{"id":"https://openalex.org/W2999860050","doi":"https://doi.org/10.1002/prca.201900060","title":"A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next\u2010Generation Sequencing","display_name":"A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next\u2010Generation Sequencing","publication_year":2020,"publication_date":"2020-01-17","ids":{"openalex":"https://openalex.org/W2999860050","doi":"https://doi.org/10.1002/prca.201900060","mag":"2999860050","pmid":"https://pubmed.ncbi.nlm.nih.gov/31950679"},"language":"en","primary_location":{"id":"doi:10.1002/prca.201900060","doi":"https://doi.org/10.1002/prca.201900060","is_oa":true,"landing_page_url":"https://doi.org/10.1002/prca.201900060","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/prca.201900060","source":{"id":"https://openalex.org/S118886446","display_name":"PROTEOMICS - CLINICAL APPLICATIONS","issn_l":"1862-8346","issn":["1862-8346","1862-8354"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PROTEOMICS \u2013 Clinical Applications","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/prca.201900060","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061832261","display_name":"Daniel Manoil","orcid":"https://orcid.org/0000-0002-9220-3977","observed_orcids":["https://orcid.org/0000-0002-9220-3977"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Daniel Manoil","raw_affiliation_strings":["Division of Oral Diseases, Department of Dental Medicine, Karolinska Institute, Huddinge, 14152 Sweden"],"raw_orcid":"https://orcid.org/0000-0002-9220-3977","affiliations":[{"raw_affiliation_string":"Division of Oral Diseases, Department of Dental Medicine, Karolinska Institute, Huddinge, 14152 Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014943241","display_name":"Khaled Al\u2010Manei","orcid":"https://orcid.org/0000-0003-0787-5849","observed_orcids":["https://orcid.org/0000-0003-0787-5849"]},"institutions":[{"id":"https://openalex.org/I28022161","display_name":"King Saud University","ror":"https://ror.org/02f81g417","country_code":"SA","type":"education","lineage":["https://openalex.org/I28022161"]},{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SA","SE"],"is_corresponding":false,"raw_author_name":"Khaled Al\u2010Manei","raw_affiliation_strings":["Division of Endodontics, Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh, 11545 Saudi Arabia","Division of Oral Diseases, Department of Dental Medicine, Karolinska Institute, Huddinge, 14152 Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Endodontics, Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh, 11545 Saudi Arabia","institution_ids":["https://openalex.org/I28022161"]},{"raw_affiliation_string":"Division of Oral Diseases, Department of Dental Medicine, Karolinska Institute, Huddinge, 14152 Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073538546","display_name":"Georgios N. Belibasakis","orcid":"https://orcid.org/0000-0002-8164-0653","observed_orcids":["https://orcid.org/0000-0002-8164-0653"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Georgios N. Belibasakis","raw_affiliation_strings":["Division of Oral Diseases, Department of Dental Medicine, Karolinska Institute, Huddinge, 14152 Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Oral Diseases, Department of Dental Medicine, Karolinska Institute, Huddinge, 14152 Sweden","institution_ids":["https://openalex.org/I28166907"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5061832261"],"corresponding_institution_ids":["https://openalex.org/I28166907"],"apc_list":{"value":3800,"currency":"USD","value_usd":3800},"apc_paid":{"value":3800,"currency":"USD","value_usd":3800},"fwci":4.2784,"has_fulltext":true,"cited_by_count":112,"citation_normalized_percentile":{"value":0.96642686,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"14","issue":"3","first_page":"e1900060","last_page":"e1900060"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10536","display_name":"Endodontics and Root Canal Treatments","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/3504","display_name":"Oral Surgery"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10536","display_name":"Endodontics and Root Canal