{"id":"https://openalex.org/W2997513063","doi":null,"title":"The interaction between MsrA and Csn5/Jab1","display_name":"The interaction between MsrA and Csn5/Jab1","publication_year":2019,"publication_date":"2019-08-31","ids":{"openalex":"https://openalex.org/W2997513063","mag":"2997513063"},"language":"en","primary_location":{"id":"pmh:oai:kuscholarworks.ku.edu:1808/29844","doi":null,"is_oa":false,"landing_page_url":"https://kuscholarworks.ku.edu/bitstream/1808/29844/1/Jiang_ku_0099M_16665_DATA_1.pdf","pdf_url":null,"source":{"id":"https://openalex.org/S4306400421","display_name":"KU ScholarWorks (The University of Kansas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I146416000","host_organization_name":"University of Kansas","host_organization_lineage":["https://openalex.org/I146416000"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087076866","display_name":"Beichen Jiang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Beichen Jiang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5087076866"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.6122999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.6122999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.3822999894618988,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10445","display_name":"Antioxidant Activity and Oxidative Stress","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2704","display_name":"Biochemistry (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cop9-signalosome","display_name":"COP9 signalosome","score":0.9959999918937683},{"id":"https://openalex.org/keywords/deneddylation","display_name":"deneddylation","score":0.8550000190734863},{"id":"https://openalex.org/keywords/neddylation","display_name":"neddylation","score":0.8600000143051147},{"id":"https://openalex.org/keywords/nedd8","display_name":"NEDD8","score":0.8190000057220459},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.6779999732971191},{"id":"https://openalex.org/keywords/methionine-sulfoxide","display_name":"methionine sulfoxide","score":0.8510000109672546},{"id":"https://openalex.org/keywords/ubiquitin-like-modification","display_name":"ubiquitin-like modification","score":0.5820000171661377},{"id":"https://openalex.org/keywords/cul1","display_name":"CUL1","score":0.6930000185966492},{"id":"https://openalex.org/keywords/scf-ubiquitin-ligase","display_name":"SCF ubiquitin ligase","score":0.7720000147819519},{"id":"https://openalex.org/keywords/brain","display_name":"brain","score":0.41499999165534973},{"id":"https://openalex.org/keywords/mouse-brain","display_name":"mouse brain","score":0.41499999165534973},{"id":"https://openalex.org/keywords/yeast-two-hybrid-screen","display_name":"yeast two-hybrid screen","score":0.6029999852180481},{"id":"https://openalex.org/keywords/hydrogen-peroxide","display_name":"hydrogen peroxide","score":0.6079999804496765}],"concepts":[{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.37956926226615906}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:kuscholarworks.ku.edu:1808/29844","doi":null,"is_oa":false,"landing_page_url":"https://kuscholarworks.ku.edu/bitstream/1808/29844/1/Jiang_ku_0099M_16665_DATA_1.pdf","pdf_url":null,"source":{"id":"https://openalex.org/S4306400421","display_name":"KU ScholarWorks (The University of Kansas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I146416000","host_organization_name":"University of