{"id":"https://openalex.org/W3000424462","doi":"https://doi.org/10.1038/s41598-020-57500-1","title":"A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells","display_name":"A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells","publication_year":2020,"publication_date":"2020-01-20","ids":{"openalex":"https://openalex.org/W3000424462","doi":"https://doi.org/10.1038/s41598-020-57500-1","mag":"3000424462","pmid":"https://pubmed.ncbi.nlm.nih.gov/31959800","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6971260"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-020-57500-1","doi":"https://doi.org/10.1038/s41598-020-57500-1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-57500-1","pdf_url":"https://www.nature.com/articles/s41598-020-57500-1.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-020-57500-1.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049406984","display_name":"Dane Z. Hazelbaker","orcid":"https://orcid.org/0000-0001-5536-757X","observed_orcids":["https://orcid.org/0000-0001-5536-757X"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dane Z. Hazelbaker","raw_affiliation_strings":["Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037445660","display_name":"Amanda Beccard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amanda Beccard","raw_affiliation_strings":["Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034239372","display_name":"Gabriella Angelini","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gabriella Angelini","raw_affiliation_strings":["Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037105520","display_name":"Patrizia Mazzucato","orcid":"https://orcid.org/0000-0001-9446-4540","observed_orcids":["https://orcid.org/0000-0001-9446-4540"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patrizia Mazzucato","raw_affiliation_strings":["Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056276877","display_name":"Angelica Messana","orcid":"https://orcid.org/0000-0002-3484-416X","observed_orcids":["https://orcid.org/0000-0002-3484-416X"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Angelica Messana","raw_affiliation_strings":["Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111147006","display_name":"Daisy Lam","orcid":"https://orcid.org/0000-0001-8374-8409","observed_orcids":["https://orcid.org/0000-0001-8374-8409"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daisy Lam","raw_affiliation_strings":["Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064730165","display_name":"Kevin C. Eggan","orcid":"https://orcid.org/0000-0003-4436-8467","observed_orcids":["https://orcid.org/0000-0003-4436-8467"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin Eggan","raw_affiliation_strings":["Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA","Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052355287","display_name":"Lindy E. Barrett","orcid":"https://orcid.org/0000-0002-3229-4774","observed_orcids":["https://orcid.org/0000-0002-3229-4774"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4392738323","display_name":"Stanley Center for Psychiatric Research","ror":"https://ror.org/01tm9b530","country_code":"US","type":"facility","lineage":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Lindy E. Barrett","raw_affiliation_strings":["Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA. lbarrett@broadinstitute.org","Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA. lbarrett@broadinstitute.org","Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA","Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA. lbarrett@broadinstitute.org","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA. lbarrett@broadinstitute.org","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]},{"raw_affiliation_string":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I4392738323"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5052355287"],"corresponding_institution_ids":["https://openalex.org/I107606265","https://openalex.org/I136199984","https://openalex.org/I4392738323"],"apc_list":{"value":1870,"currency":"USD","value_usd":1870},"apc_paid":{"value":1870,"currency":"USD","value_usd":1870},"fwci":1.6437,"has_fulltext":true,"cited_by_count":40,"citation_normalized_percentile":{"value":0.83700398,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"10","issue":"1","first_page":"635","last_page":"635"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/piggybac-transposon","display_name":"piggyBac transposon","score":0.9369999766349792},{"id":"https://openalex.org/keywords/crispr-interference","display_name":"CRISPR