{"id":"https://openalex.org/W3039199752","doi":"https://doi.org/10.1017/s1431927600033808","title":"Advances in High Resolution Cryo-EM Studies: the Quartenary Structure of the 70S E.Coli Ribosome Revealed at 11.5\u00c5 Resolution.","display_name":"Advances in High Resolution Cryo-EM Studies: the Quartenary Structure of the 70S E.Coli Ribosome Revealed at 11.5\u00c5 Resolution.","publication_year":2000,"publication_date":"2000-08-01","ids":{"openalex":"https://openalex.org/W3039199752","doi":"https://doi.org/10.1017/s1431927600033808","mag":"3039199752"},"language":"en","primary_location":{"id":"doi:10.1017/s1431927600033808","doi":"https://doi.org/10.1017/s1431927600033808","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s1431927600033808","pdf_url":null,"source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017567464","display_name":"Pawel A. Penczek","orcid":"https://orcid.org/0000-0002-2421-0577","observed_orcids":["https://orcid.org/0000-0002-2421-0577"]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P Penczek","raw_affiliation_strings":["Wadsworth Center, Empire State Plaza , Albany, NY, 12201-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Empire State Plaza , Albany, NY, 12201-0509","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053179808","display_name":"ChMT Spahn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"ChMT Spahn","raw_affiliation_strings":["Department of Biomedical Sciences, State University of New York at Albany , NY, 12201-0509","Wadsworth Center, Empire State Plaza , Albany, NY, 12201-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, State University of New York at Albany , NY, 12201-0509","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I392282"]},{"raw_affiliation_string":"Wadsworth Center, Empire State Plaza , Albany, NY, 12201-0509","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076801513","display_name":"Rajendra Kumar Agrawal","orcid":"https://orcid.org/0000-0001-9392-0065","observed_orcids":["https://orcid.org/0000-0001-9392-0065"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]},{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"RK Agrawal","raw_affiliation_strings":["Howard Hughes Medical Institute at Health Research Inc., Wadsworth Center, State University of New York at Albany , NY, 12201-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute at Health Research Inc., Wadsworth Center, State University of New York at Albany , NY, 12201-0509","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I1339417872","https://openalex.org/I1344073410","https://openalex.org/I392282"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090328165","display_name":"Irene S. Gabashvili","orcid":"https://orcid.org/0000-0002-0097-1478","observed_orcids":["https://orcid.org/0000-0002-0097-1478"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"IS Gabashvili","raw_affiliation_strings":["Department of Biomedical Sciences, State University of New York at Albany , NY, 12201-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, State University of New York at Albany , NY, 12201-0509","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I392282"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088775613","display_name":"Robert A. Grassucci","orcid":"https://orcid.org/0000-0002-1437-1537","observed_orcids":["https://orcid.org/0000-0002-1437-1537"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"RA Grassucci","raw_affiliation_strings":["Department of Biomedical Sciences, State University of New York at Albany , NY, 12201-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, State University of New York at Albany , NY, 12201-0509","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I392282"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042892654","display_name":"Joachim Frank","orcid":"https://orcid.org/0000-0001-5449-6943","observed_orcids":["https://orcid.org/0000-0001-5449-6943","https://orcid.org/0000-0002-2442-1112"]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Frank","raw_affiliation_strings":["Wadsworth Center, Empire State Plaza , Albany, NY, 12201-0509"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Empire State Plaza , Albany, NY, 12201-0509","institution_ids":["https://openalex.org/I1339417872"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3554,"currency":"USD","value_usd":3554},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.42602041,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"6","issue":"S2","first_page":"262","last_page":"263"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/70s-ribosome","display_name":"70S ribosome","score":0.9380000233650208},{"id":"https://openalex.org/keywords/escherichia-coli-ribosome","display_name":"Escherichia coli ribosome","score":0.9200000166893005},{"id":"https://openalex.org/keywords/quaternary-structure","display_name":"quaternary structure","score":0.906000018119812},{"id":"https://openalex.org/keywords/cryo-electron-microscopy","display_name":"cryo-electron microscopy","score":0.746999979019165},{"id":"https://openalex.org/keywords/single-particle-analysis","display_name":"single-particle