{"id":"https://openalex.org/W2999237457","doi":null,"title":"Quantifying Expression of Interneuron Subtype Markers for Dlx-2 Transfected NG2 Cells","display_name":"Quantifying Expression of Interneuron Subtype Markers for Dlx-2 Transfected NG2 Cells","publication_year":2019,"publication_date":"2019-01-01","ids":{"openalex":"https://openalex.org/W2999237457","mag":"2999237457"},"language":"en","primary_location":{"id":"pmh:oai:opencommons.uconn.edu:srhonors_theses-1657","doi":null,"is_oa":true,"landing_page_url":"https://opencommons.uconn.edu/srhonors_theses/647","pdf_url":"https://digitalcommons.lib.uconn.edu/cgi/viewcontent.cgi?article=1657&context=srhonors_theses","source":{"id":"https://openalex.org/S4306401180","display_name":"OpenCommons at University of Connecticut (University of Connecticut)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I140172145","host_organization_name":"University of Connecticut","host_organization_lineage":["https://openalex.org/I140172145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Honors Scholar Theses","raw_type":"text"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://digitalcommons.lib.uconn.edu/cgi/viewcontent.cgi?article=1657&context=srhonors_theses","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109806931","display_name":"Timothy M. Nolan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Timothy M. Nolan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5109806931"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oligodendrocyte-precursor-cells","display_name":"oligodendrocyte precursor cells","score":0.9909999966621399},{"id":"https://openalex.org/keywords/gabaergic-interneurons","display_name":"GABAergic interneurons","score":0.9399999976158142},{"id":"https://openalex.org/keywords/parvalbumin","display_name":"parvalbumin","score":0.824999988079071},{"id":"https://openalex.org/keywords/somatostatin","display_name":"somatostatin","score":0.9919999837875366},{"id":"https://openalex.org/keywords/gad67","display_name":"GAD67","score":0.8539999723434448},{"id":"https://openalex.org/keywords/gene-expression-profiling","display_name":"gene expression profiling","score":0.6349999904632568},{"id":"https://openalex.org/keywords/neuronal-differentiation","display_name":"neuronal differentiation","score":0.7770000100135803},{"id":"https://openalex.org/keywords/mixed-phenotype","display_name":"mixed phenotype","score":0.40299999713897705}],"concepts":[{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7233110070228577},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5943278074264526},{"id":"https://openalex.org/C2779296341","wikidata":"https://www.wikidata.org/wiki/Q1500732","display_name":"Interneuron","level":3,"score":0.519478440284729},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.40181297063827515},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3612234890460968},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3464449942111969},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.275409996509552},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2706349492073059},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.2583838999271393},{"id":"https://openalex.org/C17077164","wikidata":"https://www.wikidata.org/wiki/Q1185869","display_name":"Inhibitory postsynaptic potential","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:opencommons.uconn.edu:srhonors_theses-1657","doi":null,"is_oa":true,"landing_page_url":"https://opencommons.uconn.edu/srhonors_theses/647","pdf_url":"https://digitalcommons.lib.uconn.edu/cgi/viewcontent.cgi?article=1657&context=srhonors_theses","source":{"id":"https://openalex.org/S4306401180","display_name":"OpenCommons at University of Connecticut (University of Connecticut)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I140172145","host_organization_name":"University of Connecticut","host_organization_lineage":["https://openalex.org/I140172145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Honors Scholar Theses","raw_type":"text"},{"id":"pmh:oai:digitalcommons.lib.uconn.edu:srhonors_theses-1657","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.lib.uconn.edu/srhonors_theses/647","pdf_url":null,"source":{"id":"https://openalex.org/S4377196438","display_name":"OpenCommons - UConn (University of Connecticut)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I140172145","host_organization_name":"University of Connecticut","host_organization_lineage":["https://openalex.org/I140172145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Honors Scholar Theses","raw_type":"text"},{"id":"mag:2999237457","doi":null,"is_oa":false,"landing_page_url":"https://opencommons.uconn.edu/cgi/viewcontent.cgi?article=1657&context=srhonors_theses","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:opencommons.uconn.edu:srhonors_theses-1657","doi":null,"is_oa":true,"landing_page_url":"https://opencommons.uconn.edu/srhonors_theses/647","pdf_url":"https://digitalcommons.lib.uconn.edu/cgi/viewcontent.cgi?article=1657&context=srhonors_theses","source":{"id":"https://openalex.org/S4306401180","display_name":"OpenCommons at University of Connecticut (University of Connecticut)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I140172145","host_organization_name":"University of Connecticut","host_organization_lineage":["https://openalex.org/I140172145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Honors Scholar Theses","raw_type":"text"},"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310017","display_name":"University of Connecticut","ror":"https://ror.org/02der9h97"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2999237457.pdf","grobid_xml":"https://content.openalex.org/works/W2999237457.