interference","score":0.8560000061988831},{"id":"https://openalex.org/keywords/crispr-activation","display_name":"CRISPR activation","score":0.9980000257492065},{"id":"https://openalex.org/keywords/dcas9","display_name":"dCas9","score":0.8519999980926514},{"id":"https://openalex.org/keywords/human-pluripotent-stem-cells","display_name":"human pluripotent stem cells","score":0.9200000166893005},{"id":"https://openalex.org/keywords/dcas9-krab","display_name":"dCas9-KRAB","score":0.871999979019165},{"id":"https://openalex.org/keywords/tcf4","display_name":"TCF4","score":0.9639999866485596},{"id":"https://openalex.org/keywords/genome-wide-screen","display_name":"genome-wide screen","score":0.7829999923706055},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.4449999928474426}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8669037818908691},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.7693008184432983},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.7452433705329895},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6899051666259766},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.6696438193321228},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.6556168794631958},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.6177830696105957},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6102704405784607},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.5077327489852905},{"id":"https://openalex.org/C84356384","wikidata":"https://www.wikidata.org/wiki/Q7833575","display_name":"Trans-activating crRNA","level":5,"score":0.49412277340888977},{"id":"https://openalex.org/C31909778","wikidata":"https://www.wikidata.org/wiki/Q7833921","display_name":"Transcription activator-like effector nuclease","level":5,"score":0.4326778054237366},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3594071865081787},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3089296817779541},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.28460127115249634},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.23446741700172424}],"mesh":[{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004251","descriptor_name":"DNA Transposable Elements","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029721","descriptor_name":"Drosophila Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D039904","descriptor_name":"Pluripotent Stem Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051792","descriptor_name":"Basic Helix-Loop-Helix Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1038/s41598-020-57500-1","doi":"https://doi.org/10.1038/s41598-020-57500-1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-57500-1","pdf_url":"https://www.nature.com/articles/s41598-020-57500-1.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"doi:10.1101/603951","doi":"https://doi.org/10.1101/603951","is_oa":true,"landing_page_url":"https://doi.org/10.1101/603951","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/04/09/603951.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:6971260","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6971260","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:31959800","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31959800","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:dcafdfc80ecc4eccad08940635009d53","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/dcafdfc80ecc4eccad08940635009d53","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1038/s41598-020-57500-1","doi":"https://doi.org/10.1038/s41598-020-57500-1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-020-57500-1","pdf_url":"https://www.nature.com/articles/s41598-020-57500-1.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.428600013256073,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320314070","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142"},{"id":"https://openalex.org/F4320323438","display_name":"Tallinna Tehnika\u00fclikool","ror":"https://ror.org/0443cwa12"},{"id":"https://openalex.org/F4320333423","display_name":"Stanley Center for Psychiatric Research, Broad Institute","ror":"https://ror.org/01tm9b530"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3000424462.pdf","grobid_xml":"https://content.openalex.org/works/W3000424462.