{"id":"https://openalex.org/W3020041465","doi":"https://doi.org/10.1093/nar/gkaa272","title":"Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs","display_name":"Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs","publication_year":2020,"publication_date":"2020-04-07","ids":{"openalex":"https://openalex.org/W3020041465","doi":"https://doi.org/10.1093/nar/gkaa272","mag":"3020041465","pmid":"https://pubmed.ncbi.nlm.nih.gov/32329776","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7261169"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkaa272","doi":"https://doi.org/10.1093/nar/gkaa272","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkaa272","pdf_url":"https://academic.oup.com/nar/article-pdf/48/10/5624/33326207/gkaa272.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/nar/article-pdf/48/10/5624/33326207/gkaa272.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076793274","display_name":"Thomas L. Jacobsen","orcid":"https://orcid.org/0000-0003-4453-7670","observed_orcids":["https://orcid.org/0000-0003-4453-7670"]},"institutions":[{"id":"https://openalex.org/I137902535","display_name":"North Carolina State University","ror":"https://ror.org/04tj63d06","country_code":"US","type":"education","lineage":["https://openalex.org/I137902535"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas Jacobsen","raw_affiliation_strings":["Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA","institution_ids":["https://openalex.org/I137902535"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021196091","display_name":"Fani Ttofali","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137902535","display_name":"North Carolina State University","ror":"https://ror.org/04tj63d06","country_code":"US","type":"education","lineage":["https://openalex.org/I137902535"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fani Ttofali","raw_affiliation_strings":["Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA","institution_ids":["https://openalex.org/I137902535"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101727502","display_name":"Chunyu Liao","orcid":"https://orcid.org/0000-0002-5563-3513","observed_orcids":["https://orcid.org/0000-0002-5563-3513"]},"institutions":[{"id":"https://openalex.org/I4210124929","display_name":"Helmholtz Centre for Infection Research","ror":"https://ror.org/03d0p2685","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I4210124929"]},{"id":"https://openalex.org/I4210125858","display_name":"Helmholtz Institute for RNA-based Infection Research","ror":"https://ror.org/02a98s891","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I4210124929","https://openalex.org/I4210125858"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Chunyu Liao","raw_affiliation_strings":["Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080 W\u00fcrzburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080 W\u00fcrzburg, Germany","institution_ids":["https://openalex.org/I4210124929","https://openalex.org/I4210125858"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087109741","display_name":"Srinivas Manchalu","orcid":"https://orcid.org/0000-0003-3641-2827","observed_orcids":["https://orcid.org/0000-0003-3641-2827"]},"institutions":[{"id":"https://openalex.org/I4210124929","display_name":"Helmholtz Centre for Infection Research","ror":"https://ror.org/03d0p2685","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I4210124929"]},{"id":"https://openalex.org/I4210125858","display_name":"Helmholtz Institute for RNA-based Infection Research","ror":"https://ror.org/02a98s891","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I4210124929","https://openalex.org/I4210125858"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Srinivas Manchalu","raw_affiliation_strings":["Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080 W\u00fcrzburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080 W\u00fcrzburg, Germany","institution_ids":["https://openalex.org/I4210124929","https://openalex.org/I4210125858"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014168314","display_name":"Benjamin N. Gray","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Benjamin N Gray","raw_affiliation_strings":["Benson Hill, St. Louis, MO, 63132, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Benson Hill, St. Louis, MO, 63132, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5053601159","display_name":"Chase L. Beisel","orcid":"https://orcid.org/0000-0003-0650-9943","observed_orcids":["https://orcid.org/0000-0003-0650-9943"]},"institutions":[{"id":"https://openalex.org/I137902535","display_name":"North Carolina State University","ror":"https://ror.org/04tj63d06","country_code":"US","type":"education","lineage":["https://openalex.org/I137902535"]},{"id":"https://openalex.org/I25974101","display_name":"University of W\u00fcrzburg","ror":"https://ror.org/00fbnyb24","country_code":"DE","type":"education","lineage":["https://openalex.org/I25974101"]},{"id":"https://openalex.org/I4210124929","display_name":"Helmholtz Centre for Infection Research","ror":"https://ror.org/03d0p2685","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I4210124929"]},{"id":"https://openalex.org/I4210125858","display_name":"Helmholtz Institute for RNA-based Infection Research","ror":"https://ror.org/02a98s891","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I4210124929","https://openalex.org/I4210125858"]}],"countries":["DE","US"],"is_corresponding":true,"raw_author_name":"Chase L Beisel","raw_affiliation_strings":["Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA","Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080 