{"id":"https://openalex.org/W3029761328","doi":"https://doi.org/10.3760/cma.j.issn.0253-2352.2014.03.011","title":"The expression and significance of autophagy in ICMT-induced endplate cartilage degeneration","display_name":"The expression and significance of autophagy in ICMT-induced endplate cartilage degeneration","publication_year":2014,"publication_date":"2014-03-01","ids":{"openalex":"https://openalex.org/W3029761328","doi":"https://doi.org/10.3760/cma.j.issn.0253-2352.2014.03.011","mag":"3029761328"},"language":"en","primary_location":{"id":"mag:3029761328","doi":"https://doi.org/10.3760/cma.j.issn.0253-2352.2014.03.011","is_oa":false,"landing_page_url":"http://rs.yiigle.com/CN12111320143403/429897.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764428191","display_name":"Zhonghua guke zazhi","issn_l":"0253-2352","issn":["0253-2352"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4320800525","host_organization_name":"Chinese Medical Association","host_organization_lineage":["https://openalex.org/P4320800525"],"host_organization_lineage_names":["Chinese Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhonghua guke zazhi","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Yunfei Yu","orcid":"https://orcid.org/0000-0001-7880-5886","observed_orcids":["https://orcid.org/0000-0001-7880-5886"]},"institutions":[{"id":"https://openalex.org/I100357981","display_name":"Wannan Medical College","ror":"https://ror.org/037ejjy86","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I100357981"]},{"id":"https://openalex.org/I4210167381","display_name":"First Affiliated Hospital of Wannan Medical College","ror":"https://ror.org/05wbpaf14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210167381"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yunfei Yu","raw_affiliation_strings":["Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China"],"raw_orcid":"https://orcid.org/0000-0001-7880-5886","affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China","institution_ids":["https://openalex.org/I100357981","https://openalex.org/I4210167381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101947554","display_name":"Hongguang Xu","orcid":"https://orcid.org/0000-0001-5231-3779","observed_orcids":["https://orcid.org/0000-0001-5231-3779"]},"institutions":[{"id":"https://openalex.org/I100357981","display_name":"Wannan Medical College","ror":"https://ror.org/037ejjy86","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I100357981"]},{"id":"https://openalex.org/I4210167381","display_name":"First Affiliated Hospital of Wannan Medical College","ror":"https://ror.org/05wbpaf14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210167381"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hongguang Xu","raw_affiliation_strings":["Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China"],"raw_orcid":"https://orcid.org/0000-0001-5231-3779","affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China","institution_ids":["https://openalex.org/I100357981","https://openalex.org/I4210167381"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Hong Wang","orcid":"https://orcid.org/0000-0003-0477-2908","observed_orcids":["https://orcid.org/0000-0003-0477-2908"]},"institutions":[{"id":"https://openalex.org/I100357981","display_name":"Wannan Medical College","ror":"https://ror.org/037ejjy86","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I100357981"]},{"id":"https://openalex.org/I4210167381","display_name":"First Affiliated Hospital of Wannan Medical College","ror":"https://ror.org/05wbpaf14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210167381"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hong Wang","raw_affiliation_strings":["Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China"],"raw_orcid":"https://orcid.org/0000-0003-0477-2908","affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China","institution_ids":["https://openalex.org/I100357981","https://openalex.org/I4210167381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008881437","display_name":"Wei Yan Zhang","orcid":"https://orcid.org/0000-0002-5111-4097","observed_orcids":["https://orcid.org/0000-0002-5111-4097"]},"institutions":[{"id":"https://openalex.org/I100357981","display_name":"Wannan Medical College","ror":"https://ror.org/037ejjy86","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I100357981"]},{"id":"https://openalex.org/I4210167381","display_name":"First Affiliated Hospital of Wannan Medical College","ror":"https://ror.org/05wbpaf14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210167381"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wei Zhang","raw_affiliation_strings":["Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China"],"raw_orcid":"https://orcid.org/0000-0002-5111-4097","affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China","institution_ids":["https://openalex.org/I100357981","https://openalex.org/I4210167381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002218230","display_name":"Shouliang