{"id":"https://openalex.org/W3046780658","doi":"https://doi.org/10.1038/s41598-020-69489-8","title":"Gene knockdown via electroporation of short hairpin RNAs in embryos of the marine hydroid Hydractinia symbiolongicarpus","display_name":"Gene knockdown via electroporation of short hairpin RNAs in embryos of the marine hydroid Hydractinia symbiolongicarpus","publication_year":2020,"publication_date":"2020-07-30","ids":{"openalex":"https://openalex.org/W3046780658","doi":"https://doi.org/10.1038/s41598-020-69489-8","mag":"3046780658","pmid":"https://pubmed.ncbi.nlm.nih.gov/32732955","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7393174"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-020-69489-8","doi":"https://doi.org/10.1038/s41598-020-69489-8","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-69489-8","pdf_url":"https://www.nature.com/articles/s41598-020-69489-8.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-020-69489-8.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014997279","display_name":"Gonzalo Quiroga-Artigas","orcid":"https://orcid.org/0000-0001-5954-6794","observed_orcids":["https://orcid.org/0000-0001-5954-6794"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gonzalo Quiroga-Artigas","raw_affiliation_strings":["Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA. gonzalo.artigas@whitney.ufl.edu","Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA. gonzalo.artigas@whitney.ufl.edu","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010231514","display_name":"Alexandrea A. Duscher","orcid":"https://orcid.org/0000-0003-2138-6232","observed_orcids":["https://orcid.org/0000-0003-2138-6232"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexandrea Duscher","raw_affiliation_strings":["Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA","Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061681130","display_name":"Katelyn Lundquist","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katelyn Lundquist","raw_affiliation_strings":["Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA","Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077212516","display_name":"Justin Waletich","orcid":"https://orcid.org/0000-0002-8740-3982","observed_orcids":["https://orcid.org/0000-0002-8740-3982"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Justin Waletich","raw_affiliation_strings":["Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA","Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5020817043","display_name":"Christine E. Schnitzler","orcid":"https://orcid.org/0000-0002-5001-6524","observed_orcids":["https://orcid.org/0000-0002-5001-6524"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Christine E. Schnitzler","raw_affiliation_strings":["Department of Biology, University of Florida, Gainesville, FL, USA. christine.schnitzler@whitney.ufl.edu","Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA. christine.schnitzler@whitney.ufl.edu","Department of Biology, University of Florida, Gainesville, USA","Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Florida, Gainesville, FL, USA. christine.schnitzler@whitney.ufl.edu","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, 32080, USA. christine.schnitzler@whitney.ufl.edu","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Department of Biology, University of Florida, Gainesville, USA","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5014997279","https://openalex.org/A5020817043"],"corresponding_institution_ids":["https://openalex.org/I33213144"],"apc_list":{"value":1870,"currency":"USD","value_usd":1870},"apc_paid":{"value":1870,"currency":"USD","value_usd":1870},"fwci":1.5255,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.8115291,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"10","issue":"1","first_page":"12806","last_page":"12806"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12142","display_name":"Marine Invertebrate Physiology and Ecology","score":0.6137999892234802,"subfield":{"id":"https://openalex.org/subfields/1911","display_name":"Paleontology"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12142","display_name":"Marine Invertebrate Physiology and Ecology","score":0.6137999892234802,"subfield":{"id":"https://openalex.org/subfields/1911","display_name":"Paleontology"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.3716000020503998,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.005200000014156103,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydractinia-symbiolongicarpus","display_name":"Hydractinia symbiolongicarpus","score":0.9089999794960022},{"id":"https://openalex.org/keywords/shrna","display_name":"shRNA","score":0.8629999756813049},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.718999981880188},{"id":"https://openalex.org/keywords/electroporation","display_name":"electroporation","score":0.8429999947547913},{"id":"https://openalex.org/keywords/gene-knockdown","display_name":"gene knockdown","score":0.9760000109672546},{"id":"https://openalex.org/keywords/embryonic-development","display_name":"embryonic development","score":0.6299999952316284},{"id":"https://openalex.org/keywords/nanos2","display_name":"Nanos2","score":0.9309999942779541},{"id":"https://openalex.org/keywords/zygote","display_name":"zygote","score":0.5669999718666077}],"concepts":[{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.8012561798095703},{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.6910884976387024},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6218472719192505},{"id":"https://openalex.org/C190232843","wikidata":"https://www.wikidata.org/wiki/Q1950808","display_name":"Small hairpin RNA","level":4,"score":0.5901378393173218},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5578770637512207},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.5327931046485901},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5308975577354431},{"id":"https://openalex.org/C187226429","wikidata":"https://www.wikidata.org/wiki/Q15260089","display_name":"Hydroid (botany)","level":2,"score":0.503046452999115},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.380141019821167},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3330857455730438}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003063","descriptor_name":"Cnidaria","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":true},{"descriptor_ui":"D003063","descriptor_name":"Cnidaria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004625","descriptor_name":"Embryo, Nonmammalian","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016601","descriptor_name":"RNA-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D047108","descriptor_name":"Embryonic Development","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D055785","descriptor_name":"Gene Knockdown Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1038/s41598-020-69489-8","doi":"https://doi.org/10.1038/s41598-020-69489-8","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-69489-8","pdf_url":"https://www.nature.com/articles/s41598-020-69489-8.