{"id":"https://openalex.org/W2996973038","doi":"https://doi.org/10.15616/bsl.2019.25.4.331","title":"Development of Reverse Transcription Semi-nested PCR Primer Pairs for the Specific and Highly Sensitive Detection of Human Aichivirus A1","display_name":"Development of Reverse Transcription Semi-nested PCR Primer Pairs for the Specific and Highly Sensitive Detection of Human Aichivirus A1","publication_year":2019,"publication_date":"2019-12-31","ids":{"openalex":"https://openalex.org/W2996973038","doi":"https://doi.org/10.15616/bsl.2019.25.4.331","mag":"2996973038"},"language":"en","primary_location":{"id":"doi:10.15616/bsl.2019.25.4.331","doi":"https://doi.org/10.15616/bsl.2019.25.4.331","is_oa":true,"landing_page_url":"https://doi.org/10.15616/bsl.2019.25.4.331","pdf_url":"http://www.bslonline.org/journal/download_pdf.php?doi=10.15616/BSL.2019.25.4.331","source":{"id":"https://openalex.org/S4210201443","display_name":"Journal of Experimental & Biomedical Sciences/Biomedical Science Letters","issn_l":"1738-3226","issn":["1738-3226","2288-7415"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["kci-registered"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Science Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"http://www.bslonline.org/journal/download_pdf.php?doi=10.15616/BSL.2019.25.4.331","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101496351","display_name":"Siwon Lee","orcid":"https://orcid.org/0000-0002-3442-9249","observed_orcids":["https://orcid.org/0000-0002-3442-9249"]},"institutions":[{"id":"https://openalex.org/I4210094357","display_name":"Shinhan University","ror":"https://ror.org/00sc0e019","country_code":"KR","type":"education","lineage":["https://openalex.org/I4210094357"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Siwon Lee","raw_affiliation_strings":["Department of Biomedical Laboratory Science, Shinhan University, Uijeongbu 11644, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Laboratory Science, Shinhan University, Uijeongbu 11644, Korea","institution_ids":["https://openalex.org/I4210094357"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5058503318","display_name":"Kyu Bong Cho","orcid":"https://orcid.org/0000-0002-2457-7811","observed_orcids":["https://orcid.org/0000-0002-2457-7811","https://orcid.org/0009-0009-4035-2637"]},"institutions":[{"id":"https://openalex.org/I4210094357","display_name":"Shinhan University","ror":"https://ror.org/00sc0e019","country_code":"KR","type":"education","lineage":["https://openalex.org/I4210094357"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Kyu Bong Cho","raw_affiliation_strings":["Department of Biomedical Laboratory Science, Shinhan University, Uijeongbu 11644, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Laboratory Science, Shinhan University, Uijeongbu 11644, Korea","institution_ids":["https://openalex.org/I4210094357"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5058503318"],"corresponding_institution_ids":["https://openalex.org/I4210094357"],"apc_list":null,"apc_paid":null,"fwci":0.7771,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.72472926,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"25","issue":"4","first_page":"331","last_page":"338"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10976","display_name":"Viral gastroenteritis research and epidemiology","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10976","display_name":"Viral gastroenteritis research and epidemiology","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11331","display_name":"Fecal contamination and water quality","score":0.004699999932199717,"subfield":{"id":"https://openalex.org/subfields/2312","display_name":"Water Science and Technology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nested-rt-pcr","display_name":"nested RT-PCR","score":0.8669999837875366},{"id":"https://openalex.org/keywords/primer-design","display_name":"primer design","score":0.8029999732971191},{"id":"https://openalex.org/keywords/virus-detection-sensitivity","display_name":"viral detection sensitivity","score":0.5830000042915344},{"id":"https://openalex.org/keywords/waterborne-viruses","display_name":"waterborne viruses","score":0.753000020980835},{"id":"https://openalex.org/keywords/kobuvirus","display_name":"kobuvirus","score":0.8659999966621399},{"id":"https://openalex.org/keywords/picornavirus","display_name":"picornavirus","score":0.9079999923706055}],"concepts":[{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.8102397322654724},{"id":"https://openalex.org/C157431215","wikidata":"https://www.wikidata.org/wiki/Q856465","display_name":"Nested polymerase chain reaction","level":4,"score":0.5364070534706116},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5102086067199707},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.46183112263679504},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4410875141620636},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.44065189361572266},{"id":"https://openalex.org/C2777116013","wikidata":"https://www.wikidata.org/wiki/Q6692995","display_name":"Low