{"id":"https://openalex.org/W3010173556","doi":null,"title":"Development of an aptamer based lateral flow test strip for a diabetes biomarker","display_name":"Development of an aptamer based lateral flow test strip for a diabetes biomarker","publication_year":2019,"publication_date":"2019-01-01","ids":{"openalex":"https://openalex.org/W3010173556","mag":"3010173556"},"language":"en","primary_location":{"id":"pmh:oai:etd.uwc.ac.za:11394/7156","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11394/7156","pdf_url":"http://hdl.handle.net/11394/7156","source":{"id":"https://openalex.org/S4306401169","display_name":"University of the Western Cape Electronic Theses and Dissertations Repository (University of the Western Cape)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I869660684","host_organization_name":"University of the Western Cape","host_organization_lineage":["https://openalex.org/I869660684"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/11394/7156","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081420814","display_name":"Koena Leah Moabelo","orcid":"https://orcid.org/0000-0002-9114-4530","observed_orcids":["https://orcid.org/0000-0002-9114-4530"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Koena Leah Moabelo","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9114-4530","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5081420814"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.021299999207258224,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aptamers","display_name":"aptamers","score":0.8939999938011169},{"id":"https://openalex.org/keywords/lateral-flow-test-strip","display_name":"lateral flow strip","score":0.8930000066757202},{"id":"https://openalex.org/keywords/diabetes-mellitus","display_name":"diabetes mellitus","score":0.6859999895095825},{"id":"https://openalex.org/keywords/retinol-binding-protein-4","display_name":"retinol-binding protein 4","score":0.9760000109672546},{"id":"https://openalex.org/keywords/point-of-care-testing","display_name":"point-of-care testing","score":0.8700000047683716},{"id":"https://openalex.org/keywords/colorimetric-aptasensor","display_name":"colorimetric aptasensor","score":0.9549999833106995},{"id":"https://openalex.org/keywords/gold-nanoparticles","display_name":"gold nanoparticles","score":0.8069999814033508},{"id":"https://openalex.org/keywords/sandwich-elisa","display_name":"sandwich ELISA","score":0.5569999814033508},{"id":"https://openalex.org/keywords/aptamer-binding-affinity","display_name":"aptamer binding affinity","score":0.6290000081062317},{"id":"https://openalex.org/keywords/in-silico-aptamer-design","display_name":"in silico aptamer design","score":0.6669999957084656},{"id":"https://openalex.org/keywords/low-cost-diagnostic-kit","display_name":"low-cost diagnostic kit","score":0.4399999976158142},{"id":"https://openalex.org/keywords/aptamer-secondary-structure","display_name":"aptamer secondary structure","score":0.3269999921321869}],"concepts":[{"id":"https://openalex.org/C128972844","wikidata":"https://www.wikidata.org/wiki/Q418722","display_name":"Aptamer","level":2,"score":0.9877243041992188},{"id":"https://openalex.org/C106847996","wikidata":"https://www.wikidata.org/wiki/Q898756","display_name":"Surface plasmon resonance","level":3,"score":0.5798207521438599},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5004141330718994},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.48967769742012024},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3191605806350708},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28007957339286804},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2700854539871216},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.26174572110176086},{"id":"https://openalex.org/C155672457","wikidata":"https://www.wikidata.org/wiki/Q61231","display_name":"Nanoparticle","level":2,"score":0.2090195119380951},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.19933676719665527},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08508706092834473}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:etd.uwc.ac.za:11394/7156","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11394/7156","pdf_url":"http://hdl.handle.net/11394/7156","source":{"id":"https://openalex.org/S4306401169","display_name":"University of the Western Cape Electronic Theses and Dissertations Repository (University of the Western Cape)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I869660684","host_organization_name":"University