W\u00fcrzburg, Germany","Medical Faculty, University of W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"],"raw_orcid":"https://orcid.org/0000-0003-0650-9943","affiliations":[{"raw_affiliation_string":"Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA","institution_ids":["https://openalex.org/I137902535"]},{"raw_affiliation_string":"Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080 W\u00fcrzburg, Germany","institution_ids":["https://openalex.org/I4210124929","https://openalex.org/I4210125858"]},{"raw_affiliation_string":"Medical Faculty, University of W\u00fcrzburg, 97080 W\u00fcrzburg, Germany","institution_ids":["https://openalex.org/I25974101"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5053601159"],"corresponding_institution_ids":["https://openalex.org/I137902535","https://openalex.org/I25974101","https://openalex.org/I4210124929","https://openalex.org/I4210125858"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":{"value":3992,"currency":"USD","value_usd":3992},"fwci":2.5941,"has_fulltext":true,"cited_by_count":55,"citation_normalized_percentile":{"value":0.912395,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":98,"max":99},"biblio":{"volume":"48","issue":"10","first_page":"5624","last_page":"5638"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pam-recognition","display_name":"PAM recognition","score":0.8379999995231628},{"id":"https://openalex.org/keywords/prevotella-disiens","display_name":"Prevotella disiens","score":0.9950000047683716},{"id":"https://openalex.org/keywords/orthologs","display_name":"orthologs","score":0.5830000042915344},{"id":"https://openalex.org/keywords/guide-rna","display_name":"guide RNA","score":0.5600000023841858},{"id":"https://openalex.org/keywords/mutation-analysis","display_name":"mutation analysis","score":0.7139999866485596},{"id":"https://openalex.org/keywords/cell-free-transcription-translation","display_name":"cell-free transcription-translation","score":0.6539999842643738},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.5479999780654907}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9141479730606079},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8664570450782776},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.6400573253631592},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6128741502761841},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.6081968545913696},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5627796649932861},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.5436538457870483},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.5433490872383118},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4562077224254608},{"id":"https://openalex.org/C31909778","wikidata":"https://www.wikidata.org/wiki/Q7833921","display_name":"Transcription activator-like effector nuclease","level":5,"score":0.45556923747062683},{"id":"https://openalex.org/C35802184","wikidata":"https://www.wikidata.org/wiki/Q204773","display_name":"Zinc finger nuclease","level":5,"score":0.4501200318336487},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.44808709621429443},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.36939284205436707},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.26308584213256836},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.20236992835998535}],"mesh":[{"descriptor_ui":"D000072417","descriptor_name":"Protein Domains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004706","descriptor_name":"Endodeoxyribonucleases","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D004706","descriptor_name":"Endodeoxyribonucleases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004706","descriptor_name":"Endodeoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004706","descriptor_name":"Endodeoxyribonucleases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018720","descriptor_name":"Prevotella","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D053837","descriptor_name":"DNA Cleavage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064130","descriptor_name":"CRISPR-Associated Proteins","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D064130","descriptor_name":"CRISPR-Associated Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D064130","descriptor_name":"CRISPR-Associated Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D064130","descriptor_name":"CRISPR-Associated Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gkaa272","doi":"https://doi.org/10.1093/nar/gkaa272","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkaa272","pdf_url":"https://academic.oup.com/nar/article-pdf/48/10/5624/33326207/gkaa272.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7261169","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7261169","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:32329776","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32329776","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"},{"id":"pmh:oai:repository.helmholtz-hzi.de:10033/622825","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10033/622825","pdf_url":null,"source":{"id":"https://openalex.org/S4377196474","display_name":"HZI (Helmholtz Centre for Infection Research)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210124929","host_organization_name":"Helmholtz Centre for Infection Research","host_organization_lineage":["https://openalex.org/I4210124929"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"England","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1093/nar/gkaa272","doi":"https://doi.org/10.1093/nar/gkaa272","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkaa272","pdf_url":"https://academic.oup.com/nar/article-pdf/48/10/5624/33326207/gkaa272.