"(PA):":[369],"AACR;":[370],"Res":[372],"2020;80(16":[373],"Suppl):Abstract":[374],"nr":[375],"1596.":[376]},"counts_by_year":[],"updated_date":"2026-07-14T23:27:15.235271","created_date":"2020-09-11T00:00:00"}