{"id":"https://openalex.org/W3018874172","doi":"https://doi.org/10.1101/2020.04.21.053413","title":"Serpins: Purification and characterization of potent protease inhibitors from Clostridium thermocellum","display_name":"Serpins: Purification and characterization of potent protease inhibitors from Clostridium thermocellum","publication_year":2020,"publication_date":"2020-04-23","ids":{"openalex":"https://openalex.org/W3018874172","doi":"https://doi.org/10.1101/2020.04.21.053413","mag":"3018874172"},"language":"en","primary_location":{"id":"doi:10.1101/2020.04.21.053413","doi":"https://doi.org/10.1101/2020.04.21.053413","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2020.04.21.053413","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2020/04/23/2020.04.21.053413.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2020/04/23/2020.04.21.053413.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101731860","display_name":"Maria Mushtaq","orcid":"https://orcid.org/0000-0001-5307-6664","observed_orcids":["https://orcid.org/0000-0001-5307-6664"]},"institutions":[{"id":"https://openalex.org/I200749728","display_name":"Pir Mehr Ali Shah Arid Agriculture University","ror":"https://ror.org/035zn2q74","country_code":"PK","type":"education","lineage":["https://openalex.org/I200749728"]}],"countries":["PK"],"is_corresponding":true,"raw_author_name":"Maria Mushtaq","raw_affiliation_strings":["Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan"],"raw_orcid":"https://orcid.org/0000-0001-5307-6664","affiliations":[{"raw_affiliation_string":"Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan","institution_ids":["https://openalex.org/I200749728"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048041824","display_name":"Muhammad Javaid Asad","orcid":"https://orcid.org/0000-0001-8007-2431","observed_orcids":["https://orcid.org/0000-0001-8007-2431"]},"institutions":[{"id":"https://openalex.org/I200749728","display_name":"Pir Mehr Ali Shah Arid Agriculture University","ror":"https://ror.org/035zn2q74","country_code":"PK","type":"education","lineage":["https://openalex.org/I200749728"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Muhammad Javaid Asad","raw_affiliation_strings":["Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan","institution_ids":["https://openalex.org/I200749728"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002587072","display_name":"Muhammad Zeeshan Hyder","orcid":"https://orcid.org/0000-0002-9172-499X","observed_orcids":["https://orcid.org/0000-0002-9172-499X"]},"institutions":[{"id":"https://openalex.org/I16076960","display_name":"COMSATS University Islamabad","ror":"https://ror.org/00nqqvk19","country_code":"PK","type":"education","lineage":["https://openalex.org/I16076960"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Muhammad Zeeshan Hyder","raw_affiliation_strings":["Comsats Institute of Information and technology, Islamabad, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Comsats Institute of Information and technology, Islamabad, Pakistan","institution_ids":["https://openalex.org/I16076960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009977296","display_name":"Syed Muhammad Saqlan Naqvi","orcid":"https://orcid.org/0000-0002-5059-7461","observed_orcids":["https://orcid.org/0000-0002-5059-7461"]},"institutions":[{"id":"https://openalex.org/I1305695959","display_name":"Bacha Khan University","ror":"https://ror.org/02an6vg71","country_code":"PK","type":"education","lineage":["https://openalex.org/I1305695959"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Syed Muhammad Saqlan Naqvi","raw_affiliation_strings":["Bacha Khan University, Peshawar, KPK, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bacha Khan University, Peshawar, KPK, Pakistan","institution_ids":["https://openalex.org/I1305695959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086898769","display_name":"Saad Imran Malik","orcid":"https://orcid.org/0000-0002-0201-6896","observed_orcids":["https://orcid.org/0000-0002-0201-6896"]},"institutions":[{"id":"https://openalex.org/I200749728","display_name":"Pir Mehr Ali Shah Arid Agriculture University","ror":"https://ror.org/035zn2q74","country_code":"PK","type":"education","lineage":["https://openalex.org/I200749728"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Saad Imran Malik","raw_affiliation_strings":["Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan","institution_ids":["https://openalex.org/I200749728"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111088317","display_name":"Raja Tahir Mehmood","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I69716792","display_name":"Mirpur University of Science and Technology","ror":"https://ror.org/04qjkhc08","country_code":"PK","type":"education","lineage":["https://openalex.org/I69716792"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Raja Tahir Mehmood","raw_affiliation_strings":["MirPur University of Science and Technology, Azad Jammu and Kashmir"],"raw_orcid":"https://orcid.org/0000-0002-9242-2176","affiliations":[{"raw_affiliation_string":"MirPur University of Science and Technology, Azad Jammu and Kashmir","institution_ids":["https://openalex.org/I69716792"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5101731860"],"corresponding_institution_ids":["https://openalex.org/I200749728"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.486299991607666,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.486299991607666,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.3962000012397766,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.020600000396370888,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/serpins","display_name":"serpins","score":0.9750000238418579},{"id":"https://openalex.org/keywords/clostridium-thermocellum","display_name":"Clostridium