{"id":"https://openalex.org/W2997326360","doi":"https://doi.org/10.1002/cbin.11304","title":"Fructose\u20101,6\u2010bisphosphatase degradation in the methylotrophic yeast Komagataella phaffii occurs in autophagy pathway","display_name":"Fructose\u20101,6\u2010bisphosphatase degradation in the methylotrophic yeast Komagataella phaffii occurs in autophagy pathway","publication_year":2020,"publication_date":"2020-01-06","ids":{"openalex":"https://openalex.org/W2997326360","doi":"https://doi.org/10.1002/cbin.11304","mag":"2997326360","pmid":"https://pubmed.ncbi.nlm.nih.gov/31903651"},"language":"en","primary_location":{"id":"doi:10.1002/cbin.11304","doi":"https://doi.org/10.1002/cbin.11304","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbin.11304","pdf_url":null,"source":{"id":"https://openalex.org/S7325069","display_name":"Cell Biology International","issn_l":"1065-6995","issn":["1065-6995","1095-8355"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Biology International","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5008456782","display_name":"Olena V. Dmytruk","orcid":"https://orcid.org/0000-0002-9703-7250","observed_orcids":["https://orcid.org/0000-0002-9703-7250"]},"institutions":[{"id":"https://openalex.org/I149851306","display_name":"National Academy of Sciences of Ukraine","ror":"https://ror.org/00je4t102","country_code":"UA","type":"government","lineage":["https://openalex.org/I149851306"]},{"id":"https://openalex.org/I4210155364","display_name":"Institute of Cell Biology","ror":"https://ror.org/04fg7fb49","country_code":"UA","type":"facility","lineage":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}],"countries":["UA"],"is_corresponding":false,"raw_author_name":"Olena Dmytruk","raw_affiliation_strings":["Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine","institution_ids":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034719713","display_name":"N. V. Bulbotka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149851306","display_name":"National Academy of Sciences of Ukraine","ror":"https://ror.org/00je4t102","country_code":"UA","type":"government","lineage":["https://openalex.org/I149851306"]},{"id":"https://openalex.org/I4210155364","display_name":"Institute of Cell Biology","ror":"https://ror.org/04fg7fb49","country_code":"UA","type":"facility","lineage":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}],"countries":["UA"],"is_corresponding":false,"raw_author_name":"Nina Bulbotka","raw_affiliation_strings":["Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine","institution_ids":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009971621","display_name":"Anastasiya Z. Zazulya","orcid":"https://orcid.org/0000-0003-3877-2500","observed_orcids":["https://orcid.org/0000-0003-3877-2500"]},"institutions":[{"id":"https://openalex.org/I149851306","display_name":"National Academy of Sciences of Ukraine","ror":"https://ror.org/00je4t102","country_code":"UA","type":"government","lineage":["https://openalex.org/I149851306"]},{"id":"https://openalex.org/I4210155364","display_name":"Institute of Cell Biology","ror":"https://ror.org/04fg7fb49","country_code":"UA","type":"facility","lineage":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}],"countries":["UA"],"is_corresponding":false,"raw_author_name":"Anastasya Zazulya","raw_affiliation_strings":["Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine","institution_ids":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059770636","display_name":"Marta V. Semkiv","orcid":"https://orcid.org/0000-0002-2780-7096","observed_orcids":["https://orcid.org/0000-0002-2780-7096"]},"institutions":[{"id":"https://openalex.org/I149851306","display_name":"National Academy of Sciences of Ukraine","ror":"https://ror.org/00je4t102","country_code":"UA","type":"government","lineage":["https://openalex.org/I149851306"]},{"id":"https://openalex.org/I4210155364","display_name":"Institute of Cell Biology","ror":"https://ror.org/04fg7fb49","country_code":"UA","type":"facility","lineage":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}],"countries":["UA"],"is_corresponding":false,"raw_author_name":"Marta Semkiv","raw_affiliation_strings":["Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine","institution_ids":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080331561","display_name":"Kostyantyn V. Dmytruk","orcid":"https://orcid.org/0000-0003-3661-8913","observed_orcids":["https://orcid.org/0000-0003-3661-8913"]},"institutions":[{"id":"https://openalex.org/I149851306","display_name":"National Academy of Sciences of Ukraine","ror":"https://ror.org/00je4t102","country_code":"UA","type":"government","lineage":["https://openalex.org/I149851306"]},{"id":"https://openalex.org/I4210155364","display_name":"Institute of Cell Biology","ror":"https://ror.org/04fg7fb49","country_code":"UA","type":"facility","lineage":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}],"countries":["UA"],"is_corresponding":false,"raw_author_name":"Kostyantyn Dmytruk","raw_affiliation_strings":["Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine","institution_ids":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048817862","display_name":"Andriy \u0410. Sibirny","orcid":"https://orcid.org/0000-0001-8579-1566","observed_orcids":["https://orcid.org/0000-0001-8579-1566"]},"institutions":[{"id":"https://openalex.org/I149851306","display_name":"National Academy of Sciences of Ukraine","ror":"https://ror.org/00je4t102","country_code":"UA","type":"government","lineage":["https://openalex.org/I149851306"]},{"id":"https://openalex.org/I4210155364","display_name":"Institute of Cell Biology","ror":"https://ror.org/04fg7fb49","country_code":"UA","type":"facility","lineage":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]},{"id":"https://openalex.org/I98559091","display_name":"University of Rzesz\u00f3w","ror":"https://ror.org/03pfsnq21","country_code":"PL","type":"education","lineage":["https://openalex.org/I98559091"]}],"countries":["PL","UA"],"is_corresponding":true,"raw_author_name":"Andriy Sibirny","raw_affiliation_strings":["Department of Biotechnology and Microbiology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland","Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology and Microbiology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland","institution_ids":["https://openalex.org/I98559091"]},{"raw_affiliation_string":"Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine","institution_ids":["https://openalex.org/I149851306","https://openalex.org/I4210155364"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5048817862"],"corresponding_institution_ids":["https://openalex.org/I149851306","https://openalex.org/I4210155364","https://openalex.org/I98559091"],"apc_list":{"value":3200,"currency":"USD","value_usd":3200},"apc_paid":null,"fwci":0.0715,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.28710548,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"45","issue":"3","first_page":"528","last_page":"535"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.6201000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.6201000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.35010001063346863,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12688","display_name":"Microbial metabolism and enzyme function","score":0.00800000037997961,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fructose-1-6-bisphosphatase","display_name":"fructose-1,6-bisphosphatase","score":0.9909999966621399},{"id":"https://openalex.org/keywords/komagataella-phaffii","display_name":"Pichia pastoris","score":0.9819999933242798},{"id":"https://openalex.org/keywords/autophagy","display_name":"autophagy","score":0.9290000200271606},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.7910000085830688},{"id":"https://openalex.org/keywords/methylotrophic-yeast","display_name":"methylotrophic yeast","score":0.8420000076293945},{"id":"https://openalex.org/keywords/methanol-metabolism","display_name":"methanol metabolism","score":0.9670000076293945},{"id":"https://openalex.org/keywords/proteasome","display_name":"proteasome","score":0.6639999747276306},{"id":"https://openalex.org/keywords/peroxisomes","display_name":"peroxisomes","score":0.8330000042915344},{"id":"https://openalex.org/keywords/enzyme-inactivation","display_name":"enzyme inactivation","score":0.6340000033378601}],"concepts":[{"id":"https://openalex.org/C127078168","wikidata":"https://www.wikidata.org/wiki/Q138639","display_name":"Peroxisome","level":3,"score":0.7087612748146057},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.68708336353302},{"id":"https://openalex.org/C2776148510","wikidata":"https://www.wikidata.org/wiki/Q6468907","display_name":"Lactacystin","level":4,"score":0.6340076923370361},{"id":"https://openalex.org/C98539663","wikidata":"https://www.wikidata.org/wiki/Q220599","display_name":"Cytosol","level":3,"score":0.5987054109573364},{"id":"https://openalex.org/C2778506107","wikidata":"https://www.wikidata.org/wiki/Q3272916","display_name":"MG132","level":4,"score":0.564285397529602},{"id":"https://openalex.org/C189909181","wikidata":"https://www.wikidata.org/wiki/Q899507","display_name":"Fructose 1,6-bisphosphatase","level":3,"score":0.548521876335144},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5007038116455078},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.43932706117630005},{"id":"https://openalex.org/C27740335","wikidata":"https://www.wikidata.org/wiki/Q14878333","display_name":"Proteasome","level":2,"score":0.42153415083885193},{"id":"https://openalex.org/C102568950","wikidata":"https://www.wikidata.org/wiki/Q127702","display_name":"Vacuole","level":3,"score":0.41346442699432373},{"id":"https://openalex.org/C2776970464","wikidata":"https://www.wikidata.org/wiki/Q122043","display_name":"Fructose","level":2,"score":0.39865973591804504},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.39597922563552856},{"id":"https://openalex.org/C2778367456","wikidata":"https://www.wikidata.org/wiki/Q7251235","display_name":"Proteasome