copy number","level":4,"score":0.44045117497444153},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.43041878938674927},{"id":"https://openalex.org/C4692481","wikidata":"https://www.wikidata.org/wiki/Q169526","display_name":"Reverse transcription polymerase chain reaction","level":4,"score":0.4106690287590027},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.38985273241996765},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.19731634855270386},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1924741268157959},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16972476243972778},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.09999993443489075},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.15616/bsl.2019.25.4.331","doi":"https://doi.org/10.15616/bsl.2019.25.4.331","is_oa":true,"landing_page_url":"https://doi.org/10.15616/bsl.2019.25.4.331","pdf_url":"http://www.bslonline.org/journal/download_pdf.php?doi=10.15616/BSL.2019.25.4.331","source":{"id":"https://openalex.org/S4210201443","display_name":"Journal of Experimental & Biomedical Sciences/Biomedical Science Letters","issn_l":"1738-3226","issn":["1738-3226","2288-7415"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["kci-registered"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Science Letters","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.15616/bsl.2019.25.4.331","doi":"https://doi.org/10.15616/bsl.2019.25.4.331","is_oa":true,"landing_page_url":"https://doi.org/10.15616/bsl.2019.25.4.331","pdf_url":"http://www.bslonline.org/journal/download_pdf.php?doi=10.15616/BSL.2019.25.4.331","source":{"id":"https://openalex.org/S4210201443","display_name":"Journal of Experimental & Biomedical Sciences/Biomedical Science Letters","issn_l":"1738-3226","issn":["1738-3226","2288-7415"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["kci-registered"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Science Letters","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6240000128746033}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1080666077","display_name":null,"funder_award_id":"S2661713","funder_id":"https://openalex.org/F4320327819","funder_display_name":"Ministry of SMEs and Startups"}],"funders":[{"id":"https://openalex.org/F4320327819","display_name":"Ministry of SMEs and Startups","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2996973038.pdf","grobid_xml":"https://content.openalex.org/works/W2996973038.grobid-xml"},"referenced_works_count":15,"referenced_works":["https://openalex.org/W149731399","https://openalex.org/W1520464544","https://openalex.org/W1886132146","https://openalex.org/W2020714235","https://openalex.org/W2041595020","https://openalex.org/W2095396545","https://openalex.org/W2099794932","https://openalex.org/W2121435478","https://openalex.org/W2124827597","https://openalex.org/W2148551427","https://openalex.org/W2153901817","https://openalex.org/W2164798354","https://openalex.org/W2165304918","https://openalex.org/W2170952152","https://openalex.org/W2908195214"],"related_works":["https://openalex.org/W3210622662","https://openalex.org/W4392761310","https://openalex.org/W2926429118","https://openalex.org/W4244105366","https://openalex.org/W4381415448","https://openalex.org/W4251726091","https://openalex.org/W4200207130","https://openalex.org/W2365538420","https://openalex.org/W2363826423","https://openalex.org/W3011297418"],"abstract_inverted_index":{"Human":[0],"Aichivirus":[1],"A1":[2],"(HuAiV-A1)":[3],"is":[4,113,133],"a":[5,19,55,83,124],"waterborne":[6],"human":[7],"pathogenic":[8],"virus":[9,128],"classified":[10],"as":[11,82,138],"Picornaviridae":[12],"and":[13,61,145],"Kobuvirus.In":[14],"this":[15,47,111,130],"study,":[16],"we":[17],"developed":[18,45,109],"method":[20,71,89,96,106,132],"that":[21],"can":[22],"detect":[23],"about":[24,58],"35":[25],"minutes":[26],"faster":[27],"with":[28,97],"the":[29,35,68,79,95,98,104,119],"same":[30],"detection":[31,85,100,105,131],"sensitivity":[32,86,101],"level":[33,56],"than":[34,94],"previously":[36],"reported":[37],"HuAiV-A1":[38,53,108,139],"diagnostic":[39,140],"RT-PCR":[40,42,80],"primer.The":[41],"primer":[43],"sets":[44],"in":[46,110,118,122,142],"study":[48,112],"are":[49],"capable":[50],"of":[51,57,87,107,127],"detecting":[52],"at":[54],"100":[59],"ag":[60],"formed":[62],"563":[63],"bp":[64,77],"amplification":[65],"product.In":[66],"addition,":[67],"RT-nested":[69],"PCR":[70],"was":[72,90],"able":[73],"to":[74,102,115,135],"amplify":[75],"410":[76],"using":[78],"product":[81],"template.The":[84],"our":[88],"10":[91],"times":[92],"higher":[93],"highest":[99],"date.Therefore,":[103],"expected":[114,134],"be":[116,136],"used":[117,137],"water":[120],"environment":[121],"which":[123],"small":[125],"amount":[126],"exists.Also,":[129],"technology":[141],"both":[143],"clinical":[144],"non-clinical":[146],"field.":[147]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1}],"updated_date":"2026-09-24T07:57:37.273135","created_date":"2020-01-10T00:00:00"}