{"id":"https://openalex.org/W3017127982","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.00569","title":"A High Throughput Approach to Biochemically Characterizing Firefly Luciferase for Use in Multi\u2010Component Bioluminescent Imaging","display_name":"A High Throughput Approach to Biochemically Characterizing Firefly Luciferase for Use in Multi\u2010Component Bioluminescent Imaging","publication_year":2020,"publication_date":"2020-04-01","ids":{"openalex":"https://openalex.org/W3017127982","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.00569","mag":"3017127982"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.2020.34.s1.00569","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.00569","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.2020.34.s1.00569","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5069843781","display_name":"Edison S. Reid\u2010McLaughlin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210161782","display_name":"W.M. Keck Science Center","ror":"https://ror.org/05sy8gb82","country_code":"US","type":"facility","lineage":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960","https://openalex.org/I4210161782"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edison Esme Reid-McLaughlin","raw_affiliation_strings":["W.M. Keck Science Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W.M. Keck Science Center","institution_ids":["https://openalex.org/I4210161782"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027262066","display_name":"Lyndsey Flanagan","orcid":"https://orcid.org/0000-0002-5110-960X","observed_orcids":["https://orcid.org/0000-0002-5110-960X"]},"institutions":[{"id":"https://openalex.org/I4210161782","display_name":"W.M. Keck Science Center","ror":"https://ror.org/05sy8gb82","country_code":"US","type":"facility","lineage":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960","https://openalex.org/I4210161782"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lyndsey Flanagan","raw_affiliation_strings":["W.M. Keck Science Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W.M. Keck Science Center","institution_ids":["https://openalex.org/I4210161782"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047874372","display_name":"Aaron M. Leconte","orcid":"https://orcid.org/0000-0002-5789-9147","observed_orcids":["https://orcid.org/0000-0002-5789-9147"]},"institutions":[{"id":"https://openalex.org/I4210161782","display_name":"W.M. Keck Science Center","ror":"https://ror.org/05sy8gb82","country_code":"US","type":"facility","lineage":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960","https://openalex.org/I4210161782"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aaron Leconte","raw_affiliation_strings":["W.M. Keck Science Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W.M. Keck Science Center","institution_ids":["https://openalex.org/I4210161782"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210161782"],"apc_list":{"value":2500,"currency":"USD","value_usd":2500},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"34","issue":"S1","first_page":"1","last_page":"1"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10097","display_name":"Luminescence Properties of Advanced Materials","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/firefly-luciferase","display_name":"firefly luciferase","score":1.0},{"id":"https://openalex.org/keywords/bioluminescence-imaging","display_name":"bioluminescence imaging","score":0.9070000052452087},{"id":"https://openalex.org/keywords/substrate-recognition","display_name":"substrate recognition","score":0.609000027179718},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7059999704360962},{"id":"https://openalex.org/keywords/protein-engineering","display_name":"protein engineering","score":0.621999979019165},{"id":"https://openalex.org/keywords/in-vivo-imaging","display_name":"in vivo imaging","score":0.503000020980835}],"concepts":[{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.8032451868057251},{"id":"https://openalex.org/C5176359","wikidata":"https://www.wikidata.org/wiki/Q179924","display_name":"Bioluminescence","level":2,"score":0.7852839231491089},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6508362293243408},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.5758326053619385},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.49322909116744995},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.4832056760787964},{"id":"https://openalex.org/C125245961","wikidata":"https://www.wikidata.org/wiki/Q221656","display_name":"Brightness","level":2,"score":0.48081648349761963},{"id":"https://openalex.org/C2909536089","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferases","level":5,"score":0.47741973400115967},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.4565427601337433},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.45070314407348633},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.44644638895988464},