{"id":"https://openalex.org/W305304261","doi":null,"title":"The relative extent and propensity of CD34+ vs. CD34- cells to undergo apoptosis in myelodysplastic marrows.","display_name":"The relative extent and propensity of CD34+ vs. CD34- cells to undergo apoptosis in myelodysplastic marrows.","publication_year":1999,"publication_date":"1999-04-01","ids":{"openalex":"https://openalex.org/W305304261","mag":"305304261","pmid":"https://pubmed.ncbi.nlm.nih.gov/10222652"},"language":"en","primary_location":{"id":"pmid:10222652","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10222652","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of hematology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015365593","display_name":"Suneel Dinkar Mundle","orcid":"https://orcid.org/0000-0003-4867-172X","observed_orcids":["https://orcid.org/0000-0003-4867-172X"]},"institutions":[{"id":"https://openalex.org/I1285301757","display_name":"Rush University Medical Center","ror":"https://ror.org/01j7c0b24","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285301757"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S Mundle","raw_affiliation_strings":["Rush Cancer Institute, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA. smundle@rpslmc.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Rush Cancer Institute, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA. smundle@rpslmc.edu","institution_ids":["https://openalex.org/I1285301757"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025499306","display_name":"Parameswaran Venugopal","orcid":"https://orcid.org/0000-0002-7518-9579","observed_orcids":["https://orcid.org/0000-0002-7518-9579"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Venugopal","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110677291","display_name":"V. Shetty","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"V Shetty","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101668680","display_name":"Anwar Ali","orcid":"https://orcid.org/0000-0001-7994-7317","observed_orcids":["https://orcid.org/0000-0001-7994-7317"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Ali","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071544574","display_name":"Hitesh Chopra","orcid":"https://orcid.org/0000-0001-8867-7603","observed_orcids":["https://orcid.org/0000-0001-8867-7603","https://orcid.org/0000-0002-1406-6903"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Chopra","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"H Handa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Handa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026703756","display_name":"Sheldon H Rose","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Rose","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018449832","display_name":"B. Yifwayimare Mativi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B Y Mativi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109679822","display_name":"S. A. Gregory","orcid":"https://orcid.org/0009-0003-9235-9992","observed_orcids":["https://orcid.org/0009-0003-9235-9992"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S A Gregory","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108534618","display_name":"Harvey D. Preisler","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H D Preisler","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5003413494","display_name":"Abbas Raza","orcid":"https://orcid.org/0000-0003-0359-5647","observed_orcids":["https://orcid.org/0000-0003-0359-5647"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Raza","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.4573,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.88686841,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":92},"biblio":{"volume":"69","issue":"3","first_page":"152","last_page":"9"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/myelodysplastic-syndrome","display_name":"myelodysplastic syndrome","score":0.984000027179718},{"id":"https://openalex.org/keywords/cd34-hematopoietic-progenitor-cells","display_name":"CD34+ hematopoietic progenitor cells","score":0.9559999704360962},{"id":"https://openalex.org/keywords/cd34-hematopoietic-cells","display_name":"CD34+ hematopoietic