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1769569140","https://openalex.org/W1833135536","https://openalex.org/W1967841384","https://openalex.org/W1984359015","https://openalex.org/W2040479298","https://openalex.org/W2049948975","https://openalex.org/W2070134501","https://openalex.org/W2070172761","https://openalex.org/W2077814724","https://openalex.org/W2086943348","https://openalex.org/W2089624112","https://openalex.org/W2101357408","https://openalex.org/W2101749257","https://openalex.org/W2114232807","https://openalex.org/W2117924928","https://openalex.org/W2128551987","https://openalex.org/W2142077292","https://openalex.org/W2152505144","https://openalex.org/W2158979552","https://openalex.org/W2163173465","https://openalex.org/W2284442248","https://openalex.org/W2295326929","https://openalex.org/W2414684615","https://openalex.org/W2461493169","https://openalex.org/W2468765937","https://openalex.org/W2524507363","https://openalex.org/W2554763217","https://openalex.org/W2558758033","https://openalex.org/W2560890620","https://openalex.org/W2624845852","https://openalex.org/W2736544909","https://openalex.org/W2741244157","https://openalex.org/W2752453466","https://openalex.org/W2763110592","https://openalex.org/W2765888851","https://openalex.org/W2772524563","https://openalex.org/W2783322400","https://openalex.org/W2803567054","https://openalex.org/W2803581084","https://openalex.org/W2807198924","https://openalex.org/W2883377762","https://openalex.org/W2883697525","https://openalex.org/W2896045573","https://openalex.org/W2911701742","https://openalex.org/W2914691622","https://openalex.org/W2952044849","https://openalex.org/W4243162360"],"related_works":["https://openalex.org/W4210494299","https://openalex.org/W2624845852","https://openalex.org/W2952817890","https://openalex.org/W2952044849","https://openalex.org/W3017493802","https://openalex.org/W2894935582","https://openalex.org/W3184902800","https://openalex.org/W2124758693","https://openalex.org/W3194508928","https://openalex.org/W2615888080"],"abstract_inverted_index":{"CRISPR-Cas9-mediated":[0],"gene":[1,12,119],"interference":[2],"(CRISPRi)":[3],"and":[4,14,44,56,61,72,87,90,98,108,122,132,203,214],"activation":[5],"(CRISPRa)":[6],"approaches":[7],"hold":[8],"promise":[9],"for":[10,95,144,151,210],"functional":[11,198],"studies":[13,179,211],"genome-wide":[15],"screens":[16,209],"in":[17,24,120,180],"human":[18],"pluripotent":[19],"stem":[20],"cells":[21,101],"(hPSCs).":[22],"However,":[23],"contrast":[25],"to":[26,69,93,115,174],"CRISPR-Cas9":[27],"nuclease":[28],"approaches,":[29],"the":[30,39,105,117,127,138],"efficiency":[31],"of":[32,38,75,100,126,140,161,200,212],"CRISPRi/a":[33,191],"depends":[34],"on":[35,53],"continued":[36],"expression":[37,74,124],"dead":[40],"Cas9":[41],"(dCas9)":[42],"effector":[43],"guide":[45],"RNA":[46],"(gRNA),":[47],"which":[48],"can":[49,170,196],"vary":[50],"substantially":[51],"depending":[52],"transgene":[54],"design":[55,60],"delivery.":[57],"Here,":[58],"we":[59,80],"generate":[62,81],"new":[63],"fluorescently":[64],"labeled":[65],"piggyBac":[66],"(PB)":[67],"vectors":[68],"deliver":[70],"uniform":[71],"sustained":[73],"multiplexed":[76,164],"gRNAs.":[77,109],"In":[78],"addition,":[79],"hPSC":[82],"lines":[83],"harboring":[84],"AAVS1-integrated,":[85],"inducible":[86],"fluorescent":[88],"dCas9-KRAB":[89],"dCas9-VPR":[91],"transgenes":[92],"allow":[94],"accurate":[96],"quantification":[97],"tracking":[99],"that":[102,169],"express":[103],"both":[104],"dCas9":[106,128],"effectors":[107],"We":[110,135],"then":[111],"employ":[112],"these":[113],"systems":[114],"target":[116],"TCF4":[118],"hPSCs":[121],"assess":[123,137],"levels":[125],"effectors,":[129],"individual":[130,201],"gRNAs":[131],"targeted":[133],"gene.":[134],"also":[136],"performance":[139],"our":[141,156,188],"PB":[142,165],"system":[143,168,195],"single":[145],"gRNA":[146,166],"delivery,":[147],"confirming":[148],"its":[149],"utility":[150],"library":[152],"format":[153],"applications.":[154],"Collectively,":[155],"results":[157],"provide":[158],"proof-of-principle":[159],"application":[160],"a":[162],"stable,":[163],"delivery":[167],"be":[171],"widely":[172],"exploited":[173],"further":[175],"enable":[176],"genome":[177],"engineering":[178],"hPSCs.":[181],"Paired":[182],"with":[183],"diverse":[184],"CRISPR":[185],"tools":[186],"including":[187],"dual":[189],"fluorescence":[190],"cell":[192],"lines,":[193],"this":[194],"facilitate":[197],"dissection":[199],"genes":[202],"pathways":[204],"as":[205,207],"well":[206],"larger-scale":[208],"development":[213],"disease.":[215]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":10},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2020-01-23T00:00:00"}
