{"id":"https://openalex.org/W30285558","doi":null,"title":"Biochemical and genetic characterization of arazyme, an extracellular metalloprotease produced from Serratia proteamaculans HY-3.","display_name":"Biochemical and genetic characterization of arazyme, an extracellular metalloprotease produced from Serratia proteamaculans HY-3.","publication_year":2007,"publication_date":"2007-05-01","ids":{"openalex":"https://openalex.org/W30285558","mag":"30285558","pmid":"https://pubmed.ncbi.nlm.nih.gov/18051297"},"language":"en","primary_location":{"id":"pmid:18051297","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18051297","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microbiology and biotechnology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103678689","display_name":"Jangyul Kwak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I73616290","display_name":"Korea Research Institute of Bioscience and Biotechnology","ror":"https://ror.org/03ep23f07","country_code":"KR","type":"facility","lineage":["https://openalex.org/I2801339556","https://openalex.org/I4210144908","https://openalex.org/I4387152098","https://openalex.org/I73616290"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jangyul Kwak","raw_affiliation_strings":["Insect Resources Research Center Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea Insect BioTech Co., Ltd., Daejeon 305-811, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Insect Resources Research Center Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea Insect BioTech Co., Ltd., Daejeon 305-811, Korea","institution_ids":["https://openalex.org/I73616290"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112507196","display_name":"Ki-Eun Lee","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kieun Lee","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109076198","display_name":"Dong-Ha Shin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dong-Ha Shin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045027396","display_name":"Jin-Soo Maeng","orcid":"https://orcid.org/0000-0002-6005-2991","observed_orcids":["https://orcid.org/0000-0002-6005-2991"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jin-Soo Maeng","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109460759","display_name":"Doo-Sang Park","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Doo-Sang Park","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030724145","display_name":"Hyun Woo Oh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hyun Woo Oh","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076668725","display_name":"Kwang\u2010Hee Son","orcid":"https://orcid.org/0000-0002-3756-5879","observed_orcids":["https://orcid.org/0000-0002-3756-5879"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kwang-Hee Son","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109159990","display_name":"Kyung-Sook Bae","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kyung-Sook Bae","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110025154","display_name":"Ho-Yong Park","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ho-Yong Park","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.2255,"has_fulltext":false,"cited_by_count":35,"citation_normalized_percentile":{"value":0.82969782,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"17","issue":"5","first_page":"761","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.029500000178813934,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/metalloproteinases","display_name":"metalloproteinases","score":0.8939999938011169},{"id":"https://openalex.org/keywords/extracellular-protease","display_name":"extracellular protease","score":0.9300000071525574},{"id":"https://openalex.org/keywords/1-10-phenanthroline-inhibition","display_name":"1,10-phenanthroline inhibition","score":0.871999979019165},{"id":"https://openalex.org/keywords/edta-inhibition","display_name":"EDTA inhibition","score":0.9139999747276306},{"id":"https://openalex.org/keywords/proteolysis","display_name":"proteolysis","score":0.5820000171661377},{"id":"https://openalex.org/keywords/enzyme-characterization","display_name":"enzyme characterization","score":0.3409999907016754}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6676537990570068},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6521855592727661},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.5457824468612671},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5292077660560608},{"id":"https://openalex.org/C55728118","wikidata":"https://www.wikidata.org/wiki/Q425402","display_name":"Metalloproteinase","level":3,"score":0.5256732106208801},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.4855770468711853},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4597622752189636},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.44439947605133057},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.44233065843582153},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.41215717792510986},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2894211411476135}],"mesh":[{"descriptor_ui":"D000418","descriptor_name":"Albumins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002413","descriptor_name":"Cations, Divalent","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D003067","descriptor_name":"Coenzymes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D003094","descriptor_name":"Collagen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004492","descriptor_name":"Edetic Acid","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007633","descriptor_name":"Keratins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008670","descriptor_name":"Metals","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010618","descriptor_name":"Phenanthrolines","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011480","descriptor_name":"Protease Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D012705","descriptor_name":"Serratia","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D012705","descriptor_name":"Serratia","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D013112","descriptor_name":"Spiders","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D041981","descriptor_name":"Gastrointestinal