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1535602073","https://openalex.org/W1604444942","https://openalex.org/W1875096376","https://openalex.org/W1901736351","https://openalex.org/W1970719523","https://openalex.org/W1971242931","https://openalex.org/W1976152949","https://openalex.org/W1988464397","https://openalex.org/W1991360562","https://openalex.org/W1999272226","https://openalex.org/W2006312185","https://openalex.org/W2008183439","https://openalex.org/W2015038624","https://openalex.org/W2017830127","https://openalex.org/W2022284098","https://openalex.org/W2023105628","https://openalex.org/W2031224620","https://openalex.org/W2036567890","https://openalex.org/W2037632846","https://openalex.org/W2045013955","https://openalex.org/W2047804432","https://openalex.org/W2049212652","https://openalex.org/W2051939267","https://openalex.org/W2058915525","https://openalex.org/W2062450961","https://openalex.org/W2079944307","https://openalex.org/W2085424855","https://openalex.org/W2089771991","https://openalex.org/W2090249462","https://openalex.org/W2095490681","https://openalex.org/W2103279601","https://openalex.org/W2107460318","https://openalex.org/W2110622678","https://openalex.org/W2110934330","https://openalex.org/W2111888024","https://openalex.org/W2113964043","https://openalex.org/W2124019758","https://openalex.org/W2131169353","https://openalex.org/W2135995702","https://openalex.org/W2143089517","https://openalex.org/W2199511631","https://openalex.org/W2549724598","https://openalex.org/W2749773064","https://openalex.org/W2768784295","https://openalex.org/W2789083734","https://openalex.org/W2800436528","https://openalex.org/W7095890978"],"related_works":["https://openalex.org/W3128101862","https://openalex.org/W1547964275","https://openalex.org/W7169682868","https://openalex.org/W2012481409","https://openalex.org/W2025107200","https://openalex.org/W2068170814","https://openalex.org/W2745263591","https://openalex.org/W2027064914","https://openalex.org/W2021593652","https://openalex.org/W4286855677"],"abstract_inverted_index":{"Neurons":[0],"are":[1,9,29,48,121,190,227,234],"a":[2,17,71,196,230,245],"post-mitotic":[3],"cell":[4],"population,":[5],"and":[6,24,77,129,139,162,203],"therefore,":[7],"they":[8],"not":[10,220,235],"able":[11],"to":[12,62,84,127,143,200,242],"regenerate":[13],"in":[14,74,182,213],"vivo":[15],"after":[16],"traumatic":[18],"injury.":[19],"Because":[20],"inhibitory":[21],"GABAergic":[22,44,52,75,106,115,193,207],"interneurons":[23,53,116,208],"oligodendrocyte":[25],"precursor":[26],"cells":[27,95,120,151,189,226],"(OPCs)":[28],"derived":[30],"from":[31],"the":[32,159,172,183,201,214],"same":[33],"precursor,":[34],"recent":[35],"studies":[36],"have":[37,55],"focused":[38],"on":[39],"transforming":[40],"these":[41,93,145,188,225],"OPCs":[42],"into":[43,192],"neurons.":[45],"However,":[46],"there":[47],"different":[49,56],"types":[50,205],"of":[51,92,114,149,206],"that":[54,187,224],"electrophysiological":[57],"responses,":[58],"which":[59],"can":[60],"lead":[61],"functional":[63],"differences.":[64],"The":[65],"Nishiyama":[66],"laboratory":[67],"had":[68],"already":[69],"used":[70],"key":[72],"gene":[73,173],"interneuron":[76],"OPC":[78],"differentiation,":[79],"Distal-less":[80],"homeobox":[81],"2":[82],"(Dlx-2),":[83],"transfect":[85],"OPCs;":[86],"early":[87],"electrophysiology":[88],"tests":[89],"showed":[90],"most":[91,217],"transfected":[94,119,150],"behaved":[96,102],"like":[97],"immature":[98],"neurons,":[99],"but":[100],"some":[101],"how":[103],"fast-spiking":[104],"parvalbumin-containing":[105],"neurons":[107],"would.":[108],"To":[109],"further":[110],"clarify":[111],"what":[112],"type":[113],"our":[117],"Dlx-2":[118],"becoming,":[122],"I":[123],"chose":[124],"fourteen":[125],"genes":[126,198,218],"quantify":[128],"compare,":[130],"utilizing":[131],"reverse-transcriptase":[132],"polymerase":[133],"chain":[134],"reactions":[135],"(qRT-PCR).":[136],"After":[137],"designing":[138],"optimizing":[140],"primer":[141],"pairs":[142],"target":[144],"genes,":[146],"five":[147],"replicates":[148],"were":[152,155,209,219],"tested;":[153],"results":[154],"analyzed":[156],"using":[157],"both":[158],"2\u2212\u0394\u0394CT":[160],"method":[161],"by":[163],"evaluating":[164],"raw":[165],"cycle":[166],"thresholds":[167],"for":[168,175],"each":[169],"gene.":[170],"Gad67,":[171],"responsible":[174],"producing":[176],"GABA,":[177],"was":[178],"significantly":[179,210,221],"more":[180,211],"expressed":[181,212],"Dlx-2-transfected":[184,215],"samples,":[185,216],"showing":[186],"differentiating":[191],"interneurons.":[194],"Although":[195],"few":[197],"linked":[199],"parvalbumin-":[202],"somatostatin-containing":[204],"changed,":[222],"suggesting":[223],"either":[228],"expressing":[229],"mixed":[231],"phenotype":[232],"or":[233],"developed":[236],"enough":[237],"at":[238],"this":[239],"time":[240],"point":[241],"clearly":[243],"show":[244],"subtype.":[246]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2020-01-23T00:00:00"}