Treatments","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/3504","display_name":"Oral Surgery"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10257","display_name":"Oral microbiology and periodontitis research","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/3506","display_name":"Periodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11551","display_name":"Infective Endocarditis Diagnosis and Management","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/root-canal-microbiota","display_name":"root canal microbiota","score":0.9789999723434448},{"id":"https://openalex.org/keywords/apical-periodontitis","display_name":"apical periodontitis","score":0.9950000047683716},{"id":"https://openalex.org/keywords/apical-abscess","display_name":"apical abscess","score":0.9980000257492065},{"id":"https://openalex.org/keywords/endodontic-infection","display_name":"root canal infection","score":0.9819999933242798},{"id":"https://openalex.org/keywords/microbial-community-composition","display_name":"microbial community composition","score":0.8140000104904175},{"id":"https://openalex.org/keywords/bacterial-taxa-abundance","display_name":"bacterial taxa abundance","score":0.5929999947547913},{"id":"https://openalex.org/keywords/differential-abundance-analysis","display_name":"differential abundance","score":0.453000009059906}],"concepts":[{"id":"https://openalex.org/C2780385504","wikidata":"https://www.wikidata.org/wiki/Q520127","display_name":"Periodontitis","level":2,"score":0.7755852341651917},{"id":"https://openalex.org/C2779747767","wikidata":"https://www.wikidata.org/wiki/Q848390","display_name":"Root canal","level":2,"score":0.662431001663208},{"id":"https://openalex.org/C199343813","wikidata":"https://www.wikidata.org/wiki/Q12128","display_name":"Dentistry","level":1,"score":0.4925580620765686},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44913309812545776},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.41866374015808105},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.36333954334259033},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.21518626809120178},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10782918334007263}],"mesh":[{"descriptor_ui":"D003786","descriptor_name":"Dental Pulp Cavity","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010485","descriptor_name":"Periapical Periodontitis","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064307","descriptor_name":"Microbiota","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1002/prca.201900060","doi":"https://doi.org/10.1002/prca.201900060","is_oa":true,"landing_page_url":"https://doi.org/10.1002/prca.201900060","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/prca.201900060","source":{"id":"https://openalex.org/S118886446","display_name":"PROTEOMICS - CLINICAL APPLICATIONS","issn_l":"1862-8346","issn":["1862-8346","1862-8354"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PROTEOMICS \u2013 Clinical Applications","raw_type":"journal-article"},{"id":"pmid:31950679","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31950679","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteomics. Clinical applications","raw_type":"Journal Article"},{"id":"pmh:oai:unige.ch:aou:unige:164784","doi":null,"is_oa":true,"landing_page_url":"https://archive-ouverte.unige.ch/unige:164784","pdf_url":null,"source":{"id":"https://openalex.org/S4306402259","display_name":"Archive ouverte UNIGE (University of Geneva)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114457229","host_organization_name":"University of Geneva","host_organization_lineage":["https://openalex.org/I114457229"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ISSN: 1862-8346","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:unige.ch:unige:164784","doi":null,"is_oa":true,"landing_page_url":"https://archive-ouverte.unige.ch/unige:164784","pdf_url":null,"source":{"id":"https://openalex.org/S4306402259","display_name":"Archive ouverte UNIGE (University of Geneva)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114457229","host_organization_name":"University of Geneva","host_organization_lineage":["https://openalex.org/I114457229"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ISSN: 