{"id":"https://openalex.org/W3021046005","doi":"https://doi.org/10.1002/0471142727.mb25b06s80","title":"Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis","display_name":"Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis","publication_year":2007,"publication_date":"2007-10-01","ids":{"openalex":"https://openalex.org/W3021046005","doi":"https://doi.org/10.1002/0471142727.mb25b06s80","mag":"3021046005","pmid":"https://pubmed.ncbi.nlm.nih.gov/18265400"},"language":"en","primary_location":{"id":"doi:10.1002/0471142727.mb25b06s80","doi":"https://doi.org/10.1002/0471142727.mb25b06s80","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471142727.mb25b06s80","pdf_url":null,"source":{"id":"https://openalex.org/S2755386604","display_name":"Current Protocols in Molecular Biology","issn_l":"1934-3639","issn":["1934-3639","1934-3647"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Molecular Biology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077721872","display_name":"Seth Blackshaw","orcid":"https://orcid.org/0000-0002-1338-8476","observed_orcids":["https://orcid.org/0000-0002-1338-8476"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Seth Blackshaw","raw_affiliation_strings":["Johns Hopkins University School of Medicine, Baltimore Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Johns Hopkins University School of Medicine, Baltimore Maryland","institution_ids":["https://openalex.org/I145311948"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055104705","display_name":"Brad St. Croix","orcid":"https://orcid.org/0000-0002-6246-0374","observed_orcids":["https://orcid.org/0000-0002-6246-0374"]},"institutions":[{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brad St. Croix","raw_affiliation_strings":["National Cancer Institute Frederick Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Cancer Institute Frederick Maryland","institution_ids":["https://openalex.org/I4210140884"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014395742","display_name":"Korn\u00e9lia Poly\u00e1k","orcid":"https://orcid.org/0000-0002-5964-0382","observed_orcids":["https://orcid.org/0000-0002-5964-0382"]},"institutions":[{"id":"https://openalex.org/I4210117453","display_name":"Dana-Farber Cancer Institute","ror":"https://ror.org/02jzgtq86","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210117453"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kornelia Polyak","raw_affiliation_strings":["Dana-Farber Cancer Institute Boston Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dana-Farber Cancer Institute Boston Massachusetts","institution_ids":["https://openalex.org/I4210117453"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101950148","display_name":"Jae Bum Kim","orcid":"https://orcid.org/0000-0001-6942-4037","observed_orcids":["https://orcid.org/0000-0001-6942-4037"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jae Bum Kim","raw_affiliation_strings":["Brigham and Women's Hospital Boston Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Brigham and Women's Hospital Boston Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077130141","display_name":"Li Cai","orcid":"https://orcid.org/0000-0003-3344-337X","observed_orcids":["https://orcid.org/0000-0003-3344-337X"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Li Cai","raw_affiliation_strings":["Rutgers University Piscataway New Jersey"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Rutgers University Piscataway New Jersey","institution_ids":["https://openalex.org/I102322142"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5050,"currency":"USD","value_usd":5050},"apc_paid":null,"fwci":0.1324,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.53025129,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"80","issue":"1","first_page":"Unit 25B.6","last_page":"Unit 25B.6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.7109000086784363,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.7109000086784363,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.22579999268054962,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0333000011742115,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/serial-analysis-of-gene-expression","display_name":"serial analysis of gene expression","score":0.9900000095367432},{"id":"https://openalex.org/keywords/sage-tags","display_name":"SAGE tags","score":0.8019999861717224},{"id":"https://openalex.org/keywords/gene-expression-profiling","display_name":"gene expression profiling","score":0.7419999837875366},{"id":"https://openalex.org/keywords/cdna-sequencing","display_name":"cDNA sequencing","score":0.6259999871253967},{"id":"https://openalex.org/keywords/clustering","display_name":"clustering","score":0.6269999742507935},{"id":"https://openalex.org/keywords/interlaboratory-comparison","display_name":"interlaboratory comparison","score":0.5270000100135803}],"concepts":[{"id":"https://openalex.org/C20032557","wikidata":"https://www.wikidata.org/wiki/Q286721","display_name":"Serial analysis of gene