{"id":"https://openalex.org/W3000200402","doi":"https://doi.org/10.1021/acs.jafc.9b07290","title":"Harnessing Yeast Peroxisomes and Cytosol Acetyl-CoA for Sesquiterpene \u03b1-Humulene Production","display_name":"Harnessing Yeast Peroxisomes and Cytosol Acetyl-CoA for Sesquiterpene \u03b1-Humulene Production","publication_year":2020,"publication_date":"2020-01-16","ids":{"openalex":"https://openalex.org/W3000200402","doi":"https://doi.org/10.1021/acs.jafc.9b07290","mag":"3000200402","pmid":"https://pubmed.ncbi.nlm.nih.gov/31944688"},"language":"en","primary_location":{"id":"doi:10.1021/acs.jafc.9b07290","doi":"https://doi.org/10.1021/acs.jafc.9b07290","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.jafc.9b07290","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W3000200402"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5069729806","display_name":"Chuanbo Zhang","orcid":"https://orcid.org/0000-0002-2550-8279","observed_orcids":["https://orcid.org/0000-0002-2550-8279"]},"institutions":[{"id":"https://openalex.org/I162868743","display_name":"Tianjin University","ror":"https://ror.org/012tb2g32","country_code":"CN","type":"education","lineage":["https://openalex.org/I162868743"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chuanbo Zhang","raw_affiliation_strings":["School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I162868743"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100404490","display_name":"Man Li","orcid":"https://orcid.org/0000-0002-7133-9138","observed_orcids":["https://orcid.org/0000-0002-7133-9138"]},"institutions":[{"id":"https://openalex.org/I162868743","display_name":"Tianjin University","ror":"https://ror.org/012tb2g32","country_code":"CN","type":"education","lineage":["https://openalex.org/I162868743"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Man Li","raw_affiliation_strings":["School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I162868743"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101754857","display_name":"Guang-Rong Zhao","orcid":"https://orcid.org/0000-0002-6881-9042","observed_orcids":["https://orcid.org/0000-0002-6881-9042"]},"institutions":[{"id":"https://openalex.org/I162868743","display_name":"Tianjin University","ror":"https://ror.org/012tb2g32","country_code":"CN","type":"education","lineage":["https://openalex.org/I162868743"]},{"id":"https://openalex.org/I4210151209","display_name":"Collaborative Innovation Center of Chemical Science and Engineering Tianjin","ror":"https://ror.org/0225a5s12","country_code":"CN","type":"facility","lineage":["https://openalex.org/I162868743","https://openalex.org/I205237279","https://openalex.org/I4210151209"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guang-Rong Zhao","raw_affiliation_strings":["Key Laboratory of System Bioengineering, Ministry of Education Tianjin University, Tianjin 300350, People\u2019s Republic of China","School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China","SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300350, People\u2019s Republic of China"],"raw_orcid":"https://orcid.org/0000-0002-6881-9042","affiliations":[{"raw_affiliation_string":"Key Laboratory of System Bioengineering, Ministry of Education Tianjin University, Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I162868743"]},{"raw_affiliation_string":"School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I162868743"]},{"raw_affiliation_string":"SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I4210151209"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019739656","display_name":"Wenyu Lu","orcid":"https://orcid.org/0000-0001-5098-6697","observed_orcids":["https://orcid.org/0000-0001-5098-6697","https://orcid.org/0000-0002-4910-4025"]},"institutions":[{"id":"https://openalex.org/I162868743","display_name":"Tianjin University","ror":"https://ror.org/012tb2g32","country_code":"CN","type":"education","lineage":["https://openalex.org/I162868743"]},{"id":"https://openalex.org/I4210151209","display_name":"Collaborative Innovation Center of Chemical Science and Engineering Tianjin","ror":"https://ror.org/0225a5s12","country_code":"CN","type":"facility","lineage":["https://openalex.org/I162868743","https://openalex.org/I205237279","https://openalex.org/I4210151209"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Wenyu Lu","raw_affiliation_strings":["Key Laboratory of System Bioengineering, Ministry of Education Tianjin University, Tianjin 