1862-8346","raw_type":"info:eu-repo/semantics/review"}],"best_oa_location":{"id":"doi:10.1002/prca.201900060","doi":"https://doi.org/10.1002/prca.201900060","is_oa":true,"landing_page_url":"https://doi.org/10.1002/prca.201900060","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/prca.201900060","source":{"id":"https://openalex.org/S118886446","display_name":"PROTEOMICS - CLINICAL APPLICATIONS","issn_l":"1862-8346","issn":["1862-8346","1862-8354"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PROTEOMICS \u2013 Clinical Applications","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9763000011444092}],"study_designs":[{"display_name":"Systematic Review","id":"https://openalex.org/study-designs/systematic-review"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320322315","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691"},{"id":"https://openalex.org/F4320328806","display_name":"Janggen-P\u00f6hn-Stiftung","ror":"https://ror.org/04fegx276"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2999860050.pdf","grobid_xml":"https://content.openalex.org/works/W2999860050.grobid-xml"},"referenced_works_count":116,"referenced_works":["https://openalex.org/W1791491556","https://openalex.org/W1911106917","https://openalex.org/W1964975641","https://openalex.org/W1972430769","https://openalex.org/W1974722196","https://openalex.org/W1976836197","https://openalex.org/W1981659331","https://openalex.org/W1982392765","https://openalex.org/W1982879979","https://openalex.org/W1985197139","https://openalex.org/W1992569019","https://openalex.org/W1993319393","https://openalex.org/W1998377233","https://openalex.org/W1999713271","https://openalex.org/W2001128292","https://openalex.org/W2004534384","https://openalex.org/W2012911819","https://openalex.org/W2017529960","https://openalex.org/W2018778348","https://openalex.org/W2019094601","https://openalex.org/W2024852896","https://openalex.org/W2026427346","https://openalex.org/W2028398950","https://openalex.org/W2029088033","https://openalex.org/W2031011778","https://openalex.org/W2033721272","https://openalex.org/W2045566769","https://openalex.org/W2049746328","https://openalex.org/W2052362064","https://openalex.org/W2055631148","https://openalex.org/W2057664891","https://openalex.org/W2060242040","https://openalex.org/W2060935193","https://openalex.org/W2061674177","https://openalex.org/W2062030121","https://openalex.org/W2063276097","https://openalex.org/W2064675571","https://openalex.org/W2065361712","https://openalex.org/W2066295973","https://openalex.org/W2074430065","https://openalex.org/W2074598906","https://openalex.org/W2080843336","https://openalex.org/W2083818621","https://openalex.org/W2087671769","https://openalex.org/W2090055375","https://openalex.org/W2093873659","https://openalex.org/W2098923148","https://openalex.org/W2100230373","https://openalex.org/W2101294025","https://openalex.org/W2101866347","https://openalex.org/W2103707133","https://openalex.org/W2103805072","https://openalex.org/W2104348453","https://openalex.org/W2106464940","https://openalex.org/W2107119891","https://openalex.org/W2108786601","https://openalex.org/W2114070247","https://openalex.org/W2114703854","https://openalex.org/W2117522399","https://openalex.org/W2123452260","https://openalex.org/W2123682752","https://openalex.org/W2127723704","https://openalex.org/W2127971081","https://openalex.org/W2129218043","https://openalex.org/W2137977329","https://openalex.org/W2139550762","https://openalex.org/W2142250645","https://openalex.org/W2144069576","https://openalex.org/W2146227876","https://openalex.org/W2150821483","https://openalex.org/W2152984372","https://openalex.org/W2154712040","https://openalex.org/W2156098321","https://openalex.org/W2158475607","https://openalex.org/W2164784818","https://openalex.org/W2165840272","https://openalex.org/W2167956476","https://openalex.org/W2167982785","https://openalex.org/W2169474803","https://openalex.org/W2170486072","https://openalex.org/W2196174506","https://openalex.org/W2262155359","https://openalex.org/W2269786471","https://openalex.org/W2293032852","https://openalex.org/W2394897061","https