expression","level":5,"score":0.9387465119361877},{"id":"https://openalex.org/C2775870830","wikidata":"https://www.wikidata.org/wiki/Q3379200","display_name":"SAGE","level":2,"score":0.9026705026626587},{"id":"https://openalex.org/C198531522","wikidata":"https://www.wikidata.org/wiki/Q485146","display_name":"Sample (material)","level":2,"score":0.558648943901062},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5333296060562134},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.513736367225647},{"id":"https://openalex.org/C58489278","wikidata":"https://www.wikidata.org/wiki/Q1172284","display_name":"Data set","level":2,"score":0.5002310276031494},{"id":"https://openalex.org/C177264268","wikidata":"https://www.wikidata.org/wiki/Q1514741","display_name":"Set (abstract data type)","level":2,"score":0.47926732897758484},{"id":"https://openalex.org/C90559484","wikidata":"https://www.wikidata.org/wiki/Q778379","display_name":"Expression (computer science)","level":2,"score":0.45847299695014954},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4570671319961548},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.43746042251586914},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42423006892204285},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3716086447238922},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.35102489590644836},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.33942851424217224},{"id":"https://openalex.org/C18431079","wikidata":"https://www.wikidata.org/wiki/Q1502169","display_name":"Gene expression profiling","level":4,"score":0.29894688725471497},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.12079134583473206},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.080037921667099},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/0471142727.mb25b06s80","doi":"https://doi.org/10.1002/0471142727.mb25b06s80","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471142727.mb25b06s80","pdf_url":null,"source":{"id":"https://openalex.org/S2755386604","display_name":"Current Protocols in Molecular Biology","issn_l":"1934-3639","issn":["1934-3639","1934-3647"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Molecular Biology","raw_type":"journal-article"},{"id":"pmid:18265400","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18265400","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current protocols in molecular biology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W1657827244","https://openalex.org/W2002208297","https://openalex.org/W2010888033","https://openalex.org/W2033585891","https://openalex.org/W2035307362","https://openalex.org/W2039457028","https://openalex.org/W2049820772","https://openalex.org/W2071038281","https://openalex.org/W2073883677","https://openalex.org/W2094089605","https://openalex.org/W2098350013","https://openalex.org/W2098357168","https://openalex.org/W2117013761","https://openalex.org/W2127938920","https://openalex.org/W2133636765","https://openalex.org/W2133868621","https://openalex.org/W2142070680","https://openalex.org/W2146516445","https://openalex.org/W2151238743","https://openalex.org/W4213265720","https://openalex.org/W4213387068","https://openalex.org/W4249877820","https://openalex.org/W4252521233"],"related_works":["https://openalex.org/W3022623018","https://openalex.org/W4287757934","https://openalex.org/W7099812563","https://openalex.org/W1990419284","https://openalex.org/W2283151646","https://openalex.org/W2168720699","https://openalex.org/W2412757805","https://openalex.org/W2053735404","https://openalex.org/W2559404410","https://openalex.org/W2397839254"],"abstract_inverted_index":{"Serial":[0],"analysis":[1,138],"of":[2,9,12,23,34,63,79,89,139],"gene":[3,64],"expression":[4,65],"(SAGE)":[5],"involves":[6],"the":[7,21,27,42,53,112],"generation":[8],"short":[10,31],"fragments":[11],"DNA,":[13],"or":[14,76],"tags,":[15],"from":[16,70],"a":[17,38,60],"defined":[18,39],"point":[19,40],"in":[20,26,37,41,52,66],"sequence":[22],"all":[24],"cDNAs":[25],"sample":[28,68],"analyzed.":[29],"This":[30],"tag,":[32],"because":[33],"its":[35],"presence":[36],"sequence,":[43],"is":[44,121],"typically":[45],"sufficient":[46],"to":[47,58,99,105,135],"uniquely":[48],"identify":[49],"every":[50],"transcript":[51],"sample.":[54],"SAGE":[55,82,113,133],"allows":[56,96],"one":[57],"generate":[59],"comprehensive":[61],"profile":[62],"any":[67],"desired":[69],"as":[71,73,118],"little":[72],"100,000":[74],"cells":[75],"1":[77],"microg":[78],"total":[80],"RNA.":[81],"generates":[83],"absolute,":[84],"rather":[85],"than":[86],"relative,":[87],"measurements":[88],"RNA":[90],"abundance":[91],"levels,":[92],"and":[93,101,123,142],"this":[94],"fact":[95],"an":[97],"investigator":[98],"readily":[100],"reliably":[102],"compare":[103],"data":[104,114,120],"those":[106],"produced":[107],"by":[108],"other":[109],"laboratories,":[110],"making":[111],"set":[115],"increasingly":[116],"useful":[117],"more":[119],"generated":[122],"shared.":[124],"Software":[125],"tools":[126],"have":[127],"also":[128],"been":[129],"specifically":[130],"adapted":[131],"for":[132],"tags":[134],"allow":[136],"cluster":[137],"both":[140],"public":[141],"user-generated":[143],"data.":[144]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2020-05-13T00:00:00"}