Kansas","host_organization_lineage":["https://openalex.org/I146416000"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:2997513063","doi":null,"is_oa":false,"landing_page_url":"https://kuscholarworks.ku.edu/handle/1808/29844","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4043000042438507}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W1541556706","https://openalex.org/W1553874478","https://openalex.org/W1881534971","https://openalex.org/W1964909962","https://openalex.org/W1968411230","https://openalex.org/W1973440823","https://openalex.org/W1981126988","https://openalex.org/W1986128576","https://openalex.org/W2001501814","https://openalex.org/W2002630364","https://openalex.org/W2004110947","https://openalex.org/W2011667964","https://openalex.org/W2012164150","https://openalex.org/W2021846401","https://openalex.org/W2024988493","https://openalex.org/W2044823713","https://openalex.org/W2053763318","https://openalex.org/W2066753129","https://openalex.org/W2087292928","https://openalex.org/W2089626228","https://openalex.org/W2091583229","https://openalex.org/W2098980778","https://openalex.org/W2116777384","https://openalex.org/W2119785969","https://openalex.org/W2121477827","https://openalex.org/W2137382956","https://openalex.org/W2140219059","https://openalex.org/W2164201106","https://openalex.org/W2329012907","https://openalex.org/W2513505388","https://openalex.org/W2534266625","https://openalex.org/W2809613254"],"related_works":["https://openalex.org/W3027720852","https://openalex.org/W2751525612","https://openalex.org/W2886329189","https://openalex.org/W2769825522","https://openalex.org/W2034104584","https://openalex.org/W2011667964","https://openalex.org/W2087767556","https://openalex.org/W2593840797","https://openalex.org/W2156537651","https://openalex.org/W2088938488"],"abstract_inverted_index":{"Oxidative":[0],"stress":[1,335],"can":[2],"cause":[3],"toxic":[4],"outcomes":[5],"to":[6,82,119,362,428,497,528],"cells/organisms\u2019":[7],"survival.":[8],"To":[9,64,278],"counter":[10],"this":[11,274,498],"toxicity,":[12],"multiple":[13],"cellular":[14],"mechanisms":[15],"were":[16,249,303,327],"evolved.":[17],"Among":[18],"them":[19],"is":[20,126,461],"the":[21,54,67,94,131,137,150,157,161,174,183,218,227,243,250,254,281,318,347,402,409,414,420,440,447,464,490,505,514,529,550,555,561],"methionine":[22],"sulfoxide":[23,28],"reductase":[24,29],"(Msr)":[25],"system.":[26],"Methionine":[27],"A":[30,224],"(MsrA)":[31],"plays":[32,434],"a":[33,48,76,99,115,153,359,473],"key":[34],"role":[35,50,68,437,449,531],"in":[36,53,71,89,196,208,226,235,247,252,288,346,370,381,408,438,452,457,510,534,538,553,560],"protecting":[37],"cells":[38,559],"from":[39,145],"oxidative":[40,334],"stress.":[41],"In":[42,418],"our":[43,430],"previous":[44],"studies":[45],"we":[46,189,290,373,445],"discovered":[47],"novel":[49],"of":[51,56,62,69,74,93,130,139,160,182,211,229,238,245,273,298,315,320,341,344,349,383,385,394,450,454,463,477,489,516,532],"MsrA":[52,70,88,192,212,239,257,264,287,305,308,388,410,433,451,533,551],"regulation":[55],"ubiquitin":[57,132],"(Ub)":[58],"and":[59,169,177,201,256,286,307,336,356,387,425,557],"Ub-like":[60,142],"modification":[61,73],"proteins.":[63],"further":[65,279,546],"understand":[66],"posttranslational":[72,491],"proteins,":[75],"yeast-2-hybrid":[77],"(Y2H)":[78],"screening":[79],"was":[80,98,206,232,405,508,518],"performed":[81],"screen":[83],"for":[84,87,167],"potential":[85,448],"substrates":[86,97,166],"brain.":[90],"Accordingly,":[91,484],"one":[92,462,488],"major":[95],"identified":[96],"protein":[100,143,275],"denoted":[101],"as":[102,110,392],"COP9":[103,122],"signalosome":[104,123],"subunit":[105],"5":[106],"(CSN5,":[107],"also":[108,233],"known":[109,165],"Jab1":[111],"or":[112,323],"COPS5),":[113],"containing":[114],"unique":