{"id":"https://openalex.org/W3020373377","doi":null,"title":"Effect of heat shock on amino acid metabolism in cultured cowpea cells: Changes in the metabolism of gamma-aminobutyric acid","display_name":"Effect of heat shock on amino acid metabolism in cultured cowpea cells: Changes in the metabolism of gamma-aminobutyric acid","publication_year":1993,"publication_date":"1993-01-01","ids":{"openalex":"https://openalex.org/W3020373377","mag":"3020373377"},"language":"en","primary_location":{"id":"pmh:oai:docs.lib.purdue.edu:dissertations-31111","doi":null,"is_oa":false,"landing_page_url":"https://docs.lib.purdue.edu/dissertations/AAI9334359","pdf_url":null,"source":{"id":"https://openalex.org/S4377196310","display_name":"Purdue e-Pubs (Purdue University System)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801333002","host_organization_name":"Purdue University System","host_organization_lineage":["https://openalex.org/I2801333002"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Theses and Dissertations Available from ProQuest","raw_type":"text"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090793058","display_name":"Kathleen L. Heuss","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Kathleen Lucille Heuss-LaRosa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5090793058"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13142","display_name":"GABA and Rice Research","score":0.5205000042915344,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13142","display_name":"GABA and Rice Research","score":0.5205000042915344,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.35179999470710754,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.02250000089406967,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heat-shock","display_name":"heat shock","score":1.0},{"id":"https://openalex.org/keywords/heat-adaptation","display_name":"heat adaptation","score":0.8420000076293945},{"id":"https://openalex.org/keywords/gaba","display_name":"GABA","score":0.8899999856948853},{"id":"https://openalex.org/keywords/gaba-synthesis","display_name":"GABA synthesis","score":0.8790000081062317},{"id":"https://openalex.org/keywords/glutamic-acid-decarboxylase","display_name":"glutamic acid decarboxylase","score":0.9129999876022339},{"id":"https://openalex.org/keywords/gaba-transaminase","display_name":"GABA transaminase","score":0.9070000052452087},{"id":"https://openalex.org/keywords/gabaculine","display_name":"gabaculine","score":0.9369999766349792},{"id":"https://openalex.org/keywords/amino-acid-metabolism","display_name":"amino acid metabolism","score":0.925000011920929},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.7310000061988831},{"id":"https://openalex.org/keywords/cytoplasmic-acidification","display_name":"cytoplasmic acidification","score":0.8619999885559082},{"id":"https://openalex.org/keywords/protein-synthesis","display_name":"protein synthesis","score":0.4729999899864197}],"concepts":[{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.8312983512878418},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.47557511925697327},{"id":"https://openalex.org/C2781300812","wikidata":"https://www.wikidata.org/wiki/Q178061","display_name":"Shock (circulatory)","level":2,"score":0.4633033871650696},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4492741823196411},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3709610402584076},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.15971606969833374},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.07212617993354797}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:docs.lib.purdue.edu:dissertations-31111","doi":null,"is_oa":false,"landing_page_url":"https://docs.lib.purdue.edu/dissertations/AAI9334359","pdf_url":null,"source":{"id":"https://openalex.org/S4377196310","display_name":"Purdue e-Pubs (Purdue University