{"id":"https://openalex.org/W3081501548","doi":"https://doi.org/10.1007/s00253-020-10826-2","title":"The hydrophobicity of an amino acid residue in a flexible loop of KP-43 protease alters activity toward a macromolecule substrate","display_name":"The hydrophobicity of an amino acid residue in a flexible loop of KP-43 protease alters activity toward a macromolecule substrate","publication_year":2020,"publication_date":"2020-08-25","ids":{"openalex":"https://openalex.org/W3081501548","doi":"https://doi.org/10.1007/s00253-020-10826-2","mag":"3081501548","pmid":"https://pubmed.ncbi.nlm.nih.gov/32840642","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7471176"},"language":"en","primary_location":{"id":"doi:10.1007/s00253-020-10826-2","doi":"https://doi.org/10.1007/s00253-020-10826-2","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-020-10826-2","pdf_url":"https://link.springer.com/content/pdf/10.1007/s00253-020-10826-2.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://link.springer.com/content/pdf/10.1007/s00253-020-10826-2.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025831049","display_name":"Mitsuyoshi Okuda","orcid":"https://orcid.org/0000-0002-9631-7808","observed_orcids":["https://orcid.org/0000-0002-9631-7808"]},"institutions":[{"id":"https://openalex.org/I207623266","display_name":"Kao Corporation (Japan)","ror":"https://ror.org/016t1kc57","country_code":"JP","type":"company","lineage":["https://openalex.org/I207623266"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Mitsuyoshi Okuda","raw_affiliation_strings":["Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan. okuda.mitsuyoshi@kao.com","Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan"],"raw_orcid":"https://orcid.org/0000-0002-9631-7808","affiliations":[{"raw_affiliation_string":"Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan. okuda.mitsuyoshi@kao.com","institution_ids":["https://openalex.org/I207623266"]},{"raw_affiliation_string":"Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan","institution_ids":["https://openalex.org/I207623266"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111740219","display_name":"Tadahiro Ozawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I207623266","display_name":"Kao Corporation (Japan)","ror":"https://ror.org/016t1kc57","country_code":"JP","type":"company","lineage":["https://openalex.org/I207623266"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tadahiro Ozawa","raw_affiliation_strings":["Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi, 321-3497, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi, 321-3497, Japan","institution_ids":["https://openalex.org/I207623266"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087734812","display_name":"Akihito Kawahara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I207623266","display_name":"Kao Corporation (Japan)","ror":"https://ror.org/016t1kc57","country_code":"JP","type":"company","lineage":["https://openalex.org/I207623266"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Akihito Kawahara","raw_affiliation_strings":["Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan","institution_ids":["https://openalex.org/I207623266"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5066250745","display_name":"Yasushi Takimura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I207623266","display_name":"Kao Corporation (Japan)","ror":"https://ror.org/016t1kc57","country_code":"JP","type":"company","lineage":["https://openalex.org/I207623266"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yasushi Takimura","raw_affiliation_strings":["Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama, 640-8580, Japan","institution_ids":["https://openalex.org/I207623266"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5025831049"],"corresponding_institution_ids":["https://openalex.org/I207623266"],"apc_list":{"value":3860,"currency":"USD","value_usd":3860},"apc_paid":{"value":3860,"currency":"USD","value_usd":3860},"fwci":0.6206,"has_fulltext":true,"cited_by_count":9,"citation_normalized_percentile":{"value":0.54730847,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"104","issue":"19","first_page":"8339","last_page":"8349"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.6963000297546387,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.6963000297546387,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.1468999981880188,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.08900000154972076,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydrophobicity","display_name":"hydrophobicity","score":0.652999997138977},{"id":"https://openalex.org/keywords/flexible-loop","display_name":"flexible loop","score":0.6449999809265137},{"id":"https://openalex.org/keywords/caseinolytic-activity","display_name":"caseinolytic activity","score":0.871999979019165},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.44600000977516174},{"id":"https://openalex.org/keywords/protein-surface-residues","display_name":"protein surface residues","score":0.6129999756813049},{"id":"https://openalex.org/keywords/substrate-binding","display_name":"substrate binding","score":0.5270000100135803},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.6110000014305115},{"id":"https://openalex.org/keywords/proteolytic-activity","display_name":"proteolytic