inhibitor","level":3,"score":0.3457812964916229},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32941320538520813},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10473743081092834},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000429","descriptor_name":"Alcohol Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000432","descriptor_name":"Methanol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001343","descriptor_name":"Autophagy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004718","descriptor_name":"Saccharomycetales","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D004718","descriptor_name":"Saccharomycetales","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D006597","descriptor_name":"Fructose-Bisphosphatase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D059748","descriptor_name":"Proteolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/cbin.11304","doi":"https://doi.org/10.1002/cbin.11304","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbin.11304","pdf_url":null,"source":{"id":"https://openalex.org/S7325069","display_name":"Cell Biology International","issn_l":"1065-6995","issn":["1065-6995","1095-8355"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Biology International","raw_type":"journal-article"},{"id":"pmid:31903651","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31903651","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell biology international","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2945082094","display_name":null,"funder_award_id":"Grant 31-19","funder_id":"https://openalex.org/F4320322850","funder_display_name":"National Academy of Sciences of Ukraine"}],"funders":[{"id":"https://openalex.org/F4320322850","display_name":"National Academy of Sciences of Ukraine","ror":"https://ror.org/00je4t102"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W44373803","https://openalex.org/W1772777894","https://openalex.org/W1775749144","https://openalex.org/W1938341712","https://openalex.org/W1968912788","https://openalex.org/W1969875733","https://openalex.org/W1973455824","https://openalex.org/W1978112795","https://openalex.org/W1991787623","https://openalex.org/W2018636808","https://openalex.org/W2019215631","https://openalex.org/W2020040718","https://openalex.org/W2041146932","https://openalex.org/W2047733018","https://openalex.org/W2048108455","https://openalex.org/W2067330538","https://openalex.org/W2085459881","https://openalex.org/W2097630079","https://openalex.org/W2109034822","https://openalex.org/W2151810319","https://openalex.org/W2340596933","https://openalex.org/W2415511385","https://openalex.org/W2475550688"],"related_works":["https://openalex.org/W7163053720","https://openalex.org/W7217095763","https://openalex.org/W3093154567","https://openalex.org/W3009587615","https://openalex.org/W4407624376","https://openalex.org/W4321601511","https://openalex.org/W6988188830","https://openalex.org/W2075430039","https://openalex.org/W150279473","https://openalex.org/W2105739087"],"abstract_inverted_index":{"Many":[0],"enzymes":[1,48,59],"of":[2,5,14,22,25,36,49,55,57,66,75,86,99,107,117,129,153,174,187,195],"methanol":[3,50],"metabolism":[4,51],"methylotrophic":[6,26],"yeasts":[7,27],"are":[8],"located":[9],"in":[10,33,71,95,120,131,140,148,216],"peroxisomes":[11,181],"whereas":[12,138],"some":[13],"them":[15],"have":[16,229],"the":[17,34,64,69,76,81,84,87,105,108,141,154,168,185,196,202],"cytosolic":[18,37,58],"localization.":[19],"After":[20],"shift":[21,116],"methanol-grown":[23,118],"cells":[24,119],"to":[28,135],"glucose":[29,121],"medium,":[30],"a":[31],"decrease":[32],"activity":[35,74],"(formaldehyde":[38],"dehydrogenase,":[39,41],"formate":[40],"and":[42,92,144,177,189],"fructose-1,6-bisphosphatase":[43],"[FBP])":[44],"along":[45],"with":[46,83,102,171],"peroxisomal":[47],"is":[52],"observed.":[53],"Mechanisms":[54],"inactivation":[56,128,139,160],"remain":[60],"unknown.":[61],"To":[62,163],"study":[63],"mechanism":[65],"FBP":[67,130,159,165],"inactivation,":[68],"changes":[70],"its":[72],"specific":[73],"wild":[77],"type":[78],"strain":[79,82,93],"GS200,":[80],"deletion":[85],"GSS1":[88],"hexose":[89],"sensor":[90],"gene":[91],"defected":[94],"autophagy":[96,214],"pathway":[97,215],"SMD1163":[98,143],"Komagataella":[100],"phaffii":[101,176,220],"or":[103],"without":[104],"addition":[106],"MG132":[109,157],"(proteasome":[110],"degradation":[111,137,166],"inhibitor)":[112],"were":[113,182],"investigated":[114],"after":[115],"medium.":[122],"Western":[123],"blot":[124],"analysis":[125,194],"showed":[126],"that":[127,210],"GS200":[132,188],"occurred":[133],"due":[134],"protein":[136],"strains":[142,170,198],"gss1\u0394":[145],"was":[146,161,199],"negligible":[147],"such":[149],"conditions.":[150],"The":[151,191],"effect":[152],"proteasome":[155],"inhibitor":[156],"on":[158,184],"insignificant.":[162],"confirm":[164],"pathway,":[167],"recombinant":[169],"GFP-labeled":[172],"Fbp1":[173,211],"K.":[175,217,219],"red":[178],"fluorescent":[179,192],"protein-labeled":[180],"constructed":[183,197],"background":[186],"SMD1163.":[190],"microscopy":[193],"performed":[200],"using":[201],"vacuolar":[203],"membrane":[204],"dye":[205],"FM4-64.":[206],"Microscopic":[207],"data":[208],"confirmed":[209],"degrades":[212],"by":[213],"phaffii.":[218],"transformants,":[221],"which":[222],"express":[223],"heterologous":[224],"\u03b2-galactosidase":[225],"under":[226],"FLD":[227],"promoter,":[228],"been":[230],"constructed.":[231]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2020-01-10T00:00:00"}