cells","score":0.9070000052452087},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.5590000152587891},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.7360000014305115},{"id":"https://openalex.org/keywords/apoptotic-index","display_name":"apoptosis index","score":0.5419999957084656},{"id":"https://openalex.org/keywords/in-situ-end-labeling","display_name":"in situ end-labeling","score":0.8199999928474426},{"id":"https://openalex.org/keywords/tunel-assay","display_name":"TUNEL","score":0.8460000157356262},{"id":"https://openalex.org/keywords/nucleic-acid-staining","display_name":"nucleic acid staining","score":0.5559999942779541},{"id":"https://openalex.org/keywords/peripheral-cytopenia","display_name":"peripheral cytopenia","score":0.6359999775886536},{"id":"https://openalex.org/keywords/apoptosis-susceptibility","display_name":"apoptosis susceptibility","score":0.3179999887943268},{"id":"https://openalex.org/keywords/cell-maturation","display_name":"cell maturation","score":0.36500000953674316}],"concepts":[{"id":"https://openalex.org/C10205521","wikidata":"https://www.wikidata.org/wiki/Q4035578","display_name":"CD34","level":3,"score":0.855843186378479},{"id":"https://openalex.org/C196795494","wikidata":"https://www.wikidata.org/wiki/Q1624817","display_name":"TUNEL assay","level":3,"score":0.6311818957328796},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.6281523704528809},{"id":"https://openalex.org/C154113507","wikidata":"https://www.wikidata.org/wiki/Q411149","display_name":"Terminal deoxynucleotidyl transferase","level":4,"score":0.6162297129631042},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.6141334772109985},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.5617203712463379},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.5449503660202026},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.501039981842041},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4934784173965454},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.49139419198036194},{"id":"https://openalex.org/C2780817109","wikidata":"https://www.wikidata.org/wiki/Q954625","display_name":"Myelodysplastic syndromes","level":3,"score":0.48697981238365173},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.4622148871421814},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.43791434168815613},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.4062073826789856},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.35857391357421875},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.34626537561416626},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.22787800431251526},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.17805582284927368},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11563467979431152}],"mesh":[{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001854","descriptor_name":"Bone Marrow Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009190","descriptor_name":"Myelodysplastic Syndromes","qualifier_ui":"Q000503","qualifier_name":"physiopathology","is_major_topic":true},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D018952","descriptor_name":"Antigens, CD34","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D020169","descriptor_name":"Caspases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020287","descriptor_name":"In Situ Nick-End Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053148","descriptor_name":"Caspase 3","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:10222652","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10222652","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of hematology","raw_type":"Journal Article"},{"id":"mag:305304261","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10222652","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":1.0}],"study_designs":[{"id":"https://openalex.org/study-designs/observational-study","display_name":"Observational Study"}],"awards":[{"id":"https://openalex.org/G7032700333","display_name":"CELL AND MOLECULAR BIOLOGY OF ACUTE LEUKEMIA","funder_award_id":"R01CA060086","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G943051260","display_name":"STUDIES OF CELLULAR PROLIFERATION IN MYELOID