activity","score":0.6110000014305115}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8094795942306519},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.7724192142486572},{"id":"https://openalex.org/C2776142715","wikidata":"https://www.wikidata.org/wiki/Q193970","display_name":"Casein","level":2,"score":0.6823013424873352},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.5887046456336975},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.5821996927261353},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5262369513511658},{"id":"https://openalex.org/C204389451","wikidata":"https://www.wikidata.org/wiki/Q178593","display_name":"Macromolecule","level":2,"score":0.4858369529247284},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.43816107511520386},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.429945170879364},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.4198291301727295},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4192942678928375},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.39854907989501953},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3625997006893158},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.06760314106941223},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057927","descriptor_name":"Hydrophobic and Hydrophilic Interactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1007/s00253-020-10826-2","doi":"https://doi.org/10.1007/s00253-020-10826-2","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-020-10826-2","pdf_url":"https://link.springer.com/content/pdf/10.1007/s00253-020-10826-2.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},{"id":"pmid:32840642","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32840642","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied microbiology and biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7471176","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC7471176/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Microbiol Biotechnol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1007/s00253-020-10826-2","doi":"https://doi.org/10.1007/s00253-020-10826-2","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-020-10826-2","pdf_url":"https://link.springer.com/content/pdf/10.1007/s00253-020-10826-2.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3081501548.pdf","grobid_xml":"https://content.openalex.org/works/W3081501548.grobid-xml"},"referenced_works_count":33,"referenced_works":["https://openalex.org/W153791503","https://openalex.org/W1788067121","https://openalex.org/W1970291737","https://openalex.org/W1971305937","https://openalex.org/W1979212500","https://openalex.org/W1991298672","https://openalex.org/W1992241700","https://openalex.org/W1993689973","https://openalex.org/W1998265315","https://openalex.org/W2006670577","https://openalex.org/W2007833676","https://openalex.org/W2009680000","https://openalex.org/W2010994037","https://openalex.org/W2012808851","https://openalex.org/W2024064004","https://openalex.org/W2043855562","https://openalex.org/W2048846412","https://openalex.org/W2060913542","https://openalex.org/W2065384285","https://openalex.org/W2073059515","https://openalex.org/W2075181565","https://openalex.org/W2096565822","https://openalex.org/W2100837269","https://openalex.org/W2101501929","https://openalex.org/W2109836941","https://openalex.org/W2145368473","https://openalex.org/W2324713756","https://openalex.org/W2332950537","https://openalex.org/W2791532673","https://openalex.org/W2806394880","https://openalex.org/W2887416956","https://openalex.org/W2936990387","https://openalex.org/W4210680970"],"related_works":["https://openalex.org/W2075181565","https://openalex.org/W2284461159","https://openalex.org/W2904788616","https://openalex.org/W2141352711","https://openalex.org/W1491370718","https://openalex.org/W3170384387","https://openalex.org/W2148740756","https://openalex.org/W2313466762","https://openalex.org/W1971058118","https://openalex.org/W2006714719"],"abstract_inverted_index":{"ion,":[0],"dramatically":[1],"affects":[2],"the":[3,12,45,48,58,61,73,76,90,97,105,122,132,140,143,149,170,180,185,190,202,213,234],"proteolytic":[4],"activity":[5,31,56,83,147,219],"and":[6,24,37,81,135,217],"thermal":[7],"stability":[8],"of":[9,75,107,109,162,187,212],"KP-43.":[10],"In":[11],"present":[13],"study,":[14],"we":[15,173],"obtained":[16],"20":[17,150],"variants":[18,151],"with":[19,47,60,159,189],"substitutions":[20],"at":[21,78,84,94,115,126,164],"position":[22,79,127],"195":[23,80,128],"investigated":[25],"how":[26],"these":[27],"residues":[28,178],"affect":[29],"hydrolytic":[30],"toward":[32],"a":[33,38,221],"macromolecular":[34,191],"substrate":[35,230],"(casein)":[36],"synthetic":[39],"tetra-peptide":[40],"(AAPL).":[41],"At":[42,87],"pH":[43,85,88,95,116,119,166],"10,":[44],"variant":[46,59],"highest":[49],"caseinolytic":[50,63,82,146],"activity,":[51,64,172],"Tyr195Gln,":[52],"exhibited":[53],"4.4-fold":[54],"higher":[55],"than":[57],"lowest":[62],"Tyr195Trp.":[65],"A":[66,196],"significant":[67],"negative":[68],"correlation":[69,91,98,112],"was":[70,113,152],"observed":[71,114],"between":[72,148],"hydrophobicity":[74,211],"residue":[77,125,200,216,228],"8-10.":[86],"7,":[89],"became":[92],"weak;":[93],"6,":[96],"reversed":[99],"to":[100,154,183],"positive.":[101],"Unlike":[102],"casein,":[103],"in":[104,145,156],"case":[106],"hydrolysis":[108],"AAPL,":[110],"no":[111],"10":[117],"or":[118],"6.":[120],"Because":[121],"amino":[123,176,198,214,226],"acid":[124,177,199,215,227],"is":[129],"located":[130],"on":[131,179,201,233],"protein":[133,181,203,235],"surface":[134,182,204],"considered":[136],"sufficiently":[137],"far":[138],"from":[139],"active":[141],"cleft,":[142],"variation":[144],"attributed":[153],"changes":[155],"interaction":[157,188],"efficiency":[158,186],"different":[160,165],"states":[161],"casein":[163],"values.":[167],"To":[168],"improve":[169],"enzymatic":[171],"propose":[174],"substituting":[175],"change":[184],"substrates.":[192],"KEY":[193],"POINTS:":[194],"\u2022":[195,209,223],"single":[197],"markedly":[205],"changed":[206],"enzyme":[207,218],"activity.":[208],"The":[210,224],"had":[220],"correlation.":[222],"key":[225],"for":[229],"recognition":[231],"exists":[232],"surface.":[236]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2020-09-01T00:00:00"}