thermocellum","score":0.9629999995231628},{"id":"https://openalex.org/keywords/serine-protease-inhibitors","display_name":"serine protease inhibitors","score":0.9409999847412109},{"id":"https://openalex.org/keywords/cellulosome","display_name":"cellulosome","score":0.9049999713897705},{"id":"https://openalex.org/keywords/protease-protection","display_name":"protease protection","score":0.5649999976158142},{"id":"https://openalex.org/keywords/trypsin","display_name":"trypsin","score":0.9240000247955322},{"id":"https://openalex.org/keywords/chymotrypsin","display_name":"chymotrypsin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/papain","display_name":"papain","score":0.9919999837875366},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.5440000295639038},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.5879999995231628},{"id":"https://openalex.org/keywords/ligation-independent-cloning","display_name":"ligation-independent cloning","score":0.9279999732971191}],"concepts":[{"id":"https://openalex.org/C2778965386","wikidata":"https://www.wikidata.org/wiki/Q420068","display_name":"Serpin","level":3,"score":0.7977923154830933},{"id":"https://openalex.org/C2779811187","wikidata":"https://www.wikidata.org/wiki/Q423655","display_name":"Subtilisin","level":3,"score":0.7390048503875732},{"id":"https://openalex.org/C2776061948","wikidata":"https://www.wikidata.org/wiki/Q3681172","display_name":"Clostridium thermocellum","level":4,"score":0.6654281616210938},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6114499568939209},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.5629108548164368},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5260270237922668},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.5184444785118103},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.32586362957954407},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2846066653728485},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1636313498020172},{"id":"https://openalex.org/C141603559","wikidata":"https://www.wikidata.org/wiki/Q410324","display_name":"Cellulase","level":3,"score":0.1345232129096985}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/2020.04.21.053413","doi":"https://doi.org/10.1101/2020.04.21.053413","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2020.04.21.053413","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2020/04/23/2020.04.21.053413.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1101/2020.04.21.053413","doi":"https://doi.org/10.1101/2020.04.21.053413","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2020.04.21.053413","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2020/04/23/2020.04.21.053413.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310246","display_name":"University of Rochester","ror":"https://ror.org/022kthw22"},{"id":"https://openalex.org/F4320326275","display_name":"Higher Education Commision, Pakistan","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3018874172.pdf","grobid_xml":"https://content.openalex.org/works/W3018874172.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W1522565043","https://openalex.org/W1998498139","https://openalex.org/W2048543680","https://openalex.org/W2052186537","https://openalex.org/W2052674778","https://openalex.org/W2053786654","https://openalex.org/W2097817417","https://openalex.org/W2100837269","https://openalex.org/W2182303898","https://openalex.org/W2322015944","https://openalex.org/W2562647913","https://openalex.org/W2621537980","https://openalex.org/W2794623172","https://openalex.org/W2913716804","https://openalex.org/W2936430728","https://openalex.org/W4238052567"],"related_works":["https://openalex.org/W1974040671","https://openalex.org/W2116269039","https://openalex.org/W2571436447","https://openalex.org/W2183609357","https://openalex.org/W2019716166","https://openalex.org/W1979625372","https://openalex.org/W2024709755","https://openalex.org/W1628885686","https://openalex.org/W4361284289","https://openalex.org/W2171689630"],"abstract_inverted_index":{"Abstract":[0],"Clostridium":[1],"thermocellum":[2,54],"produces":[3],"an":[4],"extracellular":[5,18],"cellulosome":[6],"(a":[7],"multiprotein":[8],"complex":[9],"produced":[10],"by":[11],"firmicutes":[12],"bacteria),":[13],"which,":[14],"owing":[15],"to":[16,22],"its":[17],"location,":[19],"is":[20],"open":[21],"protease":[23,26,32],"attack.":[24,33],"Serine":[25],"inhibitors":[27],"(serpins)":[28],"protect":[29],"bacteria":[30],"against":[31,99,105],"However,":[34],"their":[35],"structure":[36],"and":[37,45,68,72,82,84,102,108],"function":[38],"are":[39],"poorly":[40],"characterized.":[41],"This":[42],"study":[43],"identified":[44],"amplified":[46],"the":[47,52,61,65],"serpin":[48,94],"1270":[49,95],"gene":[50],"from":[51],"C.":[53],"genome.":[55],"Purified":[56],"serpins":[57],"were":[58,88],"cloned":[59],"into":[60,74],"pTXB1":[62],"vector":[63],"using":[64],"one-step":[66],"sequence":[67],"ligation-independent":[69],"cloning":[70],"reaction":[71],"transformed":[73],"Escherichia":[75],"coli":[76],"BL21":[77],"DE3":[78],"cells.":[79],"Enzyme":[80],"overexpression":[81],"purification":[83],"enzyme":[85],"inhibitory":[86],"assays":[87],"performed.":[89],"The":[90],"results":[91],"showed":[92],"that":[93],"has":[96],"89%":[97],"inhibition":[98,104],"Bacillus":[100],"subtilisin":[101],"64%":[103],"trypsin,":[106],"chymotrypsin,":[107],"papain.":[109]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2020-05-01T00:00:00"}
