{"id":"https://openalex.org/W30473567","doi":null,"title":"Isolation of lipid-free C-reactive protein by affinity chromatography.","display_name":"Isolation of lipid-free C-reactive protein by affinity chromatography.","publication_year":1989,"publication_date":"1989-09-01","ids":{"openalex":"https://openalex.org/W30473567","mag":"30473567","pmid":"https://pubmed.ncbi.nlm.nih.gov/2616877"},"language":"en","primary_location":{"id":"pmid:2616877","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2616877","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Revista espanola de fisiologia","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/20.500.12792/5912","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018851146","display_name":"Julio M. Coll","orcid":"https://orcid.org/0000-0001-8496-3493","observed_orcids":["https://orcid.org/0000-0001-8496-3493"]},"institutions":[{"id":"https://openalex.org/I4210106474","display_name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","ror":"https://ror.org/011q66e29","country_code":"ES","type":"funder","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210106474"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"J M Coll","raw_affiliation_strings":["Departamento de Virolog\u00eda, INIA, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Virolog\u00eda, INIA, Madrid, Spain","institution_ids":["https://openalex.org/I4210106474"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5018851146"],"corresponding_institution_ids":["https://openalex.org/I4210106474"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00146638,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"45","issue":"3","first_page":"291","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T14245","display_name":"Biomarkers in Disease Mechanisms","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T14245","display_name":"Biomarkers in Disease Mechanisms","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.04670000076293945,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10394","display_name":"Lipoproteins and Cardiovascular Health","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/c-reactive-protein","display_name":"C-reactive protein","score":1.0},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.8880000114440918},{"id":"https://openalex.org/keywords/affinity-chromatography","display_name":"affinity chromatography","score":0.968999981880188},{"id":"https://openalex.org/keywords/ascitic-fluid","display_name":"ascitic fluid","score":0.8019999861717224}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7284518480300903},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.6564528942108154},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6339123249053955},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.448365181684494},{"id":"https://openalex.org/C2781195455","wikidata":"https://www.wikidata.org/wiki/Q422766","display_name":"C-reactive protein","level":3,"score":0.44176143407821655},{"id":"https://openalex.org/C8985478","wikidata":"https://www.wikidata.org/wiki/Q903485","display_name":"Protein purification","level":2,"score":0.4103214740753174},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2021738588809967},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.11172491312026978},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002097","descriptor_name":"C-Reactive Protein","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008055","descriptor_name":"Lipids","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011232","descriptor_name":"Chemical Precipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:2616877","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2616877","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Revista espanola de fisiologia","raw_type":"Journal Article"},{"id":"pmh:oai:dnet:r2i2________::8603486a65c4bddebb3a42145ffcc4f4","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/20.500.12792/5912","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},{"id":"mag:30473567","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2616877","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:dnet:r2i2________::8603486a65c4bddebb3a42145ffcc4f4","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/20.500.12792/5912","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W2152239270","https://openalex.org/W2557040551","https://openalex.org/W2836284501","https://openalex.org/W2887027097","https://openalex.org/W2614006786","https://openalex.org/W2613065682","https://openalex.org/W2592338220","https://openalex.org/W2776272325","https://openalex.org/W2765218320","https://openalex.org/W2771036474","https://openalex.org/W2610572958","https://openalex.org/W2886103315","https://openalex.org/W2783845982","https://openalex.org/W2886940906","https://openalex.org/W2799449856","https://openalex.org/W2618400336","https://openalex.org/W2066011027","https://openalex.org/W2124338782","https://openalex.org/W2120015206","https://openalex.org/W1990594366","https://openalex.org/W2046672050","https://openalex.org/W1625553530","https://openalex.org/W2039144193","https://openalex.org/W2805996029","https://openalex.org/W2077966352","https://openalex.org/W2110997472","https://openalex.org/W6628910274","https://openalex.org/W1998412506","https://openalex.org/W2005835980","https://openalex.org/W1969130234","https://openalex.org/W2087313998","https://openalex.org/W2604767903","https://openalex.org/W2223535266"],"related_works":["https://openalex.org/W2048620219","https://openalex.org/W29053392","https://openalex.org/W1977575870","https://openalex.org/W2427328353","https://openalex.org/W2040849321","https://openalex.org/W1996207870","https://openalex.org/W2017577616","https://openalex.org/W2083740830","https://openalex.org/W2002611036","https://openalex.org/W2022108426"],"abstract_inverted_index":{"Human":[0],"C-reactive":[1,16,28,50,65],"protein":[2,17,29,51,66],"purification":[3],"has":[4],"been":[5],"hampered":[6],"by":[7,20,32,43,54,74],"its":[8],"association":[9],"with":[10,70,80],"lipids.":[11],"Isolation":[12],"of":[13,57,63,78],"pure":[14,49],"lipid-free":[15,27,48,64],"was":[18,30,52,67],"obtained":[19,31,53,68,73],"a":[21],"three":[22],"step":[23],"procedure.":[24],"First,":[25],"partially":[26],"affinity":[33,55],"chromatography":[34],"from":[35],"ascitic":[36],"fluids;":[37],"second,":[38],"lipid-bound":[39],"proteins":[40],"were":[41],"eliminated":[42],"calcium-dependent":[44],"precipitation;":[45],"and":[46],"third,":[47],"re-chromatography":[56],"the":[58,71,75],"supernatant.":[59],"A":[60],"46-50%":[61],"yield":[62],"compared":[69],"14.7%":[72],"old":[76],"method":[77],"extraction":[79],"lipid":[81],"solvents.":[82]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}