System)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801333002","host_organization_name":"Purdue University System","host_organization_lineage":["https://openalex.org/I2801333002"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Theses and Dissertations Available from ProQuest","raw_type":"text"},{"id":"mag:3020373377","doi":null,"is_oa":false,"landing_page_url":"https://docs.lib.purdue.edu/cgi/viewcontent.cgi?article=31111&context=dissertations","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1971285509","https://openalex.org/W2284835501","https://openalex.org/W2499956173","https://openalex.org/W3085158513","https://openalex.org/W2085114863","https://openalex.org/W2558921110","https://openalex.org/W1983470248","https://openalex.org/W6884615185","https://openalex.org/W3171621662","https://openalex.org/W1566451495"],"abstract_inverted_index":{"The":[0],"effects":[1,242],"of":[2,32,45,78,90,94,104,116,118,171,206,215,221,243,262],"heat":[3,6,70,122,235],"shock":[4,19,71,236],"and":[5,62,97,102,151,192,212,259],"adaptation":[7],"on":[8],"amino":[9,158,161],"acid":[10,34],"metabolism":[11],"in":[12,25,29,59,75,99,168,209,249,257],"cowpea":[13],"suspension":[14],"cultures":[15],"were":[16,153,166],"studied.":[17],"Heat":[18],"(from":[20],"26":[21,146],"to":[22,56,142,200,253],"42$\\sp\\circ$C)":[23],"resulted":[24],"a":[26,42,178,246],"several-fold":[27],"increase":[28,53,58,248,256],"the":[30,87,100,114,154,169,254],"accumulation":[31],"gamma-aminobutyric":[33],"(GABA)":[35],"within":[36],"4":[37],"hours.":[38],"Experiments":[39],"with":[40],"gabaculine,":[41],"potent":[43],"inhibitor":[44],"GABA":[46,60,65,91,95,133,165,232],"aminotransferases":[47],"(GABA":[48],"AT),":[49],"confirmed":[50],"that":[51,64,107],"this":[52],"is":[54,110],"due":[55,252],"an":[57],"biosynthesis,":[61],"indicated":[63],"utilization":[66,98],"also":[67],"increases.":[68],"However,":[69],"did":[72],"not":[73,111],"result":[74,239],"increased":[76],"levels":[77,117],"GABA:pyruvate":[79],"AT,":[80,82],"GABA:2-oxoglutarate":[81],"or":[83,147,224],"glutamate":[84,150,182],"decarboxylase":[85],"(GAD),":[86],"major":[88],"enzyme":[89,201,222],"biosynthesis.":[92],"Measurements":[93],"biosynthesis":[96,233],"presence":[101,170],"absence":[103],"cycloheximide":[105],"suggested":[106],"protein":[108],"synthesis":[109],"required":[112],"for":[113,181],"maintenance":[115],"these":[119],"enzymes":[120],"during":[121,234],"shock.":[123],"In":[124],"38$\\sp\\circ$C-adapted":[125],"cells,":[126],"GAD":[127,173,207],"level":[128],"was":[129,134,140],"decreased,":[130],"even":[131],"though":[132],"somewhat":[135],"increased.":[136],"When":[137],"($\\sp{15}$N)":[138],"-GABA":[139],"fed":[141],"cells":[143,176],"incubated":[144],"at":[145],"42$\\sp\\circ$C,":[148],"alanine,":[149],"aspartate":[152],"most":[155],"heavily":[156],"labeled":[157,167],"acids.":[159],"No":[160],"acids":[162],"other":[163],"than":[164],"gabaculine.":[172],"from":[174,202,240],"heat-shocked":[175],"had":[177],"K$\\sb{\\rm":[179],"m}$":[180],"($\\sim$3.5":[183],"mM),":[184],"activity":[185,193,208],"versus":[186,194],"pH":[187],"curve":[188,196],"(pH":[189],"optimum":[190],"$\\sim$5.1),":[191],"temperature":[195],"(Q$\\sb{10}\\":[197],"\\sim2$)":[198],"identical":[199],"control":[203],"cells.":[204],"Comparison":[205],"extracts":[210],"before":[211],"after":[213],"removal":[214],"small":[216],"solutes":[217],"revealed":[218],"no":[219],"evidence":[220],"activation":[223],"inhibition":[225],"by":[226],"low":[227],"molecular":[228],"weight":[229],"effectors.":[230],"Increased":[231],"could":[237],"possibly":[238],"combined":[241],"cytoplasmic":[244],"acidification,":[245],"3-fold":[247],"reaction":[250],"velocity":[251],"16$\\sp\\circ$C":[255],"temperature,":[258],"altered":[260],"compartmentation":[261],"glutamate.":[263]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2020-05-01T00:00:00"}