{"id":"https://openalex.org/W3026328229","doi":null,"title":"Visualisation of altered replication dynamics during the S phase checkpoint response to DNA damage in human cells","display_name":"Visualisation of altered replication dynamics during the S phase checkpoint response to DNA damage in human cells","publication_year":2004,"publication_date":"2004-01-01","ids":{"openalex":"https://openalex.org/W3026328229","mag":"3026328229"},"language":"en","primary_location":{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:10097644","doi":null,"is_oa":false,"landing_page_url":"https://discovery.ucl.ac.uk/id/eprint/10097644/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021262946","display_name":"Catherine J. Merrick","orcid":"https://orcid.org/0000-0001-7583-2176","observed_orcids":["https://orcid.org/0000-0001-7583-2176"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Catherine J. Merrick","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7583-2176","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5021262946"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/intra-s-phase-checkpoint","display_name":"S-phase checkpoint","score":0.8619999885559082},{"id":"https://openalex.org/keywords/dna-damage-response","display_name":"DNA damage response","score":0.8930000066757202},{"id":"https://openalex.org/keywords/dna-replication","display_name":"DNA replication","score":0.7129999995231628},{"id":"https://openalex.org/keywords/replication-fork-stalling","display_name":"replication fork stalling","score":0.9520000219345093},{"id":"https://openalex.org/keywords/origin-firing","display_name":"origin firing","score":0.9070000052452087},{"id":"https://openalex.org/keywords/ionizing-radiation","display_name":"ionizing radiation","score":0.9660000205039978},{"id":"https://openalex.org/keywords/ultraviolet-radiation","display_name":"ultraviolet radiation","score":0.6970000267028809},{"id":"https://openalex.org/keywords/alkylating-agents","display_name":"alkylating agents","score":0.9990000128746033},{"id":"https://openalex.org/keywords/nucleotide-depletion","display_name":"nucleotide depletion","score":0.9829999804496765},{"id":"https://openalex.org/keywords/atm","display_name":"ATM","score":0.6980000138282776},{"id":"https://openalex.org/keywords/attenuated-total-reflectance","display_name":"ATR","score":0.9990000128746033},{"id":"https://openalex.org/keywords/chk1","display_name":"Chk1","score":0.9980000257492065},{"id":"https://openalex.org/keywords/chk2","display_name":"Chk2","score":0.9860000014305115},{"id":"https://openalex.org/keywords/checkpoint-kinase","display_name":"checkpoint kinase","score":0.6840000152587891},{"id":"https://openalex.org/keywords/human-cells","display_name":"human cells","score":0.7850000262260437}],"concepts":[{"id":"https://openalex.org/C54086632","wikidata":"https://www.wikidata.org/wiki/Q5512671","display_name":"G2-M DNA damage checkpoint","level":5,"score":0.7172361016273499},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.673412561416626},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.6530661582946777},{"id":"https://openalex.org/C40523978","wikidata":"https://www.wikidata.org/wiki/Q14675","display_name":"Fork (system call)","level":2,"score":0.6129628419876099},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5812473893165588},{"id":"https://openalex.org/C50001416","wikidata":"https://www.wikidata.org/wiki/Q17861750","display_name":"CHEK1","level":5,"score":0.5658711791038513},{"id":"https://openalex.org/C35195891","wikidata":"https://www.wikidata.org/wiki/Q2289513","display_name":"S phase","level":5,"score":0.5544129610061646},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5029105544090271},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44718655943870544},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.41741040349006653},{"id":"https://openalex.org/C105696609","wikidata":"https://www.wikidata.org/wiki/Q1068809","display_name":"Cell cycle checkpoint","level":4,"score":0.3871192932128906},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38271310925483704},{"id":"https://openalex.org/C98086919","wikidata":"https://www.wikidata.org/wiki/Q118019","display_name":"Eukaryotic