{"id":"https://openalex.org/W2999199337","doi":"https://doi.org/10.7171/jbt.20-3101-002","title":"SPRI Beads-based Size Selection in the Range of 2-10kb","display_name":"SPRI Beads-based Size Selection in the Range of 2-10kb","publication_year":2020,"publication_date":"2020-01-10","ids":{"openalex":"https://openalex.org/W2999199337","doi":"https://doi.org/10.7171/jbt.20-3101-002","mag":"2999199337","pmid":"https://pubmed.ncbi.nlm.nih.gov/31920466","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6944320"},"language":"en","primary_location":{"id":"doi:10.7171/jbt.20-3101-002","doi":"https://doi.org/10.7171/jbt.20-3101-002","is_oa":true,"landing_page_url":"https://doi.org/10.7171/jbt.20-3101-002","pdf_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944320/pdf/jbt.31-7.pdf","source":{"id":"https://openalex.org/S2755344187","display_name":"Journal of Biomolecular Techniques JBT","issn_l":"1524-0215","issn":["1524-0215","1943-4731"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4360969280","host_organization_name":"Association of Biomolecular Resource Facilities","host_organization_lineage":["https://openalex.org/P4360969280"],"host_organization_lineage_names":["Association of Biomolecular Resource Facilities"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biomolecular Techniques : JBT","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944320/pdf/jbt.31-7.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078872998","display_name":"Alexei Stortchevoi","orcid":"https://orcid.org/0000-0001-9916-4344","observed_orcids":["https://orcid.org/0000-0001-9916-4344"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexei Stortchevoi","raw_affiliation_strings":["Department of Biology, BioMicro Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","BioMicro Center, Massachusetts Institute of Technology, 77 Mas-sachusetts Ave. 68-304D, Cambridge, MA 02139, USA","Department of Biology,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, BioMicro Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"BioMicro Center, Massachusetts Institute of Technology, 77 Mas-sachusetts Ave. 68-304D, Cambridge, MA 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Biology,","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041567598","display_name":"Noelani Kamelamela","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Noelani Kamelamela","raw_affiliation_strings":["Department of Biology, BioMicro Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","BioMicro Center, Massachusetts Institute of Technology, 77 Mas-sachusetts Ave. 68-304D, Cambridge, MA 02139, USA","Department of Biology,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, BioMicro Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"BioMicro Center, Massachusetts Institute of Technology, 77 Mas-sachusetts Ave. 68-304D, Cambridge, MA 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Biology,","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026357113","display_name":"Stuart S. Levine","orcid":"https://orcid.org/0000-0001-7363-562X","observed_orcids":["https://orcid.org/0000-0001-7363-562X"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Stuart S. Levine","raw_affiliation_strings":["Department of Biology, BioMicro Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","BioMicro Center, Massachusetts Institute of Technology, 77 Mas-sachusetts Ave. 68-304D, Cambridge, MA 02139, USA","Department of Biology,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, BioMicro Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"BioMicro Center, Massachusetts Institute of Technology, 77 Mas-sachusetts Ave. 68-304D, Cambridge, MA 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Biology,","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5026357113"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":null,"apc_paid":null,"fwci":1.6822,"has_fulltext":true,"cited_by_count":45,"citation_normalized_percentile":{"value":0.8385804,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"31","issue":"1","first_page":"7","last_page":"10"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.7738999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.7738999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.06109999865293503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.029400000348687172,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nacl-concentration","display_name":"NaCl concentration","score":0.7450000047683716},{"id":"https://openalex.org/keywords/polyethylene-glycol","display_name":"polyethylene glycol","score":0.8420000076293945},{"id":"https://openalex.org/keywords/high-molecular-weight-dna","display_name":"high molecular weight DNA","score":0.593999981880188}],"concepts":[{"id":"https://openalex.org/C2776964284","wikidata":"https://www.wikidata.org/wiki/Q410083","display_name":"Polyethylene glycol","level":2,"score":0.8443886041641235},{"id":"https://openalex.org/C54400483","wikidata":"https://www.wikidata.org/wiki/Q1793202","display_name":"PEG ratio","level":2,"score":0.7419554591178894},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.6311264038085938},{"id":"https://openalex.org/C105341887","wikidata":"https://www.wikidata.org/wiki/Q1307987","display_name":"Suspension (topology)","level":3,"score":0.5830994844436646},{"id":"https://openalex.org/C109386097","wikidata":"https://www.wikidata.org/wiki/Q1053956","display_name":"Bead","level":2,"score":0.5508723258972168},{"id":"https://openalex.org/C2993110782","wikidata":"https://www.wikidata.org/wiki/Q3870166","display_name":"Magnetic bead","level":2,"score":0.4743346571922302},{"id":"https://openalex.org/C145018004","wikidata":"https://www.wikidata.org/wiki/Q4985944","display_name":"Buffer (optical