of the Western Cape","host_organization_lineage":["https://openalex.org/I869660684"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":null},{"id":"pmh:oai:uwcscholar.uwc.ac.za:10566/13374","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/10566/13374","pdf_url":"https://uwcscholar.uwc.ac.za/bitstreams/536967af-8014-4bb0-b861-57c7c14dd0d2/download","source":{"id":"https://openalex.org/S4306400180","display_name":"UWC Research Repository (University of the Western Cape)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I869660684","host_organization_name":"University of the Western Cape","host_organization_lineage":["https://openalex.org/I869660684"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:3010173556","doi":null,"is_oa":false,"landing_page_url":"https://etd.uwc.ac.za/xmlui/bitstream/11394/7156/1/moabelo_msc_nsc_2019.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:etd.uwc.ac.za:11394/7156","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11394/7156","pdf_url":"http://hdl.handle.net/11394/7156","source":{"id":"https://openalex.org/S4306401169","display_name":"University of the Western Cape Electronic Theses and Dissertations Repository (University of the Western Cape)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I869660684","host_organization_name":"University of the Western Cape","host_organization_lineage":["https://openalex.org/I869660684"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":null},"sustainable_development_goals":[{"score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310056","display_name":"Mintek","ror":"https://ror.org/05snt2t16"},{"id":"https://openalex.org/F4320320671","display_name":"National Research Foundation","ror":"https://ror.org/05s0g1g46"},{"id":"https://openalex.org/F4320320719","display_name":"Department of Science and Technology, Ministry of Science and Technology, India","ror":"https://ror.org/0101xrq71"},{"id":"https://openalex.org/F4320334558","display_name":"Nanotechnology Innovation Centre","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3010173556.pdf","grobid_xml":"https://content.openalex.org/works/W3010173556.grobid-xml"},"referenced_works_count":160,"referenced_works":["https://openalex.org/W73211802","https://openalex.org/W195774709","https://openalex.org/W383205146","https://openalex.org/W384644027","https://openalex.org/W630365190","https://openalex.org/W647816911","https://openalex.org/W760660130","https://openalex.org/W1264225149","https://openalex.org/W1605239696","https://openalex.org/W1648483769","https://openalex.org/W1709919176","https://openalex.org/W1752045965","https://openalex.org/W1773423156","https://openalex.org/W1841822215","https://openalex.org/W1862832892","https://openalex.org/W1870388044","https://openalex.org/W1898049656","https://openalex.org/W1942296996","https://openalex.org/W1945840755","https://openalex.org/W1966508121","https://openalex.org/W1967244895","https://openalex.org/W1972722625","https://openalex.org/W1974978001","https://openalex.org/W1978765094","https://openalex.org/W1979114856","https://openalex.org/W1986199325","https://openalex.org/W1987565198","https://openalex.org/W1987566172","https://openalex.org/W1989437429","https://openalex.org/W1995141200","https://openalex.org/W1995652260","https://openalex.org/W1996819022","https://openalex.org/W1997823744","https://openalex.org/W1998662844","https://openalex.org/W1998807720","https://openalex.org/W1999781080","https://openalex.org/W2001266427","https://openalex.org/W2002865029","https://openalex.org/W2004638774","https://openalex.org/W2005595176","https://openalex.org/W2006404322","https://openalex.org/W2006782044","https://openalex.org/W2007995620","https://openalex.org/W2008797291","https://openalex.org/W2009281456","https://openalex.org/W2009968514","https://openalex.org/W2015440560","https://openalex.org/W2016340306","https://openalex.org/W2029303494","https://openalex.org/W2029984667","https://openalex.org/W2032760352","https://openalex.org/W2034354296","https://openalex.org/W2036348300","https://openalex.org/W2038730459","https://openalex.org/W2040085202","https://openalex.org/W2040399084","https://openalex.org/W2040497188","https://openalex.org/W2041268911","https://openalex.org/W2046234208","https://openalex.org/W2046313177","https://openalex.org/W2050194388","https://openalex.org/W2056511887","https://openalex.org/W2056732193","https://openalex.org/W2057694295","https://openalex.org/W2059496072","https://openalex.org/W2060705166","https://openalex.org/W2062790588","https://openalex.org/W2063814837","https://openalex.org/W2063915956","https://openalex.org/W2066670192","https://openalex.org/W2067235442","https://openalex.org/W2067812601","https://openalex.org/W2068961225","https://openalex.org/W2070665653","https://openalex.org/W2070848045","https://openalex.org/W2072418914","https://openalex.org/W2074988418","https://openalex.org/W2076092513","https://openalex.org/W2079121789","https://openalex.org/W2081270882","https://openalex.org/W2085333744","https://openalex.org/W2086047273","https://openalex.org/W2086674825","https://openalex.org/W2086841148","https://openalex.org/W2086986126","https://openalex.org/W2087225150","https://openalex.org/W2090748389","https://openalex.org/W2092136583","https://openalex.org/W2093530961","https://openalex.org/W2098169951","https://openalex.org/W2105153491","https://openalex.org/W2109587996","https://openalex.org/W2114887668","https://openalex.org/W2119342775","https://openalex.org/W2120624839","https://openalex.org/W2120753763","https://openalex.org/W2122349850","https://openalex.org/W2122772473","https://openalex.org/W2122851874","https://openalex.org/W2123585924","https://openalex.org/W2125569386","https://openalex.org/W2127675690","https://openalex.org/W2133050696","https://openalex.org/W2138761496","https://openalex.org/W2139203772","https://openalex.org/W2141259981","https://openalex.org/W2141992350","https://openalex.org/W2144392056","https://openalex.org/W2145376990","https://openalex.org/W2148152464","https://openalex.org/W2148857774","https://openalex.org/W2157121750","https://openalex.org/W2159474385","https://openalex.org/W2162869854","https://openalex.org/W2163710303","https://openalex.org/W2164427027","https://openalex.org/W2164763209","https://openalex.org/W2164779513","https://openalex.org/W2165560933","https://openalex.org/W2167277757","https://openalex.org/W2168212562","https://openalex.org/W2249715022","https://openalex.org/W2261425462","https://openalex.org/W2287510856","https://openalex.org/W2291194535","https://openalex.org/W2296585153","https://openalex.org/W2314832693","https://openalex.org/W2315963391","https://openalex.org/W2318136094","https://openalex.org/W2334366154","https://openalex.org/W2407447540","https://openalex.org/W2409505837","https://openalex.org/W2414859413","https://openalex.org/W2430132928","https://openalex.org/W2498522887","https://openalex.org/W2517645437","https://openalex.org/W2520432709","https://openalex.org/W2520903792","https://openalex.org/W2531046423","https://openalex.org/W2547782348","https://openalex.org/W2557482708","https://openalex.org/W2559600670","https://openalex.org/W2567053159","https://openalex.org/W2574588624","https://openalex.org/W2581478506","https://openalex.org/W2586645431","https://openalex.org/W2592505674","https://openalex.org/W2605136969","https://openalex.org/W2619136524","https://openalex.org/W2680362228","https://openalex.org/W2729360373","https://openalex.org/W2765368135","https://openalex.org/W2793089013","https://openalex.org/W2793263922","https://openalex.org/W2802993496","https://openalex.org/W2804063526","https://openalex.org/W2888420003","https://openalex.org/W3035579699","https://openalex.org/W3103240054","https://openalex.org/W3187362476"],"related_works":["https://openalex.org/W2944683844","https://openalex.org/W7220879461","https://openalex.org/W2770380641","https://openalex.org/W7126756939","https://openalex.org/W3214434106","https://openalex.org/W2167875539","https://openalex.org/W2985670469","https://openalex.org/W2562868867","https://openalex.org/W2600563687","https://openalex.org/W7015460175"],"abstract_inverted_index":{"Diabetes":[0],"mellitus":[1],"is":[2,35],"one":[3,467],"of":[4,41,55,60,86,99,123,141,216,229,277,288,330,336,342,364,379,397,405,412,421,429,456],"the":[5,44,53,58,87,97,124,144,163,178,198,214,217,227,258,308,365,380,383,395,410,419,447,450,453],"major":[6],"chronic":[7],"diseases":[8],"that":[9,246,296,315],"poses":[10],"a":[11,223,274,286,327,333,339,348,391,402,457],"significant":[12],"risk":[13],"to":[14,83,90,143,195,302,320,355,377,440],"human":[15],"health":[16],"globally.Current":[17],"diagnostics":[18],"for":[19,31,39,96,105,226,239,266,394,418,463],"diabetes":[20,42],"are":[21,81,436],"based":[22,416],"on":[23,382,426,446],"blood":[24],"glucose":[25],"measurements,":[26],"which":[27],"can":[28],"go":[29],"unnoticed":[30],