{"id":"https://openalex.org/W3016861478","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.06975","title":"MCF7 Human Breast Cancer Cells Engineered to Stably Overexpress DEPTOR via CRISPR/Cas9 to Reduce Anabolic Capacity","display_name":"MCF7 Human Breast Cancer Cells Engineered to Stably Overexpress DEPTOR via CRISPR/Cas9 to Reduce Anabolic Capacity","publication_year":2020,"publication_date":"2020-04-01","ids":{"openalex":"https://openalex.org/W3016861478","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.06975","mag":"3016861478"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.2020.34.s1.06975","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.06975","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.2020.34.s1.06975","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014288188","display_name":"John William Deaver","orcid":"https://orcid.org/0000-0003-1280-8499","observed_orcids":["https://orcid.org/0000-0003-1280-8499"]},"institutions":[{"id":"https://openalex.org/I91045830","display_name":"Texas A&M University","ror":"https://ror.org/01f5ytq51","country_code":"US","type":"education","lineage":["https://openalex.org/I91045830"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John William Deaver","raw_affiliation_strings":["Texas A&amp;M University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Texas A&amp;M University","institution_ids":["https://openalex.org/I91045830"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077334654","display_name":"Sara Mata L\u00f3pez","orcid":"https://orcid.org/0000-0002-0280-7217","observed_orcids":["https://orcid.org/0000-0002-0280-7217"]},"institutions":[{"id":"https://openalex.org/I91045830","display_name":"Texas A&M University","ror":"https://ror.org/01f5ytq51","country_code":"US","type":"education","lineage":["https://openalex.org/I91045830"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sara Mata L\u00f3pez","raw_affiliation_strings":["Texas A&amp;M University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Texas A&amp;M University","institution_ids":["https://openalex.org/I91045830"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071737472","display_name":"Peter P. Nghiem","orcid":"https://orcid.org/0000-0002-8796-8123","observed_orcids":["https://orcid.org/0000-0002-8796-8123"]},"institutions":[{"id":"https://openalex.org/I91045830","display_name":"Texas A&M University","ror":"https://ror.org/01f5ytq51","country_code":"US","type":"education","lineage":["https://openalex.org/I91045830"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter P. Nghiem","raw_affiliation_strings":["Texas A&amp;M University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Texas A&amp;M University","institution_ids":["https://openalex.org/I91045830"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059467218","display_name":"Melinda Sheffield\u2010Moore","orcid":"https://orcid.org/0000-0001-6579-4655","observed_orcids":["https://orcid.org/0000-0001-6579-4655"]},"institutions":[{"id":"https://openalex.org/I91045830","display_name":"Texas A&M University","ror":"https://ror.org/01f5ytq51","country_code":"US","type":"education","lineage":["https://openalex.org/I91045830"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melinda Sheffield-Moore","raw_affiliation_strings":["Texas A&amp;M University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Texas A&amp;M University","institution_ids":["https://openalex.org/I91045830"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001798388","display_name":"Steven E. Riechman","orcid":"https://orcid.org/0000-0001-9439-7560","observed_orcids":["https://orcid.org/0000-0001-9439-7560"]},"institutions":[{"id":"https://openalex.org/I91045830","display_name":"Texas A&M University","ror":"https://ror.org/01f5ytq51","country_code":"US","type":"education","lineage":["https://openalex.org/I91045830"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven E. Riechman","raw_affiliation_strings":["Texas A&amp;M University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Texas A&amp;M University","institution_ids":["https://openalex.org/I91045830"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026323332","display_name":"James D. Fluckey","orcid":"https://orcid.org/0000-0003-1231-0412","observed_orcids":["https://orcid.org/0000-0003-1231-0412"]},"institutions":[{"id":"https://openalex.org/I91045830","display_name":"Texas A&M University","ror":"https://ror.org/01f5ytq51","country_code":"US","type":"education","lineage":["https://openalex.org/I91045830"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James