LEUKEMIAS","funder_award_id":"R01CA060085","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2059492624","https://openalex.org/W2404341986","https://openalex.org/W2591803668","https://openalex.org/W2326501676","https://openalex.org/W2278431283","https://openalex.org/W2361285747","https://openalex.org/W4283165521","https://openalex.org/W4252573792","https://openalex.org/W7055240067","https://openalex.org/W2065628698"],"abstract_inverted_index":{"The":[0,138],"paradox":[1],"of":[2,22,46,70,81,88,91,98,129,144,174,204,232,280,354,365],"peripheral":[3],"cytopenias":[4],"despite":[5],"cellular":[6],"bone":[7],"marrow":[8,185],"(BM)":[9],"observed":[10],"in":[11,77,93,140,146,199,228,248,290,305,350],"myelodysplastic":[12],"syndromes":[13],"(MDS)":[14],"has":[15],"been":[16],"associated":[17],"with":[18,192,225],"excessive":[19],"intramedullary":[20],"apoptosis":[21,53,92,145,197,210],"hematopoietic":[23],"cells.":[24,60,268],"Since":[25],"MDS":[26,74,176,234],"is":[27],"regarded":[28],"as":[29,54,308,384],"a":[30,178,271,281,351,386],"stem":[31],"cell":[32,218],"disorder,":[33],"the":[34,41,89,99,141,171,229,278,287,370,381],"present":[35],"studies":[36,63],"were":[37,64,238,302],"undertaken":[38],"to":[39,51,56,188,221,265,276,310,376],"examine":[40],"relative":[42],"susceptibility":[43],"and":[44,83,95,125,161,167,196,246,257,299,327,359],"propensity":[45,375],"early":[47],"progenitor":[48],"CD34+":[49,94,147,217,236,306,357,371],"cells":[50,103,150,237,242,294,307,312,358,361],"undergo":[52,377],"compared":[55,309],"more":[57],"maturing/matured":[58],"CD34-":[59,96,149,241,311,360,382],"Five":[61],"serial":[62],"performed":[65,181],"on":[66,182,243],"4":[67,175,253],"independent":[68,108],"groups":[69,80],"36":[71],"newly":[72],"diagnosed":[73],"patients.":[75],"First,":[76],"2":[78],"separate":[79],"16":[82],"8":[84,233],"patients":[85],"each,":[86],"measurement":[87],"extent":[90],"fractions":[97],"BM":[100],"aspirate":[101],"mononuclear":[102,355],"was":[104,151,180,219,262,274],"carried":[105,263],"out":[106,264],"using":[107],"biparametric":[109],"flow":[110],"cytometry":[111],"methods,":[112],"CD34":[113,126,190],"labeling/terminal":[114],"deoxynucleotidyl":[115],"transferase-mediated":[116],"dUTP":[117],"nick":[118],"end":[119,201],"labeling":[120,202],"(TUNEL)":[121],"(n":[122,135],"=":[123,136,159,163,322,325,339,342],"16),":[124],"labeling/reduced":[127],"uptake":[128],"nucleic":[130],"acid":[131],"staining":[132],"dye":[133],"LDS751":[134],"8).":[137],"difference":[139],"median":[142],"degrees":[143,364],"vs.":[148,317,334],"not":[152],"statistically":[153],"significant":[154],"by":[155,198],"either":[156],"technique":[157],"(P":[158],"0.583":[160],"P":[162,324,341],"0.674":[164],"for":[165,252,389],"TUNEL":[166],"LDS751,":[168],"respectively).":[169,344],"In":[170,269],"next":[172],"group":[173,231],"patients,":[177,235],"double-labeling":[179],"plastic":[183],"embedded":[184],"biopsy":[186],"sections,":[187],"detect":[189],"antigen":[191],"specific":[193],"monoclonal":[194],"antibody":[195],"situ":[200],"(ISEL)":[203],"fragmented":[205],"DNA.":[206],"Despite":[207],"high":[208],"overall":[209],"(56.2%":[211],"+/-":[212,315,319,332,336],"18.4%),":[213],"only":[214],"an":[215],"occasional":[216],"found":[220],"be":[222],"simultaneously":[223],"labeled":[224,293],"ISEL.":[226],"Finally,":[227],"last":[230],"separated":[239,369],"from":[240],"affinity":[244],"column":[245],"cultured":[247],"serum":[249],"containing":[250],"medium":[251],"hours.":[254],"At":[255],"0-":[256],"4-hour":[258],"time":[259],"points,":[260],"ISEL":[261,292],"label":[266],"apoptotic":[267],"addition,":[270],"fluorometric":[272],"assay":[273],"employed":[275],"estimate":[277],"activity":[279,301],"proapoptotic":[282,390],"enzyme,":[283],"Caspase":[284],"3.":[285],"Both":[286],"net":[288],"increase":[289],"%":[291],"(apoptotic":[295],"index":[296],"or":[297],"AI)":[298],"Caspase-3":[300,328],"significantly":[303],"lower":[304,374],"(AI,":[313],"0.87%":[314],"0.5%":[316],"3.97%":[318],"1.4%,":[320],"n":[321,338],"6,":[323],"0.028":[326],"Units/mg":[329],"protein,":[330],"46.9":[331],"25.0":[333],"71.7":[335],"23.03,":[337],"5,":[340],"0.042,":[343],"We":[345],"conclude":[346],"that":[347],"when":[348],"estimated":[349],"total":[352],"population":[353],"cells,":[356],"show":[362],"comparable":[363],"apoptosis.":[366],"However,":[367],"once":[368],"fraction":[372,383],"demonstrates":[373],"apoptosis,":[378],"thereby":[379],"suggesting":[380],"being":[385],"possible":[387],"source":[388],"signaling.":[391]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}