300350, People\u2019s Republic of China","School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China","SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300350, People\u2019s Republic of China"],"raw_orcid":"https://orcid.org/0000-0001-5098-6697","affiliations":[{"raw_affiliation_string":"Key Laboratory of System Bioengineering, Ministry of Education Tianjin University, Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I162868743"]},{"raw_affiliation_string":"School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I162868743"]},{"raw_affiliation_string":"SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300350, People\u2019s Republic of China","institution_ids":["https://openalex.org/I4210151209"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5019739656"],"corresponding_institution_ids":["https://openalex.org/I162868743","https://openalex.org/I4210151209"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":3.2159,"has_fulltext":false,"cited_by_count":86,"citation_normalized_percentile":{"value":0.93926191,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":100},"biblio":{"volume":"68","issue":"5","first_page":"1382","last_page":"1389"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.57669997215271,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.57669997215271,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.4065000116825104,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14150","display_name":"Biochemical and biochemical processes","score":0.006200000178068876,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-humulene","display_name":"\u03b1-humulene","score":0.9750000238418579},{"id":"https://openalex.org/keywords/sesquiterpenes","display_name":"sesquiterpenes","score":0.9380000233650208},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9150000214576721},{"id":"https://openalex.org/keywords/peroxisomes","display_name":"peroxisomes","score":0.8740000128746033},{"id":"https://openalex.org/keywords/acetyl-coa","display_name":"acetyl-CoA","score":0.7940000295639038},{"id":"https://openalex.org/keywords/terpenoid-production","display_name":"terpenoid production","score":0.8130000233650208},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.734000027179718}],"concepts":[{"id":"https://openalex.org/C127078168","wikidata":"https://www.wikidata.org/wiki/Q138639","display_name":"Peroxisome","level":3,"score":0.8177172541618347},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.7076460123062134},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.7068442106246948},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6762560606002808},{"id":"https://openalex.org/C2777291603","wikidata":"https://www.wikidata.org/wiki/Q1995108","display_name":"Humulene","level":3,"score":0.6419904828071594},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.6114132404327393},{"id":"https://openalex.org/C206103511","wikidata":"https://www.wikidata.org/wiki/Q426694","display_name":"Terpenoid","level":2,"score":0.5947372317314148},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.5657750964164734},{"id":"https://openalex.org/C2775933503","wikidata":"https://www.wikidata.org/wiki/Q25113781","display_name":"Farnesyl diphosphate synthase","level":4,"score":0.5655534863471985},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.5337731838226318},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4259827435016632},{"id":"https://openalex.org/C168170006","wikidata":"https://www.wikidata.org/wiki/Q864699","display_name":"Bioreactor","level":2,"score":0.41118061542510986},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38731324672698975},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.35030221939086914},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.17294606566429138},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1435530185699463},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09907481074333191},{"id":"https://openalex.org/C2777278459","wikidata":"https://www.wikidata.org/wiki/Q170885","display_name":"Essential oil","level":2,"score":0.09184420108795166}],"mesh":[{"descriptor_ui":"D000081222","descriptor_name":"Monocyclic Sesquiterpenes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000105","descriptor_name":"Acetyl Coenzyme