{"id":"https://openalex.org/W3025682174","doi":"https://doi.org/10.1016/j.mec.2020.e00129","title":"Protein engineering strategies for microbial production of isoprenoids","display_name":"Protein engineering strategies for microbial production of isoprenoids","publication_year":2020,"publication_date":"2020-05-15","ids":{"openalex":"https://openalex.org/W3025682174","doi":"https://doi.org/10.1016/j.mec.2020.e00129","mag":"3025682174","pmid":"https://pubmed.ncbi.nlm.nih.gov/32612930","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7322351"},"language":"en","primary_location":{"id":"doi:10.1016/j.mec.2020.e00129","doi":"https://doi.org/10.1016/j.mec.2020.e00129","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mec.2020.e00129","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2214030119300501/pdf","source":{"id":"https://openalex.org/S2898246255","display_name":"Metabolic Engineering Communications","issn_l":"2214-0301","issn":["2214-0301"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","oarl","poland-70"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering Communications","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.sciencedirect.com/science/article/pii/S2214030119300501/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015298659","display_name":"\u0393\u03b5\u03ce\u03c1\u03b3\u03b9\u03bf\u03c2 \u0394\u03b1\u03bb\u03ad\u03c4\u03bf\u03c2","orcid":"https://orcid.org/0000-0002-1636-6424","observed_orcids":["https://orcid.org/0000-0002-1636-6424"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Georgios Daletos","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009763658","display_name":"Gregory N Stephanopoulos","orcid":"https://orcid.org/0000-0001-6909-4568","observed_orcids":["https://orcid.org/0000-0001-6909-4568"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gregory Stephanopoulos","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5009763658"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":1800,"currency":"USD","value_usd":1800},"apc_paid":{"value":1800,"currency":"USD","value_usd":1800},"fwci":0.2393,"has_fulltext":true,"cited_by_count":22,"citation_normalized_percentile":{"value":0.19959619,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"11","issue":null,"first_page":"e00129","last_page":"e00129"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.6646000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.6646000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.2996000051498413,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.020800000056624413,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/terpenoids","display_name":"terpenoids","score":0.9480000138282776},{"id":"https://openalex.org/keywords/protein-engineering","display_name":"protein engineering","score":0.9610000252723694},{"id":"https://openalex.org/keywords/microbial-production","display_name":"microbial production","score":0.6690000295639038},{"id":"https://openalex.org/keywords/terpene-synthase","display_name":"terpene synthase","score":1.0},{"id":"https://openalex.org/keywords/cytochrome-p450","display_name":"cytochrome P450","score":0.9869999885559082},{"id":"https://openalex.org/keywords/enzyme-stability","display_name":"enzyme stability","score":0.6779999732971191},{"id":"https://openalex.org/keywords/multienzyme-systems","display_name":"multienzyme systems","score":0.7459999918937683},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.6690000295639038},{"id":"https://openalex.org/keywords/natural-product-biosynthesis","display_name":"natural product biosynthesis","score":0.652999997138977}],"concepts":[{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.7882446050643921},{"id":"https://openalex.org/C206103511","wikidata":"https://www.wikidata.org/wiki/Q426694","display_name":"Terpenoid","level":2,"score":0.7432374954223633},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.7288727164268494},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.6207733750343323},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.6134728789329529},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5926481485366821},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5786609649658203},{"id":"https://openalex.org/C44937612","wikidata":"https://www.wikidata.org/wiki/Q212364","display_name":"Terpene","level":2,"score":0.5029258131980896},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.47558319568634033},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4560946226119995},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3750191032886505},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.37125128507614136},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13002648949623108},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.10722482204437256}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1016/j.mec.2020.e00129","doi":"https://doi.org/10.1016/j.mec.2020.e00129","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mec.2020.e00129","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2214030119300501/pdf","source":{"id":"https://openalex.org/S2898246255","display_name":"Metabolic Engineering Communications","issn_l":"2214-0301","issn":["2214-0301"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","oarl","poland-70"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering Communications","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7322351","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7322351","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metab Eng Commun","raw_type":"Text"},{"id":"pmid:32612930","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32612930","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic engineering communications","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:ba0bd10274084865abd5cc24ebad508a","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/ba0bd10274084865abd5cc24ebad508a","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Metabolic Engineering Communications, Vol 11, Iss , Pp e00129- (2020)","raw_type":"article"},{"id":"pmh:oai:dspace.mit.edu:1721.1/135314","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1721.1/135314","pdf_url":null,"source":{"id":"https://openalex.org/S4306400425","display_name":"DSpace@MIT (Massachusetts Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I63966007","host_organization_name":"Massachusetts Institute of