D. Fluckey","raw_affiliation_strings":["Texas A&amp;M University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Texas A&amp;M University","institution_ids":["https://openalex.org/I91045830"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I91045830"],"apc_list":{"value":2500,"currency":"USD","value_usd":2500},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"34","issue":"S1","first_page":"1","last_page":"1"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.007000000216066837,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11399","display_name":"Muscle metabolism and nutrition","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/deptor","display_name":"DEPTOR","score":0.9860000014305115},{"id":"https://openalex.org/keywords/mtor-inhibition","display_name":"mTOR inhibition","score":0.7459999918937683},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"breast cancer","score":0.8510000109672546},{"id":"https://openalex.org/keywords/mcf-7-cells","display_name":"MCF-7 cells","score":0.9909999966621399},{"id":"https://openalex.org/keywords/crispr-cas9-genome-editing","display_name":"CRISPR/Cas9 genome editing","score":0.8130000233650208},{"id":"https://openalex.org/keywords/aavs1-safe-harbor","display_name":"AAVS1 safe harbor","score":0.847000002861023},{"id":"https://openalex.org/keywords/protein-synthesis","display_name":"protein synthesis","score":0.765999972820282},{"id":"https://openalex.org/keywords/anabolic-metabolism","display_name":"anabolic metabolism","score":0.6549999713897705},{"id":"https://openalex.org/keywords/mtor-signaling","display_name":"mTOR signaling","score":0.5059999823570251},{"id":"https://openalex.org/keywords/stable-isotope-labeling","display_name":"stable isotope labeling","score":0.48399999737739563},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.3109999895095825},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.3529999852180481},{"id":"https://openalex.org/keywords/cell-migration","display_name":"cell migration","score":0.3089999854564667},{"id":"https://openalex.org/keywords/cell-invasion","display_name":"cell invasion","score":0.531000018119812}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.6132772564888},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.518284022808075},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.506508469581604},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4719661474227905},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.4527833163738251},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4351869225502014},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3841294050216675},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.3267141580581665},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.28761380910873413},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19388636946678162},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.16494882106781006}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.2020.34.s1.06975","doi":"https://doi.org/10.1096/fasebj.2020.34.s1.06975","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.2020.34.s1.06975","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"display_name":"Clean water and sanitation","score":0.4399999976158142,"id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4407518363","https://openalex.org/W3165403377","https://openalex.org/W3174465856","https://openalex.org/W1605833010","https://openalex.org/W4409691441","https://openalex.org/W3174588855","https://openalex.org/W2572265768","https://openalex.org/W4402491857","https://openalex.org/W1974081053","https://openalex.org/W1997177694"],"abstract_inverted_index":{"As":[0],"a":[1,42,57,77,95,99,147,153,161,184,190,193,208,283],"potent":[2],"cellular":[3,34,47,119,251],"inhibitor":[4],"of":[5,20,28,45,59,80,98,118,125,130,133,146,179,201,221,228,238,261,350,425,443,461,464,475,504],"both":[6,370],"mTOR":[7],"complexes,":[8],"DEPTOR":[9,29,102,139,150,203,286,418,467],"protein":[10,30,196,214,239,262,287,436],"has":[11],"the":[12,17,81,105,123,127,167,202,296,397,404,422,444,459,471,495,502],"potential":[13,19,129],"to":[14,68,93,121,225,270,291,300,338,347,406],"dramatically":[15,420],"alter":[16],"anabolic":[18,128,252,423],"many":[21],"different":[22,340,411],"cell":[23,88],"types":[24],"and":[25,115,217,231,250,321,343,381,454,484,501,506],"conditions.":[26],"Manipulation":[27],"content":[31,288],"means":[32],"that":[33,417],"anabolism":[35,120],"can":[36],"be":[37,53,292,339,458],"more":[38],"aggressively":[39],"regulated":[40],"at":[