://openalex.org/W2396849069","https://openalex.org/W2418413361","https://openalex.org/W2527409327","https://openalex.org/W2537617673","https://openalex.org/W2603346833","https://openalex.org/W2611957242","https://openalex.org/W2714233250","https://openalex.org/W2762425175","https://openalex.org/W2763540102","https://openalex.org/W2782270452","https://openalex.org/W2800683724","https://openalex.org/W2803377090","https://openalex.org/W2806056217","https://openalex.org/W2809073263","https://openalex.org/W2884441543","https://openalex.org/W2897700370","https://openalex.org/W2898868157","https://openalex.org/W2902943436","https://openalex.org/W2912323350","https://openalex.org/W2937731684","https://openalex.org/W2972720814","https://openalex.org/W4294215472","https://openalex.org/W6600753396","https://openalex.org/W6601711932","https://openalex.org/W6635274082","https://openalex.org/W6643797680","https://openalex.org/W6698679425","https://openalex.org/W6732486816","https://openalex.org/W6746591711","https://openalex.org/W6814943250","https://openalex.org/W6821699096"],"related_works":["https://openalex.org/W7214140039","https://openalex.org/W2803377090","https://openalex.org/W7154486362","https://openalex.org/W4311277697","https://openalex.org/W4317756929","https://openalex.org/W3188895823","https://openalex.org/W2807046397","https://openalex.org/W4387696322","https://openalex.org/W4394914928","https://openalex.org/W3039478073"],"abstract_inverted_index":{"Next-generation":[0],"sequencing":[1,118],"(NGS)":[2],"has":[3],"now":[4],"been":[5],"applied":[6],"for":[7,112],"a":[8,65,145,175],"decade":[9],"to":[10,197],"characterize":[11],"the":[12,25,36,41,55,58,76,90,102,113,164,179],"microbiota":[13,42,91,121,165],"composition":[14,43],"of":[15,38,67,92,97,163,178,188],"infected":[16,93],"dental":[17],"root":[18],"canals":[19],"associated":[20,143,181],"with":[21,144,182],"apical":[22,128,132,136],"periodontitis.":[23],"Here,":[24],"study":[26],"aims":[27],"at":[28],"systematically":[29],"and":[30,57,110,120,135,158,185],"critically":[31],"reviewing":[32],"these":[33],"reports":[34],"within":[35],"outcome":[37],"interest":[39],"selected;":[40],"in":[44],"different":[45],"endodontic":[46,124,183],"infection":[47,140,167],"types.":[48],"Standard":[49],"methodological":[50],"guidelines":[51],"as":[52],"stated":[53],"by":[54,95,166],"PRISMA":[56],"Joanna":[59],"Briggs":[60],"Institute":[61],"are":[62,99,108,142],"followed,":[63],"including":[64],"risk":[66],"bias":[68],"assessment.":[69],"A":[70],"literature":[71],"search":[72],"is":[73,169],"conducted":[74],"using":[75],"PubMed":[77],"Advanced-Search":[78],"Builder":[79],"on":[80],"April":[81],"8,":[82],"2019;":[83],"only":[84],"original":[85],"research":[86],"articles":[87,104],"that":[88],"investigated":[89],"root-canals":[94],"means":[96],"NGS":[98],"screened.":[100],"Among":[101],"26":[103],"initially":[105],"identified,":[106],"18":[107],"included":[109],"evaluated":[111],"following":[114],"parameters;":[115],"sampling":[116],"protocol,":[117],"strategy,":[119],"composition.":[122],"The":[123],"infections":[125,184],"include":[126],"primary":[127],"periodontitis":[129,133],"(PAP),":[130],"secondary":[131],"(SAP),":[134],"abscess":[137],"(AA).":[138],"All":[139],"types":[141],"highly":[146],"diverse":[147],"microbiota.":[148],"Although":[149],"some":[150],"taxa":[151,180],"appear":[152],"differentially":[153],"abundant":[154],"between":[155],"PAPs,":[156],"SAPs,":[157],"AAs,":[159],"no":[160],"evident":[161],"clustering":[162],"type":[168],"observed.":[170],"These":[171],"studies":[172],"collectively":[173],"formulate":[174],"comprehensive":[176],"map":[177],"provide":[186],"evidence":[187],"compositionally":[189],"unspecific,":[190],"yet":[191],"abundance":[192],"differentiates,":[193],"community":[194],"profiles":[195],"according":[196],"clinical":[198],"diagnosis.":[199]},"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":13},{"year":2024,"cited_by_count":18},{"year":2023,"cited_by_count":25},{"year":2022,"cited_by_count":23},{"year":2021,"cited_by_count":13},{"year":2020,"cited_by_count":11}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2020-01-23T00:00:00"}