analysis","score":0.9129999876022339},{"id":"https://openalex.org/keywords/low-signal-to-noise-ratio","display_name":"low signal-to-noise ratio","score":0.734000027179718},{"id":"https://openalex.org/keywords/image-processing","display_name":"image processing","score":0.527999997138977},{"id":"https://openalex.org/keywords/high-resolution-structure","display_name":"high-resolution structure","score":0.38499999046325684}],"concepts":[{"id":"https://openalex.org/C164508769","wikidata":"https://www.wikidata.org/wiki/Q5165765","display_name":"Contrast transfer function","level":4,"score":0.7834815382957458},{"id":"https://openalex.org/C65293305","wikidata":"https://www.wikidata.org/wiki/Q7523967","display_name":"Single particle analysis","level":3,"score":0.666321873664856},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.6370193362236023},{"id":"https://openalex.org/C20702342","wikidata":"https://www.wikidata.org/wiki/Q5190506","display_name":"Cryo-electron microscopy","level":2,"score":0.5447985529899597},{"id":"https://openalex.org/C57493831","wikidata":"https://www.wikidata.org/wiki/Q3134666","display_name":"Projection (relational algebra)","level":2,"score":0.5424185395240784},{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.5338842272758484},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.47349128127098083},{"id":"https://openalex.org/C73555534","wikidata":"https://www.wikidata.org/wiki/Q622825","display_name":"Cluster analysis","level":2,"score":0.43188977241516113},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.4191201329231262},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.3581528961658478},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3568311333656311},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.32175570726394653},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.30140843987464905},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.23781561851501465},{"id":"https://openalex.org/C46141821","wikidata":"https://www.wikidata.org/wiki/Q209402","display_name":"Nuclear magnetic resonance","level":1,"score":0.21384340524673462},{"id":"https://openalex.org/C15336307","wikidata":"https://www.wikidata.org/wiki/Q1766051","display_name":"Lens (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C2779345167","wikidata":"https://www.wikidata.org/wiki/Q104541","display_name":"Aerosol","level":2,"score":0.0},{"id":"https://openalex.org/C70638346","wikidata":"https://www.wikidata.org/wiki/Q2913798","display_name":"Spherical aberration","level":3,"score":0.0},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1017/s1431927600033808","doi":"https://doi.org/10.1017/s1431927600033808","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s1431927600033808","pdf_url":null,"source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W2129096064"],"related_works":["https://openalex.org/W2116888204","https://openalex.org/W2569402566","https://openalex.org/W3081570534","https://openalex.org/W2998197581","https://openalex.org/W2536051193","https://openalex.org/W2600520740","https://openalex.org/W2141974515","https://openalex.org/W3122545427","https://openalex.org/W4282015026","https://openalex.org/W2778717545"],"abstract_inverted_index":{"Abstract":[0],"Three-dimensional":[1],"cryo-electron":[2],"microscopy":[3,139],"(EM)":[4],"of":[5,24,40,50,54,129,132,142,147],"macromolecular":[6],"assemblies":[7],"with":[8,76,150],"low":[9,78],"or":[10,70],"nonexistent":[11],"symmetry":[12],"in":[13,29,48,67,83,102],"single-particle":[14],"form":[15],"is":[16],"now":[17],"recognized":[18],"as":[19],"a":[20,151],"powerful,":[21],"legitimate":[22],"method":[23],"structure":[25],"research.":[26],"The":[27,58],"progress":[28],"the":[30,38,51,86,103,130,133],"resolution":[31],"achieved":[32],"has":[33],"always":[34],"been":[35,126],"related":[36],"to":[37,46,74],"introduction":[39],"new":[41,59],"image":[42],"processing":[43],"methods":[44,65],"and":[45,99,117,146],"improvements":[47,128],"understanding":[49],"overall":[52],"strategy":[53],"single":[55],"particle":[56],"analysis.":[57],"techniques":[60],"either":[61],"were":[62,71],"adopted":[63],"from":[64],"developed":[66],"other":[68],"fields,":[69],"specifically":[72],"designed":[73],"deal":[75],"extremely":[77],"Signal-to-Noise":[79],"Ratio":[80],"(SNR)":[81],"data":[82,134],"EM.":[84],"In":[85,122],"former":[87],"category":[88],"we":[89],"can":[90],"place":[91],"multivariate":[92],"statistical":[93],"analysis,":[94],"clustering":[95],"techniques,":[96,98],"correlation":[97],"tomographic":[100],"methods,":[101,106,116],"latter":[104],"alignment":[105],"three-dimensional":[107],"(3D)":[108],"projection":[109],"alignment,":[110],"contrast":[111],"transfer":[112],"function":[113],"(CTF)":[114],"correction":[115],"common-lines":[118],"based":[119],"orientational":[120],"search.":[121],"addition,":[123],"there":[124],"have":[125],"steady":[127],"quality":[131],"by":[135],"seeking":[136],"better":[137],"electron":[138],"conditions":[140],"(usage":[141],"higher":[143],"microscope":[144],"voltage":[145],"microscopes":[148],"equipped":[149],"field":[152],"emission":[153],"gun).":[154]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2020-07-10T00:00:00"}