A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012717","descriptor_name":"Sesquiterpenes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020675","descriptor_name":"Peroxisomes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020675","descriptor_name":"Peroxisomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/acs.jafc.9b07290","doi":"https://doi.org/10.1021/acs.jafc.9b07290","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.jafc.9b07290","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"},{"id":"pmid:31944688","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31944688","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of agricultural and food chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8958942404","display_name":"\u4ee3\u8c22\u6728\u7cd6\u5408\u6210\u4eba\u53c2\u7682\u82f7\u917f\u9152\u9175\u6bcd\u6784\u5efa\u53ca\u8c03\u63a7\u673a\u5236\u7814\u7a76","funder_award_id":"21878220","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W1882032094","https://openalex.org/W1967363201","https://openalex.org/W1975432121","https://openalex.org/W1977933239","https://openalex.org/W1980196761","https://openalex.org/W1982669711","https://openalex.org/W1985217551","https://openalex.org/W1987039237","https://openalex.org/W1991197152","https://openalex.org/W1994675489","https://openalex.org/W2000625643","https://openalex.org/W2001687838","https://openalex.org/W2014968469","https://openalex.org/W2030168212","https://openalex.org/W2038591876","https://openalex.org/W2047451100","https://openalex.org/W2049039071","https://openalex.org/W2071838425","https://openalex.org/W2073480839","https://openalex.org/W2077997117","https://openalex.org/W2078390802","https://openalex.org/W2092122738","https://openalex.org/W2092167045","https://openalex.org/W2098390695","https://openalex.org/W2109910682","https://openalex.org/W2125130629","https://openalex.org/W2136018257","https://openalex.org/W2146073809","https://openalex.org/W2157667687","https://openalex.org/W2161257110","https://openalex.org/W2164110299","https://openalex.org/W2324868592","https://openalex.org/W2410761017","https://openalex.org/W2485902845","https://openalex.org/W2511727539","https://openalex.org/W2587955894","https://openalex.org/W2606427413","https://openalex.org/W2606816949","https://openalex.org/W2626617466","https://openalex.org/W2738089584","https://openalex.org/W2883884122","https://openalex.org/W2947525207","https://openalex.org/W4211259186","https://openalex.org/W4214661561"],"related_works":["https://openalex.org/W7163818328","https://openalex.org/W4284890723","https://openalex.org/W3214332783","https://openalex.org/W2988978830","https://openalex.org/W4281570193","https://openalex.org/W4386091429","https://openalex.org/W2976236040","https://openalex.org/W4312203332","https://openalex.org/W2116063575","https://openalex.org/W7152955175"],"abstract_inverted_index":{"Metabolic":[0],"engineering":[1,17],"of":[2,71,93],"Saccharomyces":[3],"cerevisiae":[4,40,119],"focusing":[5],"on":[6],"the":[7,29,64,69,90],"cytoplasm":[8],"for":[9,19,114],"sustainable":[10],"terpenoid":[11,20,115],"production":[12,21,60,77,116],"is":[13,22],"commonly":[14],"practiced.":[15],"However,":[16],"organelles":[18],"rarely":[23],"reported.":[24],"Herein,":[25],"peroxisomes,":[26],"together":[27],"with":[28,61],"cytoplasm,":[30],"were":[31,50],"engineered":[32],"to":[33,56,83],"boost":[34],"sesquiterpene":[35],"\u03b1-humulene":[36,48,59,76,91],"synthesis":[37],"in":[38,85,101,117],"S.":[39,118],".":[41,120],"The":[42,106],"farnesyl":[43],"diphosphate":[44],"synthetic":[45],"pathway":[46],"and":[47,73],"synthase":[49],"successfully":[51],"expressed":[52],"inside":[53],"yeast":[54],"peroxisomes":[55],"enable":[57],"high-level":[58],"glucose":[62],"as":[63],"sole":[65],"carbon":[66],"source.":[67],"With":[68],"combination":[70],"peroxisomal":[72],"cytoplasmic":[74],"engineering,":[75],"was":[78,96],"increased":[79],"by":[80,98],"2.5-fold":[81],"compared":[82],"that":[84],"cytoplasm-engineered":[86],"recombinant":[87],"strains.":[88],"Finally,":[89],"titer":[92],"1726.78":[94],"mg/L":[95],"achieved":[97],"fed-batch":[99],"fermentation":[100],"a":[102],"5":[103],"L":[104],"bioreactor.":[105],"strategy":[107],"presented":[108],"here":[109],"offers":[110],"an":[111],"efficient":[112],"method":[113]},"counts_by_year":[{"year":2026,"cited_by_count":8},{"year":2025,"cited_by_count":12},{"year":2024,"cited_by_count":21},{"year":2023,"cited_by_count":10},{"year":2022,"cited_by_count":18},{"year":2021,"cited_by_count":13},{"year":2020,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2020-01-23T00:00:00"}