{"id":"https://openalex.org/W3013065798","doi":null,"title":"Mutational Analysis of the SPOTS Complex Component ORM1","display_name":"Mutational Analysis of the SPOTS Complex Component ORM1","publication_year":2019,"publication_date":"2019-11-01","ids":{"openalex":"https://openalex.org/W3013065798","mag":"3013065798"},"language":"en","primary_location":{"id":"pmh:oai:utoronto.scholaris.ca:1807/98159","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/98159","pdf_url":"https://utoronto.scholaris.ca/bitstreams/91b44c68-8213-402f-90b1-91d297cc8db4/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://utoronto.scholaris.ca/bitstreams/91b44c68-8213-402f-90b1-91d297cc8db4/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087450312","display_name":"Omar Mourad","orcid":"https://orcid.org/0000-0003-1103-6781","observed_orcids":["https://orcid.org/0000-0003-1103-6781"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Omar Mourad","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1103-6781","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5087450312"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11308","display_name":"Sphingolipid Metabolism and Signaling","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11308","display_name":"Sphingolipid Metabolism and Signaling","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12465","display_name":"Cancer, Lipids, and Metabolism","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/orm1","display_name":"ORM1","score":0.9570000171661377},{"id":"https://openalex.org/keywords/serine-palmitoyltransferase","display_name":"serine palmitoyltransferase","score":0.9100000262260437},{"id":"https://openalex.org/keywords/de-novo-sphingolipid-biosynthesis","display_name":"de novo sphingolipid biosynthesis","score":0.9229999780654907},{"id":"https://openalex.org/keywords/protein-structure","display_name":"protein structure","score":0.4580000042915344},{"id":"https://openalex.org/keywords/endoplasmic-reticulum-membrane-protein","display_name":"endoplasmic reticulum membrane protein","score":0.7990000247955322}],"concepts":[{"id":"https://openalex.org/C168167062","wikidata":"https://www.wikidata.org/wiki/Q1117970","display_name":"Component (thermodynamics)","level":2,"score":0.5693773627281189},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.19771724939346313},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:utoronto.scholaris.ca:1807/98159","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/98159","pdf_url":"https://utoronto.scholaris.ca/bitstreams/91b44c68-8213-402f-90b1-91d297cc8db4/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:3013065798","doi":null,"is_oa":false,"landing_page_url":"https://tspace.library.utoronto.ca/bitstream/1807/98159/1/Mourad_Omar_201911_MSc_thesis.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:utoronto.scholaris.ca:1807/98159","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/98159","pdf_url":"https://utoronto.scholaris.ca/bitstreams/91b44c68-8213-402f-90b1-91d297cc8db4/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3013065798.pdf","grobid_xml":"https://content.openalex.org/works/W3013065798.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W193657971","https://openalex.org/W1256777839","https://openalex.org/W1770154540","https://openalex.org/W1830235003","https://openalex.org/W1963731318","https://openalex.org/W1991655565","https://openalex.org/W2008599922","https://openalex.org/W2026069540","https://openalex.org/W2030035274","https://openalex.org/W2032977708","https://openalex.org/W2038379107","https://openalex.org/W2049260737","https://openalex.org/W2049619195","https://openalex.org/W2058006650","https://openalex.org/W2063968358","https://openalex.org/W2064066807","https://openalex.org/W2068989744","https://openalex.org/W2078040293","https://openalex.org/W2079559417","https://openalex.org/W2099944258","https://openalex.org/W2111724662","https://openalex.org/W2114746301","https://openalex.org/W2114881940","https://openalex.org/W2125872855","https://openalex.org/W2126835609","https://openalex.org/W2126953096","https://openalex.org/W2132926880","https://openalex.org/W2137058218","https://openalex.org/W2147655543","https://openalex.org/W2153820291","https://openalex.org/W2163317648","https://openalex.org/W2171878310","https://openalex.org/W2418806051","https://openalex.org/W2520513997","https://openalex.org/W2597928593","https://openalex.org/W2601232135","https://openalex.org/W2750788866","https://openalex.org/W2783587949","https://openalex.org/W2786841771","https://openalex.org/W2889258318","https://openalex.org/W2904355820","https://openalex.org/W2912324706"],"related_works":["https://openalex.org/W4401794228","https://openalex.org/W2912324706","https://openalex.org/W7095076964","https://openalex.org/W2171878310","https://openalex.org/W3173287334","https://openalex.org/W4403908946","https://openalex.org/W4391286654","https://openalex.org/W2909939805","https://openalex.org/W2052601330","https://openalex.org/W2012369821"],"abstract_inverted_index":{"The":[0,59],"Orm":[1,19,45,88],"family":[2],"of":[3,14,38,87,94,107],"proteins":[4,20,26,89],"are":[5,21],"conserved":[6,102],"throughout":[7],"eukaryotes":[8],"and":[9,49,57,68],"serve":[10],"as":[11],"negative":[12],"regulators":[13],"de":[15],"novo":[16],"sphingolipid":[17,39],"biosynthesis.":[18,40],"integral":[22],"endoplasmic":[23],"reticulum":[24],"membrane":[25],"that":[27,141],"associate":[28],"with":[29,65,136],"serine":[30,137],"palmitoyltransferase,":[31],"which":[32],"catalyzes":[33],"the":[34,71,85,95,100,108],"first":[35],"committed":[36],"step":[37],"Mammalian":[41],"genomes":[42],"encode":[43],"three":[44],"paralogs,":[46,55],"ORMDL1,":[47],"ORMDL2":[48],"ORMDL3,":[50],"while":[51],"yeast":[52,96],"express":[53],"two":[54],"ORM1":[56],"ORM2.":[58],"ORMs":[60],"contain":[61],"four":[62],"transmembrane":[63],"domains":[64],"both":[66],"N":[67],"C-termini":[69],"in":[70,113,117,126],"cytosol":[72],"but":[73],"beyond":[74],"this":[75,142],"little":[76],"is":[77,120],"known":[78],"about":[79],"their":[80],"structure.":[81],"To":[82],"better":[83],"understand":[84],"function":[86],"I":[90,139],"performed":[91],"targeted":[92],"mutagenesis":[93],"ORM1,":[97],"focusing":[98],"on":[99],"highly":[101],"cytosolic":[103],"loop.":[104],"Specifically,":[105],"deletion":[106],"invariant":[109],"WEQ148-150":[110],"residues":[111],"results":[112],"an":[114],"apparent":[115],"reduction":[116],"function.":[118],"This":[119],"not":[121],"attributable":[122],"to":[123,131,134],"a":[124],"defect":[125],"Orm1\u0394WEQ":[127],"protein":[128],"localization,":[129],"nor":[130],"its":[132],"ability":[133],"interact":[135],"palmitoyltransferase.":[138],"hypothesize":[140],"region":[143],"may":[144],"be":[145],"critical":[146],"for":[147],"detecting":[148],"ceramide.":[149]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2020-04-03T00:00:00"}