fiber)","level":2,"score":0.46651187539100647},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.40828830003738403},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4050554633140564},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3398905396461487},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.32368484139442444},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1095389723777771},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.07763826847076416},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0711660087108612},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.06974291801452637},{"id":"https://openalex.org/C202444582","wikidata":"https://www.wikidata.org/wiki/Q837863","display_name":"Pure mathematics","level":1,"score":0.0},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C5961521","wikidata":"https://www.wikidata.org/wiki/Q746083","display_name":"Homotopy","level":2,"score":0.0},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0},{"id":"https://openalex.org/C10138342","wikidata":"https://www.wikidata.org/wiki/Q43015","display_name":"Finance","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002021","descriptor_name":"Buffers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002412","descriptor_name":"Cations","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011092","descriptor_name":"Polyethylene Glycols","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D053938","descriptor_name":"DNA Fragmentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.7171/jbt.20-3101-002","doi":"https://doi.org/10.7171/jbt.20-3101-002","is_oa":true,"landing_page_url":"https://doi.org/10.7171/jbt.20-3101-002","pdf_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944320/pdf/jbt.31-7.pdf","source":{"id":"https://openalex.org/S2755344187","display_name":"Journal of Biomolecular Techniques JBT","issn_l":"1524-0215","issn":["1524-0215","1943-4731"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4360969280","host_organization_name":"Association of Biomolecular Resource Facilities","host_organization_lineage":["https://openalex.org/P4360969280"],"host_organization_lineage_names":["Association of Biomolecular Resource Facilities"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biomolecular Techniques : JBT","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6944320","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6944320","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biomol Tech","raw_type":"Text"},{"id":"pmid:31920466","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31920466","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biomolecular techniques : JBT","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.7171/jbt.20-3101-002","doi":"https://doi.org/10.7171/jbt.20-3101-002","is_oa":true,"landing_page_url":"https://doi.org/10.7171/jbt.20-3101-002","pdf_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944320/pdf/jbt.31-7.pdf","source":{"id":"https://openalex.org/S2755344187","display_name":"Journal of Biomolecular Techniques JBT","issn_l":"1524-0215","issn":["1524-0215","1943-4731"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4360969280","host_organization_name":"Association of Biomolecular Resource Facilities","host_organization_lineage":["https://openalex.org/P4360969280"],"host_organization_lineage_names":["Association of Biomolecular Resource Facilities"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biomolecular Techniques : JBT","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1148400999","display_name":null,"funder_award_id":"ES-002109","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G3909382221","display_name":"MIT Center for Environmental Health Sciences","funder_award_id":"P30ES002109","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G4774430914","display_name":null,"funder_award_id":"CA14051","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000300833","display_name":null,"funder_award_id":"ES002109","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000891960","display_name":"Cancer Center Support (Core) Grant","funder_award_id":"P30CA014051","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2999199337.pdf","grobid_xml":"https://content.openalex.org/works/W2999199337.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4396232363","https://openalex.org/W2995985818","https://openalex.org/W4409110807","https://openalex.org/W4405496359","https://openalex.org/W4238983547","https://openalex.org/W4327679993","https://openalex.org/W4392202119","https://openalex.org/W4391237665","https://openalex.org/W3096984264","https://openalex.org/W3011502015"],"abstract_inverted_index":{"Application":[0],"of":[1,18,21,30,38,50,63,73,88,98,114],"solid-phase":[2],"reversible":[3],"immobilization":[4],"(SPRI)":[5],"beads":[6,23,75,100],"for":[7,110],"size":[8,33,47],"selection":[9,48],"in":[10,16,65],"molecular":[11],"biology":[12],"should":[13],"be":[14,53],"expanded,":[15],"light":[17],"the":[19,22,45,61,66,86,96],"property":[20],"to":[24,26,55],"accommodate":[25],"high":[27,104],"MW":[28,71],"intervals":[29],"DNA":[31],"fragment":[32],"selection,":[34],"depending":[35],"on":[36],"composition":[37],"bead-suspension":[39],"buffer.":[40,69],"Here":[41],"we":[42],"show":[43],"how":[44],"conventional":[46],"interval":[49],"150-800":[51],"bp":[52],"shifted":[54],"1.5-7":[56],"Kbp":[57,107],"with":[58,81],"by":[59],"adjusting":[60],"concentration":[62,87],"NaCl":[64,79],"stock":[67],"suspension":[68],"The":[70],"capacity":[72],"SPRI":[74,99,116],"also":[76],"change":[77],"when":[78,85],"replaced":[80],"other":[82],"cations":[83],"and":[84],"polyethylene":[89],"glycol":[90],"(PEG)":[91],"8000":[92],"is":[93],"decreased.":[94],"Testing":[95],"limits":[97],"revealed":[101],"cuts":[102],"as":[103,105],"10":[106],"are":[108],"possible":[109],"some":[111],"salt/PEG":[112],"combinations":[113],"modified":[115],"beads-suspension":[117],"buffers.":[118]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":10},{"year":2023,"cited_by_count":13},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2020-01-23T00:00:00"}