Xiong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100357981","display_name":"Wannan Medical College","ror":"https://ror.org/037ejjy86","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I100357981"]},{"id":"https://openalex.org/I4210167381","display_name":"First Affiliated Hospital of Wannan Medical College","ror":"https://ror.org/05wbpaf14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210167381"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shouliang Xiong","raw_affiliation_strings":["Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China","institution_ids":["https://openalex.org/I100357981","https://openalex.org/I4210167381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100402917","display_name":"Min Zhang","orcid":"https://orcid.org/0000-0002-4863-9431","observed_orcids":["https://orcid.org/0000-0002-4863-9431"]},"institutions":[{"id":"https://openalex.org/I100357981","display_name":"Wannan Medical College","ror":"https://ror.org/037ejjy86","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I100357981"]},{"id":"https://openalex.org/I4210167381","display_name":"First Affiliated Hospital of Wannan Medical College","ror":"https://ror.org/05wbpaf14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210167381"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Min Zhang","raw_affiliation_strings":["Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China"],"raw_orcid":"https://orcid.org/0000-0002-4863-9431","affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China","institution_ids":["https://openalex.org/I100357981","https://openalex.org/I4210167381"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072700211","display_name":"Cheng-dong Tu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100357981","display_name":"Wannan Medical College","ror":"https://ror.org/037ejjy86","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I100357981"]},{"id":"https://openalex.org/I4210167381","display_name":"First Affiliated Hospital of Wannan Medical College","ror":"https://ror.org/05wbpaf14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210167381"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Cheng-dong Tu","raw_affiliation_strings":["Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China","institution_ids":["https://openalex.org/I100357981","https://openalex.org/I4210167381"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.46835708,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"34","issue":"03","first_page":"317","last_page":"322"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10238","display_name":"Spine and Intervertebral Disc Pathology","score":0.6669999957084656,"subfield":{"id":"https://openalex.org/subfields/2732","display_name":"Orthopedics and Sports Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10238","display_name":"Spine and Intervertebral Disc Pathology","score":0.6669999957084656,"subfield":{"id":"https://openalex.org/subfields/2732","display_name":"Orthopedics and Sports Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":0.271699994802475,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.041099999099969864,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/autophagy","display_name":"autophagy","score":0.9980000257492065},{"id":"https://openalex.org/keywords/endplate-chondrocytes","display_name":"endplate chondrocytes","score":0.8859999775886536},{"id":"https://openalex.org/keywords/intervertebral-disc-degeneration","display_name":"intervertebral disc degeneration","score":0.9639999866485596},{"id":"https://openalex.org/keywords/beclin-1","display_name":"Beclin-1","score":0.8240000009536743},{"id":"https://openalex.org/keywords/lc3","display_name":"LC3","score":0.9990000128746033},{"id":"https://openalex.org/keywords/type-ii-collagen","display_name":"type II collagen","score":0.9390000104904175},{"id":"https://openalex.org/keywords/sox9","display_name":"SOX9","score":0.9440000057220459},{"id":"https://openalex.org/keywords/proteoglycans","display_name":"proteoglycans","score":0.9240000247955322},{"id":"https://openalex.org/keywords/chondrocyte-viability","display_name":"chondrocyte viability","score":0.8669999837875366},{"id":"https://openalex.org/keywords/3-methyladenine","display_name":"3-methyladenine","score":0.9599999785423279}],"concepts":[{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.7605479955673218},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.5771486163139343},{"id":"https://openalex.org/C2780550940","wikidata":"https://www.wikidata.org/wiki/Q502730","display_name":"Cartilage","level":2,"score":0.5622572302818298},{"id":"https://openalex.org/C2780783641","wikidata":"https://www.wikidata.org/wiki/Q738686","display_name":"MTT