{"id":"https://openalex.org/W2997655971","doi":"https://doi.org/10.1136/heartjnl-2019-bcs.210","title":"BS49 Human embryonic stem cell derived cardiomyocytes express functional receptors for the cardiovascular peptide apelin","display_name":"BS49 Human embryonic stem cell derived cardiomyocytes express functional receptors for the cardiovascular peptide apelin","publication_year":2019,"publication_date":"2019-05-01","ids":{"openalex":"https://openalex.org/W2997655971","doi":"https://doi.org/10.1136/heartjnl-2019-bcs.210","mag":"2997655971"},"language":"en","primary_location":{"id":"doi:10.1136/heartjnl-2019-bcs.210","doi":"https://doi.org/10.1136/heartjnl-2019-bcs.210","is_oa":true,"landing_page_url":"https://doi.org/10.1136/heartjnl-2019-bcs.210","pdf_url":"https://heart.bmj.com/content/heartjnl/105/Suppl_6/A171.full.pdf","source":{"id":"https://openalex.org/S191565391","display_name":"Heart","issn_l":"1355-6037","issn":["1355-6037","1468-201X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Basic Science","raw_type":"proceedings-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://heart.bmj.com/content/heartjnl/105/Suppl_6/A171.full.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023111250","display_name":"Robyn G. C. Macrae","orcid":"https://orcid.org/0000-0002-8129-7242","observed_orcids":["https://orcid.org/0000-0002-8129-7242"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Robyn Macrae","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042832631","display_name":"William George Bernard","orcid":"https://orcid.org/0000-0002-2622-5115","observed_orcids":["https://orcid.org/0000-0002-2622-5115"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"William G. Bernard","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010203060","display_name":"Rhoda E. Kuc","orcid":"https://orcid.org/0000-0003-4673-1602","observed_orcids":["https://orcid.org/0000-0003-4673-1602"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Rhoda E. Kuc","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044067769","display_name":"Maria Colzani","orcid":"https://orcid.org/0000-0002-3659-5902","observed_orcids":["https://orcid.org/0000-0002-3659-5902"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Maria T. Colzani","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057114115","display_name":"Thomas Williams","orcid":"https://orcid.org/0000-0002-1051-0595","observed_orcids":["https://orcid.org/0000-0002-1051-0595"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Thomas Williams","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005004865","display_name":"Duuamene Nyimanu","orcid":"https://orcid.org/0000-0002-6212-9518","observed_orcids":["https://orcid.org/0000-0002-6212-9518"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Duuamene Nyimanu","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019791662","display_name":"Janet J. Maguire","orcid":"https://orcid.org/0000-0002-9254-7040","observed_orcids":["https://orcid.org/0000-0002-9254-7040"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Janet Maguire","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048692866","display_name":"Sanjay Sinha","orcid":"https://orcid.org/0000-0001-5900-1209","observed_orcids":["https://orcid.org/0000-0001-5900-1209"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Sanjay Sinha","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060689377","display_name":"Anthony P. Davenport","orcid":"https://orcid.org/0000-0002-2096-3117","observed_orcids":["https://orcid.org/0000-0002-2096-3117"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Anthony P. Davenport","raw_affiliation_strings":["University of Cambridge"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Cambridge","institution_ids":["https://openalex.org/I241749"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I241749"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"A171","last_page":"A172"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13505","display_name":"Apelin-related biomedical