{"id":"https://openalex.org/W3059624977","doi":null,"title":"Molecular detection of Pseudomonas stutzeri by duplex-PCR technique","display_name":"Molecular detection of Pseudomonas stutzeri by duplex-PCR technique","publication_year":2019,"publication_date":"2019-01-10","ids":{"openalex":"https://openalex.org/W3059624977","mag":"3059624977"},"language":"en","primary_location":{"id":"pmh:oai:doaj.org/article:62a542b7e36b4729809fd69bcd16de69","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/62a542b7e36b4729809fd69bcd16de69","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"\u0645\u062c\u0644\u0647 \u062f\u0627\u0646\u0634\u06af\u0627\u0647 \u0639\u0644\u0648\u0645 \u067e\u0632\u0634\u06a9\u06cc \u06af\u0631\u06af\u0627\u0646, Vol 20, Iss 4, Pp 115-122 (2019)","raw_type":"article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://goums.ac.ir/journal/article-1-3543-en.html","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061772378","display_name":"Roya Beytsayyah","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I112450260","display_name":"Zabol University","ror":"https://ror.org/03d9mz263","country_code":"IR","type":"education","lineage":["https://openalex.org/I112450260"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Roya Beytsayyah","raw_affiliation_strings":["M.Sc in Genetics, Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"M.Sc in Genetics, Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran","institution_ids":["https://openalex.org/I112450260"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006140117","display_name":"Fatemeh Haddadi","orcid":"https://orcid.org/0009-0007-0712-8479","observed_orcids":["https://orcid.org/0009-0007-0712-8479","https://orcid.org/0000-0002-1957-5213"]},"institutions":[{"id":"https://openalex.org/I112450260","display_name":"Zabol University","ror":"https://ror.org/03d9mz263","country_code":"IR","type":"education","lineage":["https://openalex.org/I112450260"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Fatemeh Haddadi","raw_affiliation_strings":["Assistant Professor, Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran"],"raw_orcid":"https://orcid.org/0009-0007-0712-8479","affiliations":[{"raw_affiliation_string":"Assistant Professor, Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran","institution_ids":["https://openalex.org/I112450260"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056997045","display_name":"Hossein Kamaladini","orcid":"https://orcid.org/0000-0002-5550-9010","observed_orcids":["https://orcid.org/0000-0002-5550-9010"]},"institutions":[{"id":"https://openalex.org/I112450260","display_name":"Zabol University","ror":"https://ror.org/03d9mz263","country_code":"IR","type":"education","lineage":["https://openalex.org/I112450260"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Hossein Kamaladini","raw_affiliation_strings":["Assistant Professor, Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Assistant Professor, Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran","institution_ids":["https://openalex.org/I112450260"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112058441","display_name":"Mirza Mohammad Reza Sharifmoghadam","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86958956","display_name":"Ferdowsi University of Mashhad","ror":"https://ror.org/00g6ka752","country_code":"IR","type":"education","lineage":["https://openalex.org/I86958956"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Mirza Mohammad Reza Sharifmoghadam","raw_affiliation_strings":["Assistant Professor, Department of Biology, Faculty of Sciences, Ferdowsi University, Mashhad, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Assistant Professor, Department of Biology, Faculty of Sciences, Ferdowsi University, Mashhad, Iran","institution_ids":["https://openalex.org/I86958956"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"20","issue":"4","first_page":"115","last_page":"122"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12824","display_name":"Infections and bacterial resistance","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pseudomonas-stutzeri","display_name":"Pseudomonas stutzeri","score":1.0},{"id":"https://openalex.org/keywords/duplex-pcr","display_name":"duplex PCR","score":0.9570000171661377},{"id":"https://openalex.org/keywords/bacterial-identification","display_name":"bacterial identification","score":0.7799999713897705},{"id":"https://openalex.org/keywords/molecular-detection","display_name":"molecular detection","score":0.6119999885559082},{"id":"https://openalex.org/keywords/pcr-sensitivity-and-specificity","display_name":"PCR sensitivity and specificity","score":0.7210000157356262},{"id":"https://openalex.org/keywords/pseudomonas","display_name":"Pseudomonas","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C2776786877","wikidata":"https://www.wikidata.org/wiki/Q2888828","display_name":"Pseudomonas