assay","level":3,"score":0.5153619647026062},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.4749070405960083},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.47199442982673645},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.46619004011154175},{"id":"https://openalex.org/C2779335624","wikidata":"https://www.wikidata.org/wiki/Q407976","display_name":"Proteoglycan","level":3,"score":0.44522446393966675},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.44041121006011963},{"id":"https://openalex.org/C2781013607","wikidata":"https://www.wikidata.org/wiki/Q14877666","display_name":"Type II collagen","level":3,"score":0.42252254486083984},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.36346307396888733},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.3299340009689331},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.3247877359390259},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3029518723487854},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.27288469672203064},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1690513789653778},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10372373461723328},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.058428019285202026}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:3029761328","doi":"https://doi.org/10.3760/cma.j.issn.0253-2352.2014.03.011","is_oa":false,"landing_page_url":"http://rs.yiigle.com/CN12111320143403/429897.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764428191","display_name":"Zhonghua guke zazhi","issn_l":"0253-2352","issn":["0253-2352"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4320800525","host_organization_name":"Chinese Medical Association","host_organization_lineage":["https://openalex.org/P4320800525"],"host_organization_lineage_names":["Chinese Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhonghua guke zazhi","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4246174936","https://openalex.org/W2463550832","https://openalex.org/W2783032909","https://openalex.org/W3047596943","https://openalex.org/W1966558544","https://openalex.org/W1966292479","https://openalex.org/W2319361026","https://openalex.org/W4328052739","https://openalex.org/W2093817246","https://openalex.org/W2528758537"],"abstract_inverted_index":{"Objective":[0],"To":[1],"investigate":[2],"the":[3,8,33,99,124,189,208,214,221,245,251,255,262],"relationship":[4],"between":[5],"autophagy":[6,96,192,252],"and":[7,32,69,76,86,117,138,153,159,168,176,199,240],"endplate":[9,23,100,228,263],"chondrocytes":[10,81,229,264],"degeneration":[11,265],"with":[12,40,220],"intermittent":[13],"cyclic":[14],"mechanical":[15],"tension":[16],"(ICMT)":[17],"in":[18,98,155,179,196,261],"vitro.Methods":[19],"The":[20],"primary":[21],"lumbar":[22],"cartilage":[24,63],"ceils":[25],"of":[26,62,79,113,126,134,149,166,184,191,223,236],"10":[27],"SD":[28],"rats":[29],"were":[30,54,72,82,193],"cultured":[31],"P1":[34],"generation":[35],"was":[36,92,104,211,217],"selected":[37],"to":[38,94,143],"treat":[39],"ICMT":[41,110,226],"(10\uff05,0.5":[42],"Hz)":[43],"for":[44],"0":[45,114],"h,3":[46],"h,12":[47],"h,24":[48],"h,48":[49],"h.The":[50],"cell":[51,215,246,256],"morphology":[52],"changes":[53],"observed":[55,73],"through":[56],"inverted":[57],"phase":[58],"contrast":[59],"microscope.The":[60],"expression":[61,71,78,148,178,235],"marker":[64],"gene":[65],"type":[66,150],"\u2161":[67,151],"collagen,SOX-9":[68],"proteoglycan":[70],"by":[74,84,106,268],"RT-PCR.Beclin-1":[75],"LC3":[77,175,239],"end-plate":[80],"detected":[83,105],"RT-PCR":[85,167],"Western":[87,169],"blotting.The":[88],"monodansylcadaverine":[89],"(MDC)":[90],"staining":[91,186],"used":[93],"detect":[95],"rate":[97],"chondrocytes.The":[101],"cells":[102,112,125,133,209],"activity":[103],"MTT":[107,205],"assay.Results":[108],"After":[109,225],"loading,the":[111,227],"h":[115,119,128,140,157,161,198,201],"group":[116,120,129,137,141,158,162],"3":[118],"kept":[121],"normal":[122],"morphology,but":[123],"12":[127],"presented":[130],"irregular":[131],"shape.The":[132],"24":[135,156,197],"b":[136],"48":[139,160,200],"tended":[142],"assume":[144],"a":[145],"spindle-shaped":[146],"morphology.The":[147],"collagen,proteoglycan":[152],"Sox-9":[154],"decreased":[163,219],"obviously.The":[164],"results":[165,183],"blotting":[170],"showed":[171,187,207],"that":[172,188],"autophagy-related":[173,237],"genes":[174,238],"Beclin-1":[177,241],"time":[180],"dependent":[181],"increasing.The":[182],"MDC":[185],"occurrence":[190],"significantly":[194,212,218,243,249],"increased":[195],"groups":[202],"after":[203],"ICMT,but":[204],"analysis":[206],"viability":[210,216,247],"decreased,and":[213],"stimulation":[222],"3-methyl-adenine.Conclusion":[224],"will":[230],"lose":[231],"their":[232],"phenotype":[233],"gradually.The":[234],"are":[242],"increased,but":[244],"is":[248],"decreased.Inhibiting":[250],"can":[253],"reduce":[254],"survival":[257],"rate.Autophagy":[258],"may":[259],"participate":[260],"process":[266],"induced":[267],"ICMT.\r\n\r\nKey":[269],"words:":[270],"\r\nIntervertebral":[271],"disc":[272],"degeneration;\u00a0":[273],"Chondrocytes;\u00a0":[274],"Autophagy":[275]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2020-06-05T00:00:00"}