DNA replication","level":4,"score":0.36373165249824524},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3233029246330261},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.2996610999107361},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.20203563570976257},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.13536059856414795},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:10097644","doi":null,"is_oa":false,"landing_page_url":"https://discovery.ucl.ac.uk/id/eprint/10097644/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":"Thesis"},{"id":"mag:3026328229","doi":null,"is_oa":false,"landing_page_url":"https://discovery.ucl.ac.uk/id/eprint/10097644/","pdf_url":null,"source":{"id":"https://openalex.org/S4306508231","display_name":"Doctoral thesis, UCL (University College London).","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.428600013256073}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7016056357","https://openalex.org/W2024337788","https://openalex.org/W2150937729","https://openalex.org/W7095882393","https://openalex.org/W2950862121","https://openalex.org/W2166414761","https://openalex.org/W2741359974","https://openalex.org/W2046005298","https://openalex.org/W7099120171","https://openalex.org/W2055604116"],"abstract_inverted_index":{"Eukaryotic":[0],"cells":[1,27,107],"respond":[2],"to":[3,108,116,129,150,154,172,187,218,250],"DNA":[4,23,59,69,251],"damage":[5,188,252],"within":[6],"S":[7,105],"phase":[8,13,106],"by":[9,57,92,189,199],"activating":[10],"an":[11],"intra-S":[12],"checkpoint;":[14],"a":[15,31,44,75,113,136,142,151,208,240],"response":[16,186,249],"which":[17,58],"includes":[18],"reducing":[19],"the":[20,55,90,178,200,224,244,247,258],"rate":[21,124,144],"of":[22,34,39,48,78,80,96,138,145,221,226,231,243,246,262,265],"synthesis.":[24],"In":[25,51],"yeast":[26],"this":[28,216,237,266],"occurs":[29],"via":[30],"checkpoint-dependent":[32],"inhibition":[33],"origin":[35,84,117,155,168,174],"firing":[36,85,118,175],"and":[37,86,141,165,183,191,202,234,256],"stabilisation":[38],"ongoing":[40],"forks,":[41],"together":[42],"with":[43],"checkpoint-":[45],"independent":[46,220,230],"slowing":[47,137,225],"fork":[49,82,87,123,126,139,146,161,213],"movement.":[50],"higher":[52],"eukaryotes,":[53],"however,":[54],"mechanism":[56],"synthesis":[60],"is":[61,63,229],"reduced":[62],"less":[64],"clear.":[65],"This":[66],"work":[67,238],"describes":[68],"fibre":[70],"labelling":[71],"strategies":[72],"that":[73,103],"offer":[74],"quantitative":[76],"assessment":[77],"rates":[79],"replication":[81,99,227,248],"movement,":[83],"stalling":[88,147,164,214],"throughout":[89],"genome":[91],"examining":[93],"large":[94],"numbers":[95],"individually":[97],"labelled":[98],"forks.":[100],"It":[101],"shows":[102],"exposing":[104],"ionising":[109],"radiation":[110],"(IR)":[111],"induces":[112],"transient":[114],"block":[115,153,171],"but":[119,215],"does":[120],"not":[121],"affect":[122],"or":[125,132],"stalling.":[127],"Exposure":[128],"alkylating":[130],"agents":[131],"UV":[133,192],"light":[134],"causes":[135],"movement":[140],"high":[143],"in":[148,185,210,253],"addition":[149],"sustained":[152],"firing.":[156,169],"Nucleotide":[157],"depletion":[158],"also":[159,206],"reduces":[160],"rate,":[162],"increases":[163],"suppresses":[166],"new":[167,173],"The":[170],"depends":[176],"on":[177],"central":[179],"checkpoint":[180,260],"kinases":[181],"ATM":[182],"ATR":[184,205,233],"IR":[190],"respectively.":[193],"Both":[194],"responses":[195],"are":[196],"transduced":[197],"jointly":[198],"CHK1":[201],"CHK2":[203],"kinases.":[204],"has":[207],"role":[209],"preventing":[211],"irreversible":[212],"appears":[217],"be":[219],"CHK1.":[222,235],"Finally,":[223],"forks":[228],"both":[232],"Thus,":[236],"provides":[239],"detailed":[241],"picture":[242],"mechanics":[245],"human":[254],"cells,":[255],"clarifies":[257],"relative":[259],"dependencies":[261],"each":[263],"aspect":[264],"response.":[267]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2020-05-29T00:00:00"}