stutzeri","level":3,"score":0.9742517471313477},{"id":"https://openalex.org/C99611785","wikidata":"https://www.wikidata.org/wiki/Q6453233","display_name":"Duplex (building)","level":3,"score":0.5766832828521729},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.40969446301460266},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37238574028015137},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3297291100025177},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.32633328437805176},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.17846190929412842},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.09847354888916016},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.0880579948425293}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:doaj.org/article:62a542b7e36b4729809fd69bcd16de69","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/62a542b7e36b4729809fd69bcd16de69","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"\u0645\u062c\u0644\u0647 \u062f\u0627\u0646\u0634\u06af\u0627\u0647 \u0639\u0644\u0648\u0645 \u067e\u0632\u0634\u06a9\u06cc \u06af\u0631\u06af\u0627\u0646, Vol 20, Iss 4, Pp 115-122 (2019)","raw_type":"article"},{"id":"mag:3059624977","doi":null,"is_oa":true,"landing_page_url":"https://goums.ac.ir/journal/article-1-3543-en.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306537847","display_name":"\u0645\u062c\u0644\u0647 \u0639\u0644\u0645\u06cc \u062f\u0627\u0646\u0634\u06af\u0627\u0647 \u0639\u0644\u0648\u0645 \u067e\u0632\u0634\u06a9\u06cc \u06af\u0631\u06af\u0627\u0646","issn_l":"1562-4765","issn":["1562-4765","2008-4080"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Majallah-i da\u0304nishga\u0304h-i \u0312ulu\u0304m-i pizishki\u0304-i Gurga\u0304n./Majallah-i d\u0101nishg\u0101h-i \u0312ul\u016bm-i pizishk\u012b-i Gurg\u0101n.","raw_type":null}],"best_oa_location":{"id":"mag:3059624977","doi":null,"is_oa":true,"landing_page_url":"https://goums.ac.ir/journal/article-1-3543-en.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306537847","display_name":"\u0645\u062c\u0644\u0647 \u0639\u0644\u0645\u06cc \u062f\u0627\u0646\u0634\u06af\u0627\u0647 \u0639\u0644\u0648\u0645 \u067e\u0632\u0634\u06a9\u06cc \u06af\u0631\u06af\u0627\u0646","issn_l":"1562-4765","issn":["1562-4765","2008-4080"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Majallah-i da\u0304nishga\u0304h-i \u0312ulu\u0304m-i pizishki\u0304-i Gurga\u0304n./Majallah-i d\u0101nishg\u0101h-i \u0312ul\u016bm-i pizishk\u012b-i Gurg\u0101n.","raw_type":null},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2389058874","https://openalex.org/W2102874380","https://openalex.org/W2365260205","https://openalex.org/W2089756145","https://openalex.org/W3160908277","https://openalex.org/W2348800003","https://openalex.org/W2354457792","https://openalex.org/W2079506307","https://openalex.org/W2917350416","https://openalex.org/W2003576961"],"abstract_inverted_index":{"Background":[0],"and":[1,17,54,88,93,113,118,140,151,154],"Objective:":[2],"Duplex":[3,70],"PCR":[4,71,97],"is":[5],"a":[6,166],"widespread":[7],"molecular":[8,30,146],"biology":[9],"technique":[10,98],"that":[11],"has":[12],"the":[13,29,57,63,79],"ability":[14],"in":[15,62,169],"specific":[16,76],"high":[18,155],"sensitivity":[19,129,150],"detection":[20],"of":[21,32,78,95,107,110,130,133,159],"microorganisms.":[22],"This":[23],"study":[24],"was":[25,48,60,72,126,136],"performed":[26],"to":[27,175],"evaluate":[28],"identification":[31,77,158],"Pseudomonas":[33,43,160],"stutzeri":[34,44],"using":[35,67,86,101],"duplex":[36,96],"PCR.":[37],"Methods:":[38],"In":[39],"this":[40],"descriptive-laboratory":[41],"study,":[42],"ATCC":[45],"17588":[46],"bacteria":[47,80,161],"purchased":[49],"from":[50],"genetic":[51],"resources":[52],"center":[53],"after":[55],"culturing":[56],"bacteria,":[58],"DNA":[59,135],"extracted":[61],"exponential":[64],"growth":[65],"phase":[66],"boiling":[68],"method.":[69],"carried":[73],"out":[74],"for":[75,116,138,157],"subsequently.":[81],"The":[82,105,124],"primers":[83],"were":[84,99,121],"designed":[85],"catA":[87,117,139],"nirP":[89,119,141],"gene":[90],"sequences.":[91],"Sensitivity":[92],"specificity":[94,125],"investigated":[100],"5":[102],"bacteria.":[103],"Results:":[104],"amplification":[106],"two":[108],"bands":[109],"512":[111],"bp":[112,115],"249":[114],"genes":[120],"observed,":[122],"respectively.":[123,143],"100%":[127],".The":[128],"0.048":[131],"ng/\u00b5L":[132],"genomic":[134],"determined":[137],"genes,":[142],"Conclusion:":[144],"Duplex-PCR":[145],"method":[147,168],"with":[148],"its":[149],"proper":[152],"feature":[153],"potential":[156],"can":[162],"be":[163],"applied":[164],"as":[165],"routine":[167],"well-equipped":[170],"laboratories":[171],"by":[172],"expert":[173],"technician":[174],"identify":[176],"suspicious":[177],"cases.":[178]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2020-08-24T00:00:00"}