[116],"binding":[117],"site":[118],"MsrA.":[120,299],"The":[121,164,329,397],"complex":[124],"(CSN)":[125],"an":[127,141,435],"essential":[128],"regulator":[129],"conjugation":[133],"pathway":[134],"by":[135,548],"mediating":[136],"removal":[138],"Nedd8,":[140],"modifier,":[144],"proteins":[146,493],"(deneddylation":[147],"process).":[148],"Importantly,":[149],"Csn5/Jab1":[151,195,246,267,325,337,345,441,485,517,536],"contains":[152],"domain":[154],"that":[155,191,263,332,401,432,494,504],"provides":[156],"catalytic":[158],"center":[159],"CSN":[162],"complex.":[163],"neddylation":[168,284,455,478,506],"deneddylation":[170,268,342,379,403,442],"(by":[171],"CSN)":[172],"are":[173],"cullin":[175],"subunits":[176],"their":[178],"homologues":[179],"(e.g.":[180],"Cul-1":[181,231],"SCF-type":[184],"E3":[185],"ligase":[186],"complexes).":[187],"Furthermore,":[188],"showed":[190,331,358,400],"interacts":[193],"with":[194,217,294,413,480,521],"mouse":[197],"brain":[198,209,248,416,458,467,482,523],"following":[199],"immunoprecipitations":[200],"pull-down":[202],"experiments.":[203],"This":[204,469,525,542],"interaction":[205],"compromised":[207],"extracts":[210,382],"knockout":[213],"(MsrA":[214],"KO)":[215],"compared":[216,412,479,520],"parent":[219],"wild":[220],"type":[221,471],"(WT)":[222],"brains.":[223],"decrease":[225],"levels":[228,244,297,314,456],"neddylated":[230],"observed":[234],"liver":[236],"extract":[237],"KO":[240,258,306,389,411],"mouse,":[241],"while":[242,351,513],"same":[251],"both":[253,333,554],"WT":[255,355,386,415],"strains.":[259,312],"These":[260,300],"data":[261,330,399,422],"suggested":[262],"positively":[265],"regulates":[266],"activity,":[269],"presumably":[270],"via":[271],"reduction":[272],"MetO":[276],"residue/s.":[277],"study":[280],"relationship":[282],"between":[283],"level":[285,476,507,515],"vivo,":[289],"used":[291,374],"yeast":[292,301,311,316],"strains":[293,302,353,391],"various":[295],"expression":[296],"WT,":[304],"overexpressed":[309],"(OE)":[310],"Neddylation":[313],"under":[317],"condition":[319],"hydrogen":[321],"peroxide":[322],"human":[324],"inhibitor":[326,338,366],"investigated.":[328],"caused":[339],"inhibition":[340],"activity":[343,380,404,537],"absence":[348],"MsrA,":[350],"MsrA-containing":[352],"(i.e.":[354],"OE)":[357],"strong":[360],"ability":[361],"protect":[363],"against":[364],"H2O2/Csn5/Jab1":[365],"induced":[367],"neddylation.":[368],"For":[369],"vitro":[371],"assays,":[372],"artificial":[375],"Nedd8":[376],"conjugates":[377],"monitoring":[378],"brains":[384],"mice":[390],"function":[393],"incubation":[395],"time.":[396],"acquired":[398],"dramatically":[406],"reduced":[407,509],"extracts.":[417],"conclusion,":[419],"presented":[421],"provide":[423],"direct":[424],"indirect":[426],"evidence":[427],"support":[429],"hypothesis":[431],"important":[436],"maintaining":[439],"activity.":[443],"Lastly,":[444],"investigated":[446,547],"regulating":[453],"cancer.":[459],"Glioblastoma":[460],"most":[465],"aggressive":[466],"cancers.":[468],"cancer":[470,540],"demonstrates":[472],"relative":[474],"low":[475],"normal":[481,522,556],"cells.":[483,524,541],"could":[486],"be":[487,545],"regulated":[492],"may":[495],"contribute":[496],"phenomenon.":[499],"We":[500],"have":[501],"indeed":[502],"found":[503],"glioblastoma":[511,558],"cells,":[512],"unchanged":[519],"observation":[526],"pointed":[527],"possible":[530],"enhancing":[535],"these":[539],"possibility":[543],"will":[544],"comparing":[549],"activities":[552],"nearest":[562],"future.":[563]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2020-01-10T00:00:00"}