Technology","host_organization_lineage":["https://openalex.org/I63966007"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Elsevier","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1016/j.mec.2020.e00129","doi":"https://doi.org/10.1016/j.mec.2020.e00129","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mec.2020.e00129","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2214030119300501/pdf","source":{"id":"https://openalex.org/S2898246255","display_name":"Metabolic Engineering Communications","issn_l":"2214-0301","issn":["2214-0301"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","oarl","poland-70"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering Communications","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2622844495","display_name":null,"funder_award_id":"DA 2120/1-1","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G3102860443","display_name":"Yarrowia lipolytica als geeignete Plattform f\u00fcr die heterologe Produktion von Tanshinonen","funder_award_id":"405772767","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3025682174.pdf","grobid_xml":"https://content.openalex.org/works/W3025682174.grobid-xml"},"referenced_works_count":91,"referenced_works":["https://openalex.org/W335986809","https://openalex.org/W1434800253","https://openalex.org/W1475401685","https://openalex.org/W1579030151","https://openalex.org/W1830880602","https://openalex.org/W1939495881","https://openalex.org/W1964478423","https://openalex.org/W1966005117","https://openalex.org/W1968212788","https://openalex.org/W1973494721","https://openalex.org/W1978806418","https://openalex.org/W1979964161","https://openalex.org/W1987657458","https://openalex.org/W1992306108","https://openalex.org/W1994402710","https://openalex.org/W1996629685","https://openalex.org/W1999771206","https://openalex.org/W2001602167","https://openalex.org/W2003117374","https://openalex.org/W2012948311","https://openalex.org/W2012968714","https://openalex.org/W2014177423","https://openalex.org/W2021842042","https://openalex.org/W2028117464","https://openalex.org/W2035637936","https://openalex.org/W2036428713","https://openalex.org/W2042680126","https://openalex.org/W2048970513","https://openalex.org/W2050146233","https://openalex.org/W2055194955","https://openalex.org/W2058927469","https://openalex.org/W2072499238","https://openalex.org/W2077787026","https://openalex.org/W2077837797","https://openalex.org/W2079347688","https://openalex.org/W2079574042","https://openalex.org/W2090268157","https://openalex.org/W2092122738","https://openalex.org/W2094931921","https://openalex.org/W2107844301","https://openalex.org/W2108926990","https://openalex.org/W2117148991","https://openalex.org/W2119020076","https://openalex.org/W2121603561","https://openalex.org/W2123945540","https://openalex.org/W2124838044","https://openalex.org/W2137448913","https://openalex.org/W2139132507","https://openalex.org/W2139705098","https://openalex.org/W2147516046","https://openalex.org/W2150148139","https://openalex.org/W2159148582","https://openalex.org/W2159848960","https://openalex.org/W2161460144","https://openalex.org/W2162943526","https://openalex.org/W2171419407","https://openalex.org/W2174272296","https://openalex.org/W2174665123","https://openalex.org/W2207046140","https://openalex.org/W2223798378","https://openalex.org/W2294225971","https://openalex.org/W2298930018","https://openalex.org/W2321424493","https://openalex.org/W2332641124","https://openalex.org/W2335520960","https://openalex.org/W2418420146","https://openalex.org/W2472470407","https://openalex.org/W2511831309","https://openalex.org/W2517733084","https://openalex.org/W2541859010","https://openalex.org/W2555293666","https://openalex.org/W2574601093","https://openalex.org/W2580403151","https://openalex.org/W2604438798","https://openalex.org/W2623250387","https://openalex.org/W2625682243","https://openalex.org/W2787182712","https://openalex.org/W2900473000","https://openalex.org/W2903829928","https://openalex.org/W2906201256","https://openalex.org/W2909155258","https://openalex.org/W2911459582","https://openalex.org/W2914012452","https://openalex.org/W2937027327","https://openalex.org/W2947525207","https://openalex.org/W2952163063","https://openalex.org/W2969469154","https://openalex.org/W2973533038","https://openalex.org/W4234714728","https://openalex.org/W6628358085","https://openalex.org/W6726258979"],"related_works":["https://openalex.org/W3023757646","https://openalex.org/W1983727564","https://openalex.org/W3080968229","https://openalex.org/W201883925","https://openalex.org/W2323395531","https://openalex.org/W2944543437","https://openalex.org/W2143814332","https://openalex.org/W182946326","https://openalex.org/W1598282860","https://openalex.org/W2167275225"],"abstract_inverted_index":{"Isoprenoids":[0],"comprise":[1],"one":[2],"of":[3,9,19,28,32,46,63,98,108,121,139,146,153],"the":[4,25,61,91,102,119,136],"most":[5],"chemically":[6],"diverse":[7],"family":[8],"natural":[10,103],"products":[11],"with":[12,69],"high":[13],"commercial":[14],"interest.":[15],"The":[16,43],"structural":[17],"diversity":[18],"isoprenoids":[20],"is":[21,52],"mainly":[22],"due":[23],"to":[24,54,89,160],"modular":[26,106],"activity":[27],"three":[29],"distinct":[30],"classes":[31],"enzymes,":[33,71],"including":[34],"prenyl":[35],"diphosphate":[36],"synthases,":[37,39],"terpene":[38],"and":[40,77,95,105,118,131,150],"cytochrome":[41],"P450s.":[42],"heterologous":[44],"expression":[45,78],"these":[47,82],"enzymes":[48,110],"in":[49],"microbial":[50,144],"systems":[51],"suggested":[53],"be":[55],"a":[56,157],"promising":[57],"sustainable":[58],"way":[59],"for":[60,115,135],"production":[62,120,145],"isoprenoids.":[64,148],"Several":[65],"limitations":[66],"are":[67,163],"associated":[68],"native":[70],"such":[72],"as":[73,156],"low":[74],"stability,":[75],"activity,":[76,93],"profiles.":[79],"To":[80],"address":[81],"challenges,":[83],"protein":[84,154],"engineering":[85,141,155,162],"has":[86],"been":[87],"applied":[88],"improve":[90],"catalytic":[92],"selectivity,":[94],"substrate":[96],"turnover":[97],"enzymes.":[99],"In":[100,124],"addition,":[101],"promiscuity":[104],"fashion":[107],"isoprenoid":[109],"render":[111],"them":[112],"excellent":[113],"targets":[114],"combinatorial":[116],"studies":[117],"new-to-nature":[122],"metabolites.":[123],"this":[125],"review,":[126],"we":[127],"discuss":[128],"key":[129],"individual":[130],"multienzyme":[132],"level":[133],"strategies":[134],"successful":[137],"implementation":[138],"enzyme":[140],"towards":[142],"efficient":[143],"high-value":[147],"Challenges":[149],"future":[151],"directions":[152],"complementary":[158],"strategy":[159],"metabolic":[161],"likewise":[164],"outlined.":[165]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2020-05-21T00:00:00"}