"years.As":[32],"such,":[33],"there":[34],"an":[36,100,249,414],"urgent":[37],"need":[38],"diagnosis":[40],"at":[43,322,360],"point-of-care":[45],"(PoC),":[46],"and":[47,57,68,80,92,111,134,157,162,171,181,188,197,207,222,231,271,283,311,314,317,338,372,433,444,469],"in":[48,52,65,118,213,252,257,307,438],"low-income":[49],"settings.Recent":[50],"developments":[51],"field":[54],"nanotechnology,":[56],"use":[59],"aptamers":[61,95,114,128,142,235,242,299],"show":[62],"great":[63],"promise":[64],"developing":[66,413],"sensitive":[67],"disease-specific":[69],"assays.Aptamers":[70],"have":[71],"high":[72],"specificity,":[73],"selectivity,":[74],"low":[75],"molecular":[76],"weight,":[77],"nontoxicity,":[78],"non-immunogenicity,":[79],"easy":[82],"produce.The":[84],"aim":[85],"study":[88,408,451],"was":[89,146,193,200,264,345,353,370,399],"characterize":[91],"identify":[93],"dual":[94],"development":[98,363,455],"aptamer-based":[101],"PoC":[102],"diagnostic":[103],"kit":[104],"diabetes.Retinol":[106],"binding":[107,113,127,234,301],"protein":[108,381,388],"4":[109],"(RBP4)":[110],"its":[112],"were":[115,130,166,175,211,237],"identified":[116],"through":[117],"silico":[119],"approach.The":[120],"secondary":[121],"structures":[122],"selected":[125,238,298],"retinol":[126],"(RBA)":[129],"predicted":[131,268],"by":[132,148,168,183,202],"M-fold":[133],"Quadruplex":[135],"forming":[136],"G-Rich":[137],"Sequences":[138],"mapper.Binding":[139],"affinity":[140],"biomarker":[145],"characterized":[147,182,201],"Surface":[149],"Plasmon":[150],"Resonance(SPR),":[151],"Electrophoretic":[152],"mobility":[153],"shift":[154],"assay":[155,206,462],"(EMSA)":[156],"Enzyme-Linked":[158],"Aptamer":[159],"Assay":[160],"(ELAA)":[161],"aptamer":[164,192,415,468],"pairs":[165],"evaluated":[167],"sandwich-based":[169],"SPR":[170],"ELAA.Gold":[172],"nanoparticles":[173],"(AuNPs)":[174],"synthesized":[176],"using":[177,466],"Turkevich":[179],"method,":[180],"UV-visible":[184],"(UV-vis)":[185],"spectrophotometry,":[186],"Zetasizer":[187],"Transmission":[189],"Electron":[190],"Microscopy.One":[191],"conjugated":[194,354],"AuNPs":[196,325],"conjugate":[199],"UV-Vis,":[203],"Zetasizer,":[204],"Qubit":[205],"gel":[208],"electrophoresis.The":[209],"apt-AuNPs":[210],"used":[212],"preparation":[215],"lateral":[218,366],"flow":[219,367],"test":[220,368,448],"strips":[221],"colorimetric":[224,392,461],"aptasensor":[225,393],"detection":[228,396,403,420,465],"RBP4.RBP4":[230],"four":[232],"Retinol":[233],"(RBA's)":[236],"this":[240,373],"study.The":[241],"shared":[243,273,285],"conserved":[244,275],"motifs":[245],"might":[247],"play":[248],"ancillary":[250],"role":[251],"binding.A":[253],"small":[254],"stem":[255],"loop":[256],"nucleuotide":[259],"sequence":[260],"-TAGTAAGTGCAA-at":[261],"62-73":[262],"nts":[263],"present":[265],"all":[267,297],"structures.Furthermore,":[269],"RBA-1":[270,282,318],"RBA-3":[272],"motif":[276,287],"-GTTG-at":[278],"base":[279,290],"44-47,":[280],"whereas,":[281],"RBA-2":[284,316],"-AGGGG-at":[289],"26-32.Kinetic":[291],"studies":[292],"http://etd.uwc.ac.za/":[293],"iii":[294],"revealed":[295],"showed":[300,357],"RBP4,":[303],"with":[304,401],"dissociation":[305],"constants":[306],"nanomolar":[309],"(nM)":[310],"picomolar":[312],"range":[313],"bound":[319],"RBP4":[321,398,464],"different":[323,427],"sites.The":[324],"had":[326],"hydrodynamic":[328],"size":[329],"14":[331],"nm,":[332],"polydispersity":[334],"index":[335],"0.216":[337],"zeta":[340],"potential":[341],"-30.31":[343],"mV.RBA-2":[344],"chosen":[346],"as":[347],"capture":[349],"probe":[350],"hence":[351],"it":[352],"AuNPs.Apt-AuNPs":[356],"efficient":[358],"conjugation":[359],"50":[361],"\u00b5M.The":[362],"strip":[369],"unsuccessful":[371],"could":[374],"be":[375],"attributed":[376],"desorption":[378],"nitrocellulose":[384],"membrane":[385],"or":[386],"insufficient":[387],"concentration":[389],"used.However,":[390],"successful":[400,454],"limit":[404],"45.32":[406],"nM.This":[407],"demonstrated":[409,452],"feasibility":[411],"LFA":[417],"novel":[422],"RBP4.Therefore,":[423],"further":[424],"optimizations":[425],"types":[428],"membranes,":[430],"buffers,":[431],"pH":[432],"target":[434],"concentrations":[435],"needed":[437],"order":[439],"obtain":[441],"better":[442],"resolutions":[443],"intensity":[445],"strips.Moreover,":[449],"simple":[458],"yet":[459],"effective":[460],"AuNPs.":[470]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2020-03-13T00:00:00"}