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"doi:10.1101/2020.04.07.030296","doi":"https://doi.org/10.1101/2020.04.07.030296","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2020.04.07.030296","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2020/04/08/2020.04.07.030296.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:7393174","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7393174","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:32732955","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32732955","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1038/s41598-020-69489-8","doi":"https://doi.org/10.1038/s41598-020-69489-8","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-69489-8","pdf_url":"https://www.nature.com/articles/s41598-020-69489-8.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2557432840","display_name":"EDGE CT: Enabling cell-specific functional genomics in a colonial animal","funder_award_id":"1923259","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3046780658.pdf","grobid_xml":"https://content.openalex.org/works/W3046780658.grobid-xml"},"referenced_works_count":62,"referenced_works":["https://openalex.org/W189856478","https://openalex.org/W1486227004","https://openalex.org/W1647075334","https://openalex.org/W1951825744","https://openalex.org/W1964647191","https://openalex.org/W1967585260","https://openalex.org/W1976557090","https://openalex.org/W1980017221","https://openalex.org/W1984264742","https://openalex.org/W1987798665","https://openalex.org/W1992289305","https://openalex.org/W2002256585","https://openalex.org/W2009635392","https://openalex.org/W2019679563","https://openalex.org/W2020403596","https://openalex.org/W2046181232","https://openalex.org/W2047793174","https://openalex.org/W2058375243","https://openalex.org/W2073175998","https://openalex.org/W2073821929","https://openalex.org/W2078952624","https://openalex.org/W2086202423","https://openalex.org/W2096641211","https://openalex.org/W2099540110","https://openalex.org/W2106083156","https://openalex.org/W2109473195","https://openalex.org/W2110568699","https://openalex.org/W2119212619","https://openalex.org/W2119563731","https://openalex.org/W2119630670","https://openalex.org/W2124772083","https://openalex.org/W2133898735","https://openalex.org/W2148509233","https://openalex.org/W2148904430","https://openalex.org/W2154661771","https://openalex.org/W2161460309","https://openalex.org/W2170964754","https://openalex.org/W2315702334","https://openalex.org/W2325139807","https://openalex.org/W2397643408","https://openalex.org/W2465310138","https://openalex.org/W2501118822","https://openalex.org/W2530407290","https://openalex.org/W2587597791","https://openalex.org/W2617843580","https://openalex.org/W2624562606","https://openalex.org/W2770730679","https://openalex.org/W2781865485","https://openalex.org/W2790927285","https://openalex.org/W2806734685","https://openalex.org/W2807223659","https://openalex.org/W2810440784","https://openalex.org/W2890399547","https://openalex.org/W2894367246","https://openalex.org/W2949828506","https://openalex.org/W2950662509","https://openalex.org/W2968517987","https://openalex.org/W2971757165","https://openalex.org/W3005757579","https://openalex.org/W3013907986","https://openalex.org/W4205246370","https://openalex.org/W4242968140"],"related_works":["https://openalex.org/W4220904671","https://openalex.org/W2950662509","https://openalex.org/W2806734685","https://openalex.org/W2787050583","https://openalex.org/W6939838291","https://openalex.org/W2066674322","https://openalex.org/W2064606148","https://openalex.org/W2085176759","https://openalex.org/W35731797","https://openalex.org/W6977676344"],"abstract_inverted_index":{"Analyzing":[0],"gene":[1,118,123,185],"function":[2,186],"in":[3,35,157,187],"a":[4,42,174],"broad":[5],"range":[6],"of":[7,17,54,78,82,108,115,132,142,155,180],"research":[8],"organisms":[9],"is":[10],"crucial":[11],"for":[12,75,153,166,177,183],"understanding":[13],"the":[14,55,76,98,167,178],"biological":[15],"functions":[16],"genes":[18],"and":[19,45,92,104,120],"their":[20],"evolution.":[21],"Recent":[22],"studies":[23],"have":[24,40],"shown":[25],"that":[26,66],"short":[27],"hairpin":[28],"RNAs":[29],"(shRNAs)":[30],"can":[31,67],"induce":[32],"gene-specific":[33],"knockdowns":[34,65,114],"two":[36,133],"cnidarian":[37,57],"species.":[38],"We":[39,101,137],"developed":[41],"detailed,":[43],"straightforward,":[44],"scalable":[46],"method":[47,110],"to":[48],"deliver":[49],"shRNAs":[50,79,156],"into":[51,80],"fertilized":[52,83],"eggs":[53,86],"hydrozoan":[56,168],"Hydractinia":[58],"symbiolongicarpus":[59,85,159],"via":[60],"electroporation,":[61],"yielding":[62],"effective":[63,113],"gene-targeted":[64],"last":[68],"throughout":[69],"embryogenesis.":[70],"Our":[71,150],"electroporation":[72,154],"protocol":[73,152],"allows":[74],"transfection":[77,145],"hundreds":[81],"H.":[84,158],"simultaneously":[87],"with":[88],"minimal":[89],"embryo":[90],"death":[91],"no":[93],"long-term":[94],"harmful":[95],"consequences":[96],"on":[97],"developing":[99],"animals.":[100],"show":[102],"RT-qPCR":[103,131],"detailed":[105,151],"phenotypic":[106],"evidence":[107],"our":[109],"successfully":[111],"inducing":[112],"an":[116,121,162],"exogenous":[117],"(eGFP)":[119],"endogenous":[122,135],"(Nanos2),":[124],"as":[125,127,173],"well":[126],"knockdown":[128],"confirmation":[129,141],"by":[130],"other":[134,188],"genes.":[136],"also":[138,171],"provide":[139],"visual":[140],"successful":[143,175],"shRNA":[144],"inside":[146],"embryos":[147,160],"through":[148],"electroporation.":[149],"constitutes":[161],"important":[163],"experimental":[164],"resource":[165],"community":[169],"while":[170],"serving":[172],"model":[176],"development":[179],"similar":[181],"methods":[182],"interrogating":[184],"marine":[189],"invertebrates.":[190]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2020-08-07T00:00:00"}