Tract","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D045726","descriptor_name":"Metalloproteases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D045726","descriptor_name":"Metalloproteases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D045726","descriptor_name":"Metalloproteases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D045726","descriptor_name":"Metalloproteases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":4,"locations":[{"id":"pmid:18051297","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18051297","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microbiology and biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:http://repository.kribb.re.kr:8080/:201005/7951","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/201005/7951","pdf_url":null,"source":{"id":"https://openalex.org/S4406922719","display_name":"KRIBB Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"/dspace/upload/original/7074.pdf","raw_type":"Article"},{"id":"pmh:oai:https://repository.kribb.re.kr:201005/7951","doi":null,"is_oa":false,"landing_page_url":"https://repository.kribb.re.kr/handle/201005/7951","pdf_url":null,"source":{"id":"https://openalex.org/S4406922719","display_name":"KRIBB Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"/dspace/upload/original/7074.pdf","raw_type":"Article"},{"id":"mag:30285558","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18051297","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":66,"referenced_works":["https://openalex.org/W2611241332","https://openalex.org/W2168678052","https://openalex.org/W2229695843","https://openalex.org/W2165308914","https://openalex.org/W2118169390","https://openalex.org/W2469052042","https://openalex.org/W2154059556","https://openalex.org/W2775803967","https://openalex.org/W2155256323","https://openalex.org/W2146701863","https://openalex.org/W2062486240","https://openalex.org/W2150456018","https://openalex.org/W2050942542","https://openalex.org/W2105259666","https://openalex.org/W2043951092","https://openalex.org/W2058056848","https://openalex.org/W2163882380","https://openalex.org/W2105550105","https://openalex.org/W2157717016","https://openalex.org/W2071134744","https://openalex.org/W2089158135","https://openalex.org/W1980320803","https://openalex.org/W2695354411","https://openalex.org/W2500482667","https://openalex.org/W2115342159","https://openalex.org/W1985460461","https://openalex.org/W2321424728","https://openalex.org/W2125616358","https://openalex.org/W2504623699","https://openalex.org/W2037994583","https://openalex.org/W2148775441","https://openalex.org/W2080046858","https://openalex.org/W2128294765","https://openalex.org/W2044932116","https://openalex.org/W2126499930","https://openalex.org/W1982967337","https://openalex.org/W2209575903","https://openalex.org/W2164900174","https://openalex.org/W2342440786","https://openalex.org/W2609973112","https://openalex.org/W2161245598","https://openalex.org/W2098914901","https://openalex.org/W2761277905","https://openalex.org/W2133486030","https://openalex.org/W2044612846","https://openalex.org/W2019154801","https://openalex.org/W2094536157","https://openalex.org/W2059212936","https://openalex.org/W2763711876","https://openalex.org/W2553911513","https://openalex.org/W2132047269","https://openalex.org/W2014063304","https://openalex.org/W2204727106","https://openalex.org/W2275216622","https://openalex.org/W2132976876","https://openalex.org/W2008622368","https://openalex.org/W2493678388","https://openalex.org/W2049050243","https://openalex.org/W2290111006","https://openalex.org/W2789521687","https://openalex.org/W2471285324","https://openalex.org/W2157450846","https://openalex.org/W2211631799","https://openalex.org/W2735789192","https://openalex.org/W2060918723","https://openalex.org/W2093248569"],"related_works":["https://openalex.org/W1974704130","https://openalex.org/W2027514070","https://openalex.org/W817604875","https://openalex.org/W4385635465","https://openalex.org/W1756647744","https://openalex.org/W2060516479","https://openalex.org/W7033208664","https://openalex.org/W4399922521","https://openalex.org/W2050857190","https://openalex.org/W2166759385"],"abstract_inverted_index":{"Serratia":[0],"proteamaculans":[1],"HY-3":[2],"isolated":[3,127],"from":[4,165],"the":[5,46,74,86,93,97,101,124,149,160,183],"digestive":[6],"tract":[7],"of":[8,22,45,60,70,76,100,159,163],"a":[9,57,89,129,173],"spider":[10],"produces":[11],"an":[12,18],"extracellular":[13],"protease":[14,54,111,175],"named":[15],"arazyme,":[16],"with":[17,92,157],"estimated":[19],"molecular":[20],"mass":[21],"51.5":[23],"kDa.":[24],"The":[25,53,68,120,144],"purified":[26],"enzyme":[27,87,102],"was":[28,103,126,177],"characterized":[29,42],"as":[30,80,128],"having":[31],"high":[32],"activities":[33,72],"at":[34],"wide":[35],"pH":[36],"and":[37,66,82,118,140],"temperature":[38],"ranges.":[39],"We":[40],"further":[41],"biochemical":[43],"features":[44],"enzymatic":[47,71],"reactions":[48],"under":[49],"various":[50],"reaction":[51],"conditions.":[52],"efficiently":[55],"hydrolyzed":[56],"broad":[58],"range":[59],"protein":[61],"substrates":[62],"including":[63],"albumin,":[64],"keratin,":[65],"collagen.":[67],"dependence":[69],"on":[73],"presence":[75],"metal":[77],"ions":[78],"such":[79],"calcium":[81],"zinc":[83],"indicated":[84],"that":[85,96,148],"is":[88],"metalloprotease,":[90],"together":[91],"previous":[94],"observation":[95],"proteolytic":[98],"activity":[99],"not":[104],"inhibited":[105,115],"by":[106,116],"aspartate,":[107],"cysteine,":[108],"or":[109],"serine":[110],"inhibitors,":[112],"but":[113],"strongly":[114],"1,10-phenanthroline":[117],"EDTA.":[119],"araA":[121,184],"gene":[122,170],"encoding":[123,172],"exoprotease":[125],"5.6":[130],"kb":[131],"BamHl":[132],"fragment":[133],"after":[134],"PCR":[135],"amplification":[136],"using":[137],"degenerate":[138],"primers":[139],"subsequent":[141],"Southern":[142],"hybridization.":[143],"nucleotide":[145],"sequence":[146],"revealed":[147],"deduced":[150],"amino":[151],"acid":[152],"sequences":[153],"shared":[154],"extensive":[155],"similarity":[156],"those":[158],"serralysin":[161],"family":[162],"metalloproteases":[164],"other":[166],"enteric":[167],"bacteria.":[168],"A":[169],"(inh)":[171],"putative":[174],"inhibitor":[176],"also":[178],"identified":[179],"immediately":[180],"adjacent":[181],"to":[182],"structural":[185],"gene.":[186]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}