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2137125854","display_name":"Synthetic Genetic Regulatory Networks to Study Emergent Properties of Development","funder_award_id":"1413044","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G8688801945","display_name":"Understanding and exploiting the functional diversity of CRISPR-Cas immune systems","funder_award_id":"R35GM119561","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3020041465.pdf","grobid_xml":"https://content.openalex.org/works/W3020041465.grobid-xml"},"referenced_works_count":50,"referenced_works":["https://openalex.org/W2021134519","https://openalex.org/W2043083543","https://openalex.org/W2059363769","https://openalex.org/W2062521268","https://openalex.org/W2106070783","https://openalex.org/W2107959600","https://openalex.org/W2122836421","https://openalex.org/W2131157113","https://openalex.org/W2135598273","https://openalex.org/W2149751339","https://openalex.org/W2165869860","https://openalex.org/W2169244146","https://openalex.org/W2327182906","https://openalex.org/W2337003117","https://openalex.org/W2339097657","https://openalex.org/W2341405505","https://openalex.org/W2410526873","https://openalex.org/W2465120080","https://openalex.org/W2515083702","https://openalex.org/W2557181747","https://openalex.org/W2558268315","https://openalex.org/W2613289296","https://openalex.org/W2621786780","https://openalex.org/W2623277795","https://openalex.org/W2741777336","https://openalex.org/W2742894922","https://openalex.org/W2765257165","https://openalex.org/W2785904295","https://openalex.org/W2787024379","https://openalex.org/W2793009196","https://openalex.org/W2793349369","https://openalex.org/W2793359586","https://openalex.org/W2803581084","https://openalex.org/W2890863912","https://openalex.org/W2891877869","https://openalex.org/W2896386549","https://openalex.org/W2898448050","https://openalex.org/W2902246058","https://openalex.org/W2915013877","https://openalex.org/W2919923263","https://openalex.org/W2921357732","https://openalex.org/W2927515220","https://openalex.org/W2940100772","https://openalex.org/W2949986034","https://openalex.org/W2952813308","https://openalex.org/W2952898285","https://openalex.org/W2954398758","https://openalex.org/W4206892629","https://openalex.org/W4239521868","https://openalex.org/W6748541284"],"related_works":["https://openalex.org/W4306974311","https://openalex.org/W4291142782","https://openalex.org/W4379137617","https://openalex.org/W2992626923","https://openalex.org/W4395010519","https://openalex.org/W4411383861","https://openalex.org/W4411886927","https://openalex.org/W4407591512","https://openalex.org/W2947146099","https://openalex.org/W4200248980"],"abstract_inverted_index":{"CRISPR-Cas":[0,28],"systems":[1,6,53],"comprise":[2],"diverse":[3],"adaptive":[4],"immune":[5],"in":[7,130,138,148,159],"prokaryotes":[8],"whose":[9],"RNA-directed":[10],"nucleases":[11,32,41,62,68,75,102,163],"have":[12,20],"been":[13],"co-opted":[14],"for":[15],"various":[16],"technologies.":[17],"Recent":[18],"efforts":[19],"focused":[21],"on":[22,96],"expanding":[23],"the":[24,37,64,185],"number":[25],"of":[26,40,184],"known":[27],"subtypes":[29],"to":[30,57,145,177],"identify":[31],"with":[33,122],"novel":[34],"properties.":[35],"However,":[36],"functional":[38],"diversity":[39],"within":[42],"each":[43,78],"subtype":[44],"remains":[45],"poorly":[46],"explored.":[47],"Here,":[48],"we":[49],"used":[50],"cell-free":[51],"transcription-translation":[52],"and":[54,87,108,136,181,191],"human":[55,160],"cells":[56],"characterize":[58],"six":[59],"Cas12a":[60,101,162],"single-effector":[61],"from":[63,152],"V-A":[65],"subtype,":[66],"including":[67],"sharing":[69],"high":[70],"sequence":[71],"identity.":[72],"While":[73],"these":[74],"readily":[76],"utilized":[77],"other's":[79],"guide":[80],"RNAs,":[81],"they":[82],"exhibited":[83],"distinct":[84,119,151],"PAM":[85,120,131,149,179,192],"profiles":[86,150],"apparent":[88],"targeting":[89],"activities":[90],"that":[91],"did":[92],"not":[93,125],"track":[94],"based":[95],"phylogeny.":[97],"In":[98],"particular,":[99],"two":[100],"encoded":[103],"by":[104],"Prevotella":[105,109],"ihumii":[106],"(PiCas12a)":[107],"disiens":[110],"(PdCas12a)":[111],"shared":[112],"over":[113],"95%":[114],"amino-acid":[115],"identity":[116],"yet":[117],"recognized":[118],"profiles,":[121],"PiCas12a":[123,144],"but":[124],"PdCas12a":[126,146],"accommodating":[127],"multiple":[128],"G's":[129],"positions":[132],"-2":[133],"through":[134,188],"-4":[135],"T":[137],"position":[139],"-1.":[140],"Mutational":[141],"analyses":[142],"transitioning":[143],"resulted":[147],"either":[153],"nuclease,":[154],"allowing":[155],"more":[156],"flexible":[157],"editing":[158],"cells.":[161],"therefore":[164],"can":[165],"exhibit":[166],"widely":[167],"varying":[168],"properties":[169],"between":[170],"otherwise":[171],"related":[172],"orthologs,":[173],"suggesting":[174],"selective":[175],"pressure":[176],"diversify":[178],"recognition":[180],"supporting":[182],"expansion":[183],"CRISPR":[186],"toolbox":[187],"ortholog":[189],"mining":[190],"engineering.":[193]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":11},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":11},{"year":2021,"cited_by_count":14}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2020-05-01T00:00:00"}