41,166],"single":[43],"bottleneck":[44],"key":[46],"signaling":[48,253],"pathways.":[49],"This":[50],"approach":[51],"could":[52,457],"particularly":[54],"advantageous":[55],"in":[56,199,267,295,356,396,435,452,479],"number":[58],"disease":[60],"states":[61],"where":[62],"anabolically":[63],"aggressive":[64],"behavior":[65],"gives":[66],"rise":[67],"specific":[69],"pathology.":[70],"To":[71],"evaluate":[72],"this":[73,131],"concept,":[74],"we":[75,112],"developed":[76],"novel":[78],"subset":[79],"immortalized":[82],"MCF7":[83,136,426],"human":[84,106,134,168,427],"epithelial":[85],"breast":[86],"cancer":[87,428],"line.":[89],"By":[90],"utilizing":[91],"CRISPR/Cas9":[92],"facilitate":[94],"targeted":[96],"insertion":[97],"constitutively":[100,148],"active":[101,149],"gene":[103,151,198],"into":[104],"AAVS1":[107,169],"genomic":[108],"safe":[109,170],"harbor":[110,171],"site,":[111],"examined":[113],"direct":[114],"indirect":[116],"measures":[117],"determine":[122],"efficacy":[124],"abating":[126],"type":[132],"cancer.":[135],"cells":[137,180,222],"overexpressing":[138],"(DEP)":[140],"were":[141,181,223,241,264,335,393,409],"generated":[142],"by":[143,173,186,432,466,494],"genome":[144],"integration":[145],"via":[152,256],"homologous":[154],"directed":[155],"repair":[156],"(HDR)":[157],"based":[158],"template":[159],"following":[160],"double":[162],"stranded":[163],"break":[164],"(DSB)":[165],"site":[172],"Cas9":[174],"nuclease.":[175],"A":[176],"second":[177],"group":[178,298],"used":[182],"as":[183,430,439,441],"control":[185],"treating":[187],"them":[188],"with":[189],"vector":[191],"containing":[192],"red":[194],"fluorescent":[195],"(RFP)":[197],"lieu":[200],"gene.":[204],"Both":[205,219],"vectors":[206],"contain":[207],"separate":[209],"promoter":[210],"for":[211,473],"green":[212],"fluorescence":[213],"(GFP)":[215],"gene,":[216],"N\u2010acetyltransferase.":[218],"groups":[220],"subjected":[224],"14":[226],"days":[227],"puromycin":[229],"selection,":[230],"appropriate":[232],"fluorophore":[233],"presence":[234],"was":[235,254,289,354],"confirmed.":[236],"Rates":[237],"synthesis":[240,263,437],"measured":[242],"using":[243],"deuterium":[244],"heavy":[245],"water":[246],"stable":[247],"isotope":[248],"methods,":[249],"assessed":[255],"immunoblotting.":[257],"Twenty\u2010four":[258],"hour":[259],"rates":[260],"significantly":[265,293,394],"reduced":[266],"DEP":[268,297,342,357,398],"compared":[269,299],"RFP":[271,301,359,400],"(23.390":[272],"\u00b1":[273,277,304,308,314,318,324,328,361,365,374,378,386,390],"2.26":[274],"vs.":[275,306],"37.314":[276],"0.21":[278],"%/day,":[279],"respectively;":[280,310,367],"p=0.029),":[281],"representing":[282],"37.6%":[284],"reduction.":[285],"found":[290,337],"higher":[294,395],"controls":[302],"(9.593":[303],"3.209":[305],"1":[307,317,327,377,389],"0.552,":[309],"p<0.001).":[311],"Total":[312],"(0.978":[313],"0.202":[315],"vs":[316,326,358,363,376,388,399],"0.162;":[319],"p=0.841)":[320],"phosphorylated":[322,346,405,453],"(0.65":[323],"0.445":[325],"0.406;":[329],"p=0.186)":[330],"p70":[331,351,448],"S6":[332,352,449],"Kinase":[333,353],"Thr389":[334],"not":[336,410],"between":[341,412,482],"RFP.":[344],"The":[345],"total":[348,371,407,455],"ratio":[349],"lower":[355],"(0.619":[360],"0.315":[362],"0.978":[364],"0.255,":[366],"p=0.05).":[368],"Interestingly,":[369],"4E\u2010BP1":[372,456],"(1.340":[373],"0.107":[375],"0.102,":[379],"p<0.001)":[380],"Thr":[382],"37/46":[383],"phosphorylation":[384,442],"(2.106":[385],"0.742":[387],"0.559,":[391],"p=0.015)":[392],"groups,":[401],"respectively,":[402],"but":[403],"ratios":[408],"groups.":[413],"These":[414],"results":[415],"indicate":[416],"overexpression":[419],"impacts":[421],"activity":[424],"cells,":[429],"shown":[431],"significant":[433],"reductions":[434],"rates,":[438],"well":[440],"downstream":[445],"mTORC1":[446,465],"target":[447],"Kinase.":[450],"Increases":[451],"result":[460],"long\u2010term":[462],"inhibition":[463],"protein,":[468],"possibly":[469],"indicating":[470],"need":[472],"evaluation":[474],"4E\u2010BP1\u2019s":[476],"unique":[477],"role":[478],"regulating":[480],"transitions":[481],"Cap\u2010Dependent":[483],"Cap\u2010Independent":[485],"mRNA":[486],"translation.":[487],"Support":[488],"or":[489],"Funding":[490,492],"Information":[491],"provided":[493],"Sydney":[496],"&":[497],"JL":[498],"Huffine\u2019s":[499],"Institute":[500],"College":[503],"Education":[505],"Human":[507],"Development.":[508]},"counts_by_year":[],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2020-04-24T00:00:00"}