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.42091453075408936},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4079650640487671},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3594561815261841},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31622615456581116},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.24925589561462402},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11851784586906433},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.10537078976631165},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.0902528464794159},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.2020.34.s1.00569","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.00569","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.2020.34.s1.00569","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7035974322","https://openalex.org/W2791958166","https://openalex.org/W2588587216","https://openalex.org/W7056371962","https://openalex.org/W3046788251","https://openalex.org/W7030653033","https://openalex.org/W7026583082","https://openalex.org/W4253858024","https://openalex.org/W1987229538","https://openalex.org/W6999682285"],"abstract_inverted_index":{"Bioluminescent":[0],"imaging":[1,10],"(BLI)":[2],"is":[3,46,54,59,263],"a":[4,55,63,87,103,182,203,227,233,244,282,309],"foundational":[5],"tool":[6],"for":[7,96,144,202,336,346],"in":[8,28,102,156,168,177,179,196,224,236,243,265,275],"vivo":[9],"routinely":[11],"used":[12,48,305],"to":[13,31,49,68,74,90,116,220,306],"accurately":[14],"visualize":[15],"cells":[16],"within":[17],"mouse":[18],"bodies":[19],"and":[20,58,94,135,181,189,216,271,321],"brains.":[21],"However,":[22],"these":[23,51,255],"probes":[24],"are":[25],"currently":[26],"limited":[27,61],"their":[29,41,145],"ability":[30],"image":[32],"multiple":[33],"targets":[34],"at":[35,129,151,172,191,286,312],"once":[36],"as":[37,39,137],"well":[38],"by":[40,62,113,232,342],"brightness.":[42,253],"While":[43],"directed":[44],"evolution":[45],"commonly":[47],"improve":[50],"enzymes,":[52],"it":[53],"slow":[56],"approach":[57,107,262,302],"often":[60],"lack":[64],"of":[65,159,198,205,269,284,288,327],"biochemical":[66,78,325],"knowledge":[67],"guide":[69],"amino":[70,99,131,314],"acid":[71,100,132,315],"changes.":[72],"Here,":[73],"rapidly":[75,117,307],"generate":[76],"high\u2010value":[77],"information":[79],"on":[80,147],"the":[81,152,160,173,192,199,239,248,289,343],"enzyme":[82],"luciferase,":[83],"we":[84,125,279],"have":[85,221,280,295],"developed":[86],"high\u2010throughput":[88,261],"platform":[89],"categorize":[91],"substrate":[92,230,250],"recognition":[93],"brightness":[95,225,237,297],"many":[97],"possible":[98],"changes":[101],"single":[104],"experiment.":[105],"Our":[106],"couples":[108],"screening":[109],"small":[110,234],"libraries":[111,268],"followed":[112],"next\u2010generation":[114],"sequencing":[115],"create":[118],"sequence\u2010function":[119,310],"maps.":[120],"As":[121],"an":[122,222],"initial":[123],"foray,":[124],"screened":[126],"all":[127],"mutations":[128,150,171,190],"three":[130,290],"positions\u2014A482,":[133],"N229,":[134],"G246;":[136],"expected,":[138],"each":[139],"position":[140,154,175,194],"showed":[141,208],"unique":[142],"patterns":[143],"impact":[146],"function.":[148,276],"Generally,":[149],"482":[153],"resulted":[155,176,195],"either":[157],"inactivation":[158,197],"protein":[161,200,319],"or":[162,165,332],"no":[163],"change":[164],"slight":[166],"decrease":[167,178,235],"function,":[169],"while":[170],"229":[174],"function":[180],"large":[183],"shift":[184],"towards":[185,247],"red":[186],"light":[187],"emission,":[188],"246":[193],"except":[201],"handful":[204,283],"mutants":[206,270,285],"that":[207,258,294],"increased":[209],"functionality.":[210],"In":[211,277],"addition,":[212,278],"two":[213,287],"mutants,":[214],"N229G":[215],"G246S,":[217],"were":[218],"observed":[219],"increase":[223],"with":[226,238,298],"modified":[228],"luciferin":[229],"accompanied":[231],"natural":[240],"substrate,":[241],"resulting":[242],"specificity":[245],"shifted":[246],"analogous":[249],"without":[251],"compromised":[252],"Collectively,":[254],"data":[256],"illustrate":[257],"our":[259,323],"novel,":[260],"reliable":[264],"creating":[266],"diverse":[267],"detecting":[272],"true":[273],"enrichment":[274],"identified":[281],"residues":[291],"investigate":[292],"here":[293],"improved":[296],"non\u2010native":[299],"substrates.":[300],"This":[301],"can":[303],"be":[304],"build":[308],"map":[311],"any":[313],"position,":[316],"facilitating":[317],"future":[318],"engineering":[320],"improving":[322],"basic":[324],"understanding":[326],"this":[328,337],"important":[329],"enzyme.":[330],"Support":[331],"Funding":[333,335],"Information":[334],"research":[338],"has":[339],"been":[340],"provided":[341],"Research":[344],"Corporation":[345],"Science":[347],"Advancement.":[348]},"counts_by_year":[],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2020-04-24T00:00:00"}