research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T13505","display_name":"Apelin-related biomedical research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10814","display_name":"Neuropeptides and Animal Physiology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/apelin-receptor","display_name":"apelin receptor","score":0.9739999771118164},{"id":"https://openalex.org/keywords/human-embryonic-stem-cell-derived-cardiomyocytes","display_name":"human embryonic stem cell-derived cardiomyocytes","score":0.9110000133514404},{"id":"https://openalex.org/keywords/apelin","display_name":"apelin","score":0.7160000205039978},{"id":"https://openalex.org/keywords/elabela","display_name":"Elabela","score":0.8149999976158142},{"id":"https://openalex.org/keywords/receptor-density","display_name":"receptor density","score":0.6320000290870667},{"id":"https://openalex.org/keywords/receptor-binding-affinity","display_name":"receptor binding affinity","score":0.7639999985694885},{"id":"https://openalex.org/keywords/apelin-13","display_name":"apelin-13","score":0.5680000185966492},{"id":"https://openalex.org/keywords/cardiomyocyte-differentiation","display_name":"cardiomyocyte differentiation","score":0.628000020980835},{"id":"https://openalex.org/keywords/pulmonary-arterial-hypertension","display_name":"pulmonary arterial hypertension","score":0.7710000276565552},{"id":"https://openalex.org/keywords/h9-cell-line","display_name":"H9 cell line","score":0.6050000190734863},{"id":"https://openalex.org/keywords/cardiac-troponin-t","display_name":"cardiac troponin T","score":0.8410000205039978}],"concepts":[{"id":"https://openalex.org/C2779546753","wikidata":"https://www.wikidata.org/wiki/Q2386988","display_name":"Apelin","level":3,"score":0.9517676830291748},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.7628248929977417},{"id":"https://openalex.org/C67847695","wikidata":"https://www.wikidata.org/wiki/Q626478","display_name":"Radioligand","level":3,"score":0.5502970218658447},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.525423526763916},{"id":"https://openalex.org/C35866371","wikidata":"https://www.wikidata.org/wiki/Q6005502","display_name":"Immunocytochemistry","level":2,"score":0.5124309659004211},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.48311519622802734},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.44723325967788696},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4182341694831848},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.35812634229660034},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.30116450786590576},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16134822368621826},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10744273662567139}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1136/heartjnl-2019-bcs.210","doi":"https://doi.org/10.1136/heartjnl-2019-bcs.210","is_oa":true,"landing_page_url":"https://doi.org/10.1136/heartjnl-2019-bcs.210","pdf_url":"https://heart.bmj.com/content/heartjnl/105/Suppl_6/A171.full.pdf","source":{"id":"https://openalex.org/S191565391","display_name":"Heart","issn_l":"1355-6037","issn":["1355-6037","1468-201X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Basic Science","raw_type":"proceedings-article"}],"best_oa_location":{"id":"doi:10.1136/heartjnl-2019-bcs.210","doi":"https://doi.org/10.1136/heartjnl-2019-bcs.210","is_oa":true,"landing_page_url":"https://doi.org/10.1136/heartjnl-2019-bcs.210","pdf_url":"https://heart.bmj.com/content/heartjnl/105/Suppl_6/A171.full.pdf","source":{"id":"https://openalex.org/S191565391","display_name":"Heart","issn_l":"1355-6037","issn":["1355-6037","1468-201X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Basic Science","raw_type":"proceedings-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.98580002784729,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2997655971.pdf","grobid_xml":"https://content.openalex.org/works/W2997655971.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W6962775649","https://openalex.org/W3173069774","https://openalex.org/W4226191556","https://openalex.org/W2030433820","https://openalex.org/W2348492516","https://openalex.org/W2883758390","https://openalex.org/W1872062849","https://openalex.org/W3013590749","https://openalex.org/W1585260982","https://openalex.org/W2090353883"],"abstract_inverted_index":{"Introduction":[0],"The":[1],"apelin":[2,22,66,92,161,235,246,270,329],"receptor":[3,67,72,93,136,148,162,186,205,271,281,330],"is":[4],"expressed":[5],"throughout":[6],"the":[7,40,160,167,251,287,309,314],"cardiovascular":[8],"system,":[9],"including":[10,255,298],"in":[11,44,163,189,218,236,248,286,313],"cardiomyocytes.":[12,105,192],"Receptor":[13],"activation":[14],"by":[15,170,326],"either":[16],"of":[17,135,159,337],"its":[18],"endogenous":[19],"peptide":[20],"ligands,":[21],"or":[23,149],"Elabela,":[24],"has":[25],"a":[26,84,230],"positive":[27,244],"inotropic":[28],"effect":[29],"and":[30,53,69,114,124,139,196,204,318],"promotes":[31],"vasodilatation.":[32],"Human":[33],"embryonic":[34],"stem":[35],"cell":[36,78,151],"(hESC)-derived":[37],"cells":[38,177],"have":[39,184,283],"potential":[41,54],"for":[42,87,234,245],"use":[43],"translational":[45],"research":[46],"to":[47,60,70,74,100,103,116,132,175,208,215,226,250,276,306,321],"investigate":[48],"cellular":[49],"signalling,":[50],"disease":[51],"pathogenesis":[52],"novel":[55],"treatments.":[56],"Our":[57,302],"aim":[58,305],"was":[59,122,143,194,224],"determine":[61],"if":[62,76],"hESC-derived":[63,164,191,241,267,316,323],"cardiomyocytes":[64,165,242,268,317],"express":[65,269],"protein":[68,187,272],"quantify":[71],"density":[73,140,206],"ascertain":[75],"this":[77],"type":[79],"can":[80],"be":[81,209],"used":[82],"as":[83],"phenotypic":[85,324],"model":[86],"human":[88,219,278],"diseases":[89],"associated":[90,294],"with":[91,199,229,295],"mutations.":[94],"Methods":[95],"H9":[96],"hESCs":[97],"were":[98,110],"cultured":[99],"induce":[101],"differentiation":[102],"beating":[104,190,240,315],"Saturation":[106],"radioligand":[107,181],"binding":[108,182,232],"experiments":[109,304],"performed":[111],"using":[112,127,146],"[Glp65,Nle75,Tyr77][125I]apelin-13":[113],"[Pyr1]apelin-13":[115],"define":[117],"non-specific":[118],"binding.":[119],"Bound":[120],"radioactivity":[121],"counted":[123],"data":[125,263],"analysed":[126],"iterative":[128],"curve":[129],"fitting":[130],"programs":[131],"obtain":[133],"values":[134],"affinity":[137,201],"(KD)":[138],"(BMAX).":[141],"Immunocytochemistry":[142],"carried":[144],"out":[145],"anti-apelin":[147],"anti-cardiac":[150],"marker":[152],"antibodies.":[153],"Results":[154],"Previous":[155],"work":[156],"demonstrated":[157],"expression":[158,188],"at":[166,172,273],"gene":[168,334],"level":[169],"qRT-PCR":[171],"similar":[173,274],"levels":[174,275],"adult":[176,220,277],"(figure":[178,212,259],"1A).":[179],"Here,":[180],"studies":[183],"confirmed":[185],"Binding":[193],"saturable":[195],"[125I]apelin-13":[197],"bound":[198],"sub-nanomolar":[200],"(0.12":[202],"nM)":[203],"found":[207,217],"21":[210],"fmol/mg":[211],"1B),":[213],"comparable":[214],"that":[216,266,292],"heart.":[221,279],"Hill":[222],"slope":[223],"close":[225],"1":[227],"consistent":[228],"single":[231],"site":[233],"these":[237],"cells.":[238],"Furthermore,":[239],"stained":[243],"receptor,":[247],"addition":[249],"standard":[252],"cardiac":[253,256],"markers":[254],"troponin":[257],"T":[258],"2).":[260],"Conclusion":[261],"These":[262],"importantly":[264],"confirm":[265],"Apelin":[280],"mutations":[282,331],"been":[284],"identified":[285],"100,000":[288],"Genomes":[289],"Bridge":[290],"Project":[291],"are":[293],"rare":[296],"diseases,":[297],"pulmonary":[299],"arterial":[300],"hypertension.":[301],"ongoing":[303],"pharmacologically":[307],"characterise":[308],"apelinergic":[310],"signalling":[311],"pathway":[312],"we":[319],"propose":[320],"generate":[322],"models":[325],"introducing":[327],"selected":[328],"via":[332],"CRISPR/Cas-9":[333],"editing.":[335],"Conflict":[